DANAGENE BLOOD DNA Kit
Allows genomic DNA high quality purification from blood
or mammalian bone marrow.
Procedure
This kit is based on a simple and fast lysis and protein
elimination process without using toxic reagents, organic
solvents or enzymatic treatments.
The kit can be used for DNA extraction from fresh o frozen
blood collected in tubes containing citrate, heparine or EDTA.
For a high yield, tubes containing EDTA are recommended.
Features:

Reproducible, fast and non-expensive
method.

This method can be scaled allowing to process
large amounts of samples simultaneously.

Safe method, as it removes completely the
need of using toxic reagents.

Typical yield of 35 g/ml of blood with an
A260/280 of 1.7-1.9.

It is completed in 45-60 minutes.
RBC
Lysis
Cell lysis
M
Protein DNA
precipitation
1
2
DNA Wash DNA
hydration
3
4
Genomic DNA analysis in agarose gel.
Purified from whole blood using the
DANAGENE BLOOD DNA Kit
Applications: DNA purified using this kit is highly
stable and suited for use in a wide range of
applications such as:





M
1
2
3
4
DNA archiving.
PCR and quantitative real-time PCR.
SNP analysis.
Southern Blotting.
Sequencing.
DANAGENE BLOOD DNA Kit
Ref.0601 for 100 ml of blood.
Ref.0602 for 200 ml of blood
PCR amplification was performed on
DNA isolated from blood.
600 bp amplicon was obtained.