INTRODUCTION
Instrumental Analysis &
Separations
FOS 6355
TOPICS
There are basically two types of analysis:
 Qualitative - What is in the sample?
 Quantitative - How much is in the sample?

TOPICS

This course will be focusing on Quantitative
Analysis.

Qualitative
infrared spectroscopy
 NMR
 Mass Spectrometry

TOPICS

This course will focus almost exclusively on
chemical analysis and separations using
chromatography.
HPLC - High Performance Liquid Chromatography
 GC - Gas Chromatography

GRADING POLICY
 Three
Exams (3 x 150 pts……….450 (45%)
 Three
Papers (4 x 25 pts)……….100 (10%)
 Homework
(2 x 25 pts)...................50 (5%)
 Lab
Notebook &
Lab Reports (9)…………………..400 (40%)
 TOTAL……………………………1000
(100%)
PAPERS
You will need to search for a research article on the
four subject areas we will be studying.
 Extraction/separation (microwave assisted
extraction; supercritical extraction, ultrasound
extraction, etc.)
 Spectroscopy (Raman, IR, NMR, etc.)
 Chromatography
 Gas
 Liquid
 You will select an article relating the topic to food
science and write no more than a two page summary
of the article. Note, the article does not have to be
entirely about the subject but it should be majorly
used as the analytical tool in the article.

LECTURE AND LAB TIMES
Lecture will be from 11 am to 12:30 pm on
Tuesdays and Thursdays
 For the the first few weeks of the semester, lecture
will be extended and we will not meet for lab.

http://lss.at.ufl.edu
http://fshn.ifas.ufl.edu/faculty/mrmarshall/
LECTURE AND LAB TIMES
 Labs
are scheduled for Tuesday and
Thursday from 12:30 pm and going until
5 pm. However, labs may take more on
less time than the scheduled time.
 Not all groups will be able to perform
certain labs during the regularly
scheduled times, therefore, it will be
necessary for some groups to schedule
alternate lab hours for some
experiments.
LAB REPORTS & HOMEWORK
Formal lab reports are required
 This means that they must be type-written and
follow the specified format
 Homework may be hand-written

LAB NOTEBOOKS
1.
2.
3.
All entries will be written in blue or black
ink
Errors will be crossed out with a single line
and the correct entry added next to the
crossed out entry
Reserve the first 3 pages for the table of
contents
•
4.
Keep the table of contents up to date
Each page in your notebook will be signed
and dated by you after entries on that page
are completed.
LAB NOTEBOOKS (CONT.)
5.
6.
7.
8.
If you wish to begin a new section, insert a large
table, or begin a new experiment on a new page
without completely filling the previous page, make
a diagonal line across the blank section of the
page
Chromatograms or other computer output should
be taped into the notebook in an appropriate place
Lab partners need to insert the required
chromatographs
Have your lab partner sign your notebook after an
experiment is completed