LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – APRIL 2012
PB 5408 - BIOPHYSICS
Date : 30-04-2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART A
Answer the following, each answer within 50 words:
(10 x 2 = 20 marks)
1. Define Brownian movement.
2. What is sedimentation?
3. Define osmosis.
4. What is a buffer?
5. Mention any two properties of colloids.
6. What is isoelectric focusing?
7. Define Beer-Lamberts law.
8. What is fluorescence?
9. Define radioactivity.
10. What is free energy?
PART B
Answer the following, each answer within 500 words:
Draw diagrams wherever necessary:
(5 x 7= 35 marks)
11. a. Write notes on sedimentation and factors influencing it.
(or)
b. Give a brief account of the kinetic theory of gases.
12. a. Explain in brief about osmosis and factors affecting osmosis.
(or)
b. Write notes on the various methods for preparing solutions of different concentration.
13. a. Give an account of colloids, their properties and classifications.
(or)
b. Write short notes on the principles of chromatography.
14. a. Explain briefly about the properties of light.
(or)
b. Discuss in brief about the fluorescence microscopy and its applications.
.
15. a. Write short notes on radioactive decay.
(or)
b. What are the various laws of thermodynamics?
PART C
Answer any three of the following, each answer within 1200 words:
Draw diagrams wherever necessary:
(3 x 15= 45 marks)
16. Discuss in detail about the principles and types of centrifugation.
17. What is pH? Write detailed notes on the measurement of pH and the biological importance of pH.
18. Explain in detail about various types of chromatography.
19. Give a detailed explanation on the structure and functioning of scanning electron microscopes.
20. What are radioisotopes? Explain in detail about the applications of radioisotopes in biology.
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