Supporting Information for publication
Middle-down hybrid chromatography/tandem mass spectrometry workflow for characterization of
combinatorial post-translational modifications in histones
Simone Sidoli§, Veit Schwämmle§, Chrystian Ruminowicz, Thomas A. Hansen§, Xudong Wu¶, Kristian Helin¶ and Ole
N. Jensen§‡
§ Centre for Epigenetics, Department of Biochemistry and Molecular Biology, University of Southern Denmark,
Campusvej 55, DK-5230 Odense M, Denmark
¶ Centre for Epigenetics, Biotech Research and Innovation Centre, University of Copenhagen, DK-2200, Copenhagen,
Denmark
‡To whom correspondence should be addressed:
Prof. Ole N. Jensen, Department of Biochemistry and Molecular Biology, University of Southern Denmark,
Campusvej 55, 5230 Odense M, Denmark.
Tel: +45 6550-2368. Fax: +45 6550-2467. E-mail: jenseno@bmb.sdu.dk.
Index
1) Supporting information abstract (page 2)
2) Results: optimization of MS/MS detection without dynamic exclusion (page 2)
3) Results: Histone Coder principle of operation and performance
4) Results: isoScale principle of operation and performance (page 3)
5) Results: comparison of histone H3 marks quantified in crude histone mixture and purified fraction (page 4)
6) Results: comparison of H3K27K36 results with previous publication of Jung et al. (MCP, 2010) (page 4)
7) Results and discussion: middle-down analysis of histone isotypes H3.2/H3.1 and H3.3 (page 5)
8) References (page 5)
9) Supplementary figures and tables – captions (page 6)
10) Supplementary figures and tables (page 8)
1) Supporting information abstract
We provide here supporting data regarding the methodology we implemented for the middle-down proteomics
workflow. We demonstrate, among others, that (i) by not using dynamic exclusion (DE) in the MS/MS acquisition
method we obtained exhaustive identification of isobaric polypeptides, (ii) the deconvolution algorithm Xtract
performs better for our workflow than other widely used ones, (iii) isoScale provides robust quantification
independently of sample purity, (iv) our results are comparable with previous publication where bottom-up
proteomics and SILAC labeling was adopted.
2) Results: optimization of MS/MS detection without dynamic exclusion
We optimized the MS acquisition method to accurately map the PTMs along the peptide sequence and detect isobaric
modified peptides. Extensive peptide sequence coverage by MS/MS is especially required for histone tails, as they
have numerous potential modification sites; e.g. in histone H3, the PTMs ac and me3 potentially localize on 8 different
K, while me1 and me2 on 14 sites between K and R. As previously demonstrated, ETD is the most suitable
fragmentation technique for effective histone tail fragmentation in hybrid MS such as the LTQ-Orbitrap, due to its
efficiency for long and heavily charged peptides such as histone tails [1, 2] (Fig. S3). The acquisition method was
implemented to perform Data Dependent Acquisition (DDA), where the 6 most intense ions were selected at each MS
cycle for MS/MS fragmentation, without adopting dynamic exclusion (DE). DE is commonly enabled to avoid repetitive
selection of previously fragmented peptides, but it prevents the selection of isobaric peptides. We tested the
acquisition method by analyzing the purified fraction of histone H3 of wild type and Suz12 -/- ESCs. From this analysis
we identified 713 different histone H3 combinatorial marks (Table S3), which is currently an unmatched result in the
entire literature for a single protein. This result overcame more than 6-fold our previous report, where we identified
114 combinatorial marks in ESCs with the same sample preparation, but different LTQ-Orbitrap (XL vs Velos) [2]. The
2
713 differently modified peptides identified had only 37 different nominal masses (approximated without decimal
values), indicating that each precursor mass corresponded to almost 20 different PTMs patterns. Therefore, the use of
DE is not recommended to identify isobaric peptides.
3) Results: Histone Coder principle of operation and performance
The role of Histone Coder is to verify whether the PTM localization assigned by Mascot is supported by fragment ions
present in the MS/MS spectrum. To do so, the software counts the number of `site determining ions´ between the
assigned PTM localization and the two closest alternative sites. If ions are found, the Mascot assignment is considered
unambiguous and certain. Mascot can provide up to 10 possible hits for each MS/MS spectrum; Histone Coder
considers each of them separately and assigns site determining ions for all the PTM combinations provided by Mascot.
In some cases, the MS/MS spectrum contains site determining ions for more than one hit (Fig. S5); this leads to the
validation of multiple hits from the given spectrum. Spectra containing multiple hits validated by Histone Coder are
candidates for isoScale quantification (see section 4).
The principle of Histone Coder is similar to other proteomics tools such as PhosphoRS [3]. However, Histone Coder
includes unique features that make it currently the only available tool for middle-down proteomics analysis. For
example, Histone Coder offers a simple user interface, while other algorithms such as the mixed linear optimization
framework developed in DiMaggio, et al. [4] are currently not available. Also, Histone Coder copes with multiple
different PTMs residing within the same peptide sequence; other tools such as PhosphoRS [3], the A-score [5], the
PTM-score [6] and the Mascot Delta score [7] are effective, but only for single modifications (mostly phosphorylation).
The output of Histone Coder does not provide a probability score, but it includes all information about number and
type of site determining ions detected in the MS/MS spectrum. In the output table the column named `Site
determining ions´ lists the site determining ions for each assigned PTM (Fig. 2B and S10), while the column named
`Modification confidence´ lists the number of site determining ions, type (e.g. c32, z41) and how many potential
fragments could be detected. For instance, in figure S11A two over four ions were identified between R2 and K4 to
localize the me1, which are c2 and (z+1)48; this last one was not mapped by Mascot, due to higher stringency applied
during database searching to discriminate ac and me3. Eventually, a probability score can be calculated manually by
the user based on the provided information. However, we recommend the use of the site determining ions column to
filter for reliable hits; the probability score is calculated based on the number of potential fragment ions identified
between two aa residues, but the ETD fragment ions of a histone tail do not have equal probability to be generated.
Specifically, larger mass ions are less frequent than smaller ones, and c fragments are generally more frequent than
z+1 or z+2 ones (Fig. S4 and S11). Therefore, PTMs in the high mass region (e.g. H3K27ac or H3K36ac) would result as
generally less confident than PTMs in small mass region (e.g. H3K4ac or H3K9ac), even though they had sufficient
fragment ions to prove their localization as unambiguous.
3
4) Results: isoScale principle of operation and performance
isoScale was implemented to retrieve the data for peptide quantification directly from the Mascot output. The logo of
isoScale was designed to metaphorically represent the ability of the software in performing relative quantification of
isobaric peptides (Fig. S7A). To exploit the information of MS/MS spectra containing the fragments of multiple isobaric
peptides we made isoScale able to extract from the Mascot .csv result file(s) the fragment ion relative ratio (FIRR) of
two peptides differentially modified and fragmented in the same MS/MS spectrum. This file is the one we define as
Data file. The Result file is the output of Histone Coder, where the ambiguous PTM assignments were removed. We
recommend to not change the sorting of the table in the output of Histone Coder, as isoScale uses the two top-ranking
identifications for each query, and the raw output is already sorted from higher to lower Mascot score. As previously
demonstrated by Pesavento et al. [8] the intensity of the fragment ions can be adopted to estimate the relative
abundance of different isobaric co-fragmented species. To do so, isoScale extracts the unique ions for each of the two
identified peptides, where for `unique ions´ is intended all the fragment ions that univocally discriminate one peptide
from the other. As illustrated in Figure S7B, an MS/MS spectrum might contain multiple isobaric histone tails (in the
example, H3K9me3 and H3K4me1K9me2). The unique ions are the fragment ions included from K4 to A7, as they have
different masses in the two species (R8 would be also a unique ion, but it was not identified). They are used by the
searching engine to identify the two peptides. The FIRR is calculated by using the average FIRR of each individual
fragment type (e.g. K4/K4*, Q5/Q5*, T6/T6*…). isoScale extracts this information, and it divides the total ion intensity
of the MS/MS spectrum for such ratio, and it divides the intensity between the two species. To avoid coarse errors in
the calculation of such ratio we included the option for fragment mass tolerance, which we recommend as <0.05 Da
for high resolution data. In addition, we applied a filter for FIRR variability; once the software calculates the individual
rations of the various fragment types (e.g. K4/K4*, Q5/Q5*, T6/T6*…) it generates the average and it calculates the
coefficient of variation based on the individual FIRRs. In case such coefficient of variation exceeds 50% the ratio is
considered as null and the total ion intensity is completely assigned to the top probable identification. This limit was
integrated as Pesavento et al. proved that FIRR variability between individual fragment types is generally very small
(estimated to be around 5% for species in 1:1 ratio) [8]. Therefore, highly variable FIRRs indicates erroneous
calculation of the peptide relative abundance. Furthermore, isoScale is suitable for both CID and ETD spectra.
Once the total ion intensity is extracted for each identified and validated peptide the total abundance of a given
peptide is calculated by summing all total ion intensities obtained from all spectra where this peptide was identified
(Fig. S7C). FIRR was calculated and the total ion intensity of each spectrum was then divided between the two cofragmented peptides. Total peptide abundance was calculated in the same manner (Fig. S7D). This approach
overcomes the challenge of partially overlapping chromatographic profiles of two isobaric peptides; i.e. in spectra
where the two peptides overlap, the total ion intensity is the sum of both co-fragmented peptides, differently the
MS/MS spectrum total ion intensity corresponds to the fragmentation of a single peptide. In those cases where two
peptides partially overlap the total abundance was calculated from MS/MS spectra using the FIRR correction and also
from additional spectra where the peptide ion was isolated alone (Fig. S7E).
4
We assessed the performance of isoScale in the main text of the article (Fig. 3). In addition, we include here further
details regarding the comparison between isoScale and spectral counting quantification. By analysing the three
technical replicates of the purified histone H3 fraction from Suz12 -/- cells we quantified 357 combinatorial PTMs with
isoScale and 239 with spectral counting, considering peptides detected in at least one of the three replicates. Despite
the larger number of combinatorial marks identified both quantification strategies achieved a similar percentage of
missing values, defining as missing value a combinatorial histone code not identified in one of the three technical
replicates (1 missing value) or two of them (2 missing values). For isoScale 43.3% was the average missing values
percentage in each of the three replicates, while for spectral counting it was 44.5%. This indicated that the higher
amount of quantified peptides with isoScale is not due to sporadic identifications in one of the replicates, but it is a
consistent improvement as compared to spectral counting. Linear regression analysis demonstrated that extracted ion
chromatogram (XIC), which we used as reference, and isoScale results correlated better than XIC and spectral
counting (Fig. S8A). On average, the variation of the quantification between spectral counting and XIC results was
around 3-fold changes higher as compared to isoScale and XIC, indicating that isoScale provides absolute
quantification more in line to XIC area integration.
isoScale is freely available at: http://middle-down.github.io/Software/
5) Results: comparison of histone H3 marks quantified in crude histone mixture and purified fraction
Fewer steps of histone purification lead to simpler sample preparation and reduced variability of results. Furthermore,
it leads to reduced sample loss, which might be crucial for the analysis of samples with low sample availability. Here,
we briefly show that the results obtained from the analysis of purified histone H3 sample (starting material ~200 µg of
histones) are comparable to the histone H3 quantification from the crude histone mixture, obtained directly from the
TCA precipitation (starting material ~10 µg).
For this aim we performed linear regression between the relative quantification achieved for single histone H3 marks
from the analysis of the two samples (average of the technical replicates). We achieved high correlation (R2: 0.97 for
wt experiment, 0.90 for Suz12-/-) (Fig. 5D). Moreover, we compared the relative abundance of the combinatorial
marks that we identified in both analyses, and we observed a reduced, but not low, correlation (R2: 0.75 for wt, 0.62
for Suz12-/-). Finally, we compared the two sample preparation methods by first generating the ratio of wild type and
Suz12-/- conditions. Specifically, we correlated the ratios (relative abundance Suz12-/- / relative abundance wild type)
of the results obtained from the two sample preparations. We obtained an R2 of 0.72 for the analysis of single marks,
and an R2 of 0.96 for the analysis of combinatorial marks, demonstrating that both analyses provide comparable
results. For instance, by comparing the co-existence frequencies of H3K27 and H3K36 marks we obtained two highly
similar results for both sample preparation analyses (Fig. S8C). In summary, we provide evidence that our workflow
achieves comparable results independently from sample purity.
5
6) Results: comparison of H3K27K36 results with previous publication of Jung et al. (MCP, 2010)
As further validation of our method, we investigated variations in abundance of H3K27 and H3K36 co-occurrences,
due to the possibility to compare it with results previously obtained in our group [9]. In this previous work, we
adopted traditional bottom-up proteomics using SILAC-MS [10], and spectra were acquired using both high and low
resolution for MS/MS with CID and ETD. Results were for 50% in agreement with the previous analysis (Table S10),
even though this previous analysis presented several differences with the middle-down strategy; the work of Jung et
al. was much more focused on K27-K36 PTM co-existence, and it was different in terms of MS strategy and
quantification method. For 5 combinatorial marks total agreement was found between the experiments, while in 5
cases a partial disagreement was found, mostly because in one of the two experiments less than 2-folds changes were
observed between wild type and Suz12-/- cells. Furthermore, we characterized 9 combinatorial marks with the histone
mixture analysis which we could not detect in the work in 2010. However, we found a significant difference in the
quantification of K27ac, which we did not detect as increasing in Suz12-/- cells with the current middle-down analysis.
The wild type/Suz12-/- ratio of this mark was found to be ~1, and no statistical difference was detected. Therefore, we
conclude that our middle-down workflow provides results in line with previous publications, but results with the
bottom-up strategy are still not completely correlated.
7) Results and discussion: middle-down analysis of histone isotypes H3.2/H3.1 and H3.3
Middle-down proteomics is potentially capable of discriminating highly similar histone isotypes, e.g. H3.2 / H3.3 or
H2A type 1 / H2A.x, as the little differences in the amino acid sequence between these variants are included in the Nterminal tails. We did not highlight this aspect in the main article, as we rapidly verified that subdividing the results of
the four canonical histones in their isotypes would have resulted in fewer data and poor statistics. However, we
included a brief description of the analysis of single marks quantified in H3.2 and H3.3 (aa 31 A/S). We observed
significant differences between single marks quantification in H3.2 and H3.3. In particular, we identified 10 marks out
of 43 significantly different in terms of relative abundance in wild type, and 15 out of 43 in Suz12 -/- (Fig. S9 and Table
S11). These marks were mainly low abundance ones such as H3K4 or H3K14/K18/K23 methylations. However, we also
identified H3K9me3 as significantly different between H3.2 and H3.3 in Suz12-/- cells (6.16% for H3.2, 22.36% for H3.3).
In summary, our middle-down workflow is potentially suitable to investigate differences in single and combinatorial
marks residing on the N-terminal tails of histone isotypes such as H3.2 and H3.3.
8) References
[1] Mikesh, L. M., Ueberheide, B., Chi, A., Coon, J. J., et al., The utility of ETD mass spectrometry in proteomic analysis. Biochim
Biophys Acta 2006, 1764, 1811-1822.
[2] Jung, H. R., Sidoli, S., Haldbo, S. N., Sprenger, R. R., et al., Precision mapping of co-existing modifications in histone H3 tails from
embryonic stem cells by ETD-MS/MS. Anal Chem 2013.
[3] Taus, T., Kocher, T., Pichler, P., Paschke, C., et al., Universal and Confident Phosphorylation Site Localization Using phosphoRS.
Journal of Proteome Research 2011, 10, 5354-5362.
6
[4] DiMaggio, P. A., Young, N. L., Baliban, R. C., Garcia, B. A., Floudas, C. A., A Mixed Integer Linear Optimization Framework for the
Identification and Quantification of Targeted Post-translational Modifications of Highly Modified Proteins Using Multiplexed
Electron Transfer Dissociation Tandem Mass Spectrometry. Molecular & Cellular Proteomics 2009, 8, 2527-2543.
[5] Beausoleil, S. A., Villen, J., Gerber, S. A., Rush, J., Gygi, S. P., A probability-based approach for high-throughput protein
phosphorylation analysis and site localization. Nature Biotechnology 2006, 24, 1285-1292.
[6] Cox, J., Neuhauser, N., Michalski, A., Scheltema, R. A., et al., Andromeda: A Peptide Search Engine Integrated into the MaxQuant
Environment. Journal of Proteome Research 2011, 10, 1794-1805.
[7] Savitski, M. M., Lemeer, S., Boesche, M., Lang, M., et al., Confident Phosphorylation Site Localization Using the Mascot Delta
Score. Molecular & Cellular Proteomics 2011, 10.
[8] Pesavento, J. J., Mizzen, C. A., Kelleher, N. L., Quantitative analysis of modified proteins and their positional isomers by tandem
mass spectrometry: Human histone H4. Analytical Chemistry 2006, 78, 4271-4280.
[9] Jung, H. R., Pasini, D., Helin, K., Jensen, O. N., Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient
Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36. Molecular & Cellular
Proteomics 2010, 9, 838-850.
[10] Ong, S. E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., et al., Stable isotope labeling by amino acids in cell culture, SILAC, as
a simple and accurate approach to expression proteomics. Molecular & Cellular Proteomics 2002, 1, 376-386.
[11] Wilkins, M. R., Gasteiger, E., Bairoch, A., Sanchez, J. C., et al., Protein identification and analysis tools in the ExPASy server.
Methods Mol Biol 1999, 112, 531-552.
9) Supplementary figures and tables – captions
Figure S1. Histone H3 purification and analysis. (A) Three aligned UV chromatograms representing elution of intact
histones from C18 column, specificity of purified H3 fraction and reproducibility. The purification of histone H3 was
performed three times to verify reproducibility in separation and retention time of the various intact histone isotypes.
The drift in the retention time was around 1 min at maximum between the three replicates. R.T. = retention time. (B)
emPAI Mascot quantification of the proteins identified in the fraction underlined in (A). Quantification percentage
highlights the high purity of histone H3. (D) Three LC-MS runs from wild type sample of the purified histone H3 tails.
Highly acetylated peptides are eluted first. At minutes ~10 and ~20 shorter peptides are eluted baseline resolved from
histone tails; their relatively higher intensity was cut off the picture for a better visualization.
Figure S2. in-silico GluC digestion of histone H3, H4 and canonical H2A performed by PeptideMass [11]. Histone Nterminal peptides are the largest peptides generated by endoproteinase Glu-C digestion. Note: Only peptides with no
missed cleavage sites and with a mass higher than 500 Da were allowed.
Figure S3. MS/MS spectra and deconvolution. ETD and HCD performance were investigated via direct infusion. ETD
(A) and HCD (B) fragmentation of the peptide ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALRE + 2
methyl groups and respective spectra deconvolution with Xtract (on the right). The figure shows a lower efficiency of
HCD fragmentation (normalized collision energy 35) as compared to ETD.
Figure S4. Deconvoluted and annotated spectra. (A) Fragmentation and c/z ions annotation of the unmodified
histone H3 tail. (B) Fragmentation of the N-terminal acetylated histone H4. (C) Fragmentation of the N-terminal
acetylated histone H2A. c ions were commonly more present than z ions. On the right side of the spectra the most
intense peaks represent the non-fragmented precursor ions with respective neutral losses.
Figure S5. Histone Coder performance for deconvoluted mixed MS/MS spectra. (A) Spectrum containing two histone
H3.2 peptides modified as K27me1 and K36me1. (B) Same spectrum deconvoluted by Xtract. (C) Zoom into the
7
deconvoluted spectrum in the region where site determining ions are located. The ions characterizing H3K27me1 are
highlighted in blue, while ions characterizing H3K36me1 are highlighted in orange. The annotation is only for the five
ions of the same type detected for both peptides. The fragment ions of the two peptides show a delta mass of 14 Da.
(D) Output of the Histone Coder. The two peptides were both identified by Mascot, but with different confidence and
score. However, both were found as reliable by counting the number of site determining ions. In particular, Histone
Coder detected 14 fragment ions confirming me1 localization in K27 instead of K36 (row 2, column 6), and 9 ions
confirming me1 in K36 instead of K27 (row 3, column 6).
Figure S6. Histone H4 and H2A PTM quantification. Quantified PTMs of histones H4 (A) and H2A (B). On the X axis,
modification positions. Modification type is violet: trimethyl (me3), light blue: dimethyl (me2), green: monomethyl
(me1), red: acetyl (ac). On the Y axis, fraction of occupied site on the total histone quantified.
Figure S7. isoScale principle of operation. (A) Logo of the software. (B) MS/MS spectrum of two isobaric peptides. The
black symbols represent the fragments of the species K9me3, the red ones the species K4me1K9me2. (C) Principle of
quantification for a peptide where no co-fragmented isobaric species where found. The total abundance is calculated
by summing all the calculated total ion intensity from each peptide-spectrum match (PSM). (D) Quantification of two
isobaric peptides co-eluted. For each MS/MS spectrum the FIRR was calculated (marked as an asterisk *) and the total
ion intensity was divided by the two co-fragmented peptides before calculating the total abundance. (E) Example of
partially overlapping peptides. The FIRR was calculated only for the MS/MS spectra where the two species where
found as co-fragmented.
Figure S8. isoScale performance. (A) Correlation with XIC quantification for each of the three replicates analyzed. Data
refer to the 22 groups of peptides generated for comparison with XIC (Fig. 3). Results show a higher correlation
between reference (XIC) and isoScale quantification than spectral counting. (B) Correlation of single (left) and
combinatorial (right) marks quantification between the sample containing the crude histone mixture and the one with
purified histone H3. (C) H3K27K36 co-occurrence frequency. Connector lines describe the difference in abundance of
the binary marks between the two cell lines. Green lines represent higher abundance in wt, while red lines in Suz12-/-.
Thicker lines represent higher difference.
Figure S9. Histone H3 isotypes H3.2(H3.1) and H3.3. Abundance of the histone marks identified on the canonical
histone H3 tail (H3.2) and the isotype H3.3. Modification type is violet: trimethyl (me3), light blue: dimethyl (me2),
green: monomethyl (me1), red: acetyl (ac). On the Y axis, fraction of occupied site on the total histone quantified.
Figure S10. Mascot assignment of me3/ac in searches with MS/MS tolerance set as 0.05 Da. MS/MS annotated
spectrum of the histone H3 N-terminal tail modified. Almost every fragment peak resulted as matching theoretical
fragmentation (represented with green dashed lines). Below, delta mass distribution of the different fragment ions
between theoretical and observed masses, expressed in Dalton (top) and ppm (bottom). (A) Wrong assignment of
K9me3, underlined by the constant delta mass of +0.03 Da for the c ion series, starting from the mass corresponding
to the ion c9. (B) Correctly assigned acetylation. By imposing a product ion tolerance of 0.05 Da the search engine
8
could not discriminate two modifications which differ of only 0.03 Da. For both hits on the same spectrum the same
ion score and expect value was assigned.
Figure
S11.
Annotated
spectra.
Mascot
graphical
output
of
annotated
spectra
for
(A)
K4me1K9me2K14acK18acK23acK27me2K36me2, which is the most heavily modified peptide detected, (B)
K9me2K27acK36me3, the lowest scoring peptide which passed bioinformatics validation (Mascot score: 0), (C)
K14me3 and (D) K18me3. On the right, the theoretical fragment ions for each hit. The numbers in bold red are the
ones matching with observed fragment ions in the MS/MS spectrum. Below, the output of the Histone Coder for the
four hits. In the column Modification confidence few more ions are present than the ones in bold red. This is due to
the 30 ppm tolerance for searching site determining ions adopted by Histone Coder, which is more tolerant than 0.01
Da adopted by Mascot.
Table S1. Retention time of histone tails in four technical replicates. Retention time (in min) and coefficient of
variation (% CV) of six groups of peptides. Specifically, we reported the begin and the end of the elution profile for the
injection peak, the short peptides (1-2 kDa), the medium length peptides (3 kDa) and the histone N-terminal tails for
the histone H3, H4 and H2A.
Table S2. Relative quantification of histone H3 N-terminal tails grouped for number of PTM equivalents. The table
contains the values adopted to generate the figure 3A in the main article.
Table S3. Relative abundance of combinatorial histone H3 marks obtained from the purified histone H3 analysis.
The list of combinatorial histone marks quantified in wild type and in Suz12 -/- ESCs was obtained from six replicates.
The relative abundance is calculated by summing the quantification of the same peptide in all six replicates, and then
dividing it by the total amount of histone H3 N-terminal tail quantified. B.D.L. = below detection limit.
Table S4. Relative abundance of combinatorial histone marks from histone H3. Quantified combinatorial PTMs
detected in the crude histone mixture. The results were obtained from four technical replicates.
Table S5. Relative abundance of binary histone marks from histone H3 achieved from the analysis of the purified
histone H3 fraction. The t-test column represents the calculated p-value from an homoscedastic t-test one tail.
Table S6. Relative abundance of binary histone marks from histone H3 achieved from the analysis of the crude
histone mixture. The t-test column represents the calculated p-value from an homoscedastic t-test one tail.
Table S7 and S8. Relative abundance of combinatorial histone marks from histone H4 and H2A. Quantified
combinatorial PTMs detected in the crude histone mixture. The results were obtained from four technical replicates.
