Journal of Inclusion Phenomena and Macrocyclic Chemistry Supporting Information for Synthesis, characterization, spectrophotometric and electrochemistry studies, and DNA cleavage of copper(II) complexes of a new azacrown bis-macrocycle and its mono-cyclic analogue Zahra Kalantari Khoramdareha, Seyed Abolfazl Hosseini-Yazdia,*, Bernhard Spinglerb, Ali Akbar Khandara a Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 51666-14766 Iran b University of Zurich, Winterthurerstr. 190, 8057 Zürich, Switzerland *Corresponding author: Department of Inorganic Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 51666-14766, Iran, Tel.: +98-411-3393134; fax: +98-411-3340191. E-mail address: hosseiniyazdi@tabrizu.ac.ir or hosseiniyazdi@yahoo.com Fig. S1. ESI-MS spectra of compound 4, [M + Na] + molecular ion appeared at m/z = 1467 Fig. S2. 1H-NMR spectrum of 5 in CDCl3 Fig. S3. 13C-NMR spectrum of 5 in CDCl3 Fig. S4. 13C{DEPT}-NMR spectrum of 5 in CDCl3, show 6 aliphatic CH2 groups and aliphatic CH group and 8 aromatic carbon groups that completely confirmed bis-macrocycle structure Fig. S5. ESI-MS spectra of compound 5, [M + H+] and [M + 2H]2+ molecular ions appeared at m/z = 845 and 423 Fig. S6. 1H-NMR spectrum of 7 in CDCl3 Fig. S7. 13C{1H} NMR spectrum of 7 in CDCl3 Fig. S8. Observed distribution MALDI-MS spectrum of complex 8, [5 + Cu]+ and [5 + Cu + O]+ molecular ions appeared at m/z = 907 and 923 confirmed the copper complex Fig. S9. Job plot for complexation of 5 with Cu(NO3)2.3H2O. Solutions of 5 (1.25 × 10–3 M) and Cu(NO3)2.3H2O (1.25 × 10–3 M) were mixed in varying volume ratios in such a way that the total volume of each mixture was the same. Fig. S10. Agarose gel electrophoresis patterns for the cleavage of pGS2 plasmid DNA (650 ng/µL) by Cu(NO3)2.3H2O in the presence of 200-fold excess of MPA in the dark for 16 h in cacodylate buffer (0.1 mM, pH 7.5), NaCl (50 mM), MPA (20 mM) at lane1 (0 mM), lane 2 (0.1 mM), lane 3 (0.2 mM), lane 4 (0.3 mM), lane 5 (1 mM), lane 6 (2 mM) and lane 7 (5 mM) Cu(NO3)2.3H2O