OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Avian tuberculosis
Address of laboratory
Veterinary Research Institute, Hudcova 70, 621 00
Brno, Czech Republic
Tel.:
(+420) 5 3333 1616
Fax:
(+420) 5 3333 1229
e-mail address:
pavlik@vri.cz
website:
http://www.vri.cz/en/departments/laboratoryoie_reference_laboratory_for_avian_tuberculosis
Name (including Title) of Head of
Laboratory (Responsible Official):
Professor Ivo Pavlik, Head of the Department
Food and Feed Safety
Name (including Title and Position) of OIE
Reference Expert:
Professor Ivo Pavlik, Head of the Department
Food and Feed Safety
Date of submission to the OIE
27.1.2013
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
1.
Test(s) in use/or available for the specified disease at your laboratory
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
Rapid agglutination (Corpuscular antigen of
M. avium subsp. avium, serotype 2;
corpuscular antigen of M. avium subsp.
hominissuis, serotype 8 and corpuscular
antigen of M. intracellulare, serotype 19)
173 tests
173 tests (79 wild swine, 65
domestic pigs, 17 antelopes, 3
macaques, 2 dogs, 1 cat, 1
horse, 5 birds)
0 tests
Direct diagnostic tests
Nationally
Internationally
Culture examination (conventional culture
technique, media without Mycobactin J)
193 tests
193 tests (66 fish tissues, 1
poikilothermic animal tissue,
2 bird tissues, 2 bird faeces, 4
human tissues, 4 human
environment, 13 other
mammal tissues, 1 water
environment, 31 pigs
environment, 34 vegetable
samples, 35 meat products
0 tests
Quantitative Real Time PCR (M. a. avium
and M. a. hominissuis detected by the
presence/absence of IS1245 and IS9011)
338 tests
338 tests (220 tissues, 42
faeces and 76 environmental
samples)
0 tests
16S rDNA sequencing (Differentiation of
Mycobacterium sp. Harmsen et al.2 and
Telenti et al.3)
136 isolates (9 human, 53
animal and 61 environmental
isolates)
0 isolates
1
Specific fragments for M. avium subsp. avium and M. avium subsp. hominissuis ; home-made technique,
Slana, I., Kaevska, M., Kralik, P., Horvathova, A., Pavlik, I.: Distribution of Mycobacterium avium
subsp. avium and M. a. hominissuis in artificially infected pigs studied by culture and IS901 and
IS1245 quantitative Real Time PCR. Veterinary Microbiology, 2010, 144, 437-443.
2
Harmsen, D., Dostal, S., Roth, A., Niemann, S., Rothganger, J., Sammeth, M., Albert, J., Frosch, M., Richter, E.,
11-11-2003. RIDOM: comprehensive and public sequence database for identification of
Mycobacterium species. BMC Infection Diseases, 2003, 3, 26.
3
Telenti, A., Marchesi, F., Balz, M., Bally, F., Böttger, E.C. and Bodmer, T. Rapid identification of mycobacteria
to the species level by polymerase chain reaction and restriction enzyme analysis. Journal of Clinical
Microbiology, 1993, 31 (2), 175-178.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
4.
x No
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
2
x No
x No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
5.
Did your laboratory produce vaccines?
Yes
6.
Did your laboratory supply vaccines to OIE Member Countries?
Yes
7.
x No
x No
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
x Yes
8.
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
Name of the new test or diagnostic
method or vaccine developed
IS901 qPCR
9.
Description and References (Publication, website, etc.)
x No
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
x No
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
12.
x No
Did your Laboratory collect epizootiological data relevant to international disease control?
x Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
x Yes
14.
No
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
11.
x
Slana et al. (2010). Distribution of Mycobacterium avium
subsp. avium and M. a. hominissuis in artificially infected
pigs studied by culture and IS901 and IS1245 quantitative
real time PCR. Vet. Microbiol., 144, 437-443.
Yes
10.
No
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 7
b)
International conferences: .............................................. 5
c)
National conferences: ...................................................... 7
d)
Other: ............................................................................... internet, see Annex
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
16.
x No
Does your laboratory have a Quality Management System certified according to an International
Standard?
x Yes
No
Quality management system adopted
ISO 17025:
Ziehl-Neelsen staining
Culture examination
qPCR for the IS901 detection
17.
Is your laboratory accredited by an international accreditation body?
Yes
x No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
x No
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
4
x No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
24.
x No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
x No
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
x No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
21.
x No
x No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
a)
x No
Articles published in peer-reviewed journals
Faldyna, M., Gopfert, E., Kudlackova, H., Stepanova, H., Kaevska, M., Slana, I., Pavlik, I.: Usability of a gamma
interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subsp.
hominissuis. Journal of Veterinary Diagnostic Investigation, 2012, 24 (2), 376-378.
Skoric, M., Mrlik, V., Svobodova, J., Beran, V., Slany, M., Fictum, P., Pokorny, J., Pavlik, I.: Infection in a
Komodo dragon (Varanus komodoensis) caused by Mycobacterium intracellulare: a case report.
