SAMPLING AND SHIPMENT INSTRUCTIONS FOR MALARIA PCR TEST The PCR can be done directly from EDTA whole blood or from EDTA blood cells spotted on Whatman No 4 filter paper EDTA whole blood: a minimum volume of 2 ml EDTA blood are required Filter paper: 1) Do not use heparin 2) Put identification number on one end of filter paper (Whatmann filter paper # 4). 3) Place a drop of whole blood (40 ul) on the filter at the opposite end to the identification number 4) Testing can be also done on plasma/serum or CSF. 5) Send up to 4 well-separated blood spots on a single sheet 6) Let the sample dry thoroughly at room temperature 7) If sending more then 1 sample, place clean filter paper between each sample 8) Place the filter papers in a plastic bag with silica gel and store at room temperature 10) Samples can be sent at room temperature If shipping to Canada from another country, attach; 1) shipper's declaration for dangerous goods 2) commercial invoice 3) Fedex expanded service international air-way bill form. Ship to Momar Ndao DVM, PhD/Lab Director National Reference Centre for Parasitology Montreal General Hospital 1625 Pine Ave. West Montreal, QC H3G 1A4 Phone: 514-934-8347