Table S9. Output example of decoy database searching. Mascot output of searches that included decoy search for
validation. No middle-down spectra matched a scrambled histone database by chance.
Table S10. Comparison bottom-up and middle-down. Result comparison between data obtained in our previous
work*, and our current analysis performed for the purified histone H3 fraction and the histone mixture. WT and
9
Suz12-/- cells indicate that the co-occurrence is more abundant in wild type cells or Suz12-/-, respectively. White cells
represent less than 2-fold changes regulation. Unmodified sites (unmod) are also considered.
Table S11. Relative abundance of histone marks identified on histone H3.2 and H3.3. Significant changes were
calculated with t-test (5%, one tail, paired). In green, the histone marks detected as significantly changing between
histone H3.2 and H3.3 within the same cell condition.
10
Figure S1
(A)
(B)
H4
H2B
H2A
H3.2
H3.3
H1
H3.1
H3 isolated fraction
>99% specificity
R.T. shift
3 replicates
1 min
(C)
RT: 0.00 - 130.00
NL:
RT: 0.00 -9.39E7
130.00
Base Peak
MS
Obv03151
Shorter peptides
RT: 0.00 - 130.00
3 Ac
2 Ac
1 Ac
NL:
9.39E7
NL: Peak
Base
9.86E7
MS
Base Peak
Obv03151
MS
obv03152
O Ac
NL:
9.86E7
NL: Peak
Base
1.01E8
MS
Base Peak
7000000
obv03152
MS
obv03153
6000000
7000000
6000000
5000000
NL:
1.01E8
4000000
Base Peak
MS
3000000
obv03153
Intensity
Intensity
5000000
4000000
7000000
3000000
6000000
Intensity
2000000
5000000
2000000
1000000
4000000
0
3000000
1000000
0
10
20
30
40
50
60
70
Time (min)
80
70
60
Time (min)
80
90
100
110
120
1000000
0
0
10
20
30
40
50
0
0
2000000
11
90
100
110
120
10
20
30
40
Figure S2
12
Figure S3
(A)
Obv02123 #2827 RT: 95.78 AV: 1 NL: 4.31E3
T: FTMS + p NSI d sa Full ms2 673.90@etd22.50 [100.00-2000.00]
Obv02123_XT_00001_MHp_ #2 RT: 2.00 AV: 1 NL: 7.63E3
T: FTMS + p NSI d sa Full ms2 673.90@etd22.50 [100.00-2000.00]
832.46
z=2
100
1074.83
z=5
1343.28
z=4
90
95
90
895.52
z=6
474.31
z=1
85
85
80
80
75
75
70
65
55
1010.92
z=3
45
719.89
z=2
40
35
983.17
z=5
1286.25
z=4
1148.66
z=3
45
4080.33
40
3442.97
35
205.60
288.14
443.78
z=?
z=?
z=?
346.22
z=?
157.94
z=?
1414.15
z=3
1538.57
z=3
529.83
z=2
1912.81
z=?
1638.61
z=3
2355.38
3029.74
25
20
3720.20
1941.15
30
245.17
z=1
25
5
50
573.34 651.36
z=2
z=2
30
4910.81
55
Xtract
770.17
z=7
50
60
Relative Abundance
Relative Abundance
1361.47
z=3
1229.21
z=4
60
10
1663.92
70
65
15
5339.08
100
ETD
95
828.50
1145.68
20
602.37
4680.71
15
1715.00
z=3
1791.71
z=3
10
1945.56
z=?
3895.25
3314.91
2069.21
997.59
475.32
2625.54
4454.55
5
0
5138.96
4239.46
1438.77
1303.75
3131.80
0
200
400
600
800
1000
1200
1400
1600
1800
2000
500
1000
1500
2000
2500
3000
m/z
3500
4000
4500
5000
m/z
(B)
H3 HCD test_001 #209 RT: 3.00 AV: 1 NL: 2.77E4
T: FTMS + p NSI Full ms2 674.20@hcd30.00 [140.00-1500.00]
H3 HCD test_001_XT_00001_MHp_ #2 RT: 2.00 AV: 1 NL: 2.85E4
T: FTMS + p NSI Full ms2 674.20@hcd30.00 [140.00-1500.00]
673.89
z=?
100
767.91
z=2
1534.82
100
HCD
95
95
90
90
85
85
80
80
75
75
674.40
70
70
65
747.44
z=?
60
55
55
Xtract
50
45
40
35
60
Relative Abundance
Relative Abundance
65
631.37
z=3
211.12
z=1
703.39
z=2
50
45
35
842.47
z=1
30
30
25
25
20
15
842.47
40
776.42
z=2
165.44
z=?
10
603.68
z=3
228.14
z=?
287.13
z=?
346.22
z=?
385.12
z=?
870.01
z=4
776.42
2290.29
15
1003.06 1053.59
z=2
z=?
5
1159.51
z=?
1302.76
1808.04
1406.78
637.38
10
1248.32
z=?
3047.82
1662.91
20
517.95
457.27 z=3
z=?
5
0
0
200
300
400
500
600
700
800
m/z
900
1000
1100
1200
1300
1400
1500
13
400
600
800
1000
1200
1400
1600
m/z
1800
2000
2200
2400
2600
2800
3000
Figure S4
obc04466_xt_00001_mhp_ #2 RT: 2.00 AV: 1 NL: 1.35E5
T: FTMS + p NSI d w Full ms2 668.64@etd22.50 [100.00-2000.00]
(A)
100
95
90
c16
85
Histone H3
80
75
70
c4
Relative Abundance
65
60
55
50
45
c18
40
c10
35
c6
30
25
c7
20
c5
15
c12 c
13
c28
c22
c17
c8
c19
c11
10
z46
c36
c21
c14
c24
c20
5
c32
z31
c31
c27
c26
c35
c43 c44
c41
z45 c47
z48
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
m/z
Obc04462_XT_00001_MHp_ #2 RT: 2.00 AV: 1 NL: 1.46E5
T: FTMS + p NSI d w Full ms2 721.68@etd22.50 [100.00-2000.00]
(B)
100
95
90
85
Histone H4
80
75
70
Relative Abundance
65
60
55
50
c13
45
40
35
30
25
20
15
10
c10
c3
c5
c7
c6
c8
c23 c
26
c14 c
15
c
c16 17
c12
0
500
obc04463_xt_00001_mhp_
#2 1000
RT: 2.00 AV: 1 1500
NL: 1.25E6
T: FTMS + p NSI d w Full ms2 564.57@etd22.50 [100.00-2000.00]
100
z37 z38
c29
c30
c24
c22
c19
5
c32 c34
2000
z45
c35
2500
3000
m/z
3500
4000
4500
z47
z48
5000
5500
(C)
95
90
85
80
Histone H2A
75
70
Relative Abundance
65
60
55
50
45
40
35
30
25
c4
20
15
10
c5 c c8
6
z20 c c24
c16
c7
c9
23
c21
c11
c c14
z9 c12 13 c15
c17
c19 c20
z22
z21
z33
c27 c28
z30 c33 z32
5
0
500
1000
1500
2000
2500
m/z
14
3000
3500
4000
4500
Figure S5
(A)
(B)
Obv02119 #3308 RT: 116.43 AV: 1 NL: 2.89E4
T: FTMS + p NSI d sa Full ms2 670.27@etd22.50 [100.00-2000.00]
Obv02119_XT_00001_MHp__140429121930 #2 RT: 2.00 AV: 1 NL: 5.10E4
T: FTMS + p NSI d sa Full ms2 670.27@etd22.50 [100.00-2000.00]
5311.07
5353.09
832.47
z=2
100
100
95
1069.02
z=5
90
95
90
85
1053.86
z=?
893.52
z=6
80
85
474.31
z=1
80
5296.05
75
766.16
z=7
70
1663.92
75
70
65
5356.10
4881.79
5267.04
5252.01
5110.93
4895.82
4953.86
4066.37
4196.40
4210.43
4372.56
4427.55
4439.53
4581.65
4653.69
4682.71
4753.73
5367.10
3691.17
3414.95
4053.35
3868.24
3916.32
3995.34
3700.15
3428.96
3563.08
3707.20
2873.62
2731.55
2482.49
2624.55
3001.72
1941.16
1303.75
5
3248.91
3285.88
3349.97
10
2345.30
15
1945.56
z=?
3014.73
3088.74
1705.33 1781.37
1890.21
z=3
z=3
z=?
2069.21
2113.22
1552.90
z=3
1948.11
1434.16
z=3
177.05
z=?
0
100
1628.94
z=3
2254.34
5
1404.81
z=3
529.83
z=2
1912.86
z=?
1607.91
10
465.78
288.14
z=2
z=?
346.22 401.23
z=?
z=?
982.55
25
20
226.55
z=?
15
602.37
751.78
765.59
774.46
30
20
1656.96
35
1279.24
z=4
903.52
930.56
245.17
z=1
1792.01
40
924.05
z=4
2354.38
45
1139.66
z=3
30
25
50
245.17
35
55
475.32
573.35
648.06
z=2
z=3
719.89
z=2
40
60
1813.06
977.56
z=5
1488.87
1329.53
z=4
739.24
z=5
45
1664.94
50
1145.68
1221.96
z=4
1438.78
55
Relative Abundance
Relative Abundance
65
60
0
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
500
1000
1500
2000
2500
3000
m/z
3500
4000
4500
5000
m/z
2069.21
Obv02119_XT_00001_MHp__140429121930 #2 RT: 2.00 AV: 1 NL: 6.73E3
T: FTMS + p NSI d sa Full ms2 670.27@etd22.50 [100.00-2000.00]
me1
(C)
100
(z+1)18
ARTKQTARKSTGGKAPRKQLATKAAR K S A P A T G G V K KPHRYRPGTVALRE
95
c28
90
c31 c32 c33
(z+1)18
85
me1
ARTKQTARKSTGGKAPRKQLATKAAR K S A P A T G G V K KPHRYRPGTVALRE
80
3001.72
Obv02119_XT_00001_MHp__140429121930 #2 RT: 2.00 AV: 1 NL: 1.38E4
T: FTMS + p NSI d sa Full ms2 670.27@etd22.50 [100.00-2000.00]
c28
65
50
45
40
(z+1)18
25
60
20
55
15
(z+1)18
50
2020
2040
2060
2080
2100
2120
25
2140
m/z
2160
5
c33
3363.96
3299.87
10
c31
3234.91
3218.87
3115.79
3088.74
15
2180
c33
c31
c28
20
c32
3392.98
c28
3272.85
30
0
2000
3248.91
35
c32
3285.88
5
3014.73
40
2986.70
10
45
2177.21
2179.77
30
65
3400.93
2005.13
2006.20
35
70
0
3000
3050
3100
3150
3200
m/z
3250
3300
3350
3400
(D)
Mascot score
Expection value
Scan number
Histone code
Run name
Site det. ions
129.77
5.7E-12
3308
K27me1
WT rep3
|2-K27me1-14|
111.53
3.8E-10
3308
K36me1
WT rep3
|9-K36me1-3|
15
3406.97
75
3349.97
80
55
3335.95
85
2082.25
2084.22
90
2113.22
60
2019.15
95
Relative Abundance
c31 c32 c33
70
Relative Abundance
100
75
2200
2220
Figure S6
(A)
H4 Suz12-/-
relative occupancy
H4 Wild type
S1
S1
(B)
H2A Suz12-/-
relative occupancy
H2A Wild type
S1
K125
16
S1
K125
Figure S7
Obv02232_XT_00001_MHp_ #2 RT: 2.00 AV: 1 NL: 2.18E3
T: FTMS + p NSI d sa Full ms2 683.77@etd22.50 [100.00-2000.00]
(B)
474.31
(A)
100
K4
me3
A R T K Q T A R K …
95
me2
A R T K Q T A R K …
602.37
85
80
1100.70
me1
90
K9-K9*
Q5
75
774.46
70
65
A7
K4*
1085.13
1010.60 1021.60
1022.97
959.56
967.57
5
984.60
995.59
769.45
T6*
801.79
718.44
10
600.36
571.33
15
918.04
A7*
20
934.07
Q5*
842.52
25
859.52 865.86
30
1024.36
1030.61
1063.42
1064.88
1068.63
866.19
T6
T3
827.85
839.98
35
616.39
40
346.22
45
897.53
703.42
50
877.84
895.14
903.54
488.33
55
788.47
Relative Abundance
60
0
350
400
450
500
550
600
650
700
750
800
850
900
950
1000
1050
1100
m/z
(D)
Peptide with no isobaric species
400
500
peptide 1
peptide 2
350
300
250
206
200
150
100
50
0
Retention time
TOTAL
Calculated total ion current (arbitrary units)
Calculated total ion current (arbitrary units)
450
(E)
Co-eluting isobaric peptides
500
457
450
peptide 1
400
peptide 2
350
300
250
206
200
150
*
100
50
0
*
* *
*
*
*
*
Retention time
* *
*
TOTAL
500
Calculated total ion current (arbitrary units)
(C)
450
400
Partially co-eluting isobaric peptides
457
peptide 1
350
300
250
206
200
150
*
100
50
0
* *
* *
Retention time
*a single MS/MS spectrum, where the FIRR between peptide 1 and 2 was calculated
17
peptide 2
*
*
*
TOTAL
Figure S8
(A)
(B)
Single marks
Replicate 1
Spectral counting
0.57
isoScale
1
0.90
Replicate 2
0.61
0.69
Replicate 3
0.66
0.88
R2 correlation
R2
0.97
0.9
0.75
0.5
0.25
0
Wild type Suz12-/-
Histone mixture
18
0.75
0.62
0.5
0.25
Purified H3
1
0.75
0
(C)
Combinatorial
marks
Wild type Suz12-/-
relative occupancy
relative occupancy
H3.3
relative occupancy
H3.2
relative occupancy
Figure S9
wild type
Suz12-/-
19
Figure S10
(A)
(B)
20
Figure S11
(A)
(B)
(C)
(D)
Figure
Histone code
A
K4me1K9me2K14acK18acK23acK27me2K36me2
B
K9me2K27acK36me3
C
K14me3
D
K18me3
Site determining ions
Modification confidence
K4me1{R2:2/4(c:2;z+1:48;)-R8:4/8(c:4;c:5;c:6;z+2:45;)}
K9me2{R8:2/2(c:8;z+2:42;)K14:9/10(c:10;c:11;c:12;c:13;z+2:37;z+2:38;z+2:39;z+2:40;z+2:41;)}
K14ac{K9:9/10(c:10;c:11;c:12;c:13;z+2:37;z+2:38;z+2:39;z+2:40;z+2:41;)P16:1/4(z+1:36;)} K18ac{P16:4/4(c:16;c:17;z+2:33;z+2:34;)K23:8/10(c:18;c:19;c:21;c:22;z+2:28;z+2:29;z+2:31;z+2:32;)}
|2-K4me1-4||2-K9me2-9||9-K14ac-1||4-K18ac-8|
K23ac{K18:8/10(c:18;c:19;c:21;c:22;z+2:28;z+2:29;z+2:31;z+2:32;)|8-K23ac-8||3-K27me2-12||12-K36me2-2|
K27:8/8(z+1:24;z+2:24;c:23;c:24;z+1:26;z+2:26;z+1:27;c:26;)}
K27me2{R26:3/2(z+1:24;z+2:24;c:26;)K36:12/18(z+1:16;z+1:17;z+1:18;z+2:18;z+1:19;z+2:23;c:27;c:28;c:32;c:33;c
:34;c:35;)}
K36me2{K27:12/18(z+1:16;z+1:17;z+1:18;z+2:18;z+1:19;z+2:23;c:27;c:28;c
:32;c:33;c:34;c:35;)-K37:2/2(z+1:14;c:36;)}
K9me2{R8:2/2(c:8;z+1:42;)K14:6/10(c:10;c:11;c:13;z+1:37;z+2:39;z+2:41;)} K27ac{K23:1/8(z+1:26;)|2-K9me2-6||1-K27ac-1||3-K36me3-2|
P30:1/6(c:27;)} K36me3{K27:3/18(z+1:15;z+2:18;c:27;)K37:2/2(z+1:14;z+2:14;)}
K14me3{K9:6/10(c:10;c:12;c:13;z+2:37;z+1:40;z+2:41;)|6-K14me3-6|
K18:6/8(c:14;c:16;c:17;z+1:33;z+2:34;z+2:36;)}
|4-K18me3-10|
21
K18me3{K14:4/8(c:14;c:16;c:17;z+2:34;)K23:10/10(c:18;c:19;c:20;c:21;c:22;z+1:28;z+2:28;z+1:29;z+1:31;z+1:32;)}
Table S1
Injection peak
1-2 kDa peptides
3 kDa peptides
histone H3 tails
histone H4 tails
histone H2A tails
histone H2B tails
replicate 1
begin
5.6
18.8
29.1
87.8
89.9
97.8
111.3
end
11.3
29.1
84.8
126.0
141.9
124.4
125.7
replicate 2
begin
5.5
18.8
28.8
88.2
98.7
97.6
110.8
end
11.0
28.8
84.2
125.7
137.3
125.3
126.5
replicate 3
begin
5.5
18.7
29.1
89.6
101.4
96.0
109.7
Table S2
PTMs
unmod
1me
2me
3me
4me
5me
6me
7me
8me
9me
1ac
1ac1me
1ac2me
1ac3me
1ac4me
1ac5me
1ac6me
2ac
2ac1me
2ac2me
2ac3me
3ac
XIC
1.88%
1.62%
5.02%
9.98%
14.01%
14.82%
8.59%
2.49%
0.33%
0.00%
1.73%
1.81%
4.02%
6.18%
8.52%
7.07%
4.30%
1.28%
1.32%
2.09%
2.93%
0.00%
Spectral counting
8.01%
4.27%
9.60%
16.68%
15.32%
8.98%
4.92%
1.54%
0.16%
0.14%
3.21%
2.28%
4.58%
5.31%
7.08%
4.25%
1.01%
0.62%
0.50%
0.65%
0.77%
0.12%
isoScale
3.10%
1.90%
4.47%
11.19%
16.18%
9.69%
8.66%
2.69%
0.07%
0.09%
3.38%
1.80%
3.37%
5.57%
12.88%
6.28%
3.41%
0.77%
1.39%
1.51%
1.47%
0.14%
22
end
11.2
29.1
84.5
126.6
139.2
127.0
125.4
replicate 4
begin
5.5
18.7
29.3
88.7
103.2
94.9
108.8
end
11.1
29.3
84.5
125.4
138.6
125.3
128.0
% CV begin
1.05%
0.17%
0.71%
0.91%
6.01%
1.43%
1.03%
% CV end
1.16%
0.71%
0.33%
0.40%
1.40%
0.87%
0.90%
Table S3
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
K9me1K14me1K27me2K36me2
12.23%
1.69%
K9acR17me2K27me2K36me2
0.34%
0.23%
K4me3K23acK27me2K36me2
0.04%
B.D.L.
K27me2K36me2
9.31%
B.D.L.
K23acK27me3
0.16%
0.05%
K9acK14me1K18acK23acK27me2K36me2
0.04%
B.D.L.
K9me1K27me2K36me2
8.39%
B.D.L.
K14acK18acK23acK27me2K36me2
0.11%
B.D.L.
K18acK27me2K36me2
0.04%
B.D.L.
K9me3K27me2K36me2
6.19%
1.68%
K4me1K9me1K18acK23acK27me2K36me2
0.11%
B.D.L.
R17me1R26me1K36me2
0.04%
B.D.L.
K9me1K14me1K23acK27me2K36me2
4.30%
0.18%
K14acK27me3K36me3
0.10%
B.D.L.
K4me1K23acK27me2K37me1
0.04%
B.D.L.
K23acK27me2K36me2
3.95%
0.02%
K18me1K23me1K27me1K36me2
0.10%
B.D.L.
K9me2K14acK18acK23acR42me2R49me2
0.04%
B.D.L.
K9me1K23acK27me2K36me2
2.99%
B.D.L.
K9me1K14me1K27me3K36me2
0.10%
B.D.L.
K9me1K14acK18acK23acK27me3
0.04%
B.D.L.
K27me2K36me1
1.72%
0.01%
K9me2K23me3K27acK36me1K37me1
0.10%
B.D.L.
K9me2K27me3R40me1
0.04%
B.D.L.
K23acK27me2K36me1
1.56%
0.15%
K14me1R17me1K27me2K36me2
0.10%
B.D.L.
K9me1K36me3
0.04%
B.D.L.
(unmodified)
1.41%
3.01%
K14me1K18acK23acK27me2K36me2
0.10%
B.D.L.
K4me1K9me1K14acR17me1K23acR26me1K27me1K36me1K37me1
0.04%
B.D.L.
K9me2K27me3K36me1
1.32%
0.04%
K9me3K23me2K27me2K36me2
0.09%
B.D.L.
K9me2K27me3R49me2
0.04%
K14acK18acK27me2K36me2
1.27%
B.D.L.
K14me1K18me1K23acK27me2K36me2
0.09%
B.D.L.
K9me2K14acK18acK23acK27me1K36me1K37me1
0.04%
B.D.L.
K9acK14me1K18me1K23acK27me2K36me2
1.26%
B.D.L.
K14acK23me3R26me1K27me3
0.09%
B.D.L.
K23acK27ac
0.05%
0.01%
K9me1K14acK27me2K36me2
1.17%
B.D.L.
K9acK18me1K23me2K27me2K36me2
0.09%
B.D.L.
K27me1K36me3
0.04%
B.D.L.
K9me3K14acK27me2K36me2
1.95%
0.81%
K9me2K23me3K27ac
0.09%
B.D.L.
K18me1K27me2K36me2
0.04%
B.D.L.
K27me3K36me2
1.08%
B.D.L.
K9me2K14acK18me1K23me1K27acK36me3
0.08%
B.D.L.
R8me1K9acR17me1K23me2R26me1K27me2K36me2
0.04%
B.D.L.
K9me3K23acK27me2K36me2
1.02%
B.D.L.
K18me1K23me1K27acK36me2
0.08%
B.D.L.
R8me1K14acK27me2K36me2
0.03%
B.D.L.
K9me1K14acK18acK27me2K36me2
0.90%
B.D.L.
K4me1K9me1K14acK23acK27me3K36me1K37me1
0.08%
B.D.L.
R17me2K27me2K36me2
0.04%
0.00%
K9me3K14acK18acK27me2K36me2
0.87%
B.D.L.
K9me2K14acK23me3K27me1K36me1K37me1
0.08%
B.D.L.
K9me1K14me1R26me1K27me2K36me2
0.03%
B.D.L.
K14acK18acK27me2
1.12%
0.28%
R26me1K27me1K36me1
0.08%
B.D.L.
K4me3K27me2K36me2
0.03%
B.D.L.
K9me2K27me3K36me1K37me1
0.80%
0.03%
K27me2R40me2
0.08%
B.D.L.
K14acK18me2K23acK27me1K36me1
0.03%
B.D.L.
K27me1K36me2
1.04%
0.28%
K9me2K18acR26me2R40me2
0.07%
B.D.L.
K9me3R26me1K27me2K36me2
0.04%
0.00%
K23acK27me2
1.03%
0.28%
K9me3K27me2K36me1K37me1
0.16%
0.08%
K9me2K23me1K27me1K36me3
0.03%
B.D.L.
K14acK18me1K23acK27me2
0.71%
B.D.L.
K9acK14me1K18acK27me2K36me2
0.07%
B.D.L.
K9me3R26me2K27me1K36me1K37me1
0.03%
B.D.L.
K14me1K27me2K36me2
0.59%
B.D.L.
K4me1R8me1R17me1K27me2K36me2
0.07%
B.D.L.
K14acK18me1K23acK36me3
0.03%
B.D.L.
K9me1K14me1K18acK27me2K36me2
0.58%
B.D.L.
K18acK23acK27me2K36me1
0.07%
B.D.L.
K23acR26me1K36me3
0.03%
B.D.L.
R8me1K9me1K14me1K27me2K36me2
0.58%
0.05%
K9me2K23acK27me3
0.07%
B.D.L.
K9me1K27me2R40me1R42me1
0.03%
B.D.L.
K23me1K27me1K36me2
0.51%
B.D.L.
K9me2K18acK23acK27me1K36me1K37me1
0.07%
B.D.L.
K9me3K23me2R26me2K27me2K36me2
0.03%
B.D.L.
K14acK27me2K36me2
0.48%
B.D.L.
R8me1K9me1K23me2K27me2K36me2
0.07%
B.D.L.
K9me2K18acK27me3
0.03%
B.D.L.
R8me1K9me1R17me1K27me2K36me2
0.39%
B.D.L.
K23acK27me3K36me1K37me1
0.07%
B.D.L.
K4me1K27me2K36me2
0.03%
B.D.L.
K23acR26me1K27me2K36me2
0.39%
B.D.L.
R8me2K14acK18acK27me2K36me2
0.07%
B.D.L.
K9me1K18acK23acK27me3
0.03%
B.D.L.
K9me1R17me1K27me2K36me2
0.35%
B.D.L.
K9me3K18acK23acK27me2K36me2
0.07%
B.D.L.
R8me1K23acK27me1K36me1K37me1
0.03%
B.D.L.
