On-line Electrophoresis

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On-line DNA Extraction
Go to this website and answer the following questions as you do the activity.
http://learn.genetics.utah.edu/content/labs/extraction/
1. Why do we need human DNA? The DNA extraction process is a precursor to
many diagnostic processes. List, research and describe the three purposes.
2. What are the 4 basic steps of the DNA extraction process? Explain the process
and purpose of each step.
3. What can DNA be extracted from?
On-line Electrophoresis
Go to the following website and answer the following questions as you do the activity
http://learn.genetics.utah.edu/content/labs/gel/

List and describe each step of the gel electrophoresis process.
(make sure you include in your discussion for step 1 the purpose of the buffer, in
step 3 the purpose of the DNA size standard, in step 3 how the DNA pieces move,
in step 4 an explanation of how ethidium bromide works and why it should be
handled with gloves, in step 5 include a sketch of the gel with the DNA bands and
DNA size standard(ladder)
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