ACRF Biomolecular Resource Facility
John Curtin School of Medical Research
BRF Phone:
BRF Email:
BRF Fax:
Web:
+61 2 6125 4326
brf@anu.edu.au
+61 2 6125 9533
www.brf.anu.edu.au
GDU Phone: +61 2 6125 9289
GDU Email: jcsmr.gdu@anu.edu.au
Sample Drop-Off Address:
L2, Building 131, Garran Rd
Australian National University
Post: GPO Box 334,
Canberra ACT 2601
Please print this form and complete
all information requested. Contact us
if unsure. This form MUST be signed by
PI/Lab Head. Bring it to BRF with your
samples.
MiSeq Order Form
BRF-made Library
Part 1: Contact Information
Part 2: Billing Information
Date: (Must be filled in)
Customer name:
ANU Customers:
Please provide ANU account code.
Customer address:
Phone (lab):
Phone (mobile):
Email:
PI (or Lab Head) name:
PI (or Lab Head) email:
PI (or Lab Head) signature:
By signing, you acknowledge and
accept BRF charges, and terms and
conditions.
Non ANU Customers:
A tax invoice will be emailed to the PI
(or Lab Head) unless alternative
information is provided here.
Part 3: Output
Please choose a data option
Analysed by GDU



(Please contact jcsmr.gdu@anu.edu.au or 6125 9289 for a
discussion of method, and for pricing).
Purchase 2TB hard drive from BRF ($170)
Supply your own hard drive
Page 1 of 3
Part 4: Libraries and sequence (BRF makes the library)
Please supply your gel picture or Bioanalyzer results.
A.
Experimental design
B.
Sample Information
Sample Submission Date:
Sample Number
Sample Name
Sample Origin (species/tissue)
Sample Type (DNA, RNA, etc)
Bioanalyser RIN (for RNA)
OD 260/280
OD 260/230
Sample Volume L
Concentration (ng/L)
For your reference:
Illumina HiSeq
Genomic DNA library (large genomes)
Nextera DNA library (small
genomes/amplicons)
Small RNA
mRNA Sequence Sample
Other
Sample 1
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
Sample 7
Input Material Required
3g of Genomic DNA
Conc’n (ng/L)
100
5
Volume (L)
30
RIN
N/A
N/A
OD 260/280
1.8 – 2.1
1.8 – 2.1
OD 260/280
>1.5
>1.5
3g of total RNA
3g of total RNA
Contact BRF
100
100
30
30
>8
>8
1.8 – 2.1
1.8 – 2.1
>1.5
>1.5
C. Library Type (please tick one)
 Genomic library
 Exome capture
 mRNA library
 Small RNA library
If you have any special library requirements, please describe them here:
 Nextera
 Other
Page 2 of 3
Part 4 continued
D. Sequencing Information.
Lane
Sequence length No. of Sample
Example
Standard Flowcell,
150bp paired end
5
Sample Name (Samples with a different index can be mixed and run on the Flowcell)
BRF 1, BRF 2, BRF 3, BRF 5, BRF 12
Flowcell
For your reference, sequence length can be:
Standard Flowcell about 15M
Nano Flowcell about 1M clusters
clusters
50 cycles (50 bp Single Read) V2
300 cycles (150 bp Paired End) V2
300 cycles (150 bp Paired End) V2
500 cycles (250bp Paired End) V2
500 cycles (250bp Paired End) V2
Micro Flowcell about 4M clusters
300 cycles (150 bp Paired End) V2
Standard Flowcell about 25M
clusters
150 cycles (75bp Paired End) V3
600 cycles (300bp Paired end) V3
Page 3 of 3