K9me1K14acK18acK23acK27me2K36me2
0.34%
B.D.L.
K9me3K14me3K27me2K36me2
0.07%
B.D.L.
K9acK23me3K27me3K36me2
0.03%
R26me1K27me2K36me2
0.33%
B.D.L.
K4me1K9me1K18acK27me2K36me2
0.08%
0.01%
R26me2K27me1K36me1K37me1
0.03%
B.D.L.
K23acR26me1K27me1K36me1
0.32%
0.01%
K9me1K14acK23me2K27me2K36me2
0.07%
B.D.L.
K4me1K9me1K14acK18acK23acK27me2K36me2
0.07%
0.04%
K9me3K18acK27me2K36me2
0.34%
0.05%
K14acK18acK27me3
0.07%
B.D.L.
K4me2K23acK27me3K36me1K37me1
0.03%
B.D.L.
K23acK27me2K36me3
0.28%
B.D.L.
K9acK27me2K36me2
0.06%
B.D.L.
K23acK27me1K36me3
0.03%
B.D.L.
K27me1K36me1
0.32%
0.05%
K9me1K23acK27me3
0.06%
B.D.L.
K4acR8me1K27me2K36me2
0.03%
B.D.L.
K9me1K23acK36me3
0.26%
B.D.L.
K14acK18acR26me1K27me1K36me1
0.06%
B.D.L.
K14acR17me1K18acK27me2K36me2
0.03%
B.D.L.
K9me1K18acK23acK27me2K36me2
0.26%
B.D.L.
K23acK27me2K36me1K37me1
0.06%
B.D.L.
K9acK14acK23me2R26me1K27me2K36me2
0.03%
B.D.L.
K27me3
0.45%
0.20%
K27me3K36me1K37me1
0.06%
B.D.L.
K9me3K14me3K23acK27me2K36me2
0.03%
B.D.L.
K9me3K14acK18acK23acK27me2K36me2
0.24%
B.D.L.
R8me2K18acK27me2K36me2
0.08%
0.02%
K9me1K14acK18acK36me3
0.03%
B.D.L.
K9acK14me1K18me1K27me2K36me2
0.23%
B.D.L.
K9acR17me1K27me2K36me2
0.06%
B.D.L.
K14acK18me2K23acK27me3
0.03%
B.D.L.
K9me2K27me3R40me2
0.22%
B.D.L.
K14acK18me1K23me1K27ac
0.06%
B.D.L.
K9me1K18acK23me2K27me2K36me2
0.03%
B.D.L.
R8me2K14acK23me2K27me2K36me2
0.21%
B.D.L.
K27me2K36me3
0.06%
B.D.L.
K18acK23acK27me1K36ac
0.03%
B.D.L.
K4me1K23acK27me2K36me2
0.20%
B.D.L.
R8me2R26me1K27me2K36me2
0.06%
B.D.L.
R17me1K18acK27me2K36me2
0.03%
B.D.L.
K14acK23me3K27me2K36me2
0.20%
B.D.L.
K9me2K23acK27me1K36me1K37me3
0.06%
B.D.L.
K18acK23acK27me3
0.03%
B.D.L.
K14acK23me3K27me3
0.20%
B.D.L.
K9me2K23me3K27me3K36me1
0.06%
B.D.L.
R17me1K23me2K27me2K36me2
0.03%
B.D.L.
K4me1K9me1K27me3K36me2
0.19%
B.D.L.
K14acK27me1
0.06%
B.D.L.
K14acK23me1K27me1K36me2
0.03%
B.D.L.
K14acK18me1K23acK27me2K36me2
0.19%
B.D.L.
K9me2K23acK27acR49me2
0.06%
B.D.L.
R26me1K36me1
0.03%
0.01%
K14acK18me1K23me2K27ac
0.17%
B.D.L.
K23acR26me2K27me1K36me1
0.06%
B.D.L.
K9me1K14me1K23me2K27me2K36me2
0.02%
B.D.L.
R26me2K36me2
0.15%
B.D.L.
K4me1K9me1R26me1K27me2K36me2
0.06%
B.D.L.
K9me1K18acK23acK27me2
0.02%
B.D.L.
K9me1K14me1K18acK23acK27me2K36me2
0.15%
B.D.L.
K14acK23me2K27me3
0.06%
B.D.L.
K9acK14me1K18acK23acK27me3
0.02%
B.D.L.
K18acK23acK27me2K36me2
0.14%
B.D.L.
K4me3K14acK27me2K36me2
0.06%
B.D.L.
K18acK23acK27acK36me2
0.02%
B.D.L.
K14acR17me2K27me2K36me2
0.22%
0.08%
K9me3K27me2K36me2K37me1
0.07%
0.02%
K9me2K23me3K27me3
0.02%
B.D.L.
K14me1K23acK27me2K36me2
0.14%
B.D.L.
K9me2K23acK27me1K36ac
0.14%
0.09%
K14acK18acK23acK27me1K36me3
0.02%
B.D.L.
K14acK23me3K27me2
0.14%
B.D.L.
K4me1K9me1K23acK27me2K36me2
0.08%
0.03%
R8me1K14acR17me1K18acK27me3
0.02%
B.D.L.
K4me1K9acK23acK27me2K36me1K37me1
0.13%
B.D.L.
K4me1K9me2K23acK27me3K36me1K37me1
0.05%
B.D.L.
K9acK14me3K27me2K36me2
0.02%
B.D.L.
K27me3K36me1
0.13%
B.D.L.
R8me1K23acK27me2K36me2
0.05%
B.D.L.
K4acK9me1K36me3
0.02%
B.D.L.
R8me2K18acK23acK27me2K36me2
0.13%
B.D.L.
R8me1K27me2K36me2
0.05%
B.D.L.
K9me2K14acK18acK23acK27me1K36me1K37me3
0.02%
B.D.L.
K9me2K27me3R40me1R42me1
0.13%
B.D.L.
K5acK15me1
0.11%
0.06%
K9me1R26me1K36ac
0.02%
B.D.L.
R8me1K18acK23acK27me2K36me2
0.12%
B.D.L.
K18acK23acK27me2
0.10%
0.05%
K9me3K23acK27ac
0.02%
B.D.L.
K14acK18acK27me1
0.36%
0.24%
K9acK23me3K27me2K36me2
0.05%
B.D.L.
K4me1K9me1K14acK27me3K36me1K37me1
0.02%
B.D.L.
K9acK14me1K27me2K36me2
0.12%
B.D.L.
K27me2R49me2
0.05%
B.D.L.
K14acK23me3K27me1
0.09%
0.07%
K4me1K9me1K14acK23acK27me2K36me1K37me1
0.12%
B.D.L.
K14acK18acK27me2K36me1
0.05%
B.D.L.
K14acK27me2
0.02%
B.D.L.
K9acK14me1K18me1K23me1K36me2
0.12%
B.D.L.
R17me1K27me2K36me2
0.05%
B.D.L.
R26me2K36ac
0.02%
B.D.L.
K9me2K23acR42me2R49me2
0.13%
0.02%
K18me1K23me1K36me2
0.05%
B.D.L.
K14acK23me3K27me3K36me1
0.02%
B.D.L.
K4me1K9me2K27me3K36me1K37me1
0.11%
B.D.L.
K9me1K23me2K27me2K36me2
0.05%
B.D.L.
K4me1R8me1K9me1K27me3K36ac
0.02%
B.D.L.
K4me1K9me1K14me1K27me3K36me2
0.11%
B.D.L.
K14acK18acK23acK27me2
0.05%
B.D.L.
K9acK23me2R26me2K27me2K36me2
0.02%
B.D.L.
K27me2K36me1K37me1
0.11%
B.D.L.
K9me1R26me1R42me2
0.04%
B.D.L.
K9acK14acK23me2K27me2K36me2
0.02%
B.D.L.
K18me3K36me2
0.02%
B.D.L.
K14me3K18acK23acK27me2K36me2
0.01%
B.D.L.
K5acR17me1
0.00%
0.00%
K9me1R26me1K36me3
0.02%
B.D.L.
K20ac
0.01%
B.D.L.
R17me1R26me2K27ac
B.D.L.
0.00%
R17me1K23acK27me2K36me2
0.02%
B.D.L.
K9me2K27me3R49me1
0.01%
B.D.L.
K23acR49me1
B.D.L.
0.00%
K23acR26me2K36me3
0.02%
B.D.L.
K27me3R40me2
0.01%
B.D.L.
K9me2R17me1K36me3
B.D.L.
0.00%
K23acR26me2R40me2
0.02%
B.D.L.
K27me1R42me2
0.01%
B.D.L.
K4me1K36me2
B.D.L.
0.00%
K4acK27me3
0.02%
B.D.L.
K9me1K14acK36me3
0.01%
B.D.L.
K23acR49me2
B.D.L.
0.00%
K9me2K27me1K36me3
0.61%
0.59%
K14acK36me1
0.01%
B.D.L.
K9me3K14me3K18acK36me2
B.D.L.
0.00%
K9acK14me2K18acK23acK27acR42me2
0.02%
B.D.L.
K9me3K14me1K18me3K27me2K36me2
0.01%
B.D.L.
K9me1K14acK18acR26me2
B.D.L.
0.00%
K9me2K18acK23acK27me3
0.02%
B.D.L.
K23acK27me1K36ac
0.01%
B.D.L.
R26me2
B.D.L.
0.00%
K23acK27me2K36ac
0.02%
B.D.L.
K23acK37me2
0.01%
0.00%
K14acK36me2
0.00%
0.01%
23
B.D.L.
B.D.L.
Table S3 - continues
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
K14acK18me2K23me2K27me1K36ac
0.02%
B.D.L.
K9acK18me2K23me2K27me2K36me2
0.01%
B.D.L.
K18acK27me1K36me1K37me1
B.D.L.
0.00%
K4me3K27me2K36me1
0.02%
B.D.L.
K18acK36me1
0.01%
0.00%
K9me2K18acK27me1
B.D.L.
0.00%
K9me2K27acR40me2
0.02%
B.D.L.
K4me1K14acK18acK23acK27acK36me2
0.01%
B.D.L.
K9me2K23acR26me2R40me2
0.02%
0.03%
K14acK18acK23me1K27me2
0.02%
B.D.L.
K27me2R42me2
0.01%
B.D.L.
K9me3R49me2
B.D.L.
0.00%
K9me2K18me1K23me1K36me3
0.02%
B.D.L.
R17me1R26me1K27me1K36me1
0.01%
B.D.L.
K23me3K36me2
0.00%
0.01%
K14acK18acK23acK27me1
0.02%
B.D.L.
K27me3R40me1
0.01%
B.D.L.
K9me2K23me1K36me3
B.D.L.
0.00%
K9me1K23me3K27me2K36me2
0.02%
B.D.L.
K9me1K14me2K27ac
0.01%
B.D.L.
K9me3K36me2K37me2
B.D.L.
0.00%
K14acK18me1K23me2K27me2K36me2
0.02%
B.D.L.
K14acK18acK23acK36me2
0.01%
B.D.L.
K23me2K36me1
B.D.L.
0.00%
K14me1K18me1K27me2K36me2
0.01%
B.D.L.
K9me1K27me3
0.01%
B.D.L.
K9me3K23me1K36ac
B.D.L.
0.00%
K9acK14me1K18acK23me2K27me2K36me2
0.01%
B.D.L.
K18acK23me1K27me1K36me1
0.01%
B.D.L.
K9me1R26me2
B.D.L.
0.00%
K14acK18me2K23acK36me1K37me1
0.01%
B.D.L.
K4me1K9me1K23acK27me3K36me1K37me1
0.01%
B.D.L.
R17me1K27me1
B.D.L.
0.00%
K14acK18acR26me1K27me2K36me2
0.01%
B.D.L.
K9me3K27me2K36ac
0.00%
B.D.L.
K9acK14me3K18me3K36me2
B.D.L.
0.00%
K14acK18acR26me2R40me2
0.01%
B.D.L.
K14acK23me1K27me1K36me1
0.00%
B.D.L.
K9me2K23me1R49me2
B.D.L.
0.00%
K14acK18me2K23me1K27ac
0.01%
B.D.L.
K27me3K37me2
0.00%
B.D.L.
K18acK27me1
0.01%
0.01%
K9acK23me3K27me3
0.01%
B.D.L.
K18acK27me2
0.00%
B.D.L.
K9me2K27acR49me2
0.02%
0.03%
K23acK27me2K37me3
0.01%
B.D.L.
K14me3K27me2K36me1
0.00%
B.D.L.
K9me2K27me3K36ac
B.D.L.
0.01%
K14acR26me1K27me1K36me1
0.01%
B.D.L.
K18acK23acR26me1K27me3
0.00%
B.D.L.
K9me2R26me2K36me1
0.00%
0.01%
K23acK27acK36me1
0.01%
B.D.L.
K23acR26me2K36me1
0.00%
B.D.L.
K4me1K9me2K14acK27me1K36me1K37me1
B.D.L.
0.01%
K14acK18me2K23acK36me3
0.01%
B.D.L.
K27me1R49me2
0.00%
B.D.L.
K9me1K14me1R40me1R42me1
B.D.L.
0.01%
K9me3K18me2K23me2K27me2K36me2
0.01%
B.D.L.
K18acK23acK36me2
0.03%
0.02%
K9me2K23ac
B.D.L.
0.01%
K18acK23me3K27me1
0.01%
B.D.L.
K9me1K23me2R26me1K27me2K36me2
0.00%
B.D.L.
K14me1R17me2
B.D.L.
0.01%
K4me1K14acK18acK23acK27me2K36me2
0.01%
B.D.L.
K23me3K27me3K36me2
0.00%
B.D.L.
K23me3K36ac
B.D.L.
0.01%
K18me1K23acK27me2K36me2
0.01%
B.D.L.
K23me2K27acK36me1
0.00%
B.D.L.
R42me1
B.D.L.
0.01%
K9me1K18acK27me2K36me2
0.01%
B.D.L.
R26me1K27me1R40me2
0.00%
B.D.L.
K4me1K9me1K23acK27me1K36me1K37me1
B.D.L.
0.01%
K14acK23me2R26me1
0.01%
B.D.L.
R26me1K27me1
0.01%
0.00%
K9acK36me3
B.D.L.
0.01%
K23me1K27me2K36me2
0.01%
B.D.L.
K9me1R26me1K27me3
0.00%
B.D.L.
K9me2K23me1K36ac
B.D.L.
0.01%
K9me1K23acK27acR42me1R49me1
0.01%
B.D.L.
K27me3R42me1
0.00%
B.D.L.
K9me2K14acK18acK23acR49me2
B.D.L.
0.01%
K9me1K14acK27me3
0.01%
B.D.L.
K9me2K23acK27acR40me2
0.00%
B.D.L.
R17me2
B.D.L.
0.01%
K18acK23me3K27me3
0.01%
B.D.L.
K9me1K18acK27me2
0.00%
B.D.L.
K9me2K18me1K36me3
B.D.L.
0.01%
K9acK14acK18me1K23me1K27acK36me2
0.01%
B.D.L.
K14acK18me1K27me2
0.00%
B.D.L.
R8me2K23acK27me2K36me2
0.06%
0.06%
K14acK27me2K36me1
0.01%
B.D.L.
K4me1R8me1K27me2K36me2
0.00%
B.D.L.
R8me1K9me1K36me3
B.D.L.
0.01%
R8me1K23me1K36me3
0.01%
B.D.L.
K23acR26me2K27me1K36me1K37me1
0.00%
B.D.L.
K14me2K27me1K36me1
B.D.L.
0.01%
K4me1K9acK36me2
0.01%
B.D.L.
K18acK27me3K36me3
0.00%
B.D.L.
K9me2K37me3
B.D.L.
0.01%
K14acK18acK36me1
0.01%
B.D.L.
K18acR26me1K27me2K36me2
0.00%
B.D.L.
R17me1K23me1K27me1K36ac
B.D.L.
0.01%
K14acK18acR26me2K36me3
0.01%
B.D.L.
K14me3K36me2
0.00%
B.D.L.
K9me3K18acK27me1K36me1K37me1
B.D.L.
0.01%
K9me3K27acK36me1K37me1
0.01%
B.D.L.
K23me3
0.00%
0.00%
K9me3K23acK27me2K36ac
B.D.L.
0.01%
K9me1K14me1K18me1R26me1K27me2K36me2
0.01%
B.D.L.
K18acK23acR26me1K27me1K36me1
0.00%
B.D.L.
K14me3K23acK27me1K36me1K37me1
B.D.L.
0.01%
K4me1K9me1K14acK23acK27me1K36me2K37me1
0.01%
B.D.L.
K23me2K27ac
0.00%
B.D.L.
R17me1K23acR26me2
B.D.L.
0.01%
R26me1K27me1K36me1K37me1
0.01%
B.D.L.
K14acK18me1
0.00%
B.D.L.
K9me1K14me1
B.D.L.
0.01%
K9me2K37me1R49me2
0.01%
B.D.L.
K23acK27me1K37me3
0.00%
B.D.L.
K9me2K14acK27me1
B.D.L.
0.01%
K14me3K23acK27me2K36me2
0.01%
B.D.L.
K14acK23me1
0.00%
B.D.L.
K9me2K14me2R17me1
B.D.L.
0.01%
K23acK37me3
0.01%
B.D.L.
K27me2K37me1
0.00%
B.D.L.
K9me1K23acR42me1R49me1
B.D.L.
0.01%
K15me1
0.20%
0.19%
R26me1K27me2
0.01%
0.01%
K9me1R40me2
B.D.L.
0.01%
K23me2K27me2K36me2
0.01%
B.D.L.
K9acR49me2
B.D.L.
0.00%
K9me1K23me2K27me2
B.D.L.
0.01%
K14acR17me1K27me1K36me1
0.01%
B.D.L.
K9me2K14me1K36ac
B.D.L.
0.00%
K4me1K9me2K18acK36me3
B.D.L.
0.01%
R17me1K23acK27me1K36me1
0.01%
B.D.L.
K14me2R42me2
B.D.L.
0.00%
K36me1K37me1
0.00%
0.01%
K14acK18acK23acK27me3
0.01%
B.D.L.
R49me1
B.D.L.
0.00%
K9ac
0.00%
0.01%
K9me1K18acK23me2K27ac
0.01%
B.D.L.
K9me1R40me1R42me1
B.D.L.
0.00%
K9me1K14acR17me1K18acK23ac
B.D.L.
0.01%
K27me1R40me1
0.01%
B.D.L.
K9me2K23acK37me1
B.D.L.
0.00%
K18me1K23me1K27me1K36ac
B.D.L.
0.01%
K14acK18me2K23acK36me1
0.01%
B.D.L.
K4ac
B.D.L.
0.00%
R26me1K36me1K37me1
B.D.L.
0.01%
K4me1K18acK23acK27me2K36me2
0.01%
B.D.L.
K9me1K14acR17me1
B.D.L.
0.00%
K9me2K14acK18acK23acR40me2
B.D.L.
0.01%
K9me1K23acK27me2K36ac
0.01%
B.D.L.
K9me2K14me1K36me3
B.D.L.
0.00%
K37me2
B.D.L.
0.01%
K14acK18acK27me2K36me1K37me1
0.01%
B.D.L.
K18me1K23ac
B.D.L.
0.00%
K9me2K23acK27ac
0.01%
0.01%
K14me1K18acK27me2K36me2
0.01%
B.D.L.
K23acK27acK36me2
0.02%
0.02%
R8me1K14acK18me1K23me1K27ac
B.D.L.
0.01%
K14acK18acK23acK27me2K36me1
0.01%
B.D.L.
K18me2K23ac
B.D.L.
0.00%
K9me1K14me1K18me1K23me1K27acK36me2
0.00%
0.01%
K9me2K18acK23me3K27ac
0.01%
B.D.L.
K9me2R17me1
B.D.L.
0.00%
K4me1K9me1K14acK27me1K36me1K37me1
B.D.L.
0.01%
K27acR40me2
0.01%
B.D.L.
K18acK36me2
0.00%
0.00%
K18me1K23acR26me2
B.D.L.
0.01%
R8me1K14acK18acK23me1K27me1K36me1
0.01%
B.D.L.
K18acK23me1
B.D.L.
0.00%
K4me2K23acK27me1K36me3
B.D.L.
0.01%
K14acR26me2K27me1K36me1
0.01%
B.D.L.
K18me3K36ac
B.D.L.
0.00%
K9me2K18acK23acK36me3
B.D.L.
0.01%
K9me1K14me1K18acK23acK36me2
0.11%
0.10%
R17me2K23acK27me2K36me2
0.01%
0.01%
K9me2K27acR42me2
B.D.L.
0.01%
K14me2
B.D.L.
0.01%
K9me3K23me2R26me2K27me2
B.D.L.
0.03%
K23me1
0.01%
0.07%
K9me2K14me1R40me1R42me1
B.D.L.
0.01%
R8me1R17me1K27me1
B.D.L.
0.03%
K9me2K18acK36me1K37me1
B.D.L.
0.06%
K9me3K37me2
B.D.L.
0.01%
K4me1K9me1K14acR17me1K18acK23acK36me3
B.D.L.
0.03%
K4me1K18acK36me2
B.D.L.
0.06%
K9me2K23me1K27me3
B.D.L.
0.01%
K9me2K18ac
B.D.L.
0.03%
K4me1K9me1K18acK27me1K36me1K37me1
B.D.L.
0.06%
K9me2K27acK36me3
B.D.L.
0.01%
K14acK23me3
0.09%
0.11%
K9acK14me2K18acK27me1
B.D.L.
0.06%
K4me1K9me1K14me1K23acK27acK36me2
B.D.L.
0.01%
K23acK27me1K36me1
0.07%
0.09%
K9me1K14acK18ac
B.D.L.
0.06%
K9me2K14acK18acR49me2
B.D.L.
0.01%
K9me1K14me1K23acK27acK36me2
B.D.L.
0.03%
K9me2K18acK36me3
B.D.L.
0.06%
R17me1K27me3
B.D.L.
0.01%
K4me1R8me1K9me1R17me1K27me2K36me2
B.D.L.
0.03%
K18me3
0.03%
0.09%
K9me3K14ac
B.D.L.
0.01%
R26me1K36me2
0.02%
0.05%
K9me2K18acK23acK36me1K37me1
0.00%
0.07%
K9me2K18acR49me2
B.D.L.
0.01%
K9me1K14acK18acR40me2
B.D.L.
0.03%
R17me1
0.04%
0.11%
K14me2K27me1
B.D.L.
0.01%
K9me2K27acK36me1K37me1
0.01%
0.03%
K9me2K23me1
B.D.L.
0.07%
K9acK14me2K36me1
B.D.L.
0.01%
K4me2K14me1K23acK36me3
B.D.L.
0.03%
K9me2K18acK23acK27me1K36me1
B.D.L.
0.07%
K9me2R26me1K36me1R40me1
B.D.L.
0.01%
K9me2R26me1K27me1K36me1R40me1
B.D.L.
0.03%
R26me1
B.D.L.
0.07%
K27acK36me1
B.D.L.
0.01%
K9me2K18acK27me1K36me1
B.D.L.
0.03%
K23me2
0.00%
0.07%
K9me3K14me3K23acK36me2
B.D.L.
0.01%
K9me2K18acK23acR26me2
B.D.L.
0.03%
K9me2R26me1K36me1K37me1
B.D.L.
0.08%
K9me2K23acR26me2
B.D.L.
0.01%
K9acK36me2
B.D.L.
0.03%
K4me1K14acK36me2
B.D.L.
0.08%
R17me2K23acK27ac
B.D.L.
0.01%
R17me2K18acK27me2K36me2
0.04%
0.07%
K4me1K9me1K36me2
B.D.L.
0.08%
R17me2K18me1
B.D.L.
0.01%
K9me3R26me2K27me2K36me2
B.D.L.
0.03%
K18me1K23me1K27me1
B.D.L.
0.08%
24
Table S3 - continues
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
Histone code
Wild type Suz12-/-
K4me1K9me2K36me3
B.D.L.
0.01%
K9me2K14acK18acK23acK27me1K36me1
B.D.L.
0.03%
K9me2K23acK37me3
B.D.L.
0.08%
K4me1K9me3K27me2K36me2
B.D.L.
0.01%
R8me1K23acK27me2
B.D.L.
0.03%
K9me1K14me1K27acK36me2
B.D.L.
0.08%
K9me2K36me3K37me1
B.D.L.
0.02%
K9me3K36me1
B.D.L.
0.03%
R40me2
B.D.L.
0.08%
K9me2K23me1K27me1K36ac
B.D.L.
0.02%
K9me2K14acK18me1K23me1K27me1
B.D.L.
0.03%
R17me2K36me2
B.D.L.
0.08%
R17me1K36me1
B.D.L.
0.02%
K23acR26me1
B.D.L.
0.03%
K9me1K14me1K18me1K23acK36me2
B.D.L.
0.08%
K23me3K37me2
B.D.L.
0.02%
K9me1K14me1K23acR40me1
B.D.L.
0.03%
K9me1K14ac
B.D.L.
0.08%
K9acK14me2K18acK23acK36me3
B.D.L.
0.02%
K14me1K18me1K23acK36me2
B.D.L.
0.03%
K9acK14me2K27me1K36me1
B.D.L.
