SHARAF ABDOALLAH – EGITTO Course: Management and utilization of food resources aimed at the sustainability of agriculture and forestry, and at the mitigation of the effects of climate change Tipology : Graduate General information First name: Abdoallah Last name: Sharaf Gender: M Date of birth: 6/28/1982 Place of birth: Cairo Country of birth: Egypt Citizenship: Egyptian View ID document Residence Country of residence: Egypt City: Cairo Address: 40 elaqba St., from harun elrashed, Heliopolis Postal Code: 11351 Contact info Official e-mail (the same one used to register to the online application portal) abdoallah.sharaf@agr.asu.edu.eg Telephone (+ country code) 201110410111 Mobile phone 201110410111 Master's degree Field of degree: Agricultural Sciences Name of University/Institution: Ain Shams University City: Cairo Country: Egypt Graduation Date(MM/DD/YYYY): 4/7/2008 Degree Certificate: Master.pdf PhD PhD denomination: Genetics Field/s: Agricultural Sciences Name of University/Institution: Ain Shams University City: Cairo Country: Egypt Degree - issue of certificate - date (YEAR): 2012 Title of PhD: Molecular Genetic Identification of Induced Variants in Milk thistle (Silybum marianum L.) PhD certificate: PhD.pdf Post graduate courses Post graduate denomination: Post doctor Field/s: Genetics Name of University/Institution: University of Cordoba (UCO) City: Cordoba Country: Spain Post graduate - issue of certificate - date (YEAR): 2012 Main topics: - As a Member of Improving, transference and applicability of knowledge in conservation and characterization technologies in cattle breeds from Egypt and the Iberian Peninsula(CATTLECON IKTA a IRSES) Project. - Using molecular techniques to the study of populations.; Reconstruction of phylogenetic relationships.; Study and control of animal genetic variability.; Study of candidate genes.; Traceability of animal products.; Identification of genotype for coat color in the equine species.; Molecular identification of sex in birds or in cases of hermaphroditism and assigning individuals to racial entity. Post graduate denomination: Post doctor Field/s: Genetics Name of University/Institution: Centre for Research in Agricultural Genomics (CRAG), University of barcelona (UAB) City: Barcelona Country: Spain Post graduate - issue of certificate - date (YEAR): 2013 Main topics: - The main research activities of this group QTL characterization and gene discovery of characters of economical interest in domestic animals, Structural and functional genomics of porcine and ruminant species, Host-pathogen interaction in the resistance/susceptibility to infectious diseases in farm animals and Development of statistical and computational tools in animal genomics. Also I was integrated in some projects like “Study of the genetic predisposition and the genomic functional changes underlying cutaneous melanoma in a pig commercial population” “MelanoPig”, “the genetic Basis of NIFD and FALs phenotypes” and “in-silico discovery of new nonredundant Taste Receptor (TAS) genes within Pig Genome”. Working/Management experience Present/Past Position: 1 Job profile: Assistant Professor From (Year): 2012 To (Year): 2014 Name Institution/Employer: Ain Shams University Department/Institute/CompanyBiotechnology "Genetics" Country: Egypt Address: Hadaeq Shubra City: Cairo Postal code: 11241 Publications Type: Book(chapters of book) Source references : Authors, Title, Date of Publication and Publisher.: Abdoallah A. Sharaf, Molecular Genetic Characterization of Silybum Marianum, LAP LAMBERT Academic Publishing, ISBN-13: 978-3-659-50225-5, ISBN-10: 3659502251 and EAN: 9783659502255 Short Abstract: In this book we present a study aimed to determine the morphological, biochemical (protein SDS-PAGE and isozyme) and molecular (RAPD- and ISSR-PCR) characteristics in different ecological types of milk thistle collected from Egyptian habitats and identify levels of active ingredients as a way towards improvement of this plant on the commercial leve. the results of SDS-PAGE data for twelve silybum accessions confirmed that there were negative markers. Also, the results of RAPD and ISSR-PCR data analysis confirm that there were two positive markers. Finally both positive and negative marker for the highest production of the fixed oil by primers HB12 and X06 and three positive markers the lowest production of the fixed oil by primers HB14 and HB15. Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: - Abdoallah A. Sharaf, Ahmed Bahieldin, Samir A. Ibrahim, A.Z. E. Abdelsalam and Ashraf A.Khalil (2010) Biochemical and Genetic Characterization of 12 Silybum marianum Accessions Collected from Borg El-Arab, an Egyptian Habitat, Functional Plant Science and Biotechnology, 5 (Special Issue 1): 22-29. Short Abstract: Silybum marianum, known as milk thistle (MT), a plant that grows throughout the Mediterranean, southern Europe and parts of the US, has been used for some 2000 years as both food and medicine. The aims of this study were to determine the morphological, biochemical (protein profile and isozyme) and molecular (RAPD- and ISSR-PCR) characteristics of MT and to identify and quantify its active ingredients. Twelve accessions of MT collected from Borg El-Arab, an Egyptian habitat located at north/west cost, were tested. SDS-PAGE electrophoresis indicated that both accessions belong to location 3 and cultivated had specific bands. Data analysis of isozyme banding patterns showed different migration rates for four isozymes (±- and ²-esterase, peroxidase and acid phosphatase). These isozymes belonging to different Silybum accessions found in the locations may represent a degree of genetic variation. RAPD-PCR fingerprinting resulted in 83 amplicons (DNA bands), 33 of which were polymorphic. Furthermore, 10 accessions proved to have specific molecular markers. ISSR-PCR fingerprinting resulted in 40 amplicons, 16 of which were polymorphic. Six accessions-specific markers were found for MT accessions. The estimation of genetic distance based on SDS-PAGE, isozymes, RAPD-PCR and ISSR-PCR ranged from 85 to 95%. Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Delgado J.V., Gómez M., Landi V., Martínez A., Elbeltagi A., Sharaf A., Agha S., El-Saied S,Galal S. (2012) INTEGRATING LOCAL EGYPTIAN CATTLE BREEDS IN A NATIONAL BREEDING STRATEGY. A CATTLECONIKTA PROPOSAL, the VIII Iberian Congress on Animal Genetic Resources SPREGA SERGA, Évora, Portugal. 13 – 15 Sep. 2012. Short Abstract: Local Egyptian cattle have long supported farmers in producing animal products as well as providing animal power needed for their fields. These animals have shown along the centuries the best characteristics of adaptation to the Egyptian climatic conditions, tolerance to endemic diseases and producing and reproducing under less than optimum feeding conditions. These cattle have received little support, however, in term of genetic improvement and there has not been any directional selection program. There is also lack of well coordinated national breeding strategy for them. With growing demand on animal products the cattle have been used in indiscriminate crossing with exotics to produce better performing crossbreds. This has led to in some regions in the country to the scarcity of pure bred local cattle, hence the genetic integrity of the breed is undermined. Local cattle in Egypt are called under the umbrella name ‘Baladi’ literally meaning local, without any subdivision into breeds or other genetic subdivisions. Sometime they are named according to the region of the country they come from. A first approach for improving these cattle is to genetically characterize them at the genome level which also could expedite the selection effort as compared to the conventional selection using the quantitative genetics approach only. The country has got competent manpower and good technological resources to develop a successful program of livestock development based on the use of own re Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Molina-Flores B., Martinez A., Agha S., Sharaf A., Landi V., Galal S. and Delgado J. Genetic characterization of Egyptian Cattle population using Microsatellites Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Abdoallah A. Sharaf, Samir A. Ibrahim, A.Z. E. Abdelsalam. Molecular Identification of induced variants from Milk thistle (Silybum marianum). Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Amal I. Khalil, Gazaa H. Morsy, Gehan H. Lashein, Dina I. Abd El- Mottaleb and Abdoallah A.Sharaf. Molecular Variations Based on RAPD among Oxyurids of Rodents in Egypt Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Delgado J.V., Gómez M., Landi V., Martínez A., Elbeltagi A., Sharaf A., Agha S., El-Saied S,Galal S. Genetic characterization of Egyptian Goats population using Microsatellites. Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Delgado J.V., Gómez M., Landi V., Martínez A., Elbeltagi A., Sharaf A., Agha S., El-Saied S,Galal S. Genetic characterization of Egyptian Sheep population using Microsatellites. Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Clop A., Sharaf A. Study the genetic basis of NIFD and FALs phenotypes. Short Abstract: (In preparation) Type: Papers Source references : Authors, Title, Date of Publication and Publisher.: Sanchez A., Clop A., Sharaf A. discovery new nonredundant Taste Receptor (TAS) genes within Pig Genome. Short Abstract: (In preparation) Mother tongue: Arabic English level: excelent Other languages Spanish: very good French: good Computer skills ICDL, SPSS and MATLAB® Certificated. Operating systems: Dos, Widows, Mac. and LUNIX. Programming languages: R programming language, Python. Data Analysis Programs: SAS, SPSS, MATLAB® and R Studio. Online Programs: MEGA 3.1, MEGA 4 www.megasoftware.net/mega.html, Arlequin,cmpg.unibe.ch/software/arlequin3/ BLAST http://expasy.org/tools/blast/ and all tools in NCBI databases (http://www.ncbi.nlm.nih.gov/), Ensemble Genome Browser (http://www.ensembl.org/index.html) and UCSC Genome Browser Additional information informations Referee's contact details First letter of recommendation First name: Salah Last name: Galal University/Institution: Ain Shams University City: Cairo Country: Egypt E-mail: sgalal@tedata.net.eg Referee's contact details Second letter of recommendation First name: Juan Last name: Bermejo University/Institution: University of Córdoba City: Cordoba Country: Spain E-mail: juanviagr218@gmail.com MODULO DI VALUTAZIONE CORSO: Management and utilization of food resources aimed at the sustainability of agriculture and forestry, and at the mitigation of the effects of climate change Proponente: Sharaf Abdoallah Paese: Egypt Valutazione Education: 6.0 Working/management experience:6.0 Publications:7.0 Skills and competences:5.0 Letter of references:2.0 Punteggio totale 26.0