CENTENNIAL HONORS COLLEGE Western Illinois University Undergraduate Research Day 2015 Poster Presentation Analysis of Fenuron by High Performance Liquid Chromotography Rachel Austin Faculty Mentor: Brian Bellott Forensic Chemistry High performance liquid chromatography (HPLC) uses analytical techniques like reverse phase separation and internal standards. Separation of analytes allows for specific detection and qualitative analysis. Use of an internal standard helps with quantitation. Fenuron is a phenyl-urea herbicide normally used to treat perennial weeds and unwanted woody plants. Another new use is as an aquatic herbicide because of its low toxicity to fish. A sample of the herbicide will be obtained and diluted into six different concentrations using methanol. These different samples will then be spiked with an internal standard. Each sample will be analyzed via high performance liquid chromatography (HPLC) three times in order to determine the baseline reading for each concentration.