0.08%
K4me1K9me1R17me1K27me2K36me2
B.D.L.
0.02%
K9me2K23me1K27me1K36me1K37me1
B.D.L.
0.03%
K14acR17me1K18me1K23ac
B.D.L.
0.08%
K23acK27me1K36me1K37me1
0.02%
0.04%
K9me2K27me1K36me1R49me1
B.D.L.
0.03%
K14acK18acR26me1
0.01%
0.09%
K9me1K14acR42me1R49me1
B.D.L.
0.02%
K9me3K14me3K36me2
B.D.L.
0.03%
K9acK14acK18me1K23me1K27me1K36me2
B.D.L.
0.08%
R8me1K9me1K27me2
B.D.L.
0.02%
K14me2K23acK36me1
B.D.L.
0.03%
R17me1K23me1K27ac
B.D.L.
0.08%
K9me2R40me1
B.D.L.
0.02%
K9me2K36me1R42me1
B.D.L.
0.04%
K9me1K18me2
B.D.L.
0.08%
K9me3K23acK36me1K37me1
B.D.L.
0.02%
K4me1R8me1K9me1K14acK23acK27me1K36me1K37me1
B.D.L.
0.04%
K9me2K14acK23me3K27me3
B.D.L.
0.09%
K9me3K14acK23me2K27me2K36me2
0.19%
0.21%
R42me2
B.D.L.
0.04%
K14me2K36me1
B.D.L.
0.09%
K18me1K36me2
B.D.L.
0.02%
K9me2K18acK23me1K27ac
B.D.L.
0.04%
K9me3K27me2
B.D.L.
0.09%
K4me1K9me1K14me1K36me2
B.D.L.
0.02%
K9me2K18acR42me2
B.D.L.
0.04%
K4me1K9me1K27me2K36me2
B.D.L.
0.09%
K9acK14me1K18me1K23me1K27ac
B.D.L.
0.02%
K4me1K9me1K14me1K23acK36me2
B.D.L.
0.04%
K9me2K14acK18ac
B.D.L.
0.09%
K9acK14me3K18acK23acK27me2
B.D.L.
0.02%
K9me3K36ac
B.D.L.
0.04%
K9acK18me1K23me1K27me1K36me2
0.01%
0.10%
K9me1K23me2K36ac
B.D.L.
0.02%
K4me1R8me2K27me2K36me2
0.01%
0.05%
K9me2R17me1K36me1
B.D.L.
0.09%
K36ac
0.02%
0.04%
K9me2K27acK36me1
B.D.L.
0.04%
K9me2K27me1R49me2
0.02%
0.11%
K9me2K14me3
B.D.L.
0.02%
K9me2K14acK37me3
B.D.L.
0.04%
K4me1R8me1K9me1K18acK36me3
B.D.L.
0.09%
K9me2R26me1K36me3
B.D.L.
0.02%
K9me3K27me1K36me1K37me1
B.D.L.
0.04%
K4me1K9me1K18acK36me2
B.D.L.
0.09%
R17me1R26me2K36ac
B.D.L.
0.02%
K23acK36me1K37me1
0.01%
0.05%
K9me1R17me1K23ac
B.D.L.
0.09%
K9me1K14me1K18me1K36me2
0.03%
0.05%
K9me1K14acR40me2
B.D.L.
0.04%
K9me2K14acK18acK27me1
B.D.L.
0.09%
K9me2K37me1R40me1
B.D.L.
0.02%
K9me2K18me1
B.D.L.
0.04%
K9me2K14acK23me3
B.D.L.
0.09%
K9me2
B.D.L.
0.02%
K9me2K23me3K36ac
B.D.L.
0.04%
K9me2R26me1K27me1R40me1R42me1
0.02%
0.11%
K9me2K18acR40me2
B.D.L.
0.02%
K9me2K14me1K23me3K36ac
B.D.L.
0.04%
K4me1K9me1K18acR26me1K36me3
B.D.L.
0.10%
K23acR26me1R40me2
B.D.L.
0.02%
K9me2K14acK18acR42me2
B.D.L.
0.04%
K9me2R26me1K27me1K36me1K37me1
0.07%
0.17%
R49me2
0.00%
0.02%
R17me2K18acK23acK27me2K36me2
B.D.L.
0.04%
K9me2R49me2
B.D.L.
0.10%
K14acR26me1
B.D.L.
0.02%
K9me3K14acK18acK36me1K37me1
B.D.L.
0.04%
K9me1K23me2
B.D.L.
0.11%
K23me1K36me2
0.01%
0.03%
K9me1K14me1K23ac
B.D.L.
0.04%
K9me3K27me2K36me1
0.02%
0.13%
K9acK14me1R17me1K18acK23ac
B.D.L.
0.02%
K4me3K9me3K36me2
B.D.L.
0.04%
K9me2K27me1
B.D.L.
0.11%
K9me2K27me1R42me2
B.D.L.
0.02%
K9me1K14acK27me2
0.01%
0.06%
K36me1
0.26%
0.37%
K9me2K14ac
B.D.L.
0.02%
R8me2K36me2
B.D.L.
0.05%
K9acK14me1K18me1K23ac
B.D.L.
0.11%
K9me2R49me1
B.D.L.
0.02%
K9me2K23acR42me1
B.D.L.
0.05%
K4me1K9me2K14acK23acK27me1K36me1K37me1
B.D.L.
0.11%
K4me1K9me2K27me3K37me1
B.D.L.
0.02%
K14me1K23ac
B.D.L.
0.05%
K23acK36me3
0.05%
0.16%
K9me1K18ac
B.D.L.
0.02%
K5ac
0.05%
0.10%
K9me3K23acK27me1K36me1K37me1
B.D.L.
0.12%
K18acK23acK27me1
0.11%
0.13%
R17me1K36me2
B.D.L.
0.05%
K9me2K36me1R40me1
B.D.L.
0.12%
K9me1K14me1R26me1K36ac
B.D.L.
0.02%
K9me2K37me1R42me1
B.D.L.
0.05%
K9me2K18acK36me1
B.D.L.
0.12%
R8me2K36me1
B.D.L.
0.02%
K18acK23ac
0.05%
0.10%
K27ac
0.04%
0.16%
K9me2K18acK23me1K27me1K36me1K37me1
B.D.L.
0.02%
K4me1
B.D.L.
0.05%
K14me1K36me2
0.00%
0.12%
R8me2K27me2K36me2
0.09%
0.11%
K14me1
B.D.L.
0.05%
K9me1K14me1K18me1
B.D.L.
0.12%
K9me3K27me1K36ac
B.D.L.
0.02%
R8me2K14acK27me2K36me2
0.08%
0.13%
K9me2K23acK27acK36me1
B.D.L.
0.12%
K4me2K14acK18acK27me1K36me1K37me1
B.D.L.
0.03%
K9me1K23acR40me1
B.D.L.
0.05%
K9me2K27me1K36ac
B.D.L.
0.12%
K9me2K14me1
B.D.L.
0.03%
R8me1R17me1K36me1
B.D.L.
0.05%
K9me2K18acK23acK36me1
B.D.L.
0.12%
K9me2K27me1R40me1R42me1
B.D.L.
0.03%
K14acK18acK36me2
B.D.L.
0.05%
K9me1R17me2
B.D.L.
0.12%
K9me2K14acK23me3K27me1K36me1
B.D.L.
0.03%
K9acK14me2K18acK23ac
B.D.L.
0.05%
R8me1K9me2
B.D.L.
0.13%
K9me2K14acK18acK36me1
B.D.L.
0.03%
K9me3K36me1K37me1
B.D.L.
0.05%
K9me2K23acK27me1
B.D.L.
0.13%
K18me2
B.D.L.
0.03%
K4me1K18acK23acK36me2
B.D.L.
0.05%
K9me1K14me1R17me1
B.D.L.
0.13%
K4me1K9me1K14acK23acK27me1K36me1K37me1
B.D.L.
0.03%
K27acK36me2
0.07%
0.13%
K9me2K14acK23me1K27me1K36me1K37me1
B.D.L.
0.14%
K9me2K27me1K36me1R42me1
B.D.L.
0.03%
K14me2R17me1
B.D.L.
0.06%
K14me1K18me1K36me2
0.01%
0.15%
K9me2R40me1R42me1
B.D.L.
0.03%
K9me3K14acK27me1K36me1K37me1
B.D.L.
0.06%
K9acK14me1K18me1K36me2
B.D.L.
0.14%
K23acK27me1
0.42%
0.57%
K14me3
0.02%
0.34%
R8me1K9me1K27me2K36me2
3.98%
4.73%
K9acK14me3K18acK23acK27me1K36me1
B.D.L.
0.15%
K4me1K9me1K23acK36me2
B.D.L.
0.33%
K14acK18me2K23ac
B.D.L.
0.79%
R8me1R17me1K36me2
B.D.L.
0.15%
K9me2K23acR40me2
B.D.L.
0.33%
K9acK14me1K18me1K23acK36me2
0.04%
0.87%
K4me1K9me1K14me1K18acK36me2
B.D.L.
0.15%
K23acK36me1
0.04%
0.37%
K4me1K23acK36me2
B.D.L.
0.83%
K9me2K27me1K36me1R40me1
B.D.L.
0.16%
K4me1K9me2K14acK36me3
B.D.L.
0.33%
K9me2K14acR40me2
B.D.L.
0.91%
K9me1K23ac
B.D.L.
0.16%
K4me1K9me1K14acK36me2
B.D.L.
0.35%
K9me2R40me2
B.D.L.
0.94%
K18me1
0.01%
0.17%
K9me2K14acK18acK27me3
B.D.L.
0.36%
K4me1K9me1K14acK27me2K36me2
0.20%
1.14%
K14acK18acK23ac
0.01%
0.18%
K9me1K14acK18acK27me2
0.13%
0.49%
K9me2K14acK36me1K37me1
B.D.L.
0.95%
K27me1
0.35%
0.52%
K4me1K9me1K14acK18acK27me2K36me2
B.D.L.
0.38%
K9me2K23acK27me1K36me1K37me1
0.08%
1.05%
K9me2R17me1K36me1K37me1
B.D.L.
0.17%
K9me2R26me1K27me1K36me1
B.D.L.
0.39%
K9me2K23acK36me1
B.D.L.
1.00%
K36me3
0.01%
0.18%
K4me1K9me1K14acK18acK36me2
B.D.L.
0.40%
K9me2K14acK27me1K36me1
B.D.L.
1.13%
K9me2K23acR49me2
B.D.L.
0.18%
K9me1K23acK27me2
0.06%
0.47%
K9me2K23acK36me1K37me1
B.D.L.
1.14%
K9me2K14acR42me2
B.D.L.
0.18%
K9me2K23acR42me2
B.D.L.
0.43%
K9me2K27me1R40me2
0.06%
1.21%
K9me2K27me3
0.05%
0.23%
K14acK18ac
0.12%
0.57%
K23acK36me2
0.36%
1.75%
K9me2K14acK27me3K36me3
B.D.L.
0.19%
K9me2K14acK36me1
B.D.L.
0.48%
K9me2K36me1
B.D.L.
1.42%
K9me2K18acK23acK27me1
B.D.L.
0.19%
K9me2K14acK18acR40me2
B.D.L.
0.48%
K9me2K23acK27me1K36me1
B.D.L.
1.61%
K9me1
B.D.L.
0.19%
K9me1K14me2
B.D.L.
0.48%
K9me3K36me2
0.05%
1.69%
K9me2K14acK18acK36me3
0.03%
0.23%
K9me1K27me2
B.D.L.
0.50%
K9me2K23acK36me3
0.01%
1.67%
K4me1K9me1K14me1K27me2K36me2
0.06%
0.27%
K27me2
0.64%
1.18%
K9me2K36me1K37me1
B.D.L.
1.78%
R8me1K36me2
B.D.L.
0.21%
K9me2K14acK18acK27me1K36me1
0.04%
0.58%
K36me2
1.05%
2.93%
K9me2K36ac
B.D.L.
0.23%
K9me2R42me2
B.D.L.
0.54%
K9me1K14me1K23acK36me2
0.01%
2.09%
K9me1K23acR40me2
B.D.L.
0.24%
K9me2K18acK27me1K36me1K37me1
0.02%
0.60%
K9me3
0.09%
2.19%
K9me2K14acK18acK23acK36me1
B.D.L.
0.25%
K9me2K14acK18acK27me1K36me1K37me1
0.02%
0.60%
K9me2K36me3
0.22%
2.68%
K9me2K14acK18me1K23acK27ac
B.D.L.
0.26%
K9me2K14acK27me1K36me1K37me1
0.07%
0.67%
K9me2K27me1K36me1K37me1
0.26%
2.76%
K9me1K14me1K18acK36me2
B.D.L.
0.27%
K9me2K14acK36me3
B.D.L.
0.63%
K9me2K27me1K36me1
B.D.L.
2.70%
K18ac
0.03%
0.31%
K14ac
0.06%
0.70%
K9me1K36me2
0.00%
3.80%
R8me1K23acK27me1K36me1
B.D.L.
0.28%
K14acK18me1K23ac
0.04%
0.70%
K9me1K14me1K36me2
0.08%
9.01%
K9me2K14acR49me2
B.D.L.
0.30%
K23ac
0.71%
1.45%
25
Table S4
Wild type
Suz12-/-
Wild type
Suz12-/-
Wild type
Suz12-/-
K4me1K27me2K36me2
Histone code
1.30%
B.D.L.
R17me1K27me2K36me2
Histone code
0.16%
B.D.L.
K9me1K14me2
B.D.L.
0.34%
K9me1K14me1K27me3K36me2
1.52%
B.D.L.
K9me2K23acK27acK36me1
0.15%
B.D.L.
K9me2K18acK27me1K36me1
B.D.L.
0.82%
K9me1K14me1K27me2K36me2
8.54%
B.D.L.
K23acK36me1
0.15%
0.16%
K23me2
B.D.L.
0.76%
K9me1K27me2K36me2
11.24%
0.90%
K23ac
0.25%
1.46%
R40me1R42me2
B.D.L.
0.34%
K14me1K27me2K36me2
2.45%
B.D.L.
K9me1K14me1K18me1K23acK27me2K36me2
0.15%
B.D.L.
R8me2K18acK27me2K36me2
B.D.L.
0.33%
K27me2K36me2
10.25%
0.75%
K9me3K14acK27me2K36me2
0.27%
0.18%
K23acR42me1
B.D.L.
0.33%
K9me1R17me1R26me1K27me2K36me2
0.80%
B.D.L.
K14acK18me1K23acK27me3
0.14%
B.D.L.
K37me3
B.D.L.
0.33%
K27me3K36me1
2.78%
B.D.L.
K9me2K23acK27acR49me2
0.13%
B.D.L.
K9me2K23acK36me1
B.D.L.
1.21%
K9me1K23acK27me2K36me2
5.81%
B.D.L.
K9me1K14acK27me3
0.13%
B.D.L.
K23acK36me2
B.D.L.
0.52%
K27me2K36me1
5.66%
B.D.L.
K14acK23me3
0.12%
0.35%
K9me2K27me1
B.D.L.
0.52%
K9me1K18acK23acK27me2K36me2
0.67%
B.D.L.
K23acK27me1
0.30%
0.12%
K18ac
B.D.L.
0.62%
K9me3K27me2K36me2
7.00%
1.99%
K27me3R49me1
0.11%
B.D.L.
K18me2
B.D.L.
1.23%
K18acK27me2K36me2
0.69%
B.D.L.
K27me2K36me1K37me1
0.23%
B.D.L.
K9me2K14acR49me2
B.D.L.
0.29%
K9me2K27me3
1.23%
0.11%
K9me2K23acK27acR49me1
0.10%
B.D.L.
K9me1K14me1K18acK27me2K36me2
B.D.L.
0.29%
K9me1K14me1K18me1K36me2
0.62%
0.37%
K23acK36me1K37me1
0.10%
B.D.L.
R8me1K9me2
B.D.L.
0.28%
K9me1K27me2K36me3
0.62%
B.D.L.
K14acK18me2K23acK36me1K37me1
0.09%
B.D.L.
K4me1K9me3K36me2
B.D.L.
0.28%
K27me3
1.09%
0.55%
R26me1K36me2
0.08%
B.D.L.
K9me2K27me1R40me2
B.D.L.
0.46%
K9me1K14me1K36me2
0.61%
9.35%
K23acK37me2
0.08%
0.25%
R42me2R49me2
B.D.L.
0.27%
K9me2K27me3R49me2
0.57%
B.D.L.
K9me2R26me1K27me1K36me1
0.12%
0.30%
K14me1K23acK27me2
B.D.L.
0.27%
K9me1K14me1K23acK27me2K36me2
2.06%
B.D.L.
K27me1K36me1
0.13%
B.D.L.
K9me1K14me1K23acK27acK36me2
B.D.L.
0.26%
K9me1K14me1K23acK36me3
0.53%
0.04%
K27me2K37me1
0.07%
B.D.L.
K4me1K23acK36me2
B.D.L.
0.46%
K9me2K23acK27ac
0.50%
B.D.L.
K9me3K23me1K27me2K36me2
0.06%
B.D.L.
K9me2K23ac
B.D.L.
0.26%
K9me1K14me1K23acK36me2
0.49%
2.73%
K9me3K27me2K36me1K37me1
0.05%
B.D.L.
K4me2K23acK27me1K37me2
B.D.L.
0.25%
K27me2K36me3
0.47%
B.D.L.
K14me2K23acK27ac
0.05%
B.D.L.
K27me1R42me2
B.D.L.
0.24%
K27me3K36me3
0.47%
B.D.L.
K36me2
0.08%
3.17%
K14me2K23acK27me1
B.D.L.
0.24%
K27me1K36me2
1.50%
B.D.L.
K27ac
0.04%
B.D.L.
K18acK23acK37me1
B.D.L.
0.24%
K27me3K36me2
1.84%
B.D.L.
K18acK27me2
0.04%
0.28%
K9me2K14me3K23ac
B.D.L.
0.23%
R8me1R17me1K23acK27me2K36me2
0.46%
B.D.L.
K9acR17me2K27me2K36me2
0.04%
B.D.L.
K9me2R17me2K18ac
B.D.L.
0.22%
K9me1K23acK36me3
0.67%
B.D.L.
K9me3
0.04%
1.73%
K9me1K14acK23me3
B.D.L.
0.21%
R26me1K27me1K36me1
0.44%
B.D.L.
K23acK27me1K36me1
0.07%
B.D.L.
K9me2K18ac
B.D.L.
0.20%
K23acK27me2
1.25%
0.29%
K36me1
0.04%
1.69%
K9me2K14acK18acK36me1K37me1
B.D.L.
0.20%
R26me2K27me2K36me2
0.41%
B.D.L.
R26me2K36me2
0.03%
B.D.L.
K9acK14me2K27me1K36me1K37me1
B.D.L.
0.46%
K9me1R17me1K27me2K36me2
0.49%
B.D.L.
R8me1K18acK27me2K36me2
0.03%
B.D.L.
K9me2K23acK27me1K36me1K37me1
B.D.L.
0.31%
K9me2K36me3
1.03%
2.02%
R42me1
0.03%
B.D.L.
K9me3R49me1
B.D.L.
0.18%
K9me1K23acK27me3
0.39%
0.12%
K9me1K36me2
0.03%
9.53%
K14me2
B.D.L.
0.25%
R8me1K9me1K27me2K36me2
0.39%
0.36%
K14me3
0.01%
B.D.L.
K9me2K23acK27me1
B.D.L.
0.16%
K23acK27me2K36me1
1.96%
B.D.L.
K9me2K36me1K37me1
B.D.L.
2.94%
K4me2K18acK27me1K37me2
B.D.L.
0.16%
K23acK27me2K36me2
1.94%
B.D.L.
R17me2K36me2
B.D.L.
0.75%
K9me1
B.D.L.
0.32%
K9me2K23acK27me3
0.37%
B.D.L.
K9me2K23me1R49me2
B.D.L.
0.73%
R49me2
B.D.L.
0.15%
K9me1R26me1K36me3
0.36%
B.D.L.
K4me3K9me3K23acK36me2
B.D.L.
0.71%
R26me1
B.D.L.
0.15%
K36me2R42me2
0.36%
B.D.L.
R8me2K36me2
B.D.L.
0.63%
K9me3K36ac
B.D.L.
0.14%
K9me3R26me1K27me2K36me2
1.18%
B.D.L.
K14me1K36me2
B.D.L.
0.95%
K18me1K36me2
B.D.L.
0.14%
K9me3K27me2K36me1
1.32%
0.20%
K9me2K27me1K36me1
B.D.L.
4.54%
R17me2
B.D.L.
0.22%
R26me1K27me2K36me2
0.34%
B.D.L.
K36me3
B.D.L.
1.02%
K9me2K23acR42me2
B.D.L.
0.13%
K27me1K36me3
0.63%
B.D.L.
K9me2K27me1K36me1K37me1
B.D.L.
0.69%
R40me2
B.D.L.
0.13%
K9acK14me1K18me1K23acK27me2K36me2
0.32%
B.D.L.
K9me2K36me1
B.D.L.
1.91%
K18acK36me2
B.D.L.
0.12%
K23acK27me3
0.50%
B.D.L.
K9me2K18acK27me1K36me1K37me3
B.D.L.
0.56%
R17me2K23acK36me1
B.D.L.
0.12%
R17me2K27me2K36me2
0.32%
B.D.L.
K9me2K27me3K36me1
B.D.L.
0.54%
K9me2K18acK36me1
B.D.L.
0.12%
R26me2K27me2
0.56%
B.D.L.
K9me1K23acK27me2
B.D.L.
0.53%
R17me1K23ac
B.D.L.
0.12%
K9me1K14me1K18me1K23me1K27me1K36me2
0.29%
B.D.L.
K4me1K9me1K36me2
B.D.L.
0.52%
R26me2
B.D.L.
0.12%
K27me1
1.02%
2.04%
K9me2K14acK27me1K36me1
B.D.L.
0.68%
K9me2K27me1R49me2
B.D.L.
0.11%
K23acK27me2K36me1K37me1
0.36%
B.D.L.
K9me3K36me2
B.D.L.
2.16%
K18me1
B.D.L.
0.21%
K18acK23acK27me2
0.44%
0.36%
K9me2K14acK36me3K37me3
B.D.L.
0.50%
K9me2K36me1R49me2
B.D.L.
0.11%
K9acK18me1K23me1K36me2
0.27%
B.D.L.
K9acK14me1K18me1K23acK36me2
B.D.L.
0.83%
K23me3
B.D.L.
0.10%
K27me2
2.06%
0.95%
K14me2K36me1
B.D.L.
0.48%
K18acK27me1
B.D.L.
0.10%
K27me2R49me2
0.24%
B.D.L.
K9me2K23acK36me3
B.D.L.
1.23%
K14me1K23acK27me3
B.D.L.
0.09%
K9me2K27me3K36me1K37me1
0.24%
B.D.L.
K9me2K27me1K36me3
B.D.L.
0.78%
K23acR26me2
B.D.L.
0.09%
K14acK18me1K27me2K36me2
0.23%
B.D.L.
K9me2K14acK27me1K36me1K37me1
B.D.L.
0.48%
K18acR26me2
B.D.L.
0.09%
K9me2K14acK27me3
0.22%
B.D.L.
K9me2K23acK27me1K36me1
B.D.L.
1.36%
K4me1K23acK36me1K37me1
B.D.L.
0.08%
R8me1K9me1K14me1K27me2K36me2
0.21%
0.21%
K9me2
B.D.L.
0.69%
K14me1
B.D.L.
0.16%
K23acR26me1K27me1K36me1
0.21%
B.D.L.
K9me2K14acK18ac
B.D.L.
0.45%
K9me2R26me1K27me1
B.D.L.
0.08%
K18acK23acK27me1
0.20%
B.D.L.
R8me1K9me2K37me1
B.D.L.
0.76%
K14me3K27me2K36me2
B.D.L.
0.08%
K14me3K18me3K23acK27me2K36me2
0.19%
B.D.L.
K27me2R42me2
B.D.L.
0.44%
K9me2R17me1K36me1K37me1
B.D.L.
0.08%
K14me1K23acK27me2K36me2
0.30%
B.D.L.
K9me1K14me1K18acK23acK36me2
B.D.L.
0.44%
K9me2K18acK27me1K36me1K37me1
B.D.L.
0.07%
K9me3K14me3K23acK27me2K36me2
0.18%
B.D.L.
R8me2K18acK36me3
B.D.L.
0.43%
R26me1K27me1
B.D.L.
0.07%
(unmodified)
1.49%
7.15%
K23me1
B.D.L.
0.72%
K9me3K14me1
B.D.L.
0.06%
K9me1K18acK27me2K36me2
0.34%
B.D.L.
K9me2K14me1
B.D.L.
0.41%
R8me1K9me1K18ac
B.D.L.
0.06%
K9acK14me3K27me2K36me2
0.24%
0.18%
K9me1K14me1K18acK36me2
B.D.L.
0.97%
R8me1R17me1K27me1
B.D.L.
0.04%
R17me1R26me1K36me2
0.17%
B.D.L.
K9me2K23acK36me1K37me1
B.D.L.
0.40%
K14me2K23acK36me1
B.D.L.
