1.6: Stop-Motion Mitosis Animation Project Stop motion (also known as stop action) is an animation technique to make a physically manipulated object appear to move on its own. The object is moved in small increments between individually photographed frames, creating the illusion of movement when the series of frames is played as a continuous sequence (video). Objective Study the behavior of chromosomes during mitosis and see the phases of Mitosis and Meiosis in motion. 1.6.5 Describe the events that occur in the four phases of mitosis (prophase, metaphase, anaphase and telophase. Materials Candy, play dough/clay, a paper/poster to use as your background, pencils, markers, index cards to label your names and phases, a digital camera/phone Technology You will upload your pictures into a program that will help you create your animation. JellyCam (see web address below) is a free download that will upload your pics and create the animation for you. It does limit you in that you cannot add sound. iMovie or Windows Movie Maker is available also but it will be a little more labor intensive. Or you can use any app (“stop motion studio” is a good one , program or any other website you find that stitches your pictures together. OR, you can use any other program I listed below. Grading See rubric on last page Schedule Day 1 – Introduction (flip class), planning and drawing your phases Day 2 & 3 – Taking pictures Day 4- Uploading to computer and creating animation- YOU WILL DO THIS AT HOME Web Resources http://www.stopmotionpro.com/ - free trial http://microsoft-photo-story.en.softonic.com/ - free program 1 http://www.jellycam.co.uk/ - you want to check this OUT- it’s FREE!!! http://www.nchsoftware.com/videopad/index.html?gclid=COqru4uN4K4CF Q7sKgodfXrDUg also free, BUT ONLY through this link!!!! http://www.ikitmovie.com/free-trial-stop-motion-software.php - free trial or any other app, program or online service you can find that does “stop motion animation”. Procedure (READ ALL OF THEM BEFORE YOU START): 1. On an index card, post it note, or any piece of paper, write your names, and title of your animation. 2. The following phases and labels will be required on your pictures (as appropriate) Interphase Prophase Metaphase Anaphase Telophase Cytokinesis Centromere Sister Chromatids Nuclear membrane Cell membrane Chromosomes Centrioles Spindle fibers Cleavage furrow 3. Using available materials, construct a model of a cell starting with INTERPHASE. 4. LABEL ANYTHING in YOUR MODEL THAT WE NEED TO SEE or UNDERSTAND. DO THIS FOR ANY STAGE YOU WANT BY USING AN INDEX CARD, post it note or small pieces of paper. 5. Take a picture. 6. Make very small changes to your cell to transition from phase to phase. 7. Take a picture. 8. Try to take 5-6 pictures between each phase change while making small changes to your model to transform it into the next stage. 9. AT EACH PHASE, label the name of your phase under, next to or anywhere near your model. 10. The MORE pictures between stages as you move your cell parts, the better your video will look! 11. Take a minimum of 30 or more pictures of the different stages for Mitosis, Label the stages and important parts of the cell as you are taking your pictures. Upload into Stop-Motion software to create your Stop-Motion video. 12. Continue until you are done with all stages of Mitosis. 2 Procedure Questions Use these questions to help guide you as you create your film. Interphase: How does one chromatid compare to its sister chromatid? Can you tell the difference between the original and the replicated strand? What are visual clues that tell you that this cell is in Interphase? Prophase: What is the purpose of spindle fibers? How is this stage different from Interphase? Metaphase: At this point, the spindle fibers growing out of the poles resemble a starshaped structure. What is this structure called? Where do spindle fibers attach to the chromosomes? What can you use to remember what happens during this phase of mitosis? Anaphase: During this phase, how does the number of chromosomes change (when compared to prophase)? Telophase: Why are the chromosomes split and pulled to opposite poles? How does this help with cell division? What happens to the chromosomes at this point? What cellular parts disappear and what parts reappear at this stage of mitosis? Cytokinesis: What is the role of the cleavage furrow? How does this process differ for a plant cell? 3 Group Members Names: _______________________________________________________________ Planning Sheet You will need to plan what materials you will need to represent the cell parts and get your labels created so that when you are ready to take pictures, you can focus on just taking pictures rather than getting things together. Interphase: Important steps to highlight: What materials and labels do I need? Prophase: Important steps to highlight: What materials and labels do I need? Metaphase: Important steps to highlight: What materials and labels do I need? 4 Anaphase: Important steps to highlight: What materials and labels do I need? Telophase: Important steps to highlight: What materials and labels do I need? Cytokinesis: Important steps to highlight: What materials and labels do I need? 5 Tips for a better movie Use your stages of mitosis or meiosis that you drew in your EB worksheet. TRY and KEEP CAMERA and ZOOM in the same spot/position/angle as you take each picture. While one of you gets ready to take a picture, the other team member gets going in moving that parts of the cell. Be mindful of what else is in the frame to avoid things popping in and out of your video. Take more pictures than you need. Experiment if flash or no flash works better. Have fun and be creative. 6