ACRF Biomolecular Resource Facility (the BRF)
John Curtin School of Medical Research
BRF Phone:
BRF Email:
BRF Fax:
Web:
+61 2 6125 4326
brf@anu.edu.au
+61 2 6125 9533
www.brf.anu.edu.au
GDU Phone: +61 2 6125 9289
GDU Email: jcsmr.gdu@anu.edu.au
Sample Drop-off Address:
L2, Building 131, Garran Road
Australian National University
Post: GPO Box 334
Canberra ACT 2601
Please print this form and complete all
information requested. Contact us if
unsure. This form MUST be signed by
PI / Lab Head. Bring it to BRF with your
samples.
HiSeq 2500 Order Form
Sequencing with Customer-made Library
Part 1: Contact Information
Part 2: Billing Information
Date:
Customer name:
ANU Customers:
Please provide ANU account code.
Non ANU Customers:
Customer address:
Phone (lab):
Phone (mobile):
Email:
A tax invoice will be emailed to the PI
(or Lab Head) unless alternative
information is provided here.
Part 3: Output
Please choose a data option
Analysed by GDU

(Please contact jcsmr.gdu@anu.edu.au or 6125 9289 for a
discussion of method, and for pricing).
PI (or Lab Head) name:
PI (or Lab Head) email:
PI (or Lab Head) signature:


Purchase 2TB hard drive from BRF ($170)
Supply your own hard drive
By signing, you acknowledge and
accept BRF charges, and terms and
conditions.
Page 1 of 3
Part 4: Libraries and sequence (Customer-made libraries)
Please provide 25 L of 2nM DNA for each lane.
The recommended method to quantify libraries is Bioanalyzer. Bring your Bioanalyzer results with your samples.
A.
Experimental design
B.
Library construction method. Please complete either (a) or (b)
Library Kit Name
(a) Illumina Method
(b) Non Illumina Method
Part Number
Lot Number
Please attach the protocol you used to this form. Customer designed adapter
and primer sequence need to be included.
 Protocol attached.
C. Sample Information. Please change this table to suit your experimental design.
Sample Submission Date:
Lane
Example
Lane 1
1.
2.
3.
4.
Sample Name
Index*
Sample Origin (species/tissue)
Sequence Length
BRF 1
BRF 2
BRF 3
BRF 4
ATCACG
PCR primer index 1
GGCTAC
Truseq index 11
Mouse liver genomic DNA
Mouse blood DNA
Mouse genomic DNA exon capture
Mouse muscle genomic DNA
100 bp PE
1.
2.
3.
4.
Page 2 of 3
Part 4 (continued)
Lane 2
Lane 3
Lane 4
Lane 5
Lane 6
1.
2.
3.
4.
1.
2.
3.
4.
1.
2.
3.
4.
1.
2.
3.
4.
1.
2.
3.
4.
*Index can be the index sequence or the index name if you use the standard Illumina kit. Please note: Illumina does not support longer read lengths than below.
For your reference, sequence length can be:
Single Read
Paired-end Read
50 base single read (50 bp SR)
50 base paired-end read (75 bp PE)
100 base single read (100 bp SR)
100 base paired-end read (100 bp PE)
150 base single read (150 bp SR)
150 base paired-end read (150 bp PE)
Page 3 of 3