0.04%
K9me3K23acK27me2K36me2
0.27%
B.D.L.
K36me1R42me2
B.D.L.
0.38%
K9acK14me2K27me1K36me1
B.D.L.
0.01%
K9acK18me1K27me2K36me2
0.16%
B.D.L.
R8me2K14acK27me2K36me2
B.D.L.
0.38%
K9me3K36me1K37me1
B.D.L.
0.01%
K36me1K37me2
B.D.L.
0.38%
26
Histone code
Table S5
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
K27me2K36me2
Binary co-existence
70.27%
11.84%
2.64E-11
K27me1K37me3
Binary co-existence
0.16%
0.00%
1.73E-02
K18me3K36me2
0.03%
0.01%
8.76E-02
K14me1K27me2
20.69%
1.81%
1.09E-09
K36me1K37me3
0.16%
0.00%
1.74E-02
K9acK27me1
0.02%
0.30%
8.95E-02
K9me2K27me1
1.74%
16.61%
1.16E-09
K9acK27me2
3.25%
0.25%
1.76E-02
K9acK27me3
0.09%
0.00%
9.02E-02
K27me1K36me1
2.18%
14.65%
1.35E-08
K9me3K37me2
0.00%
0.02%
1.81E-02
R8me1K9ac
0.03%
0.00%
9.21E-02
K9me2K36me1
3.22%
22.11%
4.41E-08
R42me2R49me2
0.18%
0.03%
1.81E-02
R8me1R26me1
0.03%
0.00%
9.21E-02
K27me2K36me1
3.89%
0.34%
8.37E-08
K27me3R40me1
0.23%
0.00%
1.87E-02
K23acK37me2
0.02%
0.00%
9.30E-02
K9me1K27me2
37.27%
10.11%
1.09E-07
K23acK36me3
0.98%
2.24%
1.88E-02
R26me1K37me1
0.14%
0.43%
9.39E-02
K27me1K37me1
1.03%
6.78%
2.50E-07
K9me2K14me1
0.00%
0.08%
1.91E-02
K37me1R42me1
0.00%
0.04%
9.40E-02
K9me2K37me1
1.85%
10.90%
2.14E-06
R26me2K27me1
0.14%
0.00%
2.02E-02
K23me3K27ac
0.29%
0.00%
9.47E-02
R26me1K27me2
1.03%
0.01%
2.75E-06
K14me1K23ac
6.88%
3.63%
2.03E-02
K18acK23me2
0.05%
0.00%
9.54E-02
K36me1K37me1
2.92%
11.58%
4.46E-06
K36me1R40me1
0.00%
0.46%
2.23E-02
R8me1K27me3
0.07%
0.00%
9.77E-02
R26me1K36me2
1.13%
0.05%
6.80E-06
K9me1K18me1
0.04%
0.24%
2.24E-02
R8me2K27me2
0.75%
0.46%
9.83E-02
K9me2K23ac
0.80%
9.47%
1.44E-05
K14me1K23me2
0.03%
0.00%
2.46E-02
K9me1K18ac
3.15%
2.19%
9.86E-02
K9me2R40me2
0.34%
4.43%
1.57E-05
R8me1K36me1
0.07%
0.35%
2.57E-02
K27me1R40me1
0.03%
0.58%
1.04E-01
K4me1K36me2
1.38%
4.46%
1.82E-04
K18me1K23me2
0.25%
0.00%
2.71E-02
K23me1K36me1
0.03%
0.18%
1.05E-01
K14me1K27me3
0.24%
0.00%
2.11E-04
K27me2R40me1
0.04%
0.00%
2.79E-02
K23acK37me3
0.24%
0.10%
1.05E-01
K9me2K18ac
0.47%
4.42%
2.19E-04
K23me3K36ac
0.00%
0.09%
2.86E-02
K27acR40me2
0.02%
0.00%
1.06E-01
K23acK27me2
23.19%
1.51%
2.52E-04
K18me2K23me2
0.04%
0.00%
3.01E-02
K14acK23me3
1.06%
0.53%
1.09E-01
K9me3K27me2
10.81%
2.82%
2.81E-04
K9me1K14me1
17.87%
14.08%
3.08E-02
K9acK18me2
0.01%
0.00%
1.10E-01
K9me2K36me3
0.94%
7.12%
2.85E-04
K9me2K23me1
0.10%
0.36%
3.21E-02
R8me1K18ac
0.16%
0.05%
1.12E-01
K27me2K36me3
0.37%
0.00%
3.69E-04
K14me3K27me2
0.17%
0.01%
3.22E-02
K18acK37me3
0.08%
0.00%
1.12E-01
K14me1K36me2
21.22%
14.74%
4.01E-04
K4me1K36me3
0.00%
0.40%
3.26E-02
K9acK14me1
2.39%
0.95%
1.13E-01
K9me2K14ac
0.50%
9.19%
4.06E-04
K9acK23me2
0.18%
0.00%
3.39E-02
K27me2K37me3
0.05%
0.00%
1.13E-01
K9me2R42me2
0.18%
1.13%
4.66E-04
K9acR26me1
0.06%
0.00%
3.58E-02
K9me3K18me2
0.02%
0.00%
1.15E-01
K18acK27me2
8.76%
1.25%
5.11E-04
K23me2K27ac
0.19%
0.00%
3.64E-02
K9acK36me3
0.00%
0.04%
1.17E-01
K9me1K36me2
37.38%
24.94%
5.43E-04
R8me1K27me1
0.07%
0.32%
3.66E-02
K18me2K27me2
0.02%
0.00%
1.17E-01
K18acK36me2
7.06%
1.69%
5.73E-04
K18acK36me3
0.15%
0.52%
3.71E-02
K18me2K36me2
0.02%
0.00%
1.17E-01
K14acK37me1
0.64%
2.64%
6.01E-04
K9me3K36ac
0.01%
0.10%
3.72E-02
R8me2K14ac
0.34%
0.09%
1.17E-01
K14acK36me1
1.01%
5.19%
6.97E-04
K18acK23me3
0.03%
0.00%
3.79E-02
K27me1R42me1
0.01%
0.31%
1.19E-01
K27me1R40me2
0.06%
1.34%
8.28E-04
K23me3K36me2
0.38%
0.02%
3.80E-02
R26me1R40me1
0.02%
0.30%
1.20E-01
K9me3K23ac
1.37%
0.13%
8.89E-04
K18me2K37me1
0.04%
0.00%
3.90E-02
K23acR40me1
0.00%
0.06%
1.20E-01
K14acK27me1
1.27%
4.00%
9.15E-04
K4me1K23ac
1.08%
1.74%
3.90E-02
K18me2K36me3
0.03%
0.00%
1.20E-01
R17me1K27me2
1.19%
0.03%
1.05E-03
K9me1R17me2
0.00%
0.17%
4.05E-02
K18me2K23me1
0.04%
0.00%
1.20E-01
K27me3K37me2
0.02%
0.00%
1.16E-03
K27me2R49me2
0.04%
0.00%
4.07E-02
K18me2K27ac
0.04%
0.00%
1.20E-01
K27me3K36me1
2.60%
0.08%
1.26E-03
K23me1K27me2
0.03%
0.00%
4.15E-02
K18me2K27me3
0.07%
0.00%
1.20E-01
K9me3K36me2
10.70%
4.15%
1.28E-03
K27me2R40me2
0.08%
0.00%
4.23E-02
R8me2K36me2
0.75%
0.49%
1.21E-01
K27me3K36me2
1.34%
0.00%
1.35E-03
K9me2K36ac
0.13%
0.55%
4.26E-02
K4me1R8me2
0.01%
0.06%
1.21E-01
K23acK27me1
1.79%
4.49%
1.57E-03
K9me3K36me1
0.19%
0.55%
4.27E-02
R17me1K23ac
0.13%
0.32%
1.22E-01
K23acK36me2
19.02%
6.72%
1.64E-03
K14me2R42me2
0.04%
0.00%
4.29E-02
K9me2R40me1
0.24%
0.76%
1.22E-01
K23acK37me1
1.00%
2.74%
1.67E-03
K9me1R17me1
0.73%
0.33%
4.42E-02
K9me1K36me3
0.65%
0.19%
1.23E-01
K23acR42me2
0.19%
0.48%
1.73E-03
R8me1R17me1
0.50%
0.18%
4.44E-02
R17me1K23me2
0.06%
0.00%
1.24E-01
K9me3K14ac
3.13%
1.05%
1.79E-03
K9me3K27me1
0.03%
0.29%
4.49E-02
K27me3R49me2
0.06%
0.00%
1.24E-01
K4me1K18ac
0.26%
1.34%
1.97E-03
R17me2K23ac
0.01%
0.06%
4.55E-02
K14me1R42me1
0.00%
0.01%
1.25E-01
K4me1K9me1
1.26%
3.61%
2.01E-03
K18me2K27me1
0.06%
0.00%
4.55E-02
K4me1K27me1
0.10%
0.40%
1.26E-01
K4me1K14ac
0.62%
2.93%
2.03E-03
K14acK23me2
0.73%
0.15%
4.70E-02
R17me1R26me1
0.20%
0.00%
1.26E-01
K27me3K37me1
1.20%
0.07%
2.13E-03
K18me2K23ac
0.21%
0.92%
4.80E-02
K27me1R49me2
0.03%
0.17%
1.26E-01
K9me1K27me3
0.74%
0.00%
2.13E-03
R8me1K23me2
0.13%
0.00%
4.84E-02
K23me3K37me1
0.17%
0.00%
1.27E-01
K9me3K18ac
1.58%
0.10%
2.20E-03
K9me1K18me2
0.00%
0.10%
4.84E-02
K9me3K27ac
0.05%
0.00%
1.28E-01
K14me1K18ac
1.11%
0.52%
2.21E-03
K27me2R42me1
0.03%
0.00%
5.05E-02
K4me1K9me2
0.18%
0.44%
1.29E-01
K14acK27me2
12.63%
3.24%
2.24E-03
K18acK23ac
2.91%
1.58%
5.17E-02
K9acR17me1
0.16%
0.01%
1.31E-01
K9me1K23ac
9.99%
4.53%
2.56E-03
R26me2K36me3
0.03%
0.00%
5.37E-02
K18acR40me2
0.08%
0.55%
1.32E-01
K27me1K36me2
1.59%
0.35%
2.64E-03
K14acK36me3
0.41%
1.23%
5.38E-02
K14me3K27me1
0.00%
0.11%
1.32E-01
K9me1R40me2
0.00%
0.40%
2.79E-03
K23me1K36me3
0.11%
0.01%
5.59E-02
K14me3R40me1
0.00%
0.00%
1.32E-01
K18acK37me1
0.19%
1.27%
2.89E-03
K9me2K27me3
2.81%
1.29%
5.62E-02
K9acK23ac
1.84%
0.92%
1.34E-01
K27me3R40me2
0.19%
0.00%
3.60E-03
K18me1K36me3
0.13%
0.01%
5.97E-02
K9acK23me3
0.14%
0.00%
1.36E-01
K9me2R26me1
0.06%
1.00%
3.72E-03
K9me1K14me2
0.01%
0.49%
6.26E-02
K23me3K36me1
0.28%
0.07%
1.36E-01
K14me2K27ac
0.05%
0.00%
4.06E-03
K9me1K23me2
0.32%
0.13%
6.27E-02
K27acK37me1
0.11%
0.04%
1.36E-01
K14acK36me2
9.96%
3.50%
4.13E-03
K23me1K37me1
0.00%
0.18%
6.38E-02
K9me3K37me1
0.23%
0.44%
1.37E-01
K18acK27me1
0.90%
2.54%
5.16E-03
K14acK18me2
0.26%
0.92%
6.41E-02
R26me1R42me1
0.01%
0.21%
1.37E-01
K18acK36me1
0.54%
2.56%
5.26E-03
K27me2K36ac
0.04%
0.01%
6.57E-02
K14acR26me2
0.04%
0.01%
1.38E-01
K27me2K37me1
0.69%
0.12%
6.67E-03
R26me2K37me1
0.06%
0.00%
6.64E-02
K36me2K37me1
0.08%
0.01%
1.40E-01
R17me1K36me2
1.27%
0.23%
6.69E-03
K9me2R49me2
0.37%
1.03%
6.76E-02
K18me2K36ac
0.02%
0.00%
1.40E-01
K23me1K36ac
0.00%
0.05%
7.24E-03
K14acR49me2
0.04%
0.36%
6.84E-02
K23acR42me1
0.01%
0.06%
1.42E-01
K14me1K27ac
0.00%
0.16%
7.95E-03
K4me1K14me1
0.19%
0.44%
6.86E-02
R17me1K23me1
0.00%
0.07%
1.43E-01
K14acR40me2
0.01%
1.42%
8.02E-03
K18me1K36me2
2.69%
1.22%
7.09E-02
K27me2R42me2
0.01%
0.00%
1.44E-01
K18me1K27me2
3.14%
0.00%
8.15E-03
K9acK36me2
3.34%
1.23%
7.10E-02
K9acK18me1
2.08%
1.06%
1.44E-01
K23acR26me1
0.98%
0.10%
8.33E-03
K14me1K36ac
0.00%
0.05%
7.28E-02
R17me1K36me1
0.12%
0.28%
1.45E-01
K4me1K27me3
0.55%
0.02%
8.46E-03
R17me1K18me1
0.00%
0.17%
7.39E-02
K9acR42me2
0.02%
0.00%
1.45E-01
K23acR40me2
0.04%
0.97%
8.79E-03
K27me3R42me1
0.13%
0.00%
7.74E-02
K18acR26me2
0.10%
0.02%
1.48E-01
K23acK36me1
3.34%
6.65%
9.06E-03
K23me2K27me3
0.06%
0.00%
7.85E-02
K9me2K18me1
0.07%
0.34%
1.49E-01
K18me2K36me1
0.10%
0.00%
9.73E-03
R26me2K36me2
0.17%
0.02%
7.86E-02
K9me1R42me2
0.11%
0.00%
1.49E-01
K23me2R26me1
0.08%
0.00%
9.95E-03
R17me1R26me2
0.00%
0.03%
7.87E-02
R26me1R42me2
0.11%
0.00%
1.49E-01
R8me1K14me1
0.54%
0.03%
1.06E-02
K9me1K27ac
0.03%
0.11%
8.02E-02
K14me1K23me3
0.00%
0.03%
1.50E-01
R26me2K36me1
0.15%
0.01%
1.18E-02
K9me1R26me2
0.00%
0.01%
8.11E-02
K27acR42me1
0.01%
0.00%
1.50E-01
K9me2R17me1
0.00%
0.24%
1.27E-02
R8me2K18ac
0.31%
0.05%
8.36E-02
K4me1K14me3
0.00%
0.00%
1.51E-01
K23me1K36me2
0.78%
0.16%
1.33E-02
K14me3R42me1
0.00%
0.00%
8.40E-02
R8me2K14me3
0.00%
0.00%
1.51E-01
K23me2K36me2
1.03%
0.15%
1.34E-02
K23me3K27me3
0.50%
0.09%
8.53E-02
R8me1K14me3
0.00%
0.00%
1.51E-01
K23acK27me3
0.92%
0.06%
1.44E-02
K14me2K36me1
0.00%
0.21%
8.56E-02
K9me1K14me3
0.00%
0.00%
1.51E-01
K23me2K27me2
1.03%
0.18%
1.67E-02
K36me1R42me1
0.00%
0.11%
8.59E-02
K14me3R17me1
0.00%
0.00%
1.51E-01
K23me3K27me2
0.51%
0.00%
1.68E-02
R17me2K18me1
0.00%
0.03%
8.74E-02
K14me3R17me2
0.00%
0.00%
1.51E-01
27
Binary co-existence
Table S5 - continues
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
K14me3K18me1
Binary co-existence
0.00%
0.00%
1.51E-01
K9me3K14me2
Binary co-existence
0.00%
0.00%
1.90E-01
K36me3K37me3
0.00%
0.00%
2.90E-01
K14me3K23me1
0.00%
0.00%
1.51E-01
K14me2R17me2
0.00%
0.00%
1.90E-01
K37me3R40me2
0.00%
0.00%
2.90E-01
K14me3K23me2
0.00%
0.00%
1.51E-01
K14me2K18me1
0.00%
0.00%
1.90E-01
K37me3R49me2
0.00%
0.00%
2.90E-01
K14me3R26me2
0.00%
0.00%
1.51E-01
K14me2K23me1
0.00%
0.00%
1.90E-01
R17me2K18ac
0.05%
0.08%
2.90E-01
K14me3R26me1
0.00%
0.00%
1.51E-01
K14me2K23me2
0.00%
0.00%
1.90E-01
K18acR49me2
0.04%
0.07%
2.93E-01
K14me3K27me3
0.00%
0.00%
1.51E-01
K14me2R26me1
0.00%
0.00%
1.90E-01
K18acK23me1
0.04%
0.08%
2.95E-01
K14me3K27ac
0.00%
0.00%
1.51E-01
K14me2R26me2
0.00%
0.00%
1.90E-01
K9me2K14me2
0.00%
0.01%
2.96E-01
K14me3K36me3
0.00%
0.00%
1.51E-01
K14me2K27me3
0.00%
0.00%
1.90E-01
K14me2K36me3
0.00%
0.03%
2.99E-01
K14me3R40me2
0.00%
0.00%
1.51E-01
K14me2K27me2
0.00%
0.00%
1.90E-01
K4me1K18me3
0.00%
0.00%
2.99E-01
K14me3R49me2
0.00%
0.00%
1.51E-01
K14me2K36me2
0.00%
0.00%
1.90E-01
R8me1K18me3
0.00%
0.00%
2.99E-01
R26me1K27me3
0.10%
0.00%
1.51E-01
K14me2K37me1
0.00%
0.00%
1.90E-01
R8me2K18me3
0.00%
0.00%
2.99E-01
K14me3K36me1
0.01%
0.11%
1.52E-01
K14me2R40me2
0.00%
0.00%
1.90E-01
K9me1K18me3
0.00%
0.00%
2.99E-01
K4me1K9ac
0.12%
0.00%
1.54E-01
K14me2R42me1
0.00%
0.00%
1.90E-01
K9me2K18me3
0.00%
0.00%
2.99E-01
R26me2K27ac
0.00%
0.00%
1.54E-01
K14me2R49me2
0.00%
0.00%
1.90E-01
K14acK18me3
0.00%
0.00%
2.99E-01
R17me1K36me3
0.00%
0.05%
1.55E-01
K9acR49me2
0.00%
0.00%
1.92E-01
R17me1K18me3
0.00%
0.00%
2.99E-01
K9me2R42me1
0.14%
0.47%
1.56E-01
K14me1K18me1
2.16%
1.30%
1.93E-01
R17me2K18me3
0.00%
0.00%
2.99E-01
R17me1K27ac
0.00%
0.06%
1.60E-01
K23me3R26me1
0.09%
0.00%
1.94E-01
K18me3K23ac
0.00%
0.00%
2.99E-01
K14me1R40me1
0.00%
0.04%
1.60E-01
K14acR42me1
0.00%
0.03%
1.94E-01
K18me3K23me1
0.00%
0.00%
2.99E-01
K14me3K23ac
0.06%
0.17%
1.62E-01
K9me1R26me1
0.36%
0.10%
1.97E-01
K18me3K23me2
0.00%
0.00%
2.99E-01
K4me1K27me2
1.41%
1.92%
1.63E-01
K27acK36me3
0.06%
0.01%
1.99E-01
K18me3R26me1
0.00%
0.00%
2.99E-01
K9me3R26me1
0.03%
0.01%
1.63E-01
K14me2R17me1
0.00%
0.06%
2.01E-01
K18me3R26me2
0.00%
0.00%
2.99E-01
K14me1R26me1
0.05%
0.02%
1.63E-01
K9me1K36me1
0.31%
0.19%
2.02E-01
K18me3K27ac
0.00%
0.00%
2.99E-01
K14me1K36me3
0.00%
0.05%
1.64E-01
K14me3R42me2
0.00%
0.00%
2.06E-01
K18me3K27me1
0.00%
0.00%
2.99E-01
K9me2K14me3
0.00%
0.02%
1.64E-01
R8me1K36me3
0.01%
0.05%
2.06E-01
K18me3K27me3
0.00%
0.00%
2.99E-01
K14me2K18me3
0.00%
0.00%
1.65E-01
K9me3K23me2
0.34%
0.17%
2.08E-01
K18me3K36me1
0.00%
0.00%
2.99E-01
K4me1K9me3
0.00%
0.01%
1.65E-01
R17me1K27me3
0.05%
0.01%
2.10E-01
K18me3K36me3
0.00%
0.00%
2.99E-01
K36me3K37me1
0.00%
0.01%
1.65E-01
R26me2R40me2
0.12%
0.05%
2.11E-01
K18me3K37me1
0.00%
0.00%
2.99E-01
K27me1K36ac
0.18%
0.33%
1.66E-01
K14me2K27me1
0.00%
0.11%
2.13E-01
K18me3R40me2
0.00%
0.00%
2.99E-01
R17me2K27ac
0.00%
0.01%
1.67E-01
K9acK14me2
0.04%
0.17%
2.13E-01
K18me3R42me1
0.00%
0.00%
2.99E-01
K14me3K18ac
0.01%
0.12%
1.67E-01
K14acR26me1
0.33%
0.18%
2.13E-01
K18me3R49me2
0.00%
0.00%
2.99E-01
K27acR49me2
0.13%
0.03%
1.67E-01
R17me2K36me2
0.69%
0.47%
2.17E-01
K4me1R8me1
0.07%
0.13%
2.99E-01
K14acR42me2
0.05%
0.15%
1.67E-01
K37me1R40me1
0.00%
0.01%
2.20E-01
K9me1K23me1
0.00%
0.01%
3.01E-01
K14me3K37me1
0.00%
0.02%
1.68E-01
K14me2K23me3
0.00%
0.00%
2.21E-01
K36me2K37me2
0.00%
0.01%
3.06E-01
K23me1K27me1
0.61%
0.41%
1.68E-01
K27me1K36me3
0.72%
0.50%
2.23E-01
K27acK36me1
0.14%
0.18%
3.07E-01
R17me2K27me2
0.69%
0.42%
1.68E-01
R26me1K27me1
0.68%
0.90%
2.29E-01
K4me1K23me3
0.00%
0.00%
3.08E-01
K9me3K23me1
0.00%
0.01%
1.68E-01
K9acR17me2
0.43%
0.24%
2.33E-01
R8me1K23me3
0.00%
0.00%
3.08E-01
K9acK18me3
0.00%
0.01%
1.69E-01
K18me1K23ac
2.85%
1.98%
2.34E-01
R8me2K23me3
0.00%
0.00%
3.08E-01
K18me1K27me1
0.10%
0.21%
1.69E-01
K18me1K23me1
0.44%
0.29%
2.36E-01
K9me3K23me3
0.00%
0.00%
3.08E-01
K14me1R17me2
0.00%
0.02%
1.69E-01
K18acR26me1
0.12%
0.24%
2.39E-01
R17me1K23me3
0.00%
0.00%
3.08E-01
K14acK36ac
0.01%
0.00%
1.69E-01
K4me1K36me1
0.60%
0.40%
2.42E-01
R17me2K23me3
0.00%
0.00%
3.08E-01
R8me1K9me2
0.00%
0.07%
1.70E-01
K23acK36ac
0.20%
0.12%
2.43E-01
K18me1K23me3
0.00%
0.00%
3.08E-01
K9acR26me2
0.01%
0.00%
1.70E-01
K14me2K18ac
0.04%
0.11%
2.44E-01
K23me3R26me2
0.00%
0.00%
3.08E-01
K4me1K37me1
0.68%
0.42%
1.70E-01
K14acK18ac
7.41%
5.94%
2.45E-01
K23me3K36me3
0.00%
0.00%
3.08E-01
R8me2R26me1
0.04%
0.00%
1.70E-01
K27me3K36ac
0.02%
0.01%
2.46E-01
K23me3R40me2
0.00%
0.00%
3.08E-01
R8me2K23me2
0.16%
0.00%
1.70E-01
K23me3R42me2
0.00%
0.00%
2.46E-01
K23me3R49me2
0.00%
0.00%
3.08E-01
K9acK37me1
0.12%
0.00%
1.70E-01
K14acR17me2
0.17%
0.07%
2.47E-01
K23me2R26me2
0.04%
0.02%
3.09E-01
K23me1K27me3
0.00%
0.02%
1.71E-01
R17me1K37me1
0.08%
0.16%
2.48E-01
K36acK37me2
0.00%
0.00%
3.11E-01
K23me2K27me1
0.01%
0.00%
1.71E-01
R17me1K18ac
0.16%
0.07%
2.50E-01
K23me3K27me1
0.21%
0.14%
3.13E-01
R8me1K18me1
0.00%
0.02%
1.71E-01
K18me2R42me2
0.00%
0.00%
2.51E-01
K23me3R40me1
0.00%
0.00%
3.15E-01
