Supplementary Figure 1: Transmission electron micrograph of AgNPs (A) and Bulk Ag (B).
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A
B
Supplementary Figure 2: Images displaying the growth of soybean (A) and wheat (B) in the
growth tubes in a growth cart.
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Supplementary Figure 3: Light microscopy images of soybean root tips. (A) Control, (B)
AgNPs, (C) Ag+, and (D) Bulk Ag. Images above were taken are at 100X magnification.
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Supplementary Figure 4: Average shoot elongation rate (A), root elongation rate (B), and
transpiration rate (C) of soybean. The reported values are the average of ten replicates for each
treatment. Error bars represent standard deviation and letters above the error bars indicate the
homogeneous subset grouping determined using one-way ANOVA analysis with post-hoc Tukey
HSD test.
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Supplementary Figure 5: Light Microscopy images of wheat root tips. (A) Control, (B) AgNPs,
(C) Ag+, and (D) Bulk Ag. Images above were taken are at 100X magnification.
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Supplementary Figure 6: Average shoot elongation rate (A), root elongation rate (B), and
transpiration rate (C) of wheat. The reported values are the average of five replicates for each
treatment. Error bars represent standard deviation and letters above the error bars indicate the
homogeneous subset grouping determined using one-way ANOVA analysis with post-hoc Tukey
HSD test.
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Supplementary Figure 7: Scanning electron microscopy images of wheat root tips. (A)
secondary electron micrograph of control root tip, (B), (C), and (D) backscattered electron
micrograph of AgNPs, Ag+ and Bulk Ag, respectively.
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Plant Biomass Measurement
For the measurement of plant seedling biomass, individual seedling was carefully taken
out of the growing tube and gently placed on a clean sheet of thick paper towel at each weighing
event. Clean Kimwipes were used to delicately tap dry excess water from plant root surface. The
seedling was then immediately weighed using an Ohaus Pioneer PA153 Precision Balance
(OHAUS Corporation, Parsippany, NJ) and returned back to the growing tubes afterwards. These
steps were repeated for each seedling and only one seedling was taken out for measurement at
any time. New paper towels and kimwipes were used for each seedling to avoid cross
contamination.
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