OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Paratuberculosis
Address of laboratory
Veterinary Research Institute, Hudcova 70, 621 00
Brno, Czech Republic
Tel.:
(+420) 5 3333 1616
Fax:
(+420) 5 3333 1229
e-mail address:
pavlik@vri.cz
website:
http://www.vri.cz/en/departments/laboratoryoie_reference_laboratory_for_paratuberculosis
Name (including Title) of Head of
Laboratory (Responsible Official):
Professor Ivo Pavlik, Head of the Deparatment
Food and Feed Safety
Name (including Title and Position) of OIE
Reference Expert:
Professor Ivo Pavlik, Head of the Deparatment
Food and Feed Safety
Date of submission to the OIE
27.1.2013
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Paratuberculosis » – « Ivo Pavlik » – « Czech Republic »
Test recommended by the OIE
Indirect diagnostic tests
Internationally
0 tests
from cattle, 664 blood
samples from goats)
Direct diagnostic tests
Culture examination with HPCsedimentation method, 2085 tests
Quantitative Real Time PCR (F57 and
IS9001), 2077 tests
Nationally
Internationally
2085 tests (1 750 cattle
faeces, 46 cattle tissues, 16
cow’s milk samples, 95 wild
ruminant faeces, 77 wild
ruminant tissues and 101
environmental samples)
2077 tests (263 tissues, 1539
faeces, 73 milk samples, 42
milk filters and 160
environmental samples)
0 tests
0 tests
Specific fragments for M. avium subsp. paratuberculosis detection; home-made technique: Slana, I., Kralik,
P., Kralova, A., Pavlik, I.: On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw
milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination.
International Journal of Food Microbiology, 2008, 128 (2), 250-257.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
4.
x No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
9.
x No
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
x No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
7.
x No
Did your laboratory produce vaccines?
Yes
6.
x No
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
x No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
x No
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
2
Nationally
4409 tests (3745 blood samples
ELISA 4409
1
Total number of test performed last year
x No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Paratuberculosis » – « Ivo Pavlik » – « Czech Republic »
10.
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
11.
x No
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
12.
x No
Did your Laboratory collect epizootiological data relevant to international disease control?
x Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
x Yes
14.
15.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: .................
6
b)
International conferences: ..............................................
7
c)
National conferences: ......................................................
4
d)
Other: ...............................................................................
internet, invited lectures
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
16.
x No
Does your laboratory have a Quality Management System certified according to an International
Standard?
x Yes
No
Quality management system adopted
ISO 17025:
Ziehl-Neelsen staining
Culture examination
qPCR for the detection of F57 and IS900
17.
Is your laboratory accredited by an international accreditation body?
Yes
x No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
x No
x No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Annual reports of OIE Reference Laboratories, 2012
x No
3
OIE RL for « Paratuberculosis » – « Ivo Pavlik » – « Czech Republic »
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
x No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
x No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
24.
x No
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
25.
x No
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
a)
x No
Articles published in peer-reviewed journals
Slana, I., Kralik, P., Kralova, A., Babak, V., Pavlik, I.: Examination of milk filters by real-time PCR as a herd-level
indicator for the presence of Mycobacterium avium subsp. paratuberculosis in dairy herds. Journal of
Dairy Science, 2012, 95 (3), 1162-1165. http://dx.doi.org/10.3168/jds.2011-4658
Klanicova, B., Slana, I., Roubal, P., Pavlik, I., Kralik, P.: Mycobacterium avium subsp. paratuberculosis survival
during fermentation of soured milk products detected by culture and quantitative real time PCR
methods. International Journal of Food Microbiology, 2012, 157, 150-155.
Moravkova, M., Babak, V., Kralova, A., Pavlik, I., Slana, I.: Culture- and quantitative IS900 real-time PCR based
analysis of the persistence of Mycobacterium avium subsp. paratuberculosis in a controlled dairy
cow farm environment. Applied and Environmental Microbiology, 2012, 78 (18), 6608-6614. DOI:
10.1128/AEM.01264-12
Pribylova, R., Kubickova, L., Babak, V., Pavlik, I., Kralik, P.: Effect of short- and long-term antibiotic exposure
on the viability of Mycobacterium avium subsp. paratuberculosis as measured by propidium
monoazide F57 real time quantitative PCR and culture. The Veterinary Journal, 2012, 194, 354-360.
http://dx.doi.org/10.1016/j.tvjl.2012.05.002.