R8me1K27ac
0.00%
0.02%
1.71E-01
K14me1R17me1
0.12%
0.19%
2.54E-01
K9me1K14ac
3.94%
3.32%
3.18E-01
K4me1K36ac
0.02%
0.00%
1.71E-01
R8me1K23ac
0.22%
0.32%
2.54E-01
K36acR42me2
0.00%
0.00%
3.23E-01
R8me1K36ac
0.02%
0.00%
1.71E-01
K14me2K23ac
0.04%
0.10%
2.59E-01
R26me1K36me3
0.09%
0.14%
3.24E-01
K37me1R49me2
0.01%
0.00%
1.71E-01
K27me1R42me2
0.01%
0.02%
2.78E-01
K9me1R42me1
0.04%
0.06%
3.25E-01
K18acK36ac
0.03%
0.00%
1.71E-01
R8me1K9me1
4.53%
5.10%
2.79E-01
K4me1K37me2
0.00%
0.00%
3.25E-01
K9me3K14me1
0.01%
0.00%
1.72E-01
K4me1R17me1
0.13%
0.07%
2.80E-01
R8me1K37me2
0.00%
0.00%
3.25E-01
R8me2K36me1
0.00%
0.04%
1.72E-01
K9acK14me3
0.05%
0.12%
2.82E-01
R8me2K37me2
0.00%
0.00%
3.25E-01
K18me2K23me3
0.00%
0.00%
1.72E-01
K23me3K37me2
0.00%
0.01%
2.82E-01
K9acK37me2
0.00%
0.00%
3.25E-01
K9me1K37me1
0.32%
0.19%
1.73E-01
R26me1K36ac
0.05%
0.02%
2.88E-01
K9me1K37me2
0.00%
0.00%
3.25E-01
K18me1R26me2
0.00%
0.02%
1.73E-01
K27acR42me2
0.02%
0.01%
2.88E-01
K9me2K37me2
0.00%
0.00%
3.25E-01
R17me1K36ac
0.00%
0.03%
1.73E-01
K14me3K36ac
0.00%
0.00%
2.88E-01
K14acK37me2
0.00%
0.00%
3.25E-01
K18me1K36ac
0.00%
0.01%
1.74E-01
K4me1K37me3
0.00%
0.00%
2.90E-01
K14me1K37me2
0.00%
0.00%
3.25E-01
K18me1R26me1
0.02%
0.00%
1.75E-01
R8me1K37me3
0.00%
0.00%
2.90E-01
R17me1K37me2
0.00%
0.00%
3.25E-01
K18me3K36ac
0.00%
0.01%
1.75E-01
R8me2K37me3
0.00%
0.00%
2.90E-01
R17me2K37me2
0.00%
0.00%
3.25E-01
K23me1R49me2
0.00%
0.01%
1.77E-01
K9acK37me3
0.00%
0.00%
2.90E-01
K18acK37me2
0.00%
0.00%
3.25E-01
K9me1K27me1
0.10%
0.19%
1.79E-01
K9me1K37me3
0.00%
0.00%
2.90E-01
K18me1K37me2
0.00%
0.00%
3.25E-01
K9me3R49me2
0.00%
0.01%
1.79E-01
K9me3K37me3
0.00%
0.00%
2.90E-01
K18me2K37me2
0.00%
0.00%
3.25E-01
K18me3K37me3
0.00%
0.00%
1.81E-01
K14me3K18me2
0.00%
0.00%
2.90E-01
K23me1K37me2
0.00%
0.00%
3.25E-01
K37me3R40me1
0.00%
0.00%
1.81E-01
K14me1K37me3
0.00%
0.00%
2.90E-01
K23me2K37me2
0.00%
0.00%
3.25E-01
K37me3R42me1
0.00%
0.00%
1.81E-01
K14me3K37me3
0.00%
0.00%
2.90E-01
R26me1K37me2
0.00%
0.00%
3.25E-01
K9me3K18me3
0.01%
0.00%
1.84E-01
R17me2K37me3
0.00%
0.00%
2.90E-01
R26me2K37me2
0.00%
0.00%
3.25E-01
K14me1K18me3
0.01%
0.00%
1.84E-01
R17me1K37me3
0.00%
0.00%
2.90E-01
K27acK37me2
0.00%
0.00%
3.25E-01
K18me3K27me2
0.01%
0.00%
1.84E-01
K18me2K37me3
0.00%
0.00%
2.90E-01
K27me1K37me2
0.00%
0.00%
3.25E-01
R26me1R40me2
0.00%
0.06%
1.84E-01
K18me1K37me3
0.00%
0.00%
2.90E-01
K27me2K37me2
0.00%
0.00%
3.25E-01
K9me1K36ac
0.09%
0.04%
1.85E-01
K23me2K37me3
0.00%
0.00%
2.90E-01
K36me1K37me2
0.00%
0.00%
3.25E-01
R17me1K27me1
0.12%
0.03%
1.89E-01
K23me1K37me3
0.00%
0.00%
2.90E-01
K36me3K37me2
0.00%
0.00%
3.25E-01
K9me1K23me3
0.02%
0.00%
1.90E-01
R26me1K37me3
0.00%
0.00%
2.90E-01
K37me2R40me1
0.00%
0.00%
3.25E-01
K14me3K18me3
0.00%
0.01%
1.90E-01
R26me2K37me3
0.00%
0.00%
2.90E-01
K37me2R40me2
0.00%
0.00%
3.25E-01
K4me1K14me2
0.00%
0.00%
1.90E-01
K27me3K37me3
0.00%
0.00%
2.90E-01
K37me2R42me1
0.00%
0.00%
3.25E-01
R8me2K14me2
0.00%
0.00%
1.90E-01
K27acK37me3
0.00%
0.00%
2.90E-01
K37me2R42me2
0.00%
0.00%
3.25E-01
R8me1K14me2
0.00%
0.00%
1.90E-01
K36me2K37me3
0.00%
0.00%
2.90E-01
K37me2R49me2
0.00%
0.00%
3.25E-01
28
Binary co-existence
Table S5 - continues
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
K14me3K23me3
Binary co-existence
0.00%
0.00%
3.29E-01
R8me2R17me2
Binary co-existence
0.00%
0.00%
4.34E-01
K9acK18ac
0.24%
0.22%
4.53E-01
K9me1R40me1
0.04%
0.06%
3.30E-01
R8me2K18me1
0.00%
0.00%
4.34E-01
R8me1K14ac
0.09%
0.08%
4.54E-01
K18acR42me2
0.06%
0.10%
3.31E-01
R8me2K23me1
0.00%
0.00%
4.34E-01
K27me3K36me3
0.11%
0.12%
4.54E-01
K9acK23me1
0.12%
0.17%
3.34E-01
R8me1R26me2
0.00%
0.00%
4.34E-01
K18acK27ac
0.07%
0.06%
4.59E-01
K14acK27ac
0.34%
0.23%
3.35E-01
R8me2R26me2
0.00%
0.00%
4.34E-01
K4me1R40me1
0.00%
0.00%
4.62E-01
K9me3R26me2
0.06%
0.04%
3.45E-01
R8me2K27ac
0.00%
0.00%
4.34E-01
R8me1R40me1
0.00%
0.00%
4.62E-01
K18acK27me3
0.44%
0.67%
3.47E-01
R8me2K27me1
0.00%
0.00%
4.34E-01
R8me2R40me1
0.00%
0.00%
4.62E-01
K9me3K14me3
0.09%
0.13%
3.48E-01
R8me2K27me3
0.00%
0.00%
4.34E-01
K9acR40me1
0.00%
0.00%
4.62E-01
K9acK27ac
0.03%
0.05%
3.52E-01
R8me2K36me3
0.00%
0.00%
4.34E-01
K9me3R40me1
0.00%
0.00%
4.62E-01
K36acK37me3
0.00%
0.00%
3.55E-01
R8me2K37me1
0.00%
0.00%
4.34E-01
K14acR40me1
0.00%
0.00%
4.62E-01
K14acK18me1
1.43%
1.20%
3.59E-01
R8me1R40me2
0.00%
0.00%
4.34E-01
R17me1R40me1
0.00%
0.00%
4.62E-01
K9me2K23me3
0.45%
0.36%
3.60E-01
R8me2R40me2
0.00%
0.00%
4.34E-01
R17me2R40me1
0.00%
0.00%
4.62E-01
R26me1K36me1
0.69%
0.78%
3.64E-01
R8me1R49me2
0.00%
0.00%
4.34E-01
K18acR40me1
0.00%
0.00%
4.62E-01
R8me1K36me2
4.86%
5.22%
3.64E-01
R8me2R49me2
0.00%
0.00%
4.34E-01
K18me1R40me1
0.00%
0.00%
4.62E-01
K4me1R42me1
0.00%
0.00%
3.65E-01
K9me3R17me1
0.00%
0.00%
4.34E-01
K23me1R40me1
0.00%
0.00%
4.62E-01
R8me1R42me1
0.00%
0.00%
3.65E-01
K9me2R17me2
0.00%
0.00%
4.34E-01
K23me2R40me1
0.00%
0.00%
4.62E-01
R8me2R42me1
0.00%
0.00%
3.65E-01
K9me3R17me2
0.00%
0.00%
4.34E-01
R26me2R40me1
0.00%
0.00%
4.62E-01
K9acR42me1
0.00%
0.00%
3.65E-01
K9me3K18me1
0.00%
0.00%
4.34E-01
K27acR40me1
0.00%
0.00%
4.62E-01
K9me3R42me1
0.00%
0.00%
3.65E-01
K9me2K23me2
0.00%
0.00%
4.34E-01
K36me2R40me1
0.00%
0.00%
4.62E-01
R17me1R42me1
0.00%
0.00%
3.65E-01
K9me2K27me2
0.00%
0.00%
4.34E-01
K36me3R40me1
0.00%
0.00%
4.62E-01
R17me2R42me1
0.00%
0.00%
3.65E-01
K9me3K27me3
0.00%
0.00%
4.34E-01
R40me1R49me2
0.00%
0.00%
4.62E-01
K18acR42me1
0.00%
0.00%
3.65E-01
K9me2K36me2
0.00%
0.00%
4.34E-01
K23acR26me2
0.13%
0.12%
4.66E-01
K18me1R42me1
0.00%
0.00%
3.65E-01
K9me3K36me3
0.00%
0.00%
4.34E-01
K14acK27me3
0.91%
0.87%
4.68E-01
K23me1R42me1
0.00%
0.00%
3.65E-01
K9acR40me2
0.00%
0.00%
4.34E-01
K4me1K27ac
0.01%
0.01%
4.69E-01
K23me2R42me1
0.00%
0.00%
3.65E-01
K9me3R40me2
0.00%
0.00%
4.34E-01
K9me2K27ac
0.51%
0.53%
4.70E-01
R26me2R42me1
0.00%
0.00%
3.65E-01
K9me1R49me2
0.00%
0.00%
4.34E-01
K18me3K23me3
0.00%
0.00%
4.70E-01
K36me2R42me1
0.00%
0.00%
3.65E-01
K14me1R26me2
0.00%
0.00%
4.34E-01
K23me2K36me1
0.01%
0.01%
4.74E-01
K36me3R42me1
0.00%
0.00%
3.65E-01
K14me1K27me1
0.00%
0.00%
4.34E-01
K14acK37me3
0.08%
0.09%
4.75E-01
R40me2R42me1
0.00%
0.00%
3.65E-01
K14me1K36me1
0.00%
0.00%
4.34E-01
K36acR40me1
0.00%
0.00%
4.79E-01
R42me1R49me2
0.00%
0.00%
3.65E-01
K14me1K37me1
0.00%
0.00%
4.34E-01
K36acR42me1
0.00%
0.00%
4.79E-01
R8me2K23ac
0.17%
0.11%
3.66E-01
K14me1R40me2
0.00%
0.00%
4.34E-01
K9acK14ac
0.07%
0.07%
4.79E-01
K4me1R26me1
0.12%
0.09%
3.68E-01
K14me1R49me2
0.00%
0.00%
4.34E-01
K9me2R26me2
0.10%
0.10%
4.79E-01
K18me1K27ac
0.37%
0.29%
3.75E-01
R17me2K23me1
0.00%
0.00%
4.34E-01
K4me1R42me2
0.00%
0.00%
4.85E-01
K18me3R42me2
0.00%
0.00%
3.77E-01
R17me2K23me2
0.00%
0.00%
4.34E-01
R8me1R42me2
0.00%
0.00%
4.85E-01
K4me1K18me2
0.00%
0.00%
3.80E-01
R17me2R26me1
0.00%
0.00%
4.34E-01
R8me2R42me2
0.00%
0.00%
4.85E-01
R8me1K18me2
0.00%
0.00%
3.80E-01
R17me2R26me2
0.00%
0.00%
4.34E-01
K9me3R42me2
0.00%
0.00%
4.85E-01
R8me2K18me2
0.00%
0.00%
3.80E-01
R17me2K27me1
0.00%
0.00%
4.34E-01
K14me1R42me2
0.00%
0.00%
4.85E-01
K9me2K18me2
0.00%
0.00%
3.80E-01
R17me2K27me3
0.00%
0.00%
4.34E-01
R17me1R42me2
0.00%
0.00%
4.85E-01
K14me1K18me2
0.00%
0.00%
3.80E-01
R17me2K36me1
0.00%
0.00%
4.34E-01
R17me2R42me2
0.00%
0.00%
4.85E-01
R17me1K18me2
0.00%
0.00%
3.80E-01
R17me2K36me3
0.00%
0.00%
4.34E-01
K18me1R42me2
0.00%
0.00%
4.85E-01
R17me2K18me2
0.00%
0.00%
3.80E-01
R17me2K37me1
0.00%
0.00%
4.34E-01
K23me1R42me2
0.00%
0.00%
4.85E-01
K18me2R26me1
0.00%
0.00%
3.80E-01
R17me1R40me2
0.00%
0.00%
4.34E-01
K23me2R42me2
0.00%
0.00%
4.85E-01
K18me2R26me2
0.00%
0.00%
3.80E-01
R17me2R40me2
0.00%
0.00%
4.34E-01
R26me2R42me2
0.00%
0.00%
4.85E-01
K18me2R40me2
0.00%
0.00%
3.80E-01
R17me1R49me2
0.00%
0.00%
4.34E-01
K27me3R42me2
0.00%
0.00%
4.85E-01
K18me2R49me2
0.00%
0.00%
3.80E-01
R17me2R49me2
0.00%
0.00%
4.34E-01
K36me1R42me2
0.00%
0.00%
4.85E-01
K9me2K37me3
0.16%
0.20%
3.84E-01
K18me1K27me3
0.00%
0.00%
4.34E-01
K36me2R42me2
0.00%
0.00%
4.85E-01
K14acK23ac
2.65%
2.97%
3.86E-01
K18me1K36me1
0.00%
0.00%
4.34E-01
K36me3R42me2
0.00%
0.00%
4.85E-01
K23acK27ac
0.33%
0.43%
3.88E-01
K18me1K37me1
0.00%
0.00%
4.34E-01
K37me1R42me2
0.00%
0.00%
4.85E-01
K14me1K23me1
0.09%
0.06%
3.89E-01
K18me1R40me2
0.00%
0.00%
4.34E-01
R40me2R42me2
0.00%
0.00%
4.85E-01
R8me1K23me1
0.02%
0.02%
3.90E-01
K18me1R49me2
0.00%
0.00%
4.34E-01
R26me2K27me2
0.04%
0.04%
4.91E-01
K18me3R40me1
0.00%
0.00%
3.91E-01
K23me1R26me1
0.00%
0.00%
4.34E-01
K23me3K37me3
0.00%
0.00%
4.96E-01
K14me3K36me2
0.17%
0.14%
3.96E-01
K23me1R26me2
0.00%
0.00%
4.34E-01
K4acR8me2
0.00%
0.00%
NA
K23me1K27ac
0.23%
0.20%
3.97E-01
K23me2K36me3
0.00%
0.00%
4.34E-01
K4acR8me1
0.05%
0.00%
NA
K18me3K37me2
0.00%
0.00%
4.00E-01
K23me2K37me1
0.00%
0.00%
4.34E-01
K4me2R8me2
0.00%
0.00%
NA
K23me3R42me1
0.00%
0.00%
4.02E-01
K23me1R40me2
0.00%
0.00%
4.34E-01
K4me3R8me1
0.00%
0.00%
NA
K23me2K36ac
0.01%
0.02%
4.03E-01
K23me2R40me2
0.00%
0.00%
4.34E-01
K4me3R8me2
0.00%
0.00%
NA
R40me1R42me1
0.22%
0.28%
4.03E-01
K23me2R49me2
0.00%
0.00%
4.34E-01
K4me2R8me1
0.00%
0.00%
NA
K27acK36me2
0.23%
0.26%
4.04E-01
R26me1K27ac
0.00%
0.00%
4.34E-01
K4acK9ac
0.00%
0.00%
NA
R8me2K36ac
0.00%
0.00%
4.10E-01
R26me2K27me3
0.00%
0.00%
4.34E-01
K4acK9me2
0.00%
0.00%
NA
K9acK36ac
0.00%
0.00%
4.10E-01
R26me1R49me2
0.00%
0.00%
4.34E-01
K4acK9me3
0.00%
0.00%
NA
R17me2K36ac
0.00%
0.00%
4.10E-01
R26me2R49me2
0.00%
0.00%
4.34E-01
K4acK9me1
0.08%
0.00%
NA
K27acK36ac
0.00%
0.00%
4.10E-01
K36me1R40me2
0.00%
0.00%
4.34E-01
K4me3K9me1
0.00%
0.00%
NA
K36acK37me1
0.00%
0.00%
4.10E-01
K36me2R40me2
0.00%
0.00%
4.34E-01
K4me2K9me2
0.00%
0.00%
NA
K36acR40me2
0.00%
0.00%
4.10E-01
K36me3R40me2
0.00%
0.00%
4.34E-01
K4me3K9me2
0.00%
0.00%
NA
K36acR49me2
0.00%
0.00%
4.10E-01
K36me1R49me2
0.00%
0.00%
4.34E-01
K4me3K9me3
0.00%
0.46%
NA
K14acR17me1
0.20%
0.23%
4.18E-01
K36me2R49me2
0.00%
0.00%
4.34E-01
K4me2K9me3
0.00%
0.00%
NA
K37me3R42me2
0.00%
0.00%
4.19E-01
K36me3R49me2
0.00%
0.00%
4.34E-01
K4me2K9ac
0.00%
0.00%
NA
K14acK23me1
0.24%
0.21%
4.22E-01
K37me1R40me2
0.00%
0.00%
4.34E-01
K4me2K9me1
0.00%
0.00%
NA
K23acR49me2
0.25%
0.29%
4.22E-01
R40me2R49me2
0.00%
0.00%
4.34E-01
K4me3K9ac
0.00%
0.00%
NA
R40me1R42me2
0.00%
0.00%
4.27E-01
R8me2K9ac
0.00%
0.00%
4.34E-01
K4acK14me1
0.00%
0.00%
NA
K4me1R17me2
0.00%
0.00%
4.34E-01
R8me2K9me1
0.00%
0.00%
4.34E-01
K4acK14ac
0.00%
0.00%
NA
K4me1K18me1
0.00%
0.00%
4.34E-01
R8me2K9me2
0.00%
0.00%
4.34E-01
K4acK14me3
0.00%
0.00%
NA
K4me1K23me1
0.00%
0.00%
4.34E-01
R8me1K9me3
0.00%
0.00%
4.34E-01
K4me3K14me1
0.00%
0.00%
NA
K4me1K23me2
0.00%
0.00%
4.34E-01
R8me2K9me3
0.00%
0.00%
4.34E-01
K4me2K14me1
0.00%
0.15%
NA
K4me1R26me2
0.00%
0.00%
4.34E-01
R8me1K27me2
4.86%
5.01%
4.42E-01
K4me3K14me3
0.00%
0.00%
NA
K4me1R40me2
0.00%
0.00%
4.34E-01
K18me2R40me1
0.00%
0.00%
4.45E-01
K4me2K14ac
0.00%
0.13%
NA
K4me1R49me2
0.00%
0.00%
4.34E-01
K18me2R42me1
0.00%
0.00%
4.45E-01
K4me2K14me3
0.00%
0.00%
NA
R8me2K14me1
0.00%
0.00%
4.34E-01
R26me2K36ac
0.02%
0.02%
4.47E-01
K4me3K14ac
0.15%
0.00%
NA
R8me2R17me1
0.00%
0.00%
4.34E-01
R8me1K37me1
0.05%
0.06%
4.47E-01
K4me3K14me2
0.00%
0.00%
NA
R8me1R17me2
0.00%
0.00%
4.34E-01
K9acK36me1
0.12%
0.14%
4.53E-01
K4acK14me2
0.00%
0.00%
NA
29
Binary co-existence
Table S5 - continues
Binary co-existence
Wild type
K4me2K14me2
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
0.00%
NA
K4me3K27me3
Binary co-existence
0.00%
0.00%
NA
K4me2R49me1
Binary co-existence
Wild type
Suz12-/-
t-test
0.00%
NA
K4acR17me1
0.00%
0.00%
NA
K4me2K27me3
0.15%
0.00%
NA
R8me1R49me1
0.00%
0.00%
NA
K4acR17me2
0.00%
0.00%
NA
K4me2K27me1
0.00%
0.22%
NA
R8me2R49me1
0.00%
0.00%
NA
K4me2R17me1
0.00%
0.00%
NA
K4me3K27ac
0.00%
0.00%
NA
K9me3R49me1
0.00%
0.00%
NA
K4me3R17me1
0.00%
0.00%
NA
K4me2K27ac
0.00%
0.00%
NA
K9acR49me1
0.00%
0.00%
NA
K4me2R17me2
0.00%
0.00%
NA
K4acK36me3
0.08%
0.00%
NA
K9me2R49me1
0.03%
0.10%
NA
K4me3R17me2
0.00%
0.00%
NA
K4acK36ac
0.00%
NA
K9me1R49me1
0.05%
0.05%
NA
K4acK18ac
0.00%
0.00%
NA
K4acK36me2
0.05%
0.00%
NA
K14me2K18me2
0.00%
0.00%
NA
K4acK18me2
0.00%
0.00%
NA
K4acK36me1
0.00%
0.00%
NA
K14me2K36ac
0.00%
0.00%
NA
K4acK18me1
0.00%
0.00%
NA
K4me3K36me3
0.00%
0.00%
NA
K14me3K37me2
0.00%
0.00%
NA
K4me3K18me2
0.00%
0.00%
NA
K4me2K36me3
0.00%
0.24%
NA
K14me2K37me2
0.00%
0.00%
NA
K4me2K18me2
0.00%
0.00%
NA
K4me2K36ac
0.00%
0.00%
NA
K14me2K37me3
0.00%
0.00%
NA
K4me3K18me1
0.00%
0.00%
NA
K4me3K36me2
0.33%
0.46%
NA
K14me2R40me1
0.00%
0.00%
NA
K4me2K18me1
0.00%
0.00%
NA
K4me3K36ac
0.00%
0.00%
NA
K14acR49me1
0.00%
0.03%
NA
K4me2K18me3
0.00%
0.00%
NA
K4me2K36me1
0.15%
0.13%
NA
K14me1R49me1
0.00%
0.00%
NA
K4me3K18ac
0.00%
0.00%
NA
K4me2K36me2
0.00%
0.00%
NA
K14me2R49me1
0.00%
0.00%
NA
K4me3K18me3
0.00%
0.00%
NA
K4me3K36me1
0.04%
0.00%
NA
K14me3R49me1
0.00%
0.00%
NA
K4me2K18ac
0.00%
0.13%
NA
K4acK37me3
0.00%
0.00%
NA
R17me1R49me1
0.00%
0.00%
NA
K4acK18me3
0.00%
0.00%
NA
K4acK37me1
0.00%
0.00%
NA
R17me2R49me1
0.00%
0.00%
NA
K4acK23me1
0.00%
0.00%
NA
K4me3K37me1
0.00%
0.00%
NA
K18acR49me1
0.00%
0.00%
NA
K4acK23ac
0.00%
0.00%
NA
K4me2K37me1
0.15%
0.13%
NA
K18me1R49me1
0.00%
0.00%
NA
K4acK23me3
0.00%
0.00%
NA
K4me3K37me3
0.00%
0.00%
NA
K18me3R49me1
0.00%
0.00%
NA
K4acK23me2
0.00%
0.00%
NA
K4me2K37me3
0.00%
0.00%
NA
K18me2R49me1
0.00%
NA
K4me3K23me3
0.00%
NA
K4me3K37me2
0.00%
NA
K23acR49me1
0.05%
0.03%
NA
K4me2K23me3
0.00%
0.00%
NA
K4me2K37me2
0.00%
NA
K23me1R49me1
0.00%
0.00%
NA
K4me3K23me1
0.00%
0.00%
NA
K4acK37me2
0.00%
NA
K23me3R49me1
0.00%
0.00%
NA
K4me3K23me2
0.00%
0.00%
NA
K4acR40me2
0.00%
0.00%
NA
K23me2R49me1
0.00%
0.00%
NA
K4me2K23me2
0.00%
0.00%
NA
K4me3R40me1
0.00%
0.00%
NA
