BIOHEALTH COMPUTING
MASTER THESIS JOINT RESEARCH PROJECT FORM
First name
ZEYNEP
Last name
AYDOĞMUŞ
Status
Prof. Dr.
Institution or company
Istanbul University, Faculty of Pharmacy, Department of
Analytical Chemistry
Laboratory
Department of Analytical Chemistry
Adress 1
Istanbul University, Faculty of Pharmacy, Department of
Analytical Chemistry, 34116 Beyazıt, Istanbul, Turkey
Adress 2
Zip Code
34116
City
Istanbul
Country
Turkey
Email
aydogmus@istanbul.edu.tr /zaydogmus@yahoo.com
Phone number
+902124400000-13591
Fax
+902124400252
Partner's or associate partner's Area
Major track
Clinical
Minor track
Biotech
Thesis Title *
Development new electrochemical techniques for
analysis of benzodiazepines in biological specimens
Research probleme:
Benzodiazepines (BZDs) with similar chemical structures used in the treatment of anxiety, insomnia,
agitation, epilepsy, muscle spasms, alcohol withdrawal and other psycho-physiologic disorders. They are
also used nausea and vomiting associated with emetogenic cancer chemotherapy. BZDs have different
absorption and pharmacological parameters. BZDs are relatively safe drugs. However, most
benzodiazepines have long elimination half-lives and can accumulate particularly in elderly patients in
long time medication. Benzodiazepines have high potential for abuse and misuse. Also, BZDs may
replace barbiturates and other sedative drugs and affect daily live. BZDs have been administered in
various therapeutic doses a day. Low dose administrations need sensitive assays. In literature, generally
BZDs have been determined by HPLC-UV and HPLC-mass methods. For all those of reason,
individualizing therapy and monitoring of BZDs concentration in biological specimens, development of
more sensitive, simple and robust methods are necessary.
Objectives:
The goal of this project is to develop new powerful electrochemical techniques/methods and electrosensors for analysis of benzodiazepines alone and/or in mixture of drugs of this group and their
metabolites, and apply in biological specimens such as serum, plasma urine. The developed techniques
will also apply to investigate drug interaction in respect of different benzodiazepine structures in mixture
and related other drugs and compounds.
Work program :
Developed and validation of new electrochemical techniques such as voltammetry, amperometry, liquid
chromatography coupled electrochemical detector, and also develop chemical sensor to use in these
techniques will be carried out in our laboratory. In order to apply developed techniques/methods to in
vitro and in vivo biological samples and recruiting the volunteers, we will collaborate with Istanbul
University hospitals. The second part of this project will be application of the developed methods to drugdrug interaction studies. This will be studied mainly in our laboratory and in collaboration with relevant
departments of Istanbul University hospitals.
Keyword 1 *
Benzodiazepines
Keyword 2 *
Electrochemistry
Keyword 3 *
Biological fluids
Keyword 4
Therapeutic monitoring
Keyword 5
Determination
Major track *
 Molecular Biotechnology / Biophysics Nanotech
 Clinical Research
 Environment Health
 Computational Mathematics