Staining
of Bacteria
Hana ŠURANSKÁ
Brno University of Technology, Faculty of Chemistry
Staining
A biochemical technique of adding a
class-specific dye
Stains and dye – to highlight cellular
structures
DNA, proteins, lipids, carbohydrates
Different stains react in different
parts of a cell or tissue
Microscopes
Dye
A colored substance
Used to highlight
cellular structures
Applied in aqueous
solutions
Carmine, crystal
violet, DAPI, eosin,
ethidium bromide..
Eosin
A red fluorescent dye
Stains cytoplasm, collagen and muscle
fibers
Most often used as a counterstain
An acidic dye
It shows up in the basic parts of the
cell (nucleus)
DAPI
4,6-diamidino-2-phenylindole
A fluorescent stain
It binds strongly to DNA and RNA
Used in fluorescence microscopy
Excited with ultraviolet light
Methods of staining
Gram staining
Gimenez staining
Giemsa staining
Ziehl-Neelsen staining
Eosin staining
Gram staining
Hans Christian Gram (1853–1938)
An empirical method of differentiating
bacterial
Gram-positive, Gram-negative
in 1884
It uses crystal violet, iodine, fuchsine,
safranin
Giemsa staining
Gustav Giemsa
It can be used to study the adherence of
pathogenic bacteria to human cells
A mixture of methylene blue and eosin
Visualize chromosomes
Ziehl-Neelsen
staining
Mycobacterium tuberculosis
The acid-fast stain
Franz Ziehl and Friedrich Neelsen
A special bacteriological stain
It is used to identify acid-fast
Mycobacteria
Micture of carbolfuchsine and
methylene blue
Thank you
for your
attention!