OLLSCOIL NA hEIREANN, GAILLIMH NATIONAL UNIVERSITY OF IRELAND, GALWAY SEMESTER ONE EXAMINATION 2000/2001 SECOND SCIENCE MICROBIOLOGY EXTERN EXAMINER Professor C M Brown INTERN EXAMINER Internal Examiners TIME ALLOWED: 3 hours ANSWER FIVE QUESTIONS (INDICATE CLEARLY THE QUESTIONS ANSWERED ON THE FIRST PAGE OF YOUR ANSWER BOOK) 1. Write an essay titled "Gene transfer in bacteria". 2. Describe the process of transcription of messenger RNA (mRNA) in bacteria. 3. Compare and contrast the prokaryotic and eukaryotic cell. 4. Describe the structure and function of the bacterial cytoplasmic membrane. 5. Temperature has a fundamental influence on the growth of microorganisms. Discuss. 6. Compare and contrast batch culture and continuous culture. Include relevant diagrams when describing these techniques. 7. Compare and contrast homolactic and heterolactic fermentation pathways. 8. Describe in detail the operation of the electron transport chain and associated oxidative phosphorylation in eukaryotes. Briefly comment on the differences between eukaryotic and prokaryotic processes.