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Supplementary Material
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Analysis of cbbL, nifH and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals
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a large diversity of auto- and phototrophic bacteria
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Guillaume Tahon, Bjorn Tytgat, Pieter Stragier, Anne Willems*
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*Correspondence: Anne Willems, Laboratory of Microbiology, Department of Biochemistry and
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Microbiology, Ghent University, Ghent, Belgium, Anne.Willems@UGent.be
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Fig. S1 Comparison of genetic fingerprints (DGGE) of two terrestrial samples collected near the
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Princess Elisabeth Station, by use of five different DNA extraction methods. Partial 16S (V1-V3
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region) was amplified using primers V3-357F_GC and V3-518R (Temmerman et al., 2003). Partial
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pufM gene fragments were amplified using primers pufM557F (Achenbach et al., 2001) and
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GC_WAWR (Yutin et al., 2008). Gene fragments retrieved were visualized using DGGE. Lanes
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designated as 1 and 2 correspond with the two terrestrial samples tested. Different DNA extraction
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methods used are labeled as PL (PowerLyzer® PowerSoil® DNA Isolation Kit), C (CTAB), M
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(Muyzer), F (FastDNA™ SPIN Kit for Soil) and P (Pitcher). In total, seven different DNA extraction
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methods were tested: CTAB, Pitcher, Muyzer, PowerLyzer® PowerSoil® DNA Isolation Kit,
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FastDNA™ SPIN Kit for Soil, PowerSoil® DNA Isolation Kit and UltraClean Soil DNA Isolation
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Kit. For all methods, protocols were tested with and without modifications (e.g. the influence of bead
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bating or an extra lysis step), to optimize DNA yield and diversity. Partial pufM and 16S rRNA were
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amplified as described above and visualized using DGGE. The resulting best five DNA extraction
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methods were compared in the figure
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