R26me1R49me1
0.00%
0.00%
NA
K4me2K23ac
0.15%
0.24%
NA
K4me2R40me1
0.00%
0.00%
NA
R26me2R49me1
0.00%
0.00%
NA
K4me3K23ac
0.10%
0.00%
NA
K4acR40me1
0.00%
0.00%
NA
K27me2R49me1
0.00%
0.00%
NA
K4me2K23me1
0.00%
0.00%
NA
K4me3R40me2
0.00%
0.00%
NA
K27me3R49me1
0.03%
0.00%
NA
K4acR26me2
0.00%
0.00%
NA
K4me2R40me2
0.00%
0.00%
NA
K27acR49me1
0.05%
0.00%
NA
K4acR26me1
0.00%
0.00%
NA
K4acR42me2
0.00%
0.00%
NA
K27me1R49me1
0.00%
0.07%
NA
K4me3R26me1
0.00%
0.00%
NA
K4acR42me1
0.00%
0.00%
NA
K36me2R49me1
0.00%
0.00%
NA
K4me2R26me1
0.00%
0.00%
NA
K4me3R42me1
0.00%
0.00%
NA
K36me3R49me1
0.00%
0.00%
NA
K4me3R26me2
0.00%
0.00%
NA
K4me2R42me1
0.00%
0.00%
NA
K36me1R49me1
0.00%
0.07%
NA
K4me2R26me2
0.00%
0.00%
NA
K4me3R42me2
0.00%
0.00%
NA
K36acR49me1
0.00%
NA
K4acK27me1
0.00%
0.00%
NA
K4me2R42me2
0.00%
NA
K37me1R49me1
0.00%
NA
K4acK27me3
0.03%
0.00%
NA
K4acR49me2
0.00%
0.00%
NA
K37me2R49me1
0.00%
NA
K4acK27ac
0.00%
0.00%
NA
K4me3R49me2
0.00%
0.00%
NA
K37me3R49me1
0.00%
NA
K4acK27me2
0.05%
0.00%
NA
K4me2R49me2
0.00%
0.00%
NA
R40me2R49me1
0.00%
NA
K4me3K27me1
0.00%
0.00%
NA
K4me3R49me1
0.00%
0.00%
NA
R40me1R49me1
0.00%
NA
K4me3K27me2
0.37%
0.00%
NA
K4acR49me1
0.00%
0.00%
NA
R42me1R49me1
0.05%
0.05%
NA
K4me2K27me2
0.00%
0.00%
NA
K4me1R49me1
0.00%
0.00%
NA
R42me2R49me1
0.00%
0.00%
NA
30
0.00%
0.00%
Table S6
Binary co-existence
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
Wild type
Suz12-/-
t-test
59.70%
5.64%
4.30E-07
K27me3R49me2
1.10%
0.01%
1.36E-01
R26me1K37me1
0.01%
0.01%
2.92E-01
K9me2K18ac
0.01%
2.63%
1.35E-04
K18me1K23me1
1.08%
0.01%
1.38E-01
K18acK27me2
2.25%
1.24%
3.02E-01
K9me1K27me2
31.66%
2.30%
2.26E-04
K9me1K23me1
0.55%
0.01%
1.40E-01
R17me1K23me1
0.01%
0.01%
3.05E-01
K9me3K27me2
10.23%
2.35%
2.84E-04
K14me1K23me1
0.55%
0.01%
1.40E-01
K14acR26me1
0.01%
0.01%
3.19E-01
K27me2K36me1
9.46%
0.20%
3.96E-04
K23me1K27me1
0.55%
0.01%
1.40E-01
K9acR49me2
0.01%
0.01%
3.26E-01
K14me1K27me2
14.20%
0.76%
7.01E-04
K9acK23me1
0.52%
0.01%
1.40E-01
K9me1R49me2
0.01%
0.01%
3.26E-01
K27me1K36me1
0.99%
10.20%
7.93E-04
K9me3K23me1
0.13%
0.01%
1.41E-01
K9me3R49me2
0.01%
0.01%
3.26E-01
K9me2K27me1
0.12%
11.83%
9.25E-04
K23me1K27me2
0.13%
0.01%
1.41E-01
K14me1R49me2
0.01%
0.01%
3.26E-01
K27me1K37me1
0.01%
2.00%
1.63E-03
K14acK23ac
0.32%
0.01%
1.41E-01
K18acR49me2
0.01%
0.01%
3.26E-01
K23acK27me2
16.49%
1.46%
1.73E-03
K9acR17me2
0.09%
0.01%
1.44E-01
K18me1R49me2
0.01%
0.01%
3.26E-01
K9me2K36me1
0.49%
17.09%
2.61E-03
K9me1K18me1
1.02%
0.37%
1.49E-01
K36me2R49me2
0.01%
0.01%
3.26E-01
K18acK36me1
0.01%
1.77%
3.69E-03
R17me2R26me1
0.01%
0.02%
1.49E-01
K36me3R49me2
0.01%
0.01%
3.26E-01
R26me2K27me2
1.26%
0.02%
5.30E-03
R17me2R26me2
0.01%
0.02%
1.49E-01
K37me1R49me2
0.01%
0.01%
3.26E-01
K9me2K36me3
1.04%
4.51%
8.79E-03
R17me2K27me3
0.01%
0.02%
1.49E-01
K14me3K23ac
0.50%
0.32%
3.38E-01
K9me2K37me1
0.23%
5.83%
1.11E-02
K9me1R17me1
1.34%
0.01%
1.50E-01
K23acK36me1
3.02%
3.66%
3.39E-01
K9me3K36me1
1.38%
0.21%
1.14E-02
R8me1K37me1
0.01%
0.76%
1.57E-01
K14me1K36me2
17.63%
16.50%
3.61E-01
K9me2K23ac
1.24%
5.31%
1.19E-02
K23acK37me1
0.55%
1.05%
1.67E-01
K14me1K23ac
3.89%
4.70%
3.67E-01
K9me3R26me1
1.16%
0.01%
1.24E-02
K9me2K27me3
2.52%
0.85%
1.70E-01
K18me1K23ac
0.65%
0.87%
3.69E-01
K36me1K37me1
1.05%
5.63%
1.50E-02
K9me2K14me1
0.01%
0.41%
1.79E-01
R17me1R49me2
0.01%
0.01%
3.78E-01
K27me1K36me2
1.77%
0.01%
1.87E-02
K9me1K27me1
0.28%
0.01%
1.79E-01
K9me3K23ac
0.47%
0.74%
3.82E-01
K9acK27me1
0.01%
0.47%
1.89E-02
K14me1K27me1
0.28%
0.01%
1.79E-01
R26me1K27me1
0.79%
0.62%
3.87E-01
K9acK36me1
0.01%
0.47%
1.89E-02
K18me1K27me1
0.28%
0.01%
1.79E-01
R17me1K23ac
0.44%
0.25%
3.93E-01
K9me1K36me3
2.19%
0.05%
1.98E-02
K18acK36me3
0.01%
0.46%
1.79E-01
K14me1K18me1
1.37%
1.22%
3.95E-01
K9acK37me1
0.01%
0.46%
2.10E-02
R8me1K9me2
0.01%
1.03%
1.80E-01
K18acK23ac
1.34%
1.02%
3.95E-01
K18acK27me1
0.20%
1.72%
2.11E-02
K14acR26me2
0.01%
0.02%
1.81E-01
K9acK14me3
0.34%
0.25%
3.96E-01
R8me1K27me2
2.15%
0.59%
2.37E-02
K9me3K14me3
0.26%
0.01%
1.83E-01
R17me2K36me2
0.68%
1.03%
4.07E-01
R8me1K36me2
2.15%
0.59%
2.37E-02
K9me3K14me1
0.01%
0.07%
1.83E-01
K18acK36me2
1.75%
2.15%
4.09E-01
K9me3K36me2
8.85%
5.30%
2.39E-02
K9me2K14me3
0.01%
0.32%
1.86E-01
K27me1K36me3
0.64%
0.78%
4.16E-01
K14me1K18ac
0.01%
1.69%
2.54E-02
R8me1K23me1
0.01%
0.01%
1.86E-01
K9me1K14me3
0.01%
0.01%
4.30E-01
K23acK27me3
1.41%
0.30%
3.06E-02
K18acK23me1
0.01%
0.01%
1.86E-01
K14me3K18ac
0.01%
0.01%
4.30E-01
K23acK36me2
12.96%
5.99%
3.61E-02
K23me1K36me3
0.01%
0.01%
1.86E-01
K14me3K18me1
0.01%
0.01%
4.30E-01
K14acK18me1
0.48%
0.01%
3.78E-02
K23me1K36me1
0.01%
0.01%
1.86E-01
K14me3K27me1
0.01%
0.01%
4.30E-01
R26me1K36me2
2.56%
0.01%
3.95E-02
K23me1K37me1
0.01%
0.01%
1.86E-01
K14me3K36me1
0.01%
0.01%
4.30E-01
R26me1K27me2
2.32%
0.01%
4.34E-02
K14acK27me3
0.67%
0.01%
1.87E-01
K14me3K36me3
0.01%
0.01%
4.30E-01
R17me1K36me2
2.09%
0.01%
4.56E-02
K18me1K36me2
2.02%
1.37%
1.90E-01
K14me3K37me1
0.01%
0.01%
4.30E-01
K18me1K27me2
0.89%
0.01%
4.66E-02
R8me1R26me1
0.01%
0.01%
1.92E-01
K14acK27me2
0.65%
0.55%
4.31E-01
K9me1K36me2
34.45%
26.12%
4.72E-02
R8me1K27me3
0.01%
0.01%
1.92E-01
K14acK36me2
0.65%
0.55%
4.31E-01
K9me1K27me3
2.05%
0.17%
5.10E-02
K14me1K36me3
0.55%
0.05%
1.94E-01
K9acR17me1
0.01%
0.01%
4.33E-01
K14me3K27me2
0.83%
0.35%
5.46E-02
K9acK27me2
0.82%
0.19%
2.02E-01
K9me3R17me1
0.01%
0.01%
4.33E-01
K14me3K36me2
0.83%
0.35%
5.46E-02
K14acK36me3
0.01%
0.52%
2.17E-01
K14me1R17me1
0.01%
0.01%
4.33E-01
K9me2R26me1
0.12%
0.52%
5.52E-02
K14acK18ac
0.01%
0.63%
2.17E-01
R17me1K18ac
0.01%
0.01%
4.33E-01
R8me1R26me2
0.01%
0.02%
5.56E-02
K18me1K27me3
0.15%
0.01%
2.19E-01
R17me1K18me1
0.01%
0.01%
4.33E-01
K23me1R26me2
0.01%
0.02%
5.56E-02
K27me3K36me3
0.46%
0.01%
2.21E-01
R17me1K36me3
0.01%
0.01%
4.33E-01
K23me1R26me1
0.01%
0.02%
5.56E-02
K27me3K37me1
0.23%
0.01%
2.21E-01
K9acK18ac
0.01%
0.01%
4.38E-01
K23me1K27me3
0.01%
0.02%
5.56E-02
R26me1K36me3
0.35%
0.01%
2.21E-01
K9me3K18ac
0.01%
0.01%
4.38E-01
K27me3K36me2
3.34%
0.01%
6.09E-02
K9acK14me1
0.36%
0.87%
2.21E-01
K9me2K18me1
0.01%
0.01%
4.38E-01
K23acK27me1
0.76%
2.41%
6.18E-02
K9acK23ac
0.36%
0.87%
2.21E-01
K9me3K18me1
0.01%
0.01%
4.38E-01
K18acK37me1
0.01%
0.51%
6.50E-02
K23acR26me1
0.20%
0.01%
2.24E-01
K9me3K27me1
0.01%
0.01%
4.38E-01
K27me2K36me3
1.16%
0.01%
7.10E-02
R8me1R17me1
0.87%
0.09%
2.33E-01
K9me2K27me2
0.01%
0.01%
4.38E-01
R17me1K27me2
1.93%
0.01%
7.82E-02
K14me3R49me2
0.01%
0.01%
2.42E-01
K9me1K36me1
0.01%
0.01%
4.38E-01
K9me1K23ac
10.79%
4.13%
7.83E-02
K9me2K23me1
0.01%
0.95%
2.47E-01
K9me2K36me2
0.01%
0.01%
4.38E-01
K27me3K36me1
2.96%
0.71%
8.03E-02
K9me3K37me1
0.06%
0.02%
2.49E-01
K9acK36me3
0.01%
0.01%
4.38E-01
K9me2K14ac
0.31%
2.59%
8.22E-02
K9me2R17me2
0.01%
0.31%
2.49E-01
K9me3K36me3
0.01%
0.01%
4.38E-01
K23acR49me2
0.29%
0.01%
8.58E-02
R17me2K18ac
0.01%
0.31%
2.49E-01
K9me1K37me1
0.01%
0.01%
4.38E-01
K27me2R49me2
0.53%
0.01%
8.72E-02
R17me2K23ac
0.01%
0.17%
2.50E-01
K14me1K36me1
0.01%
0.01%
4.38E-01
R8me1K23ac
0.87%
0.01%
9.05E-02
R17me2K36me1
0.01%
0.17%
2.50E-01
K14me1K37me1
0.01%
0.01%
4.38E-01
R17me2K27me2
0.68%
0.01%
9.19E-02
R26me2K36me2
0.57%
0.02%
2.50E-01
K18me1K36me1
0.01%
0.01%
4.38E-01
K27me2K37me1
0.71%
0.01%
9.37E-02
R8me1K9me3
0.01%
0.01%
2.58E-01
K18me1K36me3
0.01%
0.01%
4.38E-01
K9me2R17me1
0.01%
0.16%
9.51E-02
R8me1K9ac
0.01%
0.01%
2.58E-01
K18me1K37me1
0.01%
0.01%
4.38E-01
R17me1K36me1
0.01%
0.16%
9.51E-02
R8me1K18me1
0.01%
0.01%
2.58E-01
K36me2K37me1
0.01%
0.01%
4.38E-01
R17me1K37me1
0.01%
0.16%
9.51E-02
R8me1K36me1
0.01%
0.01%
2.58E-01
K36me3K37me1
0.01%
0.01%
4.38E-01
K23me1K36me2
1.20%
0.01%
9.69E-02
R8me1K36me3
0.01%
0.01%
2.58E-01
K14acR17me1
0.01%
0.01%
4.40E-01
K14acK27me1
0.01%
1.16%
1.01E-01
R8me1K14me1
0.43%
0.21%
2.62E-01
K14me3R17me1
0.01%
0.01%
4.40E-01
R17me1K27me1
0.01%
0.09%
1.01E-01
R8me1K27me1
0.01%
0.05%
2.62E-01
K9acK14ac
0.01%
0.01%
4.41E-01
K9acR26me2
0.01%
0.02%
1.16E-01
K14acK37me1
0.13%
0.66%
2.63E-01
K23acK36me3
1.18%
1.29%
4.46E-01
K9me1R26me2
0.01%
0.02%
1.16E-01
R26me1K36me1
0.79%
0.41%
2.64E-01
K14me3R26me1
0.01%
0.01%
4.46E-01
K9me2R26me2
0.01%
0.02%
1.16E-01
K9me1K18ac
1.03%
1.75%
2.65E-01
K14me3K27me3
0.01%
0.01%
4.46E-01
K9me3R26me2
0.01%
0.02%
1.16E-01
K9me3K14ac
0.36%
0.19%
2.69E-01
K9me1K14me1
15.13%
14.76%
4.47E-01
K14me1R26me2
0.01%
0.02%
1.16E-01
R8me1K9me1
1.23%
0.64%
2.69E-01
K9me1K14ac
0.18%
0.22%
4.48E-01
K18me1R26me2
0.01%
0.02%
1.16E-01
K27me1R49me2
0.01%
0.12%
2.72E-01
K9me2R49me2
1.37%
1.23%
4.56E-01
R26me2K27me1
0.01%
0.02%
1.16E-01
K14acR49me2
0.01%
0.31%
2.74E-01
R26me1R49me2
0.01%
0.01%
4.57E-01
R26me2K27me3
0.01%
0.02%
1.16E-01
K36me1R49me2
0.01%
0.11%
2.80E-01
K9acK18me1
0.79%
0.87%
4.61E-01
R26me2K36me1
0.01%
0.02%
1.16E-01
K9acR26me1
0.01%
0.01%
2.92E-01
R8me1K18ac
0.06%
0.07%
4.80E-01
R26me2K36me3
0.01%
0.02%
1.16E-01
K9acK27me3
0.01%
0.01%
2.92E-01
K9me1R17me2
0.01%
0.01%
4.85E-01
R26me2K37me1
0.01%
0.02%
1.16E-01
K9me3K27me3
0.01%
0.01%
2.92E-01
K9me3R17me2
0.01%
0.01%
4.85E-01
K14acK36me1
0.13%
1.35%
1.23E-01
K14me1R26me1
0.01%
0.01%
2.92E-01
K14me1R17me2
0.01%
0.01%
4.85E-01
K18acR26me2
0.01%
0.18%
1.27E-01
K18acR26me1
0.01%
0.01%
2.92E-01
R17me2K18me1
0.01%
0.01%
4.85E-01
K23acR26me2
0.01%
0.18%
1.27E-01
K18me1R26me1
0.01%
0.01%
2.92E-01
R17me2K27me1
0.01%
0.01%
4.85E-01
K9me1R26me1
1.16%
0.01%
1.30E-01
K18acK27me3
0.01%
0.01%
2.92E-01
R17me2K36me3
0.01%
0.01%
4.85E-01
K14me1K27me3
1.52%
0.14%
1.34E-01
R26me1K27me3
0.01%
0.01%
2.92E-01
R17me2K37me1
0.01%
0.01%
4.85E-01
K27me2K36me2
Binary co-existence
31
Binary co-existence
Table S6 - continues
Binary co-existence
Wild type
Suz12-/-
t-test
Suz12-/-
t-test
1.08%
1.05%
4.87E-01
K4me2K36me2
0.00%
NA
K9me2K18me3
0.02%
K4me3R8me2
0.02%
NA
K4me2K36me1
0.00%
NA
K9me3K18me3
0.02%
K4me1R8me2
0.02%
NA
K4me2K36me3
0.00%
NA
K9me1K18me2
0.00%
0.01%
NA
K4me3R8me1
0.02%
NA
K4me1K37me1
0.12%
NA
K9me2K18me2
0.00%
0.01%
NA
K4me1R8me1
0.01%
NA
K4me3K37me1
0.02%
NA
K9acK18me2
0.00%
0.01%
NA
K4me2R8me1
0.00%
NA
K4me2K37me2
1.69%
NA
K9me3K18me2
0.00%
0.01%
NA
K9acK36me2
Binary co-existence
Wild type
0.02%
Binary co-existence
Wild type
Suz12-/-
t-test
NA
NA
K4me1K9ac
0.02%
0.01%
NA
K4me2K37me1
0.00%
NA
K9acK23me3
0.02%
0.01%
NA
K4me1K9me1
0.02%
0.73%
NA
K4me1K37me2
0.01%
NA
K9me1K23me3
0.02%
0.30%
NA
K4me1K9me2
0.02%
0.01%
NA
K4me1K37me3
0.01%
NA
K9me2K23me3
0.02%
0.01%
NA
K4me1K9me3
0.02%
0.39%
NA
K4me2K37me3
0.00%
NA
K9me3K23me3
0.02%
0.01%
NA
K4me3K9me3
2.95%
NA
K4me3R40me2
0.02%
NA
K9me1K23me2
0.01%
NA
K4me3K9ac
0.02%
NA
K4me1R40me2
0.01%
NA
K9me3K23me2
0.01%
NA
K4me3K9me1
0.02%
NA
K4me2R40me2
0.00%
NA
K9me2K23me2
0.01%
NA
K4me3K9me2
0.02%
NA
K4me1R42me2
0.01%
NA
K9acK23me2
0.01%
NA
K4me2K9me2
0.00%
NA
K4me3R42me2
0.02%
NA
K9acK27ac
0.01%
0.02%
NA
K4me2K9me1
0.00%
NA
K4me2R42me1
0.00%
NA
K9me1K27ac
0.01%
1.10%
NA
K4me2K9ac
0.00%
NA
K4me2R42me2
0.00%
NA
K9me2K27ac
0.89%
0.02%
NA
K4me2K9me3
0.00%
NA
K4me1R42me1
0.00%
NA
K9me3K27ac
0.01%
0.02%
NA
0.01%
NA
K9me3K36ac
0.60%
NA
K4me1K14ac
0.02%
0.01%
NA
K4me1R49me2
0.02%
K4me1K14me1
0.02%
0.01%
NA
K4me3R49me2
0.02%
NA
K9acK36ac
0.02%
NA
K4me1K14me3
0.02%
0.01%
NA
K4me2R49me2
0.00%
NA
K9me1K36ac
0.02%
NA
K4me3K14me2
0.02%
NA
K4me2R49me1
0.00%
NA
K9me2K36ac
0.02%
NA
K4me3K14me1
0.02%
NA
K4me1R49me1
0.00%
NA
K9acK37me2
0.00%
0.01%
NA
K4me3K14ac
0.02%
NA
R8me2K9ac
0.01%
NA
K9me3K37me2
0.00%
0.01%
NA
K4me1K14me2
0.01%
NA
R8me2K9me1
0.01%
NA
K9me1K37me2
0.00%
0.01%
NA
K4me3K14me3
0.02%
NA
R8me2K9me2
0.01%
NA
K9me2K37me2
0.00%
0.01%
NA
K4me2K14me1
0.00%
NA
R8me2K9me3
0.01%
NA
K9acK37me3
0.01%
NA
K4me2K14me3
0.00%
NA
R8me1K14ac
0.00%
0.01%
NA
K9me3K37me3
0.01%
NA
K4me2K14ac
0.00%
NA
R8me1K14me2
0.00%
0.01%
NA
K9me2K37me3
2.11%
NA
K4me2K14me2
0.00%
NA
R8me1K14me3
0.00%
0.01%
NA
K9me1K37me3
0.01%
NA
K4me1R17me1
0.02%
0.01%
NA
R8me2K14me1
0.01%
NA
K9me1R40me2
0.01%
NA
K4me1R17me2
0.02%
0.01%
NA
R8me2K14me2
0.01%
NA
K9me2R40me2
0.62%
NA
K4me3R17me2
0.02%
NA
R8me2K14ac
0.50%
NA
K9me3R40me2
0.01%
NA
K4me3R17me1
0.02%
NA
R8me2K14me3
0.01%
NA
K9acR40me2
0.01%
NA
K4me2R17me2
0.00%
NA
R8me1R17me2
0.01%
NA
K9me1R40me1
0.00%
NA
K4me2R17me1
0.00%
NA
R8me2R17me2
0.02%
NA
K9me3R40me1
0.00%
NA
0.02%
NA
K9acR40me1
0.00%
NA
0.01%
NA
K9me2R40me1
0.00%
NA
0.01%
K4me1K18ac
0.02%
0.01%
NA
R8me2R17me1
K4me1K18me1
0.02%
0.01%
NA
R8me1K18me2
K4me1K18me3
0.02%
NA
R8me2K18me2
0.01%
NA
K9me1R42me1
0.01%
0.00%
NA
0.00%
K4me3K18me1
0.02%
NA
R8me2K18me1
0.01%
NA
K9me3R42me1
0.01%
0.00%
NA
K4me1K18me2
0.01%
NA
R8me2K18ac
1.04%
NA
K9me2R42me1
0.01%
0.00%
NA
K4me3K18ac
0.02%
NA
R8me2K23ac
0.01%
NA
K9acR42me1
0.01%
0.00%
NA
K4me3K18me2
0.02%
NA
R8me2K23me3
0.02%
NA
K9me1R42me2
0.01%
0.01%
NA
K4me2K18me1
0.00%
NA
R8me1K23me3
0.01%
NA
K9me2R42me2
0.01%
0.14%
NA
K4me2K18me2
0.00%
NA
R8me2K23me1
0.02%
NA
K9me3R42me2
0.01%
0.01%
NA
K4me2K18ac
0.65%
NA
R8me2K23me2
0.02%
NA
K9acR42me2
0.01%
0.01%
NA
K4me1K23ac
0.02%
0.73%
NA
R8me1K23me2
0.01%
NA
K9acR49me1
0.01%
0.00%
NA
K4me1K23me3
0.02%
0.01%
NA
R8me2R26me1
0.01%
NA
K9me3R49me1
0.01%
0.73%
NA
K4me3K23ac
2.95%
NA
R8me2R26me2
0.02%
NA
K9me2R49me1
0.38%
0.00%
NA
K4me3K23me3
0.02%
NA
R8me1K27ac
0.02%
NA
K9me1R49me1
0.01%
0.00%
NA
K4me3K23me1
0.02%
NA
R8me2K27me1
0.01%
NA
K14acR17me2
0.02%
0.01%
NA
K4me1K23me1
0.01%
NA
R8me2K27me3
0.01%
NA
K14me3R17me2
0.02%
0.01%
NA
K4me3K23me2
0.02%
NA
R8me2K27ac
0.02%
NA
K14me2R17me1
0.00%
0.01%
NA
K4me1K23me2
0.01%
NA
R8me2K27me2
0.93%
NA
K14me2R17me2
0.01%
NA
K4me2K23me1
0.00%
NA
R8me2K36me1
0.01%
NA
K14acK18me3
0.02%
NA
K4me2K23ac
1.04%
NA
R8me2K36me3
0.61%
NA
K14me1K18me3
0.02%
NA
K4me2K23me2
0.00%
