Figure 2. Differentiation of human ES cell-derived neuroepithelial cells in vivo. The neuroepithelial cells, transfected to express green fluorescent protein (GFP), were transplanted into newborn immunodeficient SCID mice through intraventricular injection. One month after implantation, many of the grafted GFP+ cells are positively labeled with βIII-tubulin (red in A) and fewer cells are labeled with MAP2 (red in B). Arrows indicate the GFP+ cells that colabel with βIII-tubulin in (A) or MAP2 (B). After 3 months, glial differentiation is observed as shown by the expression of GFAP (D) in some of the GFP-expressing cells (C). (E) is a merged image of (C) and (D).