ACRF Biomolecular Resource Facility
John Curtin School of Medical Research
BRF Phone:
BRF Email:
BRF Fax:
Web:
+61 2 6125 4326
brf@anu.edu.au
+61 2 6125 9533
www.brf.anu.edu.au
GDU Phone: +61 2 6125 9289
GDU Email: jcsmr.gdu@anu.edu.au
Sample Drop-Off Address:
L2, Building 131, Garran Rd
Australian National University
Post: GPO Box 334,
Canberra ACT 2601
Please print this form and complete
all information requested. Contact us
if unsure. This form MUST be signed by
PI/Lab Head. Bring it to BRF with your
samples.
Hi Seq 2500 Order Form
BRF-made Library
Part 1: Contact Information
Part 2: Billing Information
Date:
Customer name:
ANU Customers:
Please provide ANU account code.
Non ANU Customers:
Customer address:
Phone (lab):
Phone (mobile):
Email:
PI (or Lab Head) name:
PI (or Lab Head) email:
PI (or Lab Head) signature:
By signing, you acknowledge and
accept BRF charges, and terms and
conditions.
A tax invoice will be emailed to the PI
(or Lab Head) unless alternative
information is provided here.
Part 3: Output


Analysed by GDU
(Please contact jcsmr.gdu@anu.edu.au or 6125 9289 for a
discussion of method, and for pricing).
Purchase 2TB hard drive from BRF ($170)
Supply your own hard drive. Please bring a HD at
the same time as you submit your samples. Label
with your name, research group and phone number.
*Note: Customers must keep a backup of their own data, so
please either supply a hard drive or purchase one from the BRF.

Page 1 of 3
Part 4: Libraries and sequence (BRF makes the library)
Please supply your gel picture or Bioanalyzer results.
A.
Experimental design
B.
Sample Information
Sample Submission Date:
Sample Number
Sample Name
Sample Origin (species/tissue)
Sample Type (DNA, RNA, etc)
Bioanalyser RIN (for RNA)
OD 260/280
OD 260/230
Sample Volume L
Concentration (ng/L)
For your reference:
Illumina HiSeq
Genomic DNA Sequence Sample
ChIP Sequence Sample
Small RNA
mRNA Sequence Sample
Mate Pair Sequence
Nextera / Nextera XT
Sample 1
Sample 2
Input Material Required
3g of Genomic DNA
125ng of ChIP DNA
3g of total RNA
3g of total RNA
3ug-gel free; 6-8ug-gel method
Contact BRF
Sample 3
Conc’n (ng/L)
100
5
100
100
100
Sample 4
Sample 5
Volume (L)
30
25
30
30
30
C. Library Type (please tick one)
 gDNA
 Exome
 mRNA
 Stranded  Small
 Haloplex
capture
RNA library RNA
If you have any special library requirements, please describe them here:
RIN
N/A
N/A
>8
>8
 Mate pair
Sample 6
OD 260/280
1.8 – 2.1
1.8 – 2.1
1.8 – 2.1
1.8 – 2.1
1.8 – 2.1
1.8 – 2.1
 ChIP library
Sample 7
OD 260/280
>1.5
>1.5
>1.5
>1.5
>1.5
>1.5
 Nextera/XT
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Part 4 continued
D. Sequencing Information.
Lane
Sequence length No. of Sample
Sample Name (Samples with a different index can be mixed and run on one lane)
Example
BRF 1, BRF 2, BRF 3, BRF 5, BRF 12
75 bp PE
5
Flowcell
1
Flowcell
2
Flowcell
3
Flowcell
4
Flowcell
5
For your reference, sequence length can be:
Single Read
50 base single read (50 bp SR)
100 base single read (100 bp SR)
150 base single read (150bp SR)
Paired-end Read
50 base paired-end read (75 bp PE)
100 base paired-end read (100 bp PE)
150 base paired-end read (150 bp PE)
250 base paired-end read (250 bp PE)
Please note: It is the responsibility of customers to keep a backup of their data, even if that data is being analysed by the GDU. All data
must be checked by the customer within 2 weeks of receiving it. Any problems must be reported to the BRF within that time period.
Page 3 of 3