Clone or not to clone

3/14/18 Notes
To clone or not to clone?
o Today
§ Review of SCNT
§ Scientific issues with cloning: How well does it work?
§ Societal issues: pros, cons, concerns, laws
§ Decision making!
o Sematic Cell Nuclear Transfer
§ Try to draw the process.
§ Process of cloning
• How dolly the sheep was cloned.
• Steps:
o 1. Remove the haploid nucleus from an egg (an enucleated
o 2. Remove a diploid nucleus from a somatic cell of the
organism to be cloned.
o 3. Insert the diploid nucleus into an enucleated egg
o 4. Stimulate the cloned zygote to divide with an electric
o 5. For reproductive cling insert the cloned embryo into a
surrogate mother
o 6. Surrogate is not necessary for therapeutic stem cells
o SNUPPY: Born April 2005
§ Seoul National University Puppy
§ Took nearly 2,000 eggs to make 1,000 embryos
§ Only one healthy puppy
o Does SCNT Produce a true clone?
§ Mitochondria: eukaryotes
§ Mitochondria
§ Animals produced by SCNT have the mitochondria of the egg donor (not
the DNA donor)
o Cloning Myths
§ Can clones reproduce?
• Generally, yes!
• The Gaur example was sterile.
§ Are the offspring of clones also clones, or weaker genetically?
• No!
o Problems associated with cloning.
§ Not an efficient process
§ Health of cloned animal
• May be overweight
• One in three are born abnormally large or with other
developmental problems
• Dolly developed arthritis at an early age
• Shortened telomeres
o Society and decision-making case
§ Handouts
3/14/18 Notes
Analyzing the CNN Clonaid press release
Post truth society: give an opinion without having proper evidence to back
it up and it is socially accepted. Recent in the last couple of years.
• Open discussion about Post truth.
Critical questions to ask about this article?