DANAGENE Plant DNA Kit
This kit provides a method for an efficient and
fast genomic DNA extraction from tissues
of plants and fungi.
It is known that plants contain quantities of
d i ffe r e n t s u b s t a n c e s ( p o l ys s a c c h a r i d e s ,
polyphenols, etc) and that plants with the same o r
related genus can p r esent eno r mo us
variabilities in their biochemical composition,
fo r such reaso n, it b eco me s d iffic ult to
standardise on a single DNA extraction method
for all plants.
To solve this problem and to be able to cover the
biggest number of plants, it uses a PVP solution
that can bind the polyssacharides and
polyphenols that are released by the cell lysis
and has the capability of forming complexes with
the nucleic acids to degrade them or to
precipitate with them.
Features:

Reproducible, fast and non-expensive
method.

Convenient and scalable purification
procedure.

Safe method, as it removes completely the
need of using toxic reagents.

It is completed in 45-60 minutes.

It contains a PVP solution that allows
working with plants with a high
content polyssacharides and
polyphenols compounds.
Applications: DNA purified using
DANAGENE Plant DNA Kit is highly
stable and suited for use in a wide range
of applications such as:
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Procedure:The
process includes a sample
homogenization in an extraction Buffer and the
PVP solution. The lysis is completed with the
incubation in a Lysis Buffer and RNase at 37°C for
30 minutes. Cell proteins and cell debris are removed
by a protein removing Buffer that allows to leave the
genomic DNA in solution. Finally, the genomic
D N A i s i s o l a t e d b y a p re c ip i t a t io n wi t h
isopropanol.
Cell lysis +
Protein
DNA
PVP solution+
Precipitation
RNase
M 1
2
3
4
5 6
7 8
DNA Wash DNA
Hydration
9 10 11 12 M
Genomic DNA from different plants.
Genomic DNA was isolated using DANAGENE Plant
DNA Kit from 20-40 mg of the following leaves or trees
M. Molecular weight marker
1 y 2. Corn
3 y 4. Orange tree
5 y 6. Olive tree
7 y 8. Tomato
9 y 10. Lemon
11 y 12. Eucaliptus
DNA archiving.
PCR and quantitative real-time PCR.
SNP analysis.
Southern Blotting.
Sequencing.
DANAGENE Plant DNA Kit
Ref.0604.1 Includes RNase.
For 50 extractions from 20-40 mg of plants or
fungi tissues.
DANAGENE Plant DNA Kit
Ref.0604.2. Includes RNase.
For 200 extractions from 20-40 mg of plants
or fungi tissues.