Course Outline Format
Advanced Analytical Chemistry CH-507
MS CHE
Program
3
Credit Hours
One Semester
Duration
3 hours/week
Graduate Standing
Prerequisites
Resource Person
Counseling Timing
Contact
Chairman/Director Programme signature………………. Dean’s signature…………
Date………………………………….
Learning Objective
Credit Hours:3
Resource Person:
Semester:Spring 2015
Objective:
The course objective is to introduce the participants to modern instrumental techniques and analytical
approaches. After completing the course the student should be able to:



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Learn about sample extraction and sample preparation techniques.
Develop and validate methods for separating compounds and determine their concentration.
Identify unknown compounds by modern instrumental techniques.
Evaluate and discuss analytical chemical data.
 Do problem solving in analytical chemistry
Syllabus:
Described in lecture schedule
Learning Methodology
Grade Evaluation Criteria
Marks Evaluation
Marks in percentage
Quizzes+Assignments
20%
Mid Term
25%
Attendance & Class Participation
0%
Term Project
10%
Presentations
10%
Final exam
35%
Total
100%
Recommended Books
 Christian, G.D.,Analytical Chemistry,6th ed., John-Wiley & Sons, (2004).
 Skoog, D.A., West, P.M., Holler, F.J. and Crouch, S. R., Fundamentals of Analytical
Chemistry, 8th ed., Brooks/Cole Nelson. (2007)
 Harvey, D., Modern Analytical Chemistry, 2nd ed., McGraw Hill Ltd., (2009)
Reference Books
• Grob, R. L., Eugene, F. Barry, Modern Practice of Gas Chromatography, 4th ed., John-Wiley
& Sons, USA, (2004).
• Lindsay, S., High Performance Liquid Chromatography, 2nd ed., John-Wiley & Sons, Ltd.,
(1992).
• Sharma, B.K. Instrumental Methods of Chemical Analysis, 24th ed., Goel Publishing House,
Meerut, India, (2005).
Calendar of Course contents to be covered during semester
Week
TOPICS
1
Introduction to analytical chemistry: Choice of analytical methodology, Errors,
Accuracy and precision, Average derivation and standard derivation, variance and
confidence limit, Significance figures and computation rules
2
Extraction methods: Liquid-liquid extraction, Solid phase extraction, Super-critical
fluid extraction and Accelerated solvent extraction, Fractionation methods.
3
Methods of sampling: Samples size. Techniques of sampling, Sample preparation,
Chemical analysis, Tools for quantitative chemical analysis, Quality assurance.
4
Chromatographic Methods: General principle, Classification, Nature of partition
forces, Column efficiency and resolution.
5
Thin Layer Chromatography (TLC): Coating of materials, Preparative TLC. Solvents
used and methods of detection, Applications.
6
Column Chromatography: Adsorption and Partition methods, Nature of column
materials, Preparation of the column, Solvent systems and Detection, Applications.
7
High Performance Liquid Chromatography (HPLC): Sample injection, Nature of
columns, Solvent systems and Detectors, Optimization, Use of standards, Liquid
Chromatography Mass Spectrometry (LC-MS), Applications.
8
Gas Chromatography: Sample injection, Nature of columns, Optimization of
experimental conditions, Gas Solvents, Columns, Gas Chromatography Mass
Spectrometry (GC-MS), Applications.
9
MID TERM
10
Mass Spectrometer: Principle, Working, Formation of different types of Ions,
Fragmentation of organic compounds, Applications.
11
Ultraviolet and Visible spectrophotometry (UV-VIS): Beer Lambert’s law,
Instrumentation, Calculation of absorption maxima, Applications.
12
Infrared Spectroscopy (IR): Principle, instrumentation, sampling technique, selection
rules, absorption of common functional groups. Factors affecting frequencies,
Applications
13
Capillary Electrophoresis (CE): Electrophoresis, Theory and principle of CE,
mobility, electro-osmotic flow separation by CE, instrumentation, modes of
operation, Applications
14
Flame Emission Spectroscopy (FES): Principle, Instrumentation, Distribution
between Ground and Excited States, Applications.
15
Atomic Absorption Spectroscopy (AAS): Principle, Instrumentation, Single and
double beam AAS, detection limit and sensitivity, Interferences, Applications.