Cells in Mitosis Mitosis in Onion Cells! • You will be examining onion root tip cells. These cells are used because the root tip is growing. – In order for tissue to grow, cells must either: – 1) get larger, or – 2) divide to form new cells (cell cycle and mitosis) • These root tips have hundreds of cells growing and dividing (undergoing mitosis). – Note: if mitosis goes badly (chromosomes don’t line up or separate appropriately), the new cells probably won’t survive. Onion root tip cells under high power • • A few cells dividing Lots of cell dividing Your field of view should look similar to these! If they don’t, look at another part of the root tip. Interphase • C= Chromatin (“spread out DNA” – chromosomes not visible yet) I= Nucleolus Prophase • Chromosomes visible, but unorganized • A “pile” of visible chromosomes Metaphase • Chromosomes lined up in middle of cell Anaphase • Chromatids begin to pull apart Telophase in onion cells Chromatids reach opposite ends of the cell Why is the cell not pinched in the middle? Telophase in food (ha!) Instructions: • • • • Choose who will read directions (you or your partner). No sign in. Onion slides are either with me or on your microscope stage. No books! Just notes. Part I: Draw 5 cells in different phases of mitosis and describe them! – Note: one entire cell should fill a box on your handout. – DRAW WHAT YOU SEE, not what you THINK you should see. – If you are not sure which phase a cell is, give your best guess – or ask Mr. Welman for help! You may use your Cornell notes, too – You will NOT see spindles/centrioles. Don’t draw them. – I will only stamp part II if your drawings are accurate and awesome in part I. Take your time. You have tomorrow, too. – It can be challenging to find some phases (e.g. metaphase). Be patient, keep looking. – In Part I you must show me one phase (not Interphase) Day 2 • Part I: Finish what you started yesterday – find and draw 5 phases – Get a stamp after Part 2 • Show me one identified cell – NOT interphase – from Part 1 I will only stamp if drawings are accurate and excellent. Take your time. • Part II: Scan 25 additional cells, determine what phase each one is in, and record this data. Get a stamp after you have completed this. 4 interphase 2 prophase 1 anaphase (do this for 25 cells…) If you finish early, work on homework. Books available on the cart. Your answers must clearly be your own.