Veterinarni Medicina, 2012, 57 (3), 163-168. http://www.vri.cz/docs/vetmed/57-3-163.pdf
Matlova, L., Kaevska, M., Moravkova, M., Beran, V., Shitaye, J.E., Pavlik, I.: Mycobacteria in peat used as a
supplement for pigs: failure of different decontamination methods to eliminate the risk. Veterinarni
Medicina, 2012, 57 (4): 212-217. http://vri.cz/docs/vetmed/57-4-212.pdf
Slany, M., Pavlik, I.: Molecular detection of nontuberculous mycobacteria: advantages and limits of a broadrange sequencing approach. Journal of Molecular Microbiology and Biotechnology, 2012, 22, 268276. DOI:10.1159/000342517
Stepanova, H., Pavlova, B., Stromerova, N., Ondrackova, P., Stejskal, K., Slana, I., Zdrahal, Z., Pavlik, I., Faldyna,
M.: Different immune response of pigs to Mycobacterium avium subsp. avium and Mycobacterium
avium subsp. hominissuis infection. Infection and Immunity, 2012, 159, 343-350.
Klanicova, B., Lorencova, A. Makovcova, J., Vlkova, H., Kralik, P., Pavlik, I., Slany, M.: Survival of three
Mycobacterium avium subsp. hominissuis isolates in fish products after hot smoking and frying.
International Journal of Food Science and Technology, 2012, 1-6. doi:10.1111/j.1365-2621.03215.x
Hruska, K., Kaevska M.: Mycobacteria in water, soil, plants and air: a review. Veterinarni Medicina, 2012, 57,
623–679. http://vri.cz/docs/vetmed/57-12-623.pdf
b) International conferences
Slany, M., Slana, I., Ulmann. V., Pavlik, I.: Molecular analysis of human isolates belonging to Mycobacterium
avium complex collected during the years 2003-2009 in the Czech Republic. In: European Congress of
Clinical Microbiology and Infectious Diseases (ECCMID) 2012, 31.3-3.4.2012, London, UK, Clinical
Microbiology and Infection, 2012, 18, s3, 191. DOI: 10.1111/j.1469-0691.2012.03802.x.
Lorencova, A., Klanicova, B., Makovcova, J., Pavlik, I., Slany, M: The occurrence of mycobacteria in retailed
freshwater fish and fish products in the Czech Republic. Hygiena Alimentorum XXXIII, Strbske Pleso,
Slovakika, 9.-11.5.2012, ISBN 978-80-7148-063-1, 73-77.
Klanicova, B., Lorencova, A., Makovcova, J., Vlkova, H., Pavlik, I., Slany, M.: Survival of three Mycobacterium
avium subsp. hominissuis isolates in fish products detected by culture and real-time PCR after
smoking and frying. In: Annual Congress of the European Society of Mycobacteriology, 1.-4.7.2012,
Brasov, Romania, 82.
Kaevska, M., Sterba, J., Svobodova, J., Pavlik, I.: Mycobacterium neoaurum and M. avium subsp. avium
detection in immunocompromised patient and in his environment. In: Annual Congress of the
European Society of Mycobacteriology, 1.-4.7.2012, Brasov, Romania, 88.
Lorencova, A., Slana, I., Vasickova, P., Pavlik, I.: Tracing for hepatitis E virus and Mycobacterium avium
subspecies in raw meat products and usage of porcine adenovirus as indicator of faecal
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
contamination. In: 3rd Food and Environmental Virology Conference, Lisabon, Portugal, 7.10.10.2012, 57-59.
c)
National conferences
Kaevská, M., Štěrba, J., Pavlík, I.: Směsná infekce způsobená Mycobacterium avium subsp. avium a M.
neoaurum u imunosuprimovaného pacienta a možné zdroje infekce v jeho prostředí. In: Slezské dny
preventivní medicíny, Karviná, 7.-9.3.2012, ISBN 978-80-7329-312-3, CD, 1 s.
Klanicová, B., Seďa, J., Slaná, I., Kantorová, K., Slaný, M., Pavlík, I.: Sledování výskytu zástupců Mycobacterium
avium v sedimentech údolních nádrží, v úpravnách vod a v domácnostech v ČR. In: Slezské dny
preventivní medicíny, Karviná, 7.-9.3.2012, ISBN 978-80-7329-312-3, CD, 1 s.
Klanicová, B., Seďa, J., Slaná, I., Kantorová, K., Slaný, M., Pavlík, I.: Detekce druhu Mycobacterium avium
v sedimentech nádrží s pitnou vodou, v úpravnách vod a v domácnostech v ČR. XIV. Konference
mladých vědeckých pracovníků, Veterinární a farmaceutická univerzita, Brno, 29.5.2012, ISBN 97880-7305-612-4, 120-122.
