Directions for one-way ANOVA in Microsoft Excel 2007
In order to complete Hypothesis Testing Experiment #4 (HTE #4), you will need to compute descriptive
statistics, a one-way ANOVA, and create graphs. The directions for these activities are contained in your
lab manual, but they were written for Microsoft Excel 2003 and earlier versions. The computers in HOH
315 have been upgraded to Excel 2007 and the appearance of Excel has changed greatly in the latest
release. The directions for computing descriptive statistics and creating graphs were discussed in the
previous handout. These directions will describe how to:
1. Check to make sure that the Data Analysis tab is visible
2. Perform a one-way ANOVA
Before we begin, you will need to open Microsoft Excel 2007.
Part 1: Making the Data Analysis tab visible
1. Click on the Data tab and see if there is an Analysis box with the Data Analysis contained
within it. If it is present, your screen will look like this:
2. If Data Analysis is not present, you will need to load the Analysis Toolpack. The directions or
loading the Analysis Toolpack are as follows.
a. Click the Microsoft Office Button
(top left of screen), and then click Excel
Options.
b. Click Add-ins, and then in the Manage box, select Excel Add-ins.
c. Click Go.
d. In the Add-Ins available box, select the Analysis ToolPak check box, and then click
OK.
e. Data Analysis should now be present under the Data tab.
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Part 2: Perform a one-way ANOVA
If you are comparing only two groups at a time, a t-test should be used. If you are comparing the means
of three or more groups then an ANOVA (ANalysis Of VAriance) is used. Here’s how to run a one-way
ANOVA:
1. Input your data into Microsoft Excel so that your measurements are in three columns (one
for each water temperature). Your screen will look like this:
2. Save your work
3. Click on the Data tab, then click Data Analysis. A new window will appear that looks like this:
4. Select ANOVA: Single Factor and click OK.
5. Define your input range to include all the numbers in all three columns (e.g. all “wiggles”
that were recorded for all temperatures)
6. Define your output range so that the output doesn’t overwrite any of your data. Your screen
should now look like this:
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7. Click OK
8. Click the Home tab, then find the Cells box and click on Format. Select AutoFit Column
Width.
9. Your output should look like this:
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10. You will then use this output to determine whether there was a significant difference in the
means between your three groups.
a. Remember, if p < 0.05 there is a significant difference. If p > 0.05 there is not a
significant difference.
b. The output from this experiment could be written up:
i. As Figure 1 shows, there is a significant difference in the number of
Turbatrix undulations per thirty seconds between the 1 °C, the 19 °C and the
38 °C groups (F2,36 = 96.656, p = 3 *10-15).
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