NA
R8me2K36ac
0.02%
NA
K14me3K18me3
0.74%
K4me2K23me3
0.00%
NA
R8me2K36me2
1.73%
NA
K14me2K18me1
0.00%
0.01%
NA
0.02%
NA
K14me3K18me2
0.00%
0.01%
NA
0.01%
NA
K14me2K18me2
0.00%
0.01%
NA
0.01%
NA
K4me1R26me1
0.02%
0.02%
NA
R8me1K36ac
K4me1R26me2
0.02%
0.02%
NA
R8me1K37me2
K4me3R26me2
0.02%
NA
R8me2K37me1
0.01%
NA
K14me1K18me2
0.00%
0.01%
NA
K4me3R26me1
0.02%
NA
R8me1K37me3
0.01%
NA
K14me2K18ac
0.00%
0.01%
NA
0.00%
K4me1K27ac
0.02%
0.02%
NA
R8me2K37me3
0.02%
NA
K14acK18me2
0.38%
0.01%
NA
K4me1K27me1
0.02%
0.01%
NA
R8me2K37me2
0.01%
NA
K14acK23me3
0.46%
0.76%
NA
K4me1K27me2
4.94%
0.01%
NA
R8me2R40me2
0.02%
NA
K14me1K23me3
0.02%
0.01%
NA
K4me1K27me3
0.02%
0.02%
NA
R8me1R40me2
0.01%
NA
K14me3K23me3
0.02%
0.01%
NA
K4me3K27me1
0.02%
NA
R8me2R40me1
0.00%
NA
K14me3K23me1
0.00%
0.01%
NA
K4me3K27me2
0.02%
NA
R8me1R40me1
0.00%
NA
K14acK23me1
0.00%
0.01%
NA
K4me3K27ac
0.02%
NA
R8me1R42me1
0.00%
NA
K14me2K23me1
0.00%
0.01%
NA
K4me3K27me3
0.02%
NA
R8me2R42me2
0.01%
NA
K14me2K23ac
0.21%
0.28%
NA
K4me2K27me2
0.00%
NA
R8me1R42me2
0.01%
NA
K14acK23me2
0.01%
NA
K4me2K27me1
1.69%
NA
R8me1R49me1
0.00%
NA
K14me1K23me2
0.01%
NA
0.01%
0.01%
K4me1K36me1
0.02%
0.12%
NA
R8me2R49me2
0.01%
NA
K14me2K23me2
0.01%
NA
K4me1K36me2
4.94%
1.71%
NA
R8me1R49me2
0.01%
NA
K14me2K23me3
0.01%
NA
K4me1K36me3
0.02%
0.01%
NA
K9me1K14me2
0.00%
0.37%
NA
K14me3K23me2
0.01%
NA
K4me3K36me3
0.02%
NA
K9me2K14me2
0.00%
0.01%
NA
K14me3R26me2
0.01%
0.02%
NA
K4me3K36me2
2.95%
NA
K9me3K14me2
0.00%
0.01%
NA
K14me2R26me2
0.00%
0.02%
NA
K4me3K36me1
0.02%
NA
K9acK14me2
0.00%
0.47%
NA
K14me2R26me1
0.00%
0.01%
NA
K4me3K36ac
0.02%
NA
K9acK18me3
0.02%
NA
K14acK27ac
0.01%
0.02%
NA
K4me1K36ac
0.02%
NA
K9me1K18me3
0.02%
NA
K14me1K27ac
0.01%
1.10%
NA
32
Table S6 - continues
Wild type
Suz12-/-
t-test
K14me3K27ac
Binary co-existence
0.01%
0.02%
NA
K18me2K23me2
Binary co-existence
K14me2K27ac
0.21%
0.02%
NA
K18me3R26me1
K14me2K27me3
0.00%
0.01%
NA
K14me2K27me1
0.00%
0.70%
K14me2K27me2
0.00%
K14me2K36me1
Wild type
Suz12-/-
t-test
Suz12-/-
t-test
0.01%
NA
K23me1K37me3
Binary co-existence
Wild type
0.01%
NA
0.02%
NA
K23me3K37me2
0.01%
NA
K18me3R26me2
0.02%
NA
K23me2K37me2
0.01%
NA
NA
K18me2R26me2
0.00%
0.02%
NA
K23acR40me2
0.01%
NA
0.01%
NA
K18me2R26me1
0.00%
0.01%
NA
K23me3R40me2
0.01%
NA
0.00%
0.98%
NA
K18acK27ac
0.01%
0.02%
NA
K23me1R40me2
0.01%
NA
K14me2K36me2
0.00%
0.01%
NA
K18me1K27ac
0.01%
0.02%
NA
K23me2R40me2
0.01%
NA
K14me2K36me3
0.00%
0.01%
NA
K18me3K27ac
0.02%
NA
K23acR40me1
0.00%
NA
K14me2K36ac
0.02%
NA
K18me3K27me1
0.02%
NA
K23acR42me1
0.01%
1.35%
NA
K14me3K36ac
0.02%
NA
K18me3K27me2
0.74%
NA
K23me1R42me1
0.01%
0.00%
NA
K14acK36ac
0.02%
NA
K18me3K27me3
0.02%
NA
K23acR42me2
0.01%
0.14%
NA
K14me1K36ac
0.02%
NA
K18me2K27me2
0.00%
0.01%
NA
K23me3R42me2
0.01%
NA
K14acK37me2
0.00%
0.01%
NA
K18me2K27me3
0.00%
0.01%
NA
K23me2R42me2
0.01%
NA
K14me2K37me2
0.00%
0.01%
NA
K18me2K27me1
0.00%
0.01%
NA
K23me1R42me2
0.01%
NA
K14me1K37me2
0.00%
0.01%
NA
K18me2K27ac
0.00%
0.02%
NA
K23me2R42me1
0.00%
NA
K14me3K37me2
0.00%
0.00%
NA
K18me3K36me1
0.02%
NA
K23me3R42me1
0.00%
NA
K14me2K37me1
0.00%
0.46%
NA
K18me3K36me2
0.74%
NA
K23me3R49me2
0.02%
0.01%
NA
K14acK37me3
1.02%
NA
K18me3K36me3
0.02%
NA
K23acR49me1
0.38%
0.00%
NA
K14me2K37me3
0.01%
NA
K18me2K36me3
0.00%
0.01%
NA
K23me1R49me1
0.01%
0.00%
NA
K14me3K37me3
0.00%
NA
K18me2K36me2
0.00%
0.01%
NA
K23me2R49me2
0.01%
NA
K14me1K37me3
0.01%
NA
K18me2K36me1
0.38%
0.01%
NA
K23me1R49me2
0.95%
NA
K14me1R40me2
0.01%
NA
K18me2K36ac
0.02%
NA
K23me3R49me1
0.00%
NA
K14acR40me2
0.01%
NA
K18me1K36ac
0.02%
NA
K23me2R49me1
0.00%
NA
K14me3R40me2
0.01%
NA
K18acK36ac
0.02%
NA
R26me1K27ac
0.01%
0.02%
NA
K14me2R40me2
0.01%
NA
K18me3K37me1
0.02%
NA
R26me2K27ac
0.01%
0.02%
NA
K14me3R40me1
0.00%
NA
K18acK37me2
0.00%
0.22%
NA
R26me1K36ac
0.02%
NA
K14me1R40me1
0.00%
NA
K18me1K37me2
0.00%
0.01%
NA
R26me2K36ac
0.02%
NA
K14me2R40me1
0.00%
NA
K18me2K37me2
0.00%
0.01%
NA
R26me1K37me2
0.00%
0.01%
NA
K14acR40me1
0.00%
NA
K18me2K37me1
0.38%
0.01%
NA
R26me2K37me2
0.00%
0.02%
NA
K14me1R42me1
0.01%
0.00%
NA
K18acK37me3
1.10%
NA
R26me2K37me3
0.02%
NA
K14me1R42me2
0.01%
0.01%
NA
K18me2K37me3
0.01%
NA
R26me1K37me3
0.02%
NA
K14acR42me2
0.01%
0.01%
NA
K18me1K37me3
0.01%
NA
R26me1R40me2
0.02%
NA
K14me3R42me2
0.01%
0.01%
NA
K18acR40me2
0.01%
NA
R26me2R40me2
0.02%
NA
K14me2R42me2
0.01%
NA
K18me2R40me2
0.01%
NA
R26me1R40me1
0.00%
NA
K14acR42me1
0.00%
NA
K18me1R40me2
0.01%
NA
R26me2R42me1
0.01%
K14me2R42me1
0.00%
NA
K18me2R40me1
0.00%
NA
R26me1R42me1
0.01%
K14me3R42me1
0.00%
NA
K18acR40me1
0.00%
NA
R26me1R42me2
0.01%
0.00%
NA
K18me1R40me1
0.00%
NA
R26me2R42me2
K14me2R49me2
0.01%
NA
K18acR42me1
0.01%
0.00%
NA
R26me2R49me2
0.02%
K14acR49me1
0.00%
NA
K18me1R42me1
0.01%
0.00%
NA
R26me1R49me1
0.01%
K14me3R49me1
0.00%
NA
K18acR42me2
0.01%
0.01%
NA
R26me2R49me1
0.01%
K14me2R49me1
0.00%
NA
K18me1R42me2
0.01%
0.01%
NA
K27acK36me1
0.15%
0.02%
NA
K14me1R49me1
0.01%
NA
NA
0.01%
NA
0.02%
NA
0.02%
NA
NA
NA
R17me1K18me3
0.02%
NA
K18me2R42me2
0.01%
NA
K27acK36me2
0.01%
1.10%
NA
R17me2K18me3
0.02%
NA
K18me2R42me1
0.00%
NA
K27acK36me3
0.01%
0.02%
NA
R17me1K18me2
0.00%
0.01%
NA
K18me3R49me2
0.02%
NA
K27me1K36ac
0.02%
NA
0.01%
NA
K18me1R49me1
0.01%
0.00%
NA
K27me3K36ac
0.02%
NA
0.01%
0.00%
NA
K27me2K36ac
0.02%
NA
0.02%
NA
R17me2K18me2
R17me1K23me3
0.02%
0.01%
NA
K18acR49me1
R17me2K23me3
0.02%
0.01%
NA
K18me2R49me2
0.01%
NA
K27acK36ac
R17me2K23me1
0.01%
0.01%
NA
K18me2R49me1
0.00%
NA
K27acK37me1
0.01%
0.02%
NA
R17me2K23me2
0.01%
NA
K23me3R26me1
0.02%
0.02%
NA
K27me2K37me2
0.00%
0.01%
NA
R17me1K23me2
0.01%
NA
K23me3R26me2
0.02%
0.02%
NA
K27me1K37me2
0.00%
0.57%
NA
R17me1R26me1
0.97%
0.02%
NA
K23me2R26me1
0.02%
NA
K27acK37me2
0.00%
R17me1R26me2
0.01%
0.02%
NA
K23me2R26me2
0.02%
NA
K27me3K37me2
0.00%
R17me1K27ac
0.01%
0.02%
NA
K23acK27ac
0.95%
1.10%
NA
R17me2K27ac
0.01%
0.02%
NA
K23me3K27ac
0.02%
0.02%
R17me1K27me3
0.01%
0.02%
NA
K23me3K27me1
0.02%
R17me2K36ac
0.02%
NA
K23me3K27me2
R17me1K36ac
0.02%
NA
0.00%
R17me1K37me3
NA
0.01%
NA
K27me1K37me3
1.10%
NA
NA
K27me2K37me3
0.01%
NA
0.01%
NA
K27me3K37me3
0.02%
NA
0.02%
0.01%
NA
K27me2R40me2
0.01%
NA
K23me3K27me3
0.02%
0.02%
NA
K27me1R40me2
0.62%
NA
NA
K23me1K27ac
0.01%
0.02%
NA
K27me3R40me2
0.02%
NA
0.00%
NA
K23me2K27me1
0.01%
NA
K27acR40me2
0.02%
NA
R17me2K37me2
0.01%
NA
K23me2K27me2
0.01%
NA
K27me2R40me1
0.00%
NA
R17me2K37me3
0.01%
NA
K23me2K27ac
0.02%
NA
K27me3R40me1
0.00%
NA
R17me1R40me2
0.01%
NA
K23me2K27me3
0.02%
NA
K27me1R40me1
0.00%
NA
R17me2R40me2
0.01%
NA
K23me3K36me1
0.02%
0.01%
NA
K27acR42me1
0.01%
R17me1K37me2
0.00%
NA
R17me2R42me1
0.01%
0.00%
NA
K23me3K36me2
0.02%
0.01%
NA
K27me1R42me1
0.01%
0.00%
NA
R17me1R42me1
0.01%
0.00%
NA
K23me3K36me3
0.02%
0.01%
NA
K27me2R42me1
0.01%
0.00%
NA
R17me1R42me2
0.01%
0.01%
NA
K23me1K36ac
0.02%
NA
K27me3R42me1
0.01%
0.01%
NA
K23acK36ac
0.02%
NA
K27me2R42me2
0.01%
0.44%
NA
R17me2R42me2
NA
R17me2R49me2
0.02%
0.01%
NA
K23me3K36ac
0.02%
NA
K27me3R42me2
0.01%
0.01%
NA
R17me1R49me1
0.01%
0.00%
NA
K23me2K36me3
0.01%
NA
K27me1R42me2
0.01%
0.26%
NA
R17me2R49me1
0.01%
0.00%
NA
K23me2K36me2
0.01%
NA
K27acR42me2
0.01%
0.02%
NA
K18me3K23ac
0.74%
NA
K23me2K36me1
0.01%
NA
K27acR49me2
0.29%
0.02%
NA
K18acK23me3
0.02%
0.01%
NA
K23me2K36ac
0.02%
NA
K27acR49me1
0.38%
K18me1K23me3
0.02%
0.01%
NA
K23me3K37me1
0.02%
0.01%
NA
K27me1R49me1
0.01%
K18me3K23me3
0.02%
NA
K23acK37me2
0.31%
0.67%
NA
K27me3R49me1
0.43%
K18me2K23me1
0.00%
0.01%
NA
K23me1K37me2
0.00%
0.01%
NA
K27me2R49me1
0.01%
0.00%
NA
K18me2K23ac
0.38%
0.01%
NA
K23me2K37me1
0.01%
NA
K36me3K37me2
0.00%
0.01%
NA
K18acK23me2
0.01%
NA
K23me2K37me3
0.01%
NA
K36me1K37me2
0.00%
0.49%
NA
K18me1K23me2
0.01%
NA
K23acK37me3
0.01%
NA
K36me2K37me2
0.00%
0.01%
NA
K18me2K23me3
0.01%
NA
K23me3K37me3
0.01%
NA
K36acK37me1
0.02%
NA
33
NA
0.00%
NA
NA
Table S6 - continues
Suz12-/-
t-test
K36me2K37me3
Binary co-existence
Wild type
0.01%
NA
K36acR42me2
Binary co-existence
K36me3K37me3
1.02%
NA
K36me2R49me1
K36me1K37me3
1.10%
NA
K36me3R40me2
0.01%
K36me1R40me2
Wild type
Suz12-/-
t-test
0.02%
NA
K37me1R49me1
Binary co-existence
Wild type
Suz12-/-
t-test
0.01%
0.00%
0.01%
0.00%
NA
NA
K37me3R49me1
0.00%
K36me1R49me1
0.01%
0.00%
NA
NA
K37me2R49me1
0.00%
NA
K36me3R49me1
0.01%
NA
0.00%
NA
K37me2R49me2
0.01%
0.01%
NA
NA
K36acR49me2
0.02%
NA
K37me3R49me2
0.01%
K36me2R40me2
0.01%
NA
NA
K37me1R40me2
0.01%
NA
R40me2R42me2
0.01%
K36acR40me2
NA
0.02%
NA
K37me3R40me2
0.01%
NA
R40me2R42me1
0.00%
NA
K36me1R40me1
0.00%
NA
K37me2R40me2
0.01%
NA
R40me1R42me2
1.30%
NA
K36me2R40me1
0.00%
NA
K37me1R40me1
0.00%
NA
R40me2R49me2
0.01%
NA
K36me3R40me1
0.00%
NA
K37me2R40me1
0.00%
NA
R40me2R49me1
0.00%
NA
0.00%
NA
K36me2R42me1
0.01%
0.00%
NA
K37me1R42me1
0.01%
0.00%
NA
R40me1R49me2
K36me1R42me1
0.01%
0.00%
NA
K37me1R42me2
0.01%
0.01%
NA
R42me1R49me1
0.01%
0.00%
NA
K36me3R42me1
0.01%
0.00%
NA
K37me3R42me1
0.00%
NA
R42me2R49me2
0.01%
0.28%
NA
K36me3R42me2
0.01%
0.01%
NA
K37me2R42me2
0.01%
NA
R42me2R49me1
0.00%
NA
K36me1R42me2
0.01%
0.40%
NA
K37me2R42me1
0.00%
NA
R42me1R49me2
0.00%
NA
K36me2R42me2
1.58%
0.01%
NA
K37me3R42me2
0.01%
NA
34
Table S7 and S8
Table S7
Histone H4
Histone code
Table S8
Wild type
Suz12-/-
Wild type
Suz12-/-
2.32%
0.86%
S1ac
90.60%
91.41%
K5acK20me1
0.35%
2.08%
K5acK20me2
11.12%
6.28%
K5ac
5.86%
4.43%
K13me1
0.64%
B.D.L.
K5acK20me3
0.38%
0.17%
R3me1
0.57%
0.32%
S1ac
15.08%
15.46%
K5acK36me1
0.37%
0.14%
S1acK12ac
0.24%
0.39%
S1acK15me1
1.09%
2.32%
S1acK12acK20me2
1.73%
1.02%
K9me1
0.21%
B.D.L.
S1acK12acR23me2
0.26%
B.D.L.
(unmodified)
0.25%
B.D.L.
S1acK16ac
2.30%
2.26%
S1acR17me1
0.15%
0.33%
S1acK16acK20me1
0.53%
0.50%
S1acK5ac
0.13%
0.05%
S1acK16acK20me2
4.44%
8.34%
S1acK5acK15me1
0.05%
B.D.L.
S1acK20me1
9.21%
6.53%
S1acK9acR17me1
0.03%
B.D.L.
S1acK20me2
47.93%
50.38%
K5acK9ac
0.03%
B.D.L.
S1acK20me3
3.41%
3.18%
K5acR32me1
0.02%
B.D.L.
S1acR17me2
0.70%
B.D.L.
S1acK9ac
0.01%
B.D.L.
K5acR19me1K20me2
B.D.L.
0.09%
S1acK9acK15me1
0.00%
B.D.L.
S1acK16acR23me2
B.D.L.
1.12%
K5acR29me1
B.D.L.
0.15%
S1acK20me1R23me1
B.D.L.
0.54%
S1acR29me2
B.D.L.
0.13%
S1acK5ac
B.D.L.
0.11%
K5acR20me1
B.D.L.
0.07%
S1acK8ac
B.D.L.
0.05%
S1acK5acR17me1
B.D.L.
0.02%
S1acK8acK12ac
B.D.L.
0.15%
S1acK8acK20me2
B.D.L.
0.43%
S1acR23me2
B.D.L.
0.06%
K5ac
Histone code
Histone H2A
Table S9
Wild type - 1
in Histone
database
290
0
0.00%
Wild type - 2
323
0
0.00%
Wild type - 3
318
0
0.00%
Wild type - 4
307
0
0.00%
Suz12 - 1
300
0
0.00%
Suz12 - 2
298
0
0.00%
Suz12 - 3
327
0
0.00%
Suz12 - 4
293
0
0.00%
in Decoy
FDR
Above identity threshold
in Decoy
FDR
Wild type - 1
in Histone
database
293
0
0.00%
Wild type - 2
327
0
0.00%
Wild type - 3
323
0
0.00%
Wild type - 4
313
0
0.00%
Suz12 - 1
308
0
0.00%
Suz12 - 2
304
0
0.00%
Suz12 - 3
337
0
0.00%
Suz12 - 4
300
0
0.00%
above identity or homology threshold
35
Table S10
K27
K36
unmod
me1
me2
me3
ac
unmod
unmod
unmod
unmod
unmod
Jung et al.* Middle-down
Suz12-/WT
Suz12-/-
WT
Suz12-/WT
Suz12-/-/-
Suz12-/-
WT
Suz12-/-
unmod
me1
me2
me3
ac
me1
me1
me1
me1
me1
unmod
me1
me2
me3
ac
me2
me2
me2
me2
me2
unmod
me1
me2
me3
ac
me3
me3
me3
me3
me3
Suz12-/-
unmod
me1
me2
me3
ac
ac
ac
ac
ac
ac
Suz12-/-
Suz12
Suz12-/WT
WT
Suz12-/-
WT
Suz12-/-
Suz12-/WT
WT
WT
WT
WT
Suz12-/-
Suz12-/-
Suz12-/WT
WT
Suz12-/-
36
Table S11
K4me1
K4me2
K4me3
K4ac
R8me1
R8me2
K9me1
K9me2
K9me3
K9ac
K14me1
H3.2 wild type
2.13%
0.03%
0.13%
0.09%
5.08%
0.80%
H3.2 Suz12-/-
5.52%
0.13%
0.08%
0.00%
6.20%
0.57%
41.25%
5.09%
11.01%
3.32%
22.65%
30.87%
39.34%
6.16%
1.58%
H3.3 wild type
1.62%
0.00%
0.00%
0.00%
4.25%
16.27%
0.26%
19.19%
10.92%
11.64%
7.81%
8.24%
H3.3 Suz12-/-
2.65%
0.00%
0.00%
0.00%
1.09%
0.06%
17.26%
39.06%
22.36%
2.21%
9.99%
wild type (t-test)
27.59%
17.04%
8.14%
Suz12-/- (t-test)
1.38%
10.77%
17.04%
4.52%
33.77%
5.87%
0.01%
6.60%
40.31%
8.96%
0.00%
N.A.
0.00%
0.32%
0.01%
48.01%
1.00%
32.35%
1.14%
K14me2
K14me3
K14ac
R17me1
R17me2
K18me1
K18me2
K18me3
K18ac
K23me1
K23me2
H3.2 wild type
0.02%
0.13%
16.15%
1.52%
0.62%
3.96%
0.11%
0.01%
10.77%
1.04%
0.65%
H3.2 Suz12-/-
0.91%
0.36%
18.44%
1.17%
0.74%
2.81%
1.11%
0.07%
8.91%
0.65%
0.27%
H3.3 wild type
0.00%
0.85%
18.35%
0.83%
1.88%
4.79%
1.17%
0.59%
12.78%
0.83%
9.85%
H3.3 Suz12-/-
0.59%
4.96%
15.20%
1.81%
0.17%
4.33%
0.21%
0.61%
9.66%
2.27%
1.50%
wild type (t-test)
10.53%
10.45%
33.11%
21.05%
17.83%
30.35%
4.86%
0.95%
34.98%
33.21%
0.69%
Suz12-/- (t-test)
29.42%
3.93%
30.16%
20.77%
1.11%
24.33%
1.05%
7.51%
43.97%
7.03%
12.58%
K23me3
K23ac
R26me1
R26me2
K27me1
K27me2
K27me3
K27ac
K36me1
K36me2
K36me3
H3.2 wild type
0.86%
28.66%
2.25%
0.44%
6.38%
82.10%
5.26%
0.94%
9.12%
77.48%
2.34%
H3.2 Suz12-/-
0.24%
23.30%
1.54%
0.11%
19.77%
16.62%
1.40%
0.99%
24.32%
38.69%
7.73%
H3.3 wild type
10.87%
31.75%
2.75%
1.22%
6.56%
59.46%
18.79%
3.99%
11.12%
61.23%
3.44%
H3.3 Suz12-/-
6.38%
29.31%
1.19%
1.46%
16.30%
3.75%
3.20%
1.84%
28.99%
41.23%
6.83%
wild type (t-test)
1.85%
36.10%
38.20%
22.17%
45.34%
0.05%
0.48%
12.47%
12.54%
0.40%
24.68%
Suz12-/- (t-test)
2.70%
15.88%
29.98%
9.97%
20.18%
0.01%
13.00%
15.21%
16.53%
31.54%
34.06%
K36ac
K37me1
K37me2
K37me3
R40me1
R40me2
R42me1
R42me2
R49me1
R49me2
H3.2 wild type
0.26%
3.14%
0.01%
0.12%
0.19%
0.43%
0.15%
0.28%
0.01%
0.44%
H3.2 Suz12-/-
0.50%
11.97%
0.03%
0.17%
0.87%
5.38%
0.56%
1.23%
0.16%
1.09%
H3.3 wild type
0.37%
2.71%
0.00%
0.15%
0.23%
1.02%
0.20%
0.13%
0.00%
0.26%
H3.3 Suz12-/-
1.88%
8.11%
0.04%
0.30%
0.10%
0.27%
0.14%
0.68%
0.04%
0.80%
wild type (t-test)
38.81%
32.81%
9.51%
42.21%
39.74%
28.62%
35.69%
14.36%
17.04%
11.02%
Suz12-/- (t-test)
3.69%
4.65%
45.40%
33.66%
2.61%
0.00%
8.63%
9.97%
9.87%
34.90%
37