Activities in 2009 Avian tuberculosis Professor Ivo Pavlik Veterinary Research Institute Hudcova 70, 621 00 Brno, Czech Republic Tel.: (+420-5) 3333.1601, Fax: (+420-5) 3333.1229 e-mail address: pavlik@vri.cz http://www.vri.cz/en/departments/food-and-feed-safety/oie-reference-laboratory-for-avian-tuberculosis/ Summary of general activities related to the disease 1. Test(s) in use/or available for the specified disease at your laboratory Test (Total) For Specificity ELISA Antibodies Sonicated antigen of M. avium subsp. avium (serotype 2) 40 pigs ELISA Antibodies Sonicated antigen of M. avium subsp. hominissuis (serotype 8) 40 pigs Rapid agglutination (202) Antibodies Corpuscular antigen of M. avium subsp. avium (serotype 2) 165 pigs, 2 cattle, 1 dog, 1 human, 21 horses, 12 ibises Rapid agglutination (202) Antibodies Corpuscular antigen of M. avium subsp. hominissuis (serotype 8) 165 pigs, 2 cattle, 1 dog, 1 human, 21 horses, 12 ibises Rapid agglutination (202) Antibodies Corpuscular antigen of M. intracellulare (serotype 19) 165 pigs, 2 cattle, 1 dog, 1 human, 21 horses, 12 ibises MAPIA (179) Antibodies Sonicated antigen M. avium subsp. avium (serotype 2) 53 wild ruminants, 26 cattle, 28 horses, 23 pigs, 49 animals from ZOO MAPIA (179) Antibodies Sonicated antigen M. avium subsp. hominissuis (serotype 8) 53 wild ruminants, 26 cattle, 28 horses, 23 pigs, 49 animals from ZOO MAPIA (179) Antibodies Corpuscular antigen of M. intracellulare (serotype 19) 53 wild ruminants, 26 cattle, 28 horses, 23 pigs, 49 animals from ZOO Culture examination (2308) Mycobacteria Conventional culture technique (media without Mycobactin J) 571 fish tissues, 326 pig tissues, 342 bird tissues, 6 human tissues, 95 other mammals tissues, 247 water environment, 413 pigs environment, 269 pigs faeces, 39 faeces and urine from other animals PCR Quadruplex (148) Isolates Quadruplex system for M. avium species differentiation1 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009 Total 24 M. a. avium 9 M. a. hominissuis 25 M. a. paratuberculosis 90 laboratory isolates 1 Avian tuberculosis Test (Total) For Specificity Total 16S rRNA PCR (230) Isolates 16S rRNA PCR system for Mycobacterium sp. and M. avium complex members identification1 IS1245 RFLP (22) Isolates Standardized method2 M. a. hominissuis IS901 RFLP (58) Isolates Standardized method3 M. a. avium 16S rDNA sequencing (101) Isolates Differentiation of Mycobacterium sp. Harmsen et al.5 and Telenti et al.6 Quantitative Real Time PCR (94) M. a. avium and M. a. hominissuis IS1245 and IS9017 112 M. avium sp. 1 M. intracellulare 107 other atypical mycobacteria4 4 M. arupense 1 M. arosiense 1 M. bohemicum 1 M. chimaera 2 M. chitae 7 M. fortuitum 1 M. engbaekii 7 M. gordonae 3 M. chelonae/abscessus 35 M. intracellulare 3 M. insubricum 4 M. kansasii 3 M. kumamotonense 1 M. mantenii 3 M. marinum 1 M. moriokaense 1 M. nonchromogenicum 1 M. simiae 1 M. szulgai 10 M. peregrinum/septicum 1 M. terrae 2 M. terrae complex 1 M. triviale 2 M. tuberculosis complex 1 M. xenopi 4 M. sp. 37 tissues 6 faeces 2 blood 43 environment 1Home made technique for the detection and differentiation of members of the M. avium species: Moravkova, M., Hlozek, P., Beran, V., Pavlik, I., Preziuso, S., Cuteri, V., Bartos, M.: Strategy for the detection and differentiation of Mycobacterium avium species in isolates and heavily infected tissues. Research in Veterinary Science, 2008, 85 (2), 257-264. 2Van Soolingen, D., Bauer, J., Leăo, S., Pavlik, I., Vincent, V., Rastogi, N., Gori, A., Bodmer, T., Garzelli, C., Garcia, M. J.: IS1245 Restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization. Journal of Clinical Microbiology, 1998, 36 (10), 3051-3054. 3Dvorska, L., Bull, T. J., Bartos, M., Matlova, L., Svastova, P., Weston, R. T., Kintr, J., Parmova, I., Van Soolingen, D., Pavlik, I.: A standardised restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS901 with virulence for birds. Journal of Microbiological Methods, 2003, 55 (1), 11-27. 4 Non-identified mycobacterial species other than members of M. avium species, M. avium complex and M. tuberculosis complex. 5Harmsen, D., Dostal, S., Roth, A., Niemann, S., Rothganger, J., Sammeth, M., Albert, J., Frosch, M., Richter, E., 11-11-2003. RIDOM: comprehensive and public sequence database for identification of Mycobacterium species. BMC Infection Diseases, 2003, 3, 26. 