Hruska, K., Kaevska M.: Mycobacteria in water, soil, plants and air: a review. Veterinarni Medicina, 2012, 57,
623–679. http://vri.cz/docs/vetmed/57-12-623.pdf
Kralik, P., Beran, V., Pavlik, I.: Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative
real-time PCR, culture on solid media and optical densitometry. BMC Research notes, 2012, 5, 114.
http://www.biomedcentral.com/1756-0500/5/114
b) International conferences
Kralik, P., Slana, I., Kralova, A., Babak, V., Kovarcik, K., Pavlik, I.: Control of paratuberculosis in the closed herd
of cattle by real time PCR: Does passive shedding really exists? In: Proceedings of the 11th
International Colloquium on Paratuberculosis, 5 - 10 February, 2012, Sydney, Australia, 42.
4
Annual reports of OIE Reference Centres, 2012
OIE RL for « Paratuberculosis » – « Ivo Pavlik » – « Czech Republic »
Kralik, P., Beran, V., Pavlik, I.: Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative
real time PCR, culture on solid media and optical densitometry. In: Proceedings of the 11th
International Colloquium on Paratuberculosis, 5 - 10 February, 2012, Sydney, Australia, 44.
Pavlik, I., Slana, I., Kralova, A., Kralik, P.: Paratuberculosis control in the Czech Republic: 25 years of
experience. In: Proceedings of the 11th International Colloquium on Paratuberculosis, 5 - 10
February, 2012, Sydney, Australia, 196.
Kralik, P., Pavlik, I.: Influence of repeated cycles of mechanical and physical disinfection procedures on
viability of Mycobacterium avium subsp. paratuberculosis measured by propidium monoazide F57
quantitative real time PCR. In: Proceedings of the 11th International Colloquium on Paratuberculosis,
5 - 10 February, 2012, Sydney, Australia, 214.
Pribylova, R., Kubickova, L., Babak, V., Pavlik, I., Kralik, P.: The effect of short- and long-term antibiotics
exposition on viability of Mycobacterium avium subsp. paratuberculosis measured by propidium
monoazide F57 realt time quantitative PCR and culture. In: Proceedings of the 11th International
Colloquium on Paratuberculosis, 5 - 10 February, 2012, Sydney, Australia, 227.
Kralik, P., Pavlik, I.: Influence of repeated cycles of mechanical and physical disinfection procedures on
viability of Mycobacterium avium subsp. paratuberculosis measured by propidium monoazied F57
quantitative real time PCR. In: Proceedings of the 11th International Colloquium on Paratuberculosis,
5 - 10 February, 2012, Sydney, Australia, 227.
Pavlik, I., Kriz, P., Hasonova, L.: Paratuberculosis control: diagnostic possibilities and the impact of the
diseases prevalence. In: Infektionskrankheiten des Rindes - Neues und Wichtiges für die tierärztliche
Praxis. University of Veterinary Medicine Hannover, Germany, 7.-8.9.2012, 83-95.
c)
National conferences
Klanicová, B., Seďa, J., Slaná, I., Kantorová, K., Slaný, M., Pavlík, I.: Sledování výskytu zástupců Mycobacterium
avium v sedimentech údolních nádrží, v úpravnách vod a v domácnostech v ČR. In: Slezské dny
preventivní medicíny, Karviná, 7.-9.3.2012, ISBN 978-80-7329-312-3, CD, 1 s.
Klanicová, B., Slaná, I., Roubal, P., Pavlik, I., Králík, P.: Sledování přežívání druhu Mycobacterium avium subsp.
paratuberculosis ve fermentovaných mléčných výrobcích pomocí metod kultivace a real time PCR. In:
Sborník abstraktů XXI. konference mladých mikrobiologů „Tomáškovy dny 2012“, 2.-3.6.2011 Brno,
ISBN 978-80-210-5874-3, 89-90.
Klanicová, B., Seďa, J., Slaná, I., Kantorová, K., Slaný, M., Pavlík, I.: Detekce druhu Mycobacterium avium
v sedimentech nádrží s pitnou vodou, v úpravnách vod a v domácnostech v ČR. XIV. Konference
mladých vědeckých pracovníků, Veterinární a farmaceutická univerzita, Brno, 29.5.2012, ISBN 97880-7305-612-4, 120-122.
