Do Now 1… page 1. How is a plant cell different from an animal cell? 2. What life functions do the following cell organelles carry out? a) Mitochondria b) Cell membrane c) Endoplasmic Reticulum d) Ribosomes 3. Where are enzymes synthesized? 4. What makes one protein different from another? Complete the Cell Membrane Reading Do Now 2… page Compound Starch Lipid Enzyme Big Molecule Monosaccharide Amino Acid Small Molecule Polysaccharide Amino Acid Glucose Protein Fatty Acid Iodine Atom Cell Membrane How are things moved within a cell? How does a cell get rid of waste and take in nutrients? Hmm… If a cell needs glucose to make energy, what does the glucose have to pass in order to get into the cell and to the mitochondria? Cell Membrane Also known as: Plasma Membrane Cell Membrane: Function & Purpose Cell Membrane Functions: Regulates what enters and leaves the cell (ingestion & excretion) Also allows cell communication, identification, and allows the ingestion and excretion of certain materials Life Functions = Regulation , Excretion and Transport Homeostasis Helps the cell maintain __________________ by regulating what enters and leaves the cell. Cell Membrane only lets CERTAIN things in and out! Semi-permeable because it only lets The cell membrane is _________________ certain substances in and out. *Regulation Cell Membrane: Structure What are the parts that make up the Cell Membrane? Cell or Plasma Membrane is sometimes referred to as a Phospholipid Bilayer Why? Lets take a look… Made of two layers of… Phospholipids Phospholipid: Polar head containing the element phosphorous Attracted to water (hydrophilic) Non-polar fatty acid tail Repels/hates water (hydrophobic) Phospholipid Bilayer Why is the membrane a bilayer? • Makes it hard for the membrane to open up / break Cell Membrane has more than just phospholipids… The cell membrane also has many proteins inside it! Help in cell communication and transport Types of Membrane Proteins 1. Channel proteins 2. Receptors 3. Glycoproteins Membrane Proteins 1. Channel Proteins (Transport Proteins): Molecules that are a little to large to pass into or out of the cell use channel proteins to enter or leave. 2. Receptor Proteins: Transmit information into the cell by reacting with certain molecules and chemicals. The part that sticks out has a specific shape and only molecules with the matching shape can bind to it. • This is how some pathogens attach to and enter cells! 3. Marker Proteins (Glycoproteins): The name tags of the cell. Gives each cell its own identity. Label Your Diagrams! Cell Membrane: Fluid Mosaic Model The cell membrane is a thin flexible boundary between the cell’s internal environment and the outside environment Fluid – Phospholipids and associated proteins move around like water in the ocean. The membrane is flexible. (like a bubble) Mosaic – So many different pieces make the membrane look like a mosaic or quilt. Do Now 3… page Name the molecule or structure: 1. Communicates messages to a cell. Found in the cell membrane. 2. Only 1 atom big and is able to pass through a semipermeable membrane. 3. Breaks down and synthesizes molecules needed by a cell. 4. Regulates the movement of molecules in and out of a cell 5. This structure allows slightly larger molecules to pass through the cell membrane. Cell Membrane: Transport & Movement Cell Membrane: Transport & Movement What is transport? The movement of material from one place to another There are 2 ways that material moves into and out of a cell: 1. Transport that requires energy (ATP) is known as: Active Transport 2. Transport that does not require energy is known as: Passive Transport or Diffusion Selectively Permeability of the Cell Membrane Cell membrane DOES NOT let everything in and out Some materials cross membranes easily: Small molecules & Compounds water, oxygen, 1. ________________________________: CO2, amino acids, glucose Some materials do not cross membranes easily: Large Compounds Starches, proteins and other 1. ___________________: macromolecules Hmm… Generally, what tells if a molecule will easily pass through a membrane? The SIZE of a compound! But why do molecules move? Homeostasis 1. The cell wants to maintain __________ – Certain compounds enter the cell in order to maintain a stable internal environment. For example: if a cell is low on oxygen, oxygen will move into the cell. 2. Concentration Gradient – Difference in concentration where High concentration to areas of molecules move from areas of _______ Equilibrium Low ________ concentration to maintain a dynamic_______________. High Low Dynamic Equilibrium = Homeostasis During dynamic equilibrium, molecules are still moving back and forth across the membrane but at an equal rate. Small molecules pass through the cell membrane directly by squeezing through the phospholipids. Slightly larger molecules cannot do this so they must use __________________ Channel Proteins DO NOW Draw arrows and show movement of molecules **Will the molecules even pass the semipermeable membrane? Why? Two types of Transport 1 Passive Transport: No energy required 1. Diffusion 2. Facilitated Diffusion 3. Osmosis 2 Active Transport: Uses energy (ATP) for transport 1. Endocytosis 2. Exocytosis 3 Types of Passive Transport 1. Diffusion Diffusion – movement of materials from an area of High concentration (more molecules in one area) to an area of Low concentration (fewer molecules in an area) Diffusion is used when materials are small enough to easily diffuse across the membrane. Diffusion: Before & After 2. Facilitated Diffusion Facilitated diffusion – molecules move from areas of high to low concentration using a carrier or transport protein. big Some molecules, such as glucose, are a little too __________to pass through the membrane by simple diffusion Transport proteins These molecules need help and use ______________________ to pass through the membrane 3. Osmosis water from an area of high Osmosis – Diffusion of _________ concentration to an area of low concentration. 3. Osmosis Water will always move to where there is more dissolved substance (solute) in water. Or, water will move from where it is more concentrated to where it is less concentrated. To maintain equilibrium http://www.stolaf.edu/people/giannini/flashanimat/transport/osmosis.swf 3. Osmosis Practice questions: 1. Cell contains 10% salt and outