Secreted Extracellular Vesicles
Types/Characteristics
Biogenesis
Lipid bilayer
Microvesicles
Secretory
pathway
Carrier
vesicle
Secretory
vesicle
Functions
Integrins
Microvesicles
Multivesicular bodies (MVB)
from other organelles
CD40
Size:
100-1000nm
Shape: Irregular
Markers: Intergins, selectins,
CD40 ligand
Lipids: Phosphatidylserine
Origin: Plasma membrane
Various secreted extracellular vesicles
are present in urine, amniotic fluid,
bronchoalveolar lavage fluid, breast milk,
saliva and blood.
Cellular uptake mechanisms
Selectins (CD62)
?
Exosome-like
vesicles
Golgi
TNFRI
Nucleus
Endoplasmic
reticulum
Early/sorting endosome
Fusion to
create late
endosomes
Exosomes
Tetraspanins
(CD63/CD9)
Alix
TSG101
ESCRT
mitochondrial DNA
mRNA
miRNA
Release
Lysosome
Degradation
Size:
20-50nm
Shape: Irregular
Markers: TNFRI
Lipids: No lipid rafts
Origin: MVB from other organelles?
Physiological functions
Receptors
Endocytosis
Multivesicular
endosomes
Exosome-like vesicles
• Ligand/receptor interactions
• Attachment and fusion via integrins
and cell adhesion molecules
• Transcytosis
Exosomes
Size:
50-100nm
Shape: Cup shaped
Markers: Tetraspanins (CD63/CD9),
Alix, TSG101, ESCRT
Lipids: Cholesterol, sphingomyelin,
ceramide, lipid rafts,
phosphatidylserine
Origin: Multivesicular endosomes
Membrane particles
CD133
Membrane
particles
Size:
50-80nm
Shape: Round
Markers: CD133, no CD63
Lipids: Unknown
Origin: Plasma membrane
• Inter-cellular communication
• Disposal of defective or effete proteins
• Formation of morphogen gradients
for tissue patterning during
development
• Antigen presentation to T cells
Pathological functions
• Transmission of viruses, prions and
b-amyloid in Alzheimer's disease
• Tumor pathogenesis
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Produced in collaboration with Claudie Hooper, Institute of Psychiatry, King’s College London, UK
Copyright © 2011 Abcam, All Rights Reserved.
Cells use vesicular and tubular transport to carry cargo between destinations in the cytoplasm as well as secrete proteins such as hormones
and neurotransmitters into the extracellular space. Cells also exhibit intercellular communication using secreted vesicles such as exosomes,
microvesicles and membrane particles. These archetypes represent a spectrum of overlapping classes of vesicles. Microvesicles and
membrane particles originate from the direct budding of the plasma membrane whereas exosomes form within endosomes (sorting
endosome) giving rise to multivesicular endosomes. They then fuse with the plasma membrane generating exosomes or are directed to
lysosomes for degradation. Similar secreted vesicles might form within other organelles generating exosome-like vesicles.