Kaevska, M., Lvončik, S., Pavlík, I., Králík, P.: Kontaminace potravin rostlinného původu: je možná penetrace
mykobakterií kořenovým systémem do listů rostlin? In: XX. Moravsko - Slovenské mikrobiologické
dny, ISSN:1338-645X ISSN:1335-8219, Mikulov, 5.-7.9.2012, 31.
Lorencová, A., Klanicová, B., Makovcová, J., Pavlík, I., Slaný, M.: Detekce potenciálně patogenních
mykobakterií ve sladkovodních rybách a v rybích výrobcích. XX. Moravsko - Slovenské
mikrobiologické dny, ISSN:1338-645X ISSN:1335-8219, Mikulov, 5.-7.9.2012, 32.
Morávková, M., Svobodová, J., Kaevska, M., Slaný, M., Pavlík, I.: Infekce způsobené podmíněně patogenními
mykobakteriemi a došetřování jejich možných zdrojů z prostředí a z potravin. In: XX. Moravsko Slovenské mikrobiologické dny, ISSN:1338-645X ISSN:1335-8219, Mikulov, 5.-7.9.2012, 27.
Lorencová, A., Slaná, I., Vašíčková, P., Pavlik, I.: Sledování viru hepatitidy E a potenciálně patogenních
zástupců Mycobacterium avium subsp. v syrových masných produktech a užití porcinního adenoviru
k indikaci fekální kontaminace. In: 25. Pečenkovy dny, ISBN 978-80-904667, Harrachov, 18.20.9.2012, 102.
Annex to
d) Other: .......................................................................................
internet
Knowledge dissemination is an important activity of the OIE Reference Laboratories for Avian Tuberculosis,
and for paratuberculosis, supported by the Veterinary Research Institute, Brno, Czech Republic and
Biomedical Technology, Epidemiology and Food Safety Global Network. Databases on paratuberculosis and
Crohn’s disease digest were updated in 2012 with a total of 374 new scientific articles (236 and 138,
respectively), published in peer reviewed journals and indexed in the Web of Science® (Thomson Reuters)
database. Members of the Biomedical Technology, Epidemiology and Food Safety Global Network were
informed about new publications by e-mails in weekly intervals. Both databases are available at the web page
of the OIE Reference Laboratory for Paratuberculosis, hosted by the Veterinary Research Institute, Brno.
Network members from 25 countries (Argentina, Australia, Bangladesh, Canada, Chile, Czech Republic,
Denmark, Egypt, France, Germany, India, Ireland, Italy, Nigeria, Portugal, Republic of Korea, Saudi Arabia,
Slovakia, Slovenia, Spain, Switzerland, The Netherlands, Turkey, UK and USA) requested papers from those on
offer. In total, 60 new items of information on mycobacteria were distributed by e-mail to network members
as the Centaur Global Network Information and were simultaneously archived at the network web page. This
open access system means that all information is available not only for the Centaur Global Network
members. Everybody can register as a person, interested in any or all of the 27 fields of interest, one of which
is (08) Mycobacterial diseases. In total, 257 e-mail notifications were dispatched in 2012, mostly to
distribution lists (27) Scientific information, (23) Education, (05) Zoonoses, (04) Food borne diseases and (08)
6
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Tuberculosis » – « Ivo Pavlik » – « Czech Rebublic »
Mycobacterial diseases. The distance learning course on Good Research Practice was run also in 2012 and
the course assigned GRP 2013 has been opened in October 2012 and has to be finished by 31 March 2013.
The running course has registered 43 participants from 13 countries (Bangladesh, Belarus, Canada, Croatia,
Czech Republic, Ethiopia, India, Iran, Nigeria, Poland, Serbia, Slovakia, Yemen).
e)
f)
g)
h)
i)
j)
k)
l)
m)
n)
o)
p)
q)
r)
s)
t)
u)
v)
w)
URL addresses of hyperlinks:
OIE Reference Laboratories for Avian Tuberculosis
http://www.vri.cz/en/departments/laboratory-oie_reference_laboratory_for_avian_tuberculosis
Veterinary Research Institute, Brno
http://www.vri.cz
Biomedical Technology, Epidemiology and Food Safety Global Network (CENTAUR)
http://centaur.vri.cz
registration on line
http://centaur.vri.cz/?page=registration
Database on paratuberculosis
http://www.vri.cz/en/publications/paratuberculosis
Database on Crohn’s disease digest
http://www.vri.cz/en/publications/cd_and_map
OIE Reference Laboratory for Paratuberculosis,
http://www.vri.cz/en/departments/laboratory-oie_reference_laboratory_for_paratuberculosis
Distance learning course on Good Research Practice
http://centaur.vri.cz/?page=grp_e-course
GRP 2011 Report
http://centaur.vri.cz/docs/GRP2011/BULLETIN.pdf
Centaur Global Network Information
http://centaur.vri.cz/?page=cgn_information
Annual reports of OIE Reference Laboratories, 2012
7