3/14/18 Notes • To clone or not to clone? o Today § Review of SCNT § Scientific issues with cloning: How well does it work? § Societal issues: pros, cons, concerns, laws § Decision making! o Sematic Cell Nuclear Transfer § Try to draw the process. § Process of cloning • How dolly the sheep was cloned. • Steps: o 1. Remove the haploid nucleus from an egg (an enucleated egg) o 2. Remove a diploid nucleus from a somatic cell of the organism to be cloned. o 3. Insert the diploid nucleus into an enucleated egg o 4. Stimulate the cloned zygote to divide with an electric current o 5. For reproductive cling insert the cloned embryo into a surrogate mother o 6. Surrogate is not necessary for therapeutic stem cells o SNUPPY: Born April 2005 § Seoul National University Puppy § Took nearly 2,000 eggs to make 1,000 embryos § Only one healthy puppy o Does SCNT Produce a true clone? § Mitochondria: eukaryotes § Mitochondria § Animals produced by SCNT have the mitochondria of the egg donor (not the DNA donor) o Cloning Myths § Can clones reproduce? • Generally, yes! • The Gaur example was sterile. § Are the offspring of clones also clones, or weaker genetically? • No! o Problems associated with cloning. § Not an efficient process § Health of cloned animal • May be overweight • One in three are born abnormally large or with other developmental problems • Dolly developed arthritis at an early age • Shortened telomeres o Society and decision-making case § Handouts 3/14/18 Notes § § § § Analyzing the CNN Clonaid press release Post truth society: give an opinion without having proper evidence to back it up and it is socially accepted. Recent in the last couple of years. • Open discussion about Post truth. Critical questions to ask about this article?