ACRF Biomolecular Resource Facility John Curtin School of Medical Research BRF Phone: BRF Email: BRF Fax: Web: +61 2 6125 4326 brf@anu.edu.au +61 2 6125 9533 www.brf.anu.edu.au GDU Phone: +61 2 6125 9289 GDU Email: jcsmr.gdu@anu.edu.au Sample Drop-Off Address: L2, Building 131, Garran Rd Australian National University Post: GPO Box 334, Canberra ACT 2601 Please print this form and complete all information requested. Contact us if unsure. This form MUST be signed by PI/Lab Head. Bring it to BRF with your samples. Hi Seq 2500 Order Form BRF-made Library Part 1: Contact Information Part 2: Billing Information Date: Customer name: ANU Customers: Please provide ANU account code. Customer address: Phone (lab): Phone (mobile): Email: PI (or Lab Head) name: PI (or Lab Head) email: PI (or Lab Head) signature: By signing, you acknowledge and accept BRF charges, and terms and conditions. Non ANU Customers: A tax invoice will be emailed to the PI (or Lab Head) unless alternative information is provided here. Part 3: Output Please choose a data option Analysed by GDU (Please contact jcsmr.gdu@anu.edu.au or 6125 9289 for a discussion of method, and for pricing). Purchase 2TB hard drive from BRF ($170) Supply your own hard drive Page 1 of 3 Part 4: Libraries and sequence (BRF makes the library) Please supply your gel picture or Bioanalyzer results. A. Experimental design B. Sample Information Sample Submission Date: Sample Number Sample Name Sample Origin (species/tissue) Sample Type (DNA, RNA, etc) Bioanalyser RIN (for RNA) OD 260/280 OD 260/230 Sample Volume L Concentration (ng/L) For your reference: Illumina HiSeq Genomic DNA Sequence Sample ChIP Sequence Sample Small RNA mRNA Sequence Sample Mate Pair Sequence Nextera / Nextera XT Sample 1 Sample 2 Input Material Required 3g of Genomic DNA 125ng of ChIP DNA 3g of total RNA 3g of total RNA 3ug-gel free; 6-8ug-gel method Contact BRF Sample 3 Conc’n (ng/L) 100 5 100 100 100 Sample 4 Sample 5 Volume (L) 30 25 30 30 30 C. Library Type (please tick one) gDNA Exome mRNA Stranded Small Haloplex capture RNA library RNA If you have any special library requirements, please describe them here: RIN N/A N/A >8 >8 Mate pair Sample 6 OD 260/280 1.8 – 2.1 1.8 – 2.1 1.8 – 2.1 1.8 – 2.1 1.8 – 2.1 1.8 – 2.1 ChIP library Sample 7 OD 260/280 >1.5 >1.5 >1.5 >1.5 >1.5 >1.5 Nextera/XT Page 2 of 3 Part 4 continued D. Sequencing Information. Lane Sequence length No. of Sample Sample Name (Samples with a different index can be mixed and run on one lane) Example BRF 1, BRF 2, BRF 3, BRF 5, BRF 12 75 bp PE 5 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 For your reference, sequence length can be: Please note: Illumina does not support longer read lengths than below. Single Read Paired-end Read 50 base single read (50 bp SR) 50 base paired-end read (75 bp PE) 100 base single read (100 bp SR) 100 base paired-end read (100 bp PE) 150 base single read (150bp SR) 150 base paired-end read (150 bp PE) Page 3 of 3