The use of polymerase chain reaction (PCR)

International Journal of Advanced Scientific and Technical Research Issue 3 volume 5,Sep.-Oct. 2013
Available online on ISSN 2249-9954
R S. Publication, [email protected] Page 11
The use of polymerase chain reaction (PCR) in confirmation of
the microbiologicaly isolated Staphylococcus aureusfrom
wound and urinary tract infections Yousif Fadlalla Hamedelnil 1 Maysaa
Mahadi Jumaa Adam 2
1- Associate professor : Medical Laboratory Technology Sudan University of science and
2- Medical Laboratory Department Khartoum
___ Abstract This study was aimed to investigate the presence of Staphylococcus aureus
among patients with wound and urinary tract infections and to subject the isolated
S.aureus to Polymerase Chain Reaction (PCR) in Khartoum state. The study was
performed during the period from March to June 2011. It included 100 samples from
wound and urine infection from patients with different sex and age .The samples were
cultured on Blood agar ,Macckoncy’s and CLED agar incubated aerobically at 37 C.
Gram stain was done for bacterial isolates. Biochemical tests were used to identify
isolated bacteria. Polymerase Chain Reaction amplification target gene arcC (crabmeat
Kainase ) was done for isolated S.aureus .The study revealed that S.aureus was isolated
from samples was 23.8% and Methicillin-resistant Staphylococcus aureus(MRSA)
percentage was 80%. DNA extraction By Phenol chloroform isomayle alcohol were done
for 10 isolates ,PCR showed 2 isolates (20%) were arcC primer recognized and 8 isolates
(80%) were not arcC primer. This study showed that the identification of Staphylococcus
aureus by polymerase chain reaction by amplification arcC gene is not desirable due to
the limited of this gene in the strains of S.aureus.