The Department of Biochemistry of Medical Faculty Presents now MOLECULAR CHAPERONES Edited by Attila Sandor one dimensional information L. Fig.3-16, p.89 three dimensional information The model proteins for classic study of developing the three dimensional structure, that for folding. (Anfinsen) Str., Fig. 7.1, p.172 Str. Fig. 3.53, p. 65 Scrambled Str. Fig. 3.54, p. 66 The three dimensional structure is dictated entirely by the amino acid sequence Str., Fig1.6 p.7 Free energy L.Fig.4-29,p. 149 Str. Fig. 3.57, p. 6+8 Role of chaperones in the thermodynamic point of view (a) The folding of the unfolded protein (U) to the correct (C) form is favored thermodynamically. Chaperone is not necessary. (b) The folding of the unfolded protein (U) to the incorrect (I) form is favored thermodynamically. Chaperone is necessary. Ellis R.J., Annu. Rev. Biochem., 1991 The idea of molecular chaperones Named after the human chaperone: “usually an elderly woman who accompanies a young unmarried lady to prevent not proper interactions with other people. Molecular chaperones: proteins assisting folding of nascent polypeptides, by preventing wrong folding. Molecular chaperones catalyze the formation of correctly folded, functionally active, native proteins, but they are not part of the product. Expression of many chaperon is induced by stress, such as by heat, because during heat-stress the probability of wrong folding is higher, therefore cells need more protection. These chaperons are called heat shock proteins (HSP’s). Author`s slide Representative members of the Chaperone family Nucleoplasmins Nucloplasmin NucleoplasminS Chaperonins chaperonin 60, groEL chaperonin 60, groEL chaperonin 10, groES (mitochondrial, bacterial) may use ATP Ellis R.J., Annu. Rev. Biochem., 1991 Heat shock proteins 70 ( Hsp70) Heat shock proteins 90 (Hsp90) Role of nucleoplasmines: assably of chromatin DNA + Histone DNA+ Nucleoplasmin + Histone aggregate Nucleosoma (DNA + Histone) Nucleoplasmin Author`s picture Representative members of the Chaperone family Nucleoplasmins Nucloplasmin NucleoplasminS Chaperonins chaperonin 60, groEL chaperonin 60, groEL chaperonin 10, groES (mitochondrial, bacterial) may use ATP Ellis R.J., Annu. Rev. Biochem., 1991 Heat shock proteins 70 ( Hsp70) Heat shock proteins 90 (Hsp90) RUBISCO and the chaperonins When removing guanine o Rubisco denaturated with guanine at +25 C very poor recovery o Rubisco denaturated with guanine at +10 C good recovery o Rubisco denaturated with guanine at +25 C and chaperonin 60, chaperonin 10, ATP, Mg++ were added Rubisco: ribulose 1,5-bisphosphate carboxylase-oxygenase Author`s slide 80% recovery L.Fig.41-31, p.152 THANK YOU FOR YOUR ATTENTION The honored audience has the opportunity now to download the pictures of this lecture c:\sandor\chaperon.ppt Attila Sandor