SEED QUALITY
OF MAIZE INBRED LINES WITH
DIFFERENT OIL AND
PROTEIN CONTENTS AND
GENETIC BACKGROUND.
Miriam Munamava, Susana Goggi and Linda Pollak
Iowa State University and USDA-ARS

 Corn breeding program: crossing and selecting for oil and protein.
 Stiff Stalk crossed with germplasm from Argentina.
 Non- Stiff Stalk crossed with germplasm from St. Croix.
 Developed lines of different oil and protein content.

 To characterize the inbred lines for seed quality.
 To evaluate the effect of oil and protein levels on seed quality.
 To evaluate the effect of genetic background and production location on seed quality.

 Seed production
Ames-IA
Clinton-IL
Colombia-MO
Hooper-NE
Starkville-MS

Entry
7
8
9
10
3
4
1
2
5
6
Table 1 Description of Germplasm used in the Study
Seed composition
High protein
High protein
High protein
High protein
High oil
High oil
High oil
Low oil
Genotype
SC01:N1310-378-11-1-1-7-3-B
SC01:N1310-378-11-1-1-4-4-B
AR16035:S02-325-1-1-6-4-7-B
AR16035:S02-325-1-1-6-6-11-B
SC01:N1310-338-31-6-2-1-6-B
AR16035:S02-325-1-1-6-6-3-B
AR16035:S02-325-1-1-6-3-13-B
AR16035:S02-325-1-1-6-3-9-B
B73
MO17

Planting on Paper towels Seedling evaluations

Saturated cold test Seeds planting

 Leakage of electrolytes: amino acids and organic acids.
 Poor membrane integrity in deteriorated, low vigor seeds.
e e e e -

 Imbibe seeds in deionized water for 24 hs at 25 o
C
 Individual Seed
Analyzer

 Increased temperature
Temperature: 42 o C for 96h
 High relative humidity
 Planting:
Over CCP covered with sand

 Soak test
 Fast green (FG)
 100 seed weight
 Inbred quality index (IQI)
IQI =
SC+AA+FG+Soak + [ 200- (EC6h+EC24h)]
60

Results and Discussion

Table 2. Levels of significance from ANOVA for ten maize inbred lines evaluated in four locations.
Variable
Standard germination
Cold test
AA
EC
Soak test
Seed weight
Inbred
***
**
**
***
***
***
Inbred*location
***
***
***
***
***
***

Table 3. Seed vigor tests comparisons between groups of lines bulked by exotic genetic background, Argentina (AR) and St. Croix
(SC), and seed composition, high oil (HO), low oil (LO).
Contrast
AR vs. SC
Cold ns
HO vs. LO *
B73 vs. AR ns
MO17 vs. SC ns
AA ns ns ns ns
EC
* ns ns
*
Soak
*
** ns
**

Table 4. Means cold test results
IA MO
Locations
IL
1
2
3
4
5
6
7
8
HP
HP
HP
HP
HO
HO
HO
LO
9 B73 71
10 MO17 57
LSD (0.05) 32
57
88
75
81
60
52
69
77
37
43
51
59
37
73
55
53
57
65
22
65
39
77
60
73
84
52
52
77
40
23
NE
36
77
71
80
61
80
80
68
59
48
24

Table 5. Means AA test results.
1
2
3
4
5
6
7
8
9
LSD
HP
HP
HP
HP
HO
HO
HO
LO
B73
10 MO17
(0.05)
IA
37
41
48
65
43
57
43
45
45
56
32
Locations
MO IL
..………………..%....………………
41
63
47
59
68
81
22
51
75
64
63
77
63
87
67
53
89
24
7 7
67
93
56
NE
35
76
84
53
64
61
26
51
60
48
88

1 HP
2 HP
3 HP
4 HP
5 HO
6 HO
7 HO
8 LO
9 B73
10 MO17
LSD (0.05)
Table 6 . Means electrical conductivity results (24h).
Locations
IA MO IL
..…………………… m s ..…………………
NE
5.0
7.0
4.5
7.3
3.5
4.6
4.6
4.5
3.0
7.1
4.9
4.3
3.6
5.3
4.5
5.0
3.9
3.7
6.5
2.1
4.2
3.4
9.5
4.1
4.8
3.7
4.0
3.7
4.5
4.8
3.9
3.7
5.2
2.0
2.6
4.2
2.3
3.0
2.4
4.2
1.5
2.1
2.0
2.3

Table 7 . IQI for different locations.
IA MO
Locations
IL
4
5
6
1
2
3
7
8
9
10
LSD (0.05)
5.7
4.2
6.2
8.1
4.1
7.1
5.7
6.0
7.0
6.6
1.5
4.4
7.1
5.2
5.0
5.4
6.5
5.4
4.4
7.9
8.4
1.8
4.8
7.9
6. 9
8.1
8.1
2.1
7.1
7.2
8.0
4.5
8.9
NE
5.0
6.4
6.3
7.5
1.6
6.7
7.3
6.2
8.0
6.0
2.6

Table 8. IQI and relative ranking of the inbred lines.
1 HP
2 HP
3 HP
4 HP
5 HO
6 HO
7 HO
8 LO
9 B73
10 MO17
IQI
5.0
6.3
6.6
5.9
7.7
5.4
6.3
6.5
6.3
7.2
Ranking
8
1
2
10
5
4
3
5
9
5

 Low protein lines have the lowest seed vigor.
When protein levels were high, seed quality was high regardless of oil content.
 Mo17 and B73 have the highest IQI. Inbred
Quality Index results suggests that seed quality attributes should be considered in a comprehensive breeding program.
 Inbred line 4 (high protein) has the highest vigor when produced in IA and NE.

 Inbred lines with Argentina germplasm have the highest seed quality.
 Seeds produced in IL have the best quality, while those produced in MO have the lowest quality.

 Tom Hoegemeyer- NE
 Kevin Montgomery- IL
 David Willmot - MO
 Paul Williams- MS
 Penny Meyerholz
 Phillip Dixon (ISU Stats)