CENTENNIAL HONORS COLLEGE
Western Illinois University
Undergraduate Research Day 2015
Poster Presentation
Analysis of Fenuron by High Performance Liquid Chromotography
Rachel Austin
Faculty Mentor: Brian Bellott
Forensic Chemistry
High performance liquid chromatography (HPLC) uses analytical techniques like reverse phase separation and
internal standards. Separation of analytes allows for specific detection and qualitative analysis. Use of an internal
standard helps with quantitation. Fenuron is a phenyl-urea herbicide normally used to treat perennial weeds and
unwanted woody plants. Another new use is as an aquatic herbicide because of its low toxicity to fish. A sample of
the herbicide will be obtained and diluted into six different concentrations using methanol. These different samples
will then be spiked with an internal standard. Each sample will be analyzed via high performance liquid
chromatography (HPLC) three times in order to determine the baseline reading for each concentration.