1. Make a 3 ml LBamp (c f
= 100 µg/ml) overnight for each sample. Incubate a 37°C in water bath shaker for 16 hours.
2. Transfer 30 µl (1:100 dil) of each overnight to a tube containing 3 ml LBamp (c f
= 100 µg/ml) + IPTG (c f
= 1 mM). Incubate a
37°C in water bath shaker for 24 hours.
3. After 24 hours, transfer 500µl of each culture to a 1.7 ml centrifuge tube.
4. Centrifuge the tubes at 11000 rpm for 1 minute. Remove supernate.
5. Add 500µl of HN (GFP buffer) to the pellet.
6. Vortex to resuspend the cells.
7. Centrifuge the tubes at 11000 rpm for 1 minute. Remove supernate.
8. Repeat steps 5-7.
9. Add 500µl of HN (GFP buffer) and vortex to resuspend the cells.
10. Use 96 well clear bottom microtiter plate for the assay. Add 100
 l of each sample to the wells on the plate. Also, add 100
 l of HN (GFP buffer) to a well.
11. Warm-up the Spectramax 190 and Spectramax Gemini for 15 minutes.
12. Set up the plate templates.
13. Measure the absorbance (600 nm) and fluorescence(ex:395, em:509) three times. Use
automix.