Ollscoil na hÉireann, Gaillimh National University of Ireland, Galway GX_____ Autumn Examinations 2004/2005 Exam Code(s) Exam(s) 2BS1 2nd Year Science Examination Module Code(s) Module(s) MI211 Microbiology – The Microbial Cell Paper No. Repeat Paper 1 Special Paper External Examiner(s) Internal Examiner(s) Professor C. M. Brown Professor J. A. Houghton Dr. C. O’Byrne Instructions: Answer 5 questions Please indicate clearly the numbers of the questions answered on the first page of your answer book Duration No. of Pages Department(s) Course Co-ordinator(s) Requirements: Handout MCQ Statistical Tables Graph Paper Log Graph Paper Other Material 3 Hours Cover page + 1 Microbiology Dr. Conor O’Byrne Q1. Write an essay on “The prokaryotic cell”. Q2. Discuss the methods available for the study of bacterial cytology. Q3. Write an essay on “Protein translation in bacteria”. Q4. Define the terms “horizontal DNA transfer” and “vertical DNA transfer”. Describe the types of horizontal DNA transfer that occur in bacteria. Q5. Outline the typical constituents of a bacterial growth medium and explain the reason for the inclusion of each. Q6. With the aid of diagrams explain in detail how you would perform a “viable cell count” on a liquid suspension of microorganisms. Q7. Describe in detail, the operation of the electron transport chain and associated oxidative phosphorylation in eukaryotes. Briefly comment on the differences between eukaryotic and prokaryotic processes. Q8. Write notes on two of the following: (a) (b) (c) Transport of sugars into bacterial cells Substrate level formation of high-energy compounds in microbial metabolism Enzyme-substrate interactions