Biol 1020
Lab 2 - Biochemistry
Lab Review
Question 1

Which of the following would be found
in starch?
6
CH2OH
6
O
5
OH
B
4
OH
H
H
1
H
HO
3
H
H
A
4
O
5
H
H
CH2OH
OH
H
OH
H
HO
3
2
1
H
2
OH
OH
Question 1

Which of the following would be found
in starch?
This is a Glucose
6
CH2OH
6
O
5
OH
B
4
OH
H
H
1
H
HO
3
H
H
A
4
O
5
H
H
CH2OH
OH
H
OH
H
HO
3
2
1
H
2
OH
OH
Question 2

If the 2 glucose's below were to join
where and how would the bond form?
6
CH2OH
5
6
CH2OH
O
5
H
H
O
H
H
H
H
4
4
1
1
OH
H
HO
3
OH
OH
2
3
H
H
HO
OH
H
2
OH
OH
Question 2

The bond would form between the 1st
and 4th carbon. It would involve the
removal of an OH and a H.
6
CH2OH
6
O
5
5
H
H
H
O
H
H
H
A
4
CH2OH
4
1
OH
H
HO
3
H
2
1
OH
OH
3
OH
H
HO
H
2
OH
OH
Question 2

The bond would form between the 1st
and 4th carbon. It would involve the
removal of an OH and a H.
6
CH2OH
6
CH2OH
O
5
H
H
H
H
H
H
A
4
O
1
HO
OH
1
H
OH
H
H
OH
O
3
H
OH
A new a 1-4 bond
OH
Question 3

What is this type of reaction called?
6
CH2OH
6
CH2OH
O
5
H
H
H
H
H
H
A
4
O
1
HO
OH
1
H
OH
H
H
OH
O
3
H
OH
A new a 1-4 bond
OH
Question 3

The reaction is a condensation reaction and
involves the removal of water (H2O).
6
CH2OH
6
CH2OH
O
5
H
H
H
H
H
H
A
4
O
1
HO
OH
1
H
OH
H
H
OH
O
3
H
OH
A new a 1-4 bond
OH
Question 4

Is glucose in this form a reducing
sugar?
Question 4

No- The double bonded Oxygen is part of
the ring. We need to heat the solution to
open the ring for the Benedicts test to be
positive.
Question 5
Which of the Catechol temperatures
reacted fastest?
A. Ice bath
B. Room Temp
C. 100ºC
D.
40ºC

Question 5
Which of the Catechol temperatures
reacted fastest?
A. Ice bath
B. Room Temp
C. 100ºC

D.
40ºC
Question 6

Why did the 100ºC Catechol not react?
A.The catechol bonds were broken by
the heat
B.The catachol oxidase bonds were
broken by the heat
C. The catachol oxidase was denatured
by the heat
D. The catechol was denatured by the
heat
Question 6
Why did the 100ºC Catechol not react?
A.The catechol bonds were broken by the
heat
B.The catachol oxidase bonds were
broken by the heat

C. The catachol oxidase was
denatured by the heat
D. The catechol was denatured by the
heat
Question 7
Why did the 0ºC Catechol not react?
A.The catechol was destroyed by the ice
B.There was not enough energy for the
reaction to occur
C. The potato was frozen
D. The catechol oxidase was destroyed
by the ice

Question 7
Why did the 0ºC Catechol not react?
A.The catechol was destroyed by the ice

B.There was not enough energy
for the reaction to occur
C. The potato was frozen
D. The catechol oxidase was destroyed
by the ice
Question 8

Which of the following would you think are
non- polar molecules based on the ability to
mix with oil?
A. Ether
B. Water
C. Soap
Question 8

Which of the following would you think are
non-polar molecules based on the ability
to mix with oil?
A. Ether
B. Water
C. Soap
The soap did not mix-it emulsified
Question 9
Which of the following is a difference
between a fat and a phospholipid?
A. fats have 3 fatty acids and phospholipids
have 2
B. fats have non polar heads and
phospholipids have polar heads
C. fats are used as an energy store while
phospholipids form bilayers (membranes)
D. fats usually contain only H,C,O while
phospholipid also contain N and P

Question 9
Which of the following is a difference
between a fat and a phospholipid?
A. fats have 3 fatty acids and phospholipids
have 2
B. fats have non polar heads and
phospholipids have polar heads
C. fats are used as an energy store while
phospholipids form bilayers (membranes)
D. fats usually contain only H,C,O while
phospholipid also contain N and P

All are true
Lab 2 - Biochemistry
Your lab bench must be cleaned
up before you leave.
Place all of the test tubes back in
the rack!
Leave all material in its original
location
Biol 1020
Lab 2 - Biochemistry
Lab Review