Important notes: Do NOT write outside the grey boxes. Any text or images outside the boxes will be deleted. Do NOT alter the structure of this form. Simply enter your information into the boxes. The form will be automatically processed – if you alter its structure your submission will not be processed correctly. Do not include keywords – you can add them when you submit the abstract online. Title: Diversity Of Ovine Associated Staphylococcus aureus Authors & affiliations: E. Smith1*, P. Needs1, L. Green1 School of Life Sciences, University of Warwick, England edward.smith@warwick.ac.uk 1 Abstract: (Your abstract must use Normal style and must fit in this box. Your abstract should be no longer than 300 words. The box will ‘expand’ over 2 pages as you add text/diagrams into it.) Mastitis, inflammation of the mammary gland, is a common disease of dairy and meat sheep. The mammary gland of meat ewes is exposed to many pathogens over a lifetime and many ewes have subclinical carriage of bacteria. On occasion clinical mastitis occurs, farmers report the incidence of clinical mastitis to be 0 – 5%. Both clinical and subclinical infections reduce farm profitability because reduced milk production leads to reduced weight gain in suckling lambs. Clinical mastitis causes disease and death in ewes, and is treated with antibiotic therapy, but whilst it might prevent death it is rarely successful in returning the affected gland to milk production. The bacterial species isolated from intra-mammary infections (IMI) in sheep are similar to those isolated from other ruminants, and include Escherichia coli, Staphylococcus aureus, coagulase-negative staphylococci and streptococcal species. The aim of this project was to investigate the diversity of global ovine S. aureus using multilocus sequence typing (MLST) to characterise isolates from sheep and sheep products, and included isolates from the UK, Turkey, France, Norway and the USA. We detected novel sequences and sequence types. Ovine isolates are distinct from those of human and bovine origin. The majority of isolates belong to a single clonal complex (CC133) that has achieved widespread geographical dissemination, followed by clonal expansion of types unique to individual countries or regions. The detection of the majority of isolates within a single clonal complex may indicate a level of hostspecificity, as has been described in studies of bovine S. aureus. However S. aureus CC133 is prevalent in domestic pets and exotic species, suggesting CC133 is capable of colonising and infecting a variety of animals. This apparent lack of diversity between host species may be biologically relevant and might indicate a historic lack of selection pressure (e.g. antibiotic use) on the pathogen.