6Telenti, A., Marchesi, F., Balz, M., Bally, F., Böttger, E.C. and Bodmer, T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. Journal of Clinical Microbiology, 1993, 31 (2), 175-178. 7Specific fragments for M. avium subsp. avium and M. avium subsp. hominissuis ; home made technique, Slana, I., Kaevska, M., Kralik, P., Horvathova, A., Pavlik, I.: Distribution of Mycobacterium avium subsp. avium and M. a. hominissuis in artificially infected pigs studied by culture and IS901 and IS1245 quantitative Real Time PCR. International Journal of Food Microbiology, submitted. 2 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009 Avian tuberculosis 2. Production and distribution of diagnostic reagents None Activities specifically related to the mandate of OIE Reference Laboratories 3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines None 4. Preparation and supply of international reference standards for diagnostic tests or vaccines None 5. Research and development of new procedures for diagnosis and control Liquid culture examination of causal agent of avian tuberculosis: Shitaye, J.E., Beran, V., Svobodova, J., Moravkova, M., Babak, V., Pavlik, I.: Comparison of the conventional culture, the Manual Fluorescent MGIT System and the Automated Fluorescent MGIT 960 Culture System for the detection of Mycobacterium avium subsp. avium in tissues of naturally infected hens. Folia Microbiologica, 2009, 54 (2), 137-141. 6. Collection, analysis and dissemination of epizootiological data relevant to international disease control None 7. Provision of consultant expertise to OIE or to OIE Members None 8. Provision of scientific and technical training to personnel from other OIE Members Under the terms of OIE Reference laboratory three laboratory trainings are provided: Cultivation, microscopy and identification methods for the detection of mycobacteria (5 days training) http://www.vri.cz/userfiles/file/hide/06-CLT-1.pdf The quantification of Mycobacterium avium subsp. avium in milk using real-time qPCR (5 days) http://www.vri.cz/userfiles/file/hide/06-qPCR-1.pdf The differentiation of Mycobacterium avium subsp. avium and using RFLP analysis (7 days) http://www.vri.cz/userfiles/file/hide/06-RFLP-1.pdf Marina Mikulic, DVM.; Croatian Veterinary Institute Zagreb, Croatia; training in Quantification of M. avium species members using real-time qPCR, January 2009. Ing. Ivona Mateska, St. Cyril and Metodius University of Skopje, Macedonia, 13.10.2008 - 12.4.2009. The detection of mycobacteria after applying stress factors. Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009 3 Avian tuberculosis 9. Provision of diagnostic testing facilities to other OIE Members None 10. Organisation of international scientific meetings on behalf of OIE or other international bodies None 11. Participation in international scientific collaborative studies National Grants Animal disease research, prevention and food chain protection, part: Mycobacterial infections of animals. Institutional Research Plan, 1.1.2009-31.12.2013. Ministry of Agriculture, the Czech Republic, No.: MZE 0002716202, Project leader: Prof. MVDr. Jiri Rubes, CSc., VRI Brno, Czech Republic Efficiency of alternative methods for prevention of enteric diseases in pigs and determination of risks from an aspect of food safety. National Agency for Agricultural Research (NAZV), No. QH71054, 1.1.200731.12.20011, Project leader: Prof. MVDr. Ivo Pavlik, CSc., VRI Brno, Czech Republic. Mycobacterial infections in freshwater fish and their impact for livestock and humans. National Agency for Agricultural Research (NAZV), No. QH91240, 1.1.2009-31.12.20011, Project leader: Prof. MVDr. Ivo Pavlik, CSc., VRI Brno, Czech Republic. European Union Grants Veterinary Network of Laboratories Researching into Improved Diagnosis and Epidemiology of Mycobacterial Diseases. Project Acronym: VENOMYC, No. SSPE-CT-2004-501903, EU, Brussels, 1.10.200331.8.2009, Co-ordinator: Prof. Lucas Dominguez, Dpt. Patologia Animal I, Sanidad Animal Facultad de Veterinaria U.C.M., Madrid, Spain. Center for Advanced Microbiology & Immunology Research in Veterinary Medicine. Acronym: AdmireVet. Registration No. CZ.1.05/2.1.00/01.0006. Regional R&D Centre. Ministry of Education, Youth and sports of the Czech Republic. 1.12.2009-31.12.2013. Co-ordinator: Prof. MVDr. Ivo Pavlík, CSc. 12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) Presentations at international conferences and meetings Pavlik, I., Mrlik, V., Slana, I., Svobodova, J., Kralik, P., Skoric, M., Fictum, P., Moravkova, M., Krizova, K.: Human tuberculosis in domestic animals (esp. dogs and pigs) and unrecognised threat of exposed environment. In: Proceedings of the Fifth International M. bovis Conference, 25-28 August 2009, Wellington, New Zealand, 42. Krizova, K., Mrlik, V., Boisselet, T., Beran, V., Kriz, P., Babak, V., Vondruskova, H., Slamova, R., Kaevska, M., Slana, I., Pavlik, I.: Safety of home-made meat products regarding survival of Mycobacterium avium subsp. hominissuis. Food Safety Conference, Nitra, Slovakia, 3-4.12.2009, 8. Scientific publications in peer-reviewed journals Trckova, M., Vondruskova, H., Zraly, Z., Alexa, P., Hamrik, J., Kummer, V., Maskova, J., Mrlik, V., Krizova, K., Slana, I., Leva, L., Pavlik, I.: The effect of kaolin feeding on efficiency, health status and course of diarrhoeal infections caused by enterotoxigenic Escherichia coli strains in weaned piglets. Veterinarni Medicina, 2009, 54 (2), 47-63. http://www.vri.cz/docs/vetmed/54-2-47.pdf Shitaye, J.E., Grymova, V., Grym, M., Halouzka, R., Horvathova, A., Moravkova, M., Beran, V., Svobodova, J., Dvorska-Bartosova, L., Pavlik, I.: Mycobacterium avium subsp. hominissuis infection in a pet parrot. Emerging Infectious Diseases, 2009, 15 (4), 617-619. 4 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009 Avian tuberculosis Shitaye, J.E., Beran, V., Svobodova, J., Moravkova, M., Babak, V., Pavlik, I.: Comparison of the conventional culture, the Manual Fluorescent MGIT System and the Automated Fluorescent MGIT 960 Culture System for the detection of Mycobacterium avium subsp. avium in tissues of naturally infected hens. Folia Microbiologica, 2009, 54 (2), 137-141. Pate, M., Moravkova, M., Krt, B., Pavlik, I., Ocepek, M.: Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP. Veterinarni Medicina, 2009, 54 (6), 270-279. http://www.vri.cz/docs/vetmed/54-6-270.pdf Shitaye, J.E., Horvathova, A., Bartosova, L., Moravkova, M., Kaevska, M., Donnelly, N., Pavlik, I.: Distribution of non-tuberculosis mycobacteria in the environmental samples from slaughterhouse and in raw and processed meats. Czech Journal of Food Sciences, 2009, 27 (3), 194-202. Kazda, J., Pavlik, I., Falkinham, J., Hruska, K. (eds.): The ecology of mycobacteria: impact on animal’s and human’s health. Second edition, Springer, 2009, ISBN 978-1-4020-9412-5, 520 pp. Other communications Knowledge dissemination is the integral part of activity of OIE Reference Laboratories for Paratuberculosis and Avian Tuberculosis. The databases have been updated weekly using the records, indexed by the Web of Science database (Thomson Reuters®). More than 2000 subscribers for (08) Mycobacterial diseases and (27) Scientific information (Veterinary Biotechnology, Epidemiology and Food Safety Network http://centaur.vri.cz) received by e-mail the annotations of recently published papers. In 2009, the database “Publications on Paratuberculosis” (http://www.vri.cz/en/ptb) has been updated by 233 papers published in 118 scientific journals. The database “Crohn’s Disease and Paratuberculosis Digest” (http://www.vri.cz/en/cd-ptb-digest) has been updated by 129 records of articles, published in 93 journals. During the year 2009 a total 746 full papers (3-fold increase of interest in comparison with 2008) have been dispatched to applicants from 17 countries (Australia, Bulgaria, China, Czech Republic, Egypt, France, Italy, Ireland, India, Saudi Arabia, Slovakia, Slovenia Spain, Switzerland, The Netherlands, United Kingdom and USA). In addition to 32 e-mails with information on databases updates, 50 other information on EU documents, international meetings, interesting case reports, new books, distance learning course etc. have been distributed among people, interested in mycobacteria research and control. 13. Inscription of diagnostic kits on the OIE Register i) Did you participate in expert panels for the validation of candidate kits for inscription on the OIE Register? If yes, for which kits? None ii) Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for which kits? None _______________ Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009 5