Lorencová, A., Slaná, I., Vašíčková, P., Pavlik, I.: Sledování viru hepatitidy E a potenciálně patogenních
zástupců Mycobacterium avium subsp. v syrových masných produktech a užití porcinního adenoviru
k indikaci fekální kontaminace. In: 25. Pečenkovy dny, ISBN 978-80-904667, Harrachov, 18.20.9.2012, 102.
d) Other: ....................................................................................... internet, invited lectures
Knowledge dissemination is an important activity of the OIE Reference Laboratories for Avian Tuberculosis,
and for paratuberculosis, supported by the Veterinary Research Institute, Brno, Czech Republic and
Biomedical Technology, Epidemiology and Food Safety Global Network. Databases on paratuberculosis and
Crohn’s disease digest were updated in 2012 with a total of 374 new scientific articles (236 and 138,
respectively), published in peer reviewed journals and indexed in the Web of Science® (Thomson Reuters)
database. Members of the Biomedical Technology, Epidemiology and Food Safety Global Network were
informed about new publications by e-mails in weekly intervals. Both databases are available at the web page
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Paratuberculosis » – « Ivo Pavlik » – « Czech Republic »
of the OIE Reference Laboratory for Paratuberculosis, hosted by the Veterinary Research Institute, Brno.
Network members from 25 countries (Argentina, Australia, Bangladesh, Canada, Chile, Czech Republic,
Denmark, Egypt, France, Germany, India, Ireland, Italy, Nigeria, Portugal, Republic of Korea, Saudi Arabia,
Slovakia, Slovenia, Spain, Switzerland, The Netherlands, Turkey, UK and USA) requested papers from those on
offer. In total, 60 new items of information on mycobacteria were distributed by e-mail to network members
as the Centaur Global Network Information and were simultaneously archived at the network web page. This
open access system means that all information is available not only for the Centaur Global Network
members. Everybody can register as a person, interested in any or all of the 27 fields of interest, one of which
is (08) Mycobacterial diseases. In total, 257 e-mail notifications were dispatched in 2012, mostly to
distribution lists (27) Scientific information, (23) Education, (05) Zoonoses, (04) Food borne diseases and (08)
Mycobacterial diseases. The distance learning course on Good Research Practice was run also in 2012 and
the course assigned GRP 2013 has been opened in October 2012 and has to be finished by 31 March 2013.
The running course has registered 43 participants from 13 countries (Bangladesh, Belarus, Canada, Croatia,
Czech Republic, Ethiopia, India, Iran, Nigeria, Poland, Serbia, Slovakia, Yemen).
URL addresses of hyperlinks:
OIE Reference Laboratories for Avian Tuberculosis
http://www.vri.cz/en/departments/laboratory-oie_reference_laboratory_for_avian_tuberculosis
Veterinary Research Institute, Brno
http://www.vri.cz
Biomedical Technology, Epidemiology and Food Safety Global Network (CENTAUR)
http://centaur.vri.cz
registration on line
http://centaur.vri.cz/?page=registration
Database on paratuberculosis
http://www.vri.cz/en/publications/paratuberculosis
Database on Crohn’s disease digest
http://www.vri.cz/en/publications/cd_and_map
OIE Reference Laboratory for Paratuberculosis,
http://www.vri.cz/en/departments/laboratory-oie_reference_laboratory_for_paratuberculosis
distance learning course on Good Research Practice
http://centaur.vri.cz/?page=grp_e-course
GRP 2011 Report
http://centaur.vri.cz/docs/GRP2011/BULLETIN.pdf
Centaur Global Network Information
http://centaur.vri.cz/?page=cgn_information
Invited speaker as OIE expert for paratuberculosis
Pavlik, I.: Paratuberculosis in cows - epidemiological situation in central Europe. Chamber of Veterinary
Surgeons of Slovak Republic. In: Conference „Paratuberculosis in cattle“, Nitra, Slovakia, 6.3.2012.
Pavlik, I., Hruska, K.: Links between mycobacteria and Crohn's disease: food safety issues. 12th International
Nutrition and Diagnostics Conference 2012 (INDC2012), Praha, 27.-30.8.2012, ISBN 978-80-7395456-7, 52.
Pavlik, I., Kriz, P., Hasonova, L.: Paratuberkulose: diagnostische Optionen und Bedeutung der Prävalenz für die
Bekämpfungsstrategie (Paratuberculosis control: diagnostic possibilities and the impact of the
diseases prevalence). In: Infektionskrankheiten des Rindes - Neues und Wichtiges für die tierärztliche
Praxis. University of Veterinary Medicine Hannover, Germany, 7.9.2012, 83-95.
Pavlik I.: Paratuberculosis: epidemiology, diagnostic, prevention and control. Seminář: Le Laboratoire
Départemental D'analyses Du Conseil Général De La Creuse, En Collaboration Avec Le Groupement
De Défense Sanitaire De La Creuse Et Lsi - Life Technologies. La Souterraine, France, 23.10.2012.
6
Annual reports of OIE Reference Centres, 2012