Secreted Extracellular Vesicles
Related antibodies from Abcam
Product
Host
Species
Reactivity
Product
code
Rb
Rb
Rat
Hu, Ms, Rat
Hu, Ms, Rat, Dog, Pig
Ms
ab2899
ab18199
ab8158
Rb
Hu, Ms, Rat, Cow, Gpig,
Hm, Mk, Pig, Rb, Shp
Hu
Hu, Ms, Mk
Hu, Ms, Rat, Hm
Hu, Ms
Hu, Ms, Rat, Cat, Chk,
Ql, RMk
Hu
Hu
Hu, Ms, Rat
Dog, Hm, Hu, Ms, Rat, Zfsh
Hu
Hu, NHuPrm
Hu, Ms, Rat, ChHm, Dog
Hu, Ms, Rat, Cow, Dog, Hm
Ms, Rat, Cow
Hu, Ms, Rat
Hu, Ms, Rat, Cow, Dog, Dm,
Hm, Mk, Pig, Rb, Shp, Xl
Hu, Rat
Hu, Ms, Rat
Hu, Ms, Rat
Ms, Rat
ab13545
Featured antibodies
Antibodies
beta COP
Caveolin 1 - Caveolae Marker
CD34 [MEC 14.7] Hematopoietic Stem Cell Marker
CD40
P
P
M
P
ICC/IF, IP, WB
ICC/IF, IHC-P, WB
ELISA, Flow Cyt, ICC/IF, IF, IHC-FoFr,
IHC-Fr, IHC-P, IP, WB
WB
CD82 [B-L2] - Azide free
Chromogranin A
Clathrin heavy chain
DCTN1
Doublecortin - Neuronal Marker
M
P
P
P
P
Flow Cyt, ICC/IF, IHC-Fr, IHC-P
ICC/IF, IHC-Fr, IHC-P, WB
Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IP, WB
ICC, ICC/IF, IHC-Fr, IHC-P, WB
ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, WB
Ms
Rb
Rb
Goat
Rb
Integrin alpha 2+beta 1 [16B4]
Integrin beta 1 [12G10]
Integrin linked ILK
LAMP1
PDC6I
Rab11
Rab5 - Early Endosome Marker
Rab9 [Mab9]
SNAP25
SQSTM1 / p62
TNF Receptor I
M
M
P
P
P
P
P
M
P
M
P
ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
ELISA, Flow Cyt, ICC/IF, WB
ICC/IF, IHC-P, WB
ICC, ICC/IF, IHC-P, IP, WB
ICC/IF, WB
ICC/IF, WB
ICC/IF, IHC-Fr, IHC-FrFl, IP, WB
Flow Cyt, IHC-P, WB
ICC, IP, WB
ICC/IF, IHC-P, WB
Flow Cyt, ICC/IF, IHC-P, WB
Ms
Ms
Rb
Rb
Ms
Rb
Rb
Ms
Rb
Ms
Rb
TOMM20
TSG101
Twist [Twist2C1a] - ChIP Grade
VAMP2
M
P
M
P
Flow Cyt, ICC/IF, IHC-P, WB
ICC/IF, WB
ChIP, Dot, ICC, ICC/IF, IHC-Fr, IHC-P, WB
ICC/IF, IHC-Fr, IP, WB
Ms
Rb
Ms
Rb
Target
Species Sample type
Assay
Type
Sensitivity
ab47153
ab15160
ab21679
ab1180
ab18723
ab30483
ab30394
ab228
ab24170
ab88743
ab3612
ab18211
ab2810
ab5666
ab56416
ab19139
Hu
Hu
Hu
Hu
Cell culture supernatant, urine, serum, plasma
Cell culture supernatant, urine, serum, plasma
Cell culture supernatant, urine, serum, plasma
Cell culture supernatant, serum, plasma
Discover more at abcam.com
Sandwich
Sandwich
Sandwich
Sandwich
< 50 pg/ml
< 6 pg/ml
< 30 pg/ml
< 1 ng/ml
Clonality Applications
M
ICC/IF, IHC-Fr, IHC-P, IP, WB
Host
Ms
Species cross reactivity
Cow, D dis, Dog, Hu, Ms,
Phy p, Pig, Rb, Rat
Host
Ms
Species cross reactivity
Hu, RMk
Host
Rb
Species cross reactivity
Hu
CD62P [AK-6] (ab6632)
Clonality Applications
M
Flow Cyt, IHC-Fr, IHC-P, IP, WB
PDC6I (ab76608)
Clonality Applications
P
ICC/IF, WB
ab56783
ab30871
ab50887
ab3347
Product
code
ELISA Kits
CD40
CD40L
CD62E
CD62L
Actin [ACTN05 (C4)] (ab3280)
ab99990
ab99991
ab100512
ab45917
Tubulin [YOL1/34] - Microtubule Marker (ab6161)
Clonality Applications
M
ELISA, ICC/IF, IHC-FoFr,
RIA, WB
Host
Rt
Species cross reactivity
Hu, Ms, Rat, Allg, Dog,
Sc, S pom
139_11_AKR
­
Clonality Applications