Cell The cell is the basic structural, functional, and biological unit of all known living organisms. Cells are the smallest unit of life that can replicate independently, and are often called the "building blocks of life". The study of cells is called cell biology. Cells consist of cytoplasm enclosed within a membrane, which contains many biomolecules such as proteins and nucleic acids. Organisms can be classified as unicellular (consisting of a single cell; including bacteria) or multicellular (including plants and animals). While the number of cells in plants and animals varies from species to species, humans contain more than 10 trillion cells. Cell Theory All living things are made of cells Smallest living unit of structure and function of all organisms is the cell All cells arise from preexisting cells (this principle discarded the idea of spontaneous generation) Types of Cells 1- Prokaryotic Cells The simplest type of cells were the first type of cells that formed on Earth. All prokaryotic cells have a cell membrane surrounding the cell, cytoplasm where all of the metabolic processes happen, ribosomes that make proteins, and a circular DNA molecule called a nucleoid where the genetic information is held. The majority of prokaryotic cells also have a rigid cell wall that is used for protection. All prokaryotic organisms are unicellular, meaning entire organism is only one cell. Prokaryotic organisms are asexual, meaning they do not need a partner to reproduce. Most reproduce through a process called binary fission where basically the cell just splits in half after copying its DNA. This means that without mutations within the DNA, offspring are identical to their parent. Prokaryotic organisms include Archaea and Bacteria. 2- Eukaryotic Cells The other, much more complex, type of cell. Like prokaryotic cells, eukaryotic cells have cell membranes, cytoplasm, ribosomes, and DNA. There are many more organelles within eukaryotic cells. These include a nucleus to house the DNA, a nucleolus where ribosomes are made, rough University of Al-Qadisiyah College of Dentistry Department of Basic Science Practical Biology Lab (3) 1 endoplasmic reticulum for protein assembly, smooth endoplasmic reticulum for making lipids, Golgi apparatus for sorting and exporting proteins, mitochondria for creating energy, a cytoskeleton for structure and transporting information, and vesicles to move proteins around the cell. Cell walls can also be found surrounding some types of eukarotyic cells. Most eukaryotic organisms are multicellular. This allows the eukaryotic cells within the organism to become specialized. There are a few unicellular eukaryotes as well. Eukaryotes may use either asexual or sexual reproduction depending on the organism's complexity. Eukaryotic organisms include all animals, plants, protists, and fungi. Shapes of cell 1. Squamous: flat, thin, scale-like cells. 2. Spheroid: round to oval cells. 3. Polygonal: irregular angular shapes, with more than 4 sides. 4. Discoid: disc shaped. 5. Cuboidal: squarish. 6. Fusiform (spindle shaped): thick middle with tapered ends. 7. Columnar: taller than wide. 8. Fibrous: long, slender. 9. Stellate: star shape. University of Al-Qadisiyah College of Dentistry Department of Basic Science Practical Biology Lab (3) 2 Components of cell Organelles are tiny structures within cells that perform specialized tasks and are often surrounded by their own membrane. These organelles perform the functions necessary to keep the cell alive. While there are many different types of cells making up an organism, most of these cells contain the same set of organelles. 1. Cytoplasm Viscous fluid containing organelles Components of cytoplasm – Interconnected filaments & fibers – Fluid = cytosol – Organelles (not nucleus) – Storage substances 2. Cell membrane Outer membrane of cell that controls movement in and out of the cell Double layer of phospholipids & proteins 3. Cell wall Most commonly found in plant cells & bacteria Supports & protects cells Plants – mostly cellulose Fungi – contain chitin 4. Nucleus Directs cell activities Separated from cytoplasm by nuclear membrane Contains genetic material – DNA 5. Nuclear membrane Surrounds nucleus Made of two layers Openings allow material to enter and leave nucleus 6. Chromosomes In nucleus Made of DNA Contain instructions for traits & characteristics 7. Nucleolus Inside nucleus Most cells have 2 or more Directs synthesis of RNA Forms ribosomes 8. Ribosomes Each cell contains thousands University of Al-Qadisiyah College of Dentistry Department of Basic Science Practical Biology Lab (3) 3 Small dark granules of protein and RNA free in cytosol or on surface of rough ER Interpret the genetic code and synthesize polypeptides (Make proteins). 9. Endoplasmic reticulum Helps move substances within cells Network of interconnected membranes Two types: – Rough endoplasmic reticulum – Smooth endoplasmic reticulum 10.Golgi bodies Synthesizes CHO’s, processes proteins from RER and packages them into golgi vesicles Golgi vesicles – irregular sacs near golgi complex that bud off cisternae – some become lysosomes, some fuse with plasma membrane and some become secretory vesicles. Secretory vesicles – Store a cell product for later release. 11.Vacuoles Membrane bound storage sacs More common in plants than animals Contents: – Water – Food – Wastes 12.Vesicles Storage organs found in animal cells. These tend to be much smaller then plant vacuoles. 13.Mitochondria Produces energy through chemical reactions – breaking down fats & carbohydrates Controls level of water and other materials in cell Recycles and decomposes proteins, fats, and carbohydrates 14.Lysosomes Package of enzymes in a single unit membrane, variable in shape Functions: – intracellular digestion - hydrolyze proteins, nucleic acids, complex carbohydrates, phospholipids and other substrates – autophagy - the digestion of worn out organelles and mitochondrion – autolysis - programmed cell death University of Al-Qadisiyah College of Dentistry Department of Basic Science Practical Biology Lab (3) 4 – glucose mobilization - lysosomes in liver cells break down glycogen 15.Chloroplasts Usually found in plant cells. Contains green chlorophyll. Where photosynthesis takes place. University of Al-Qadisiyah College of Dentistry Department of Basic Science Practical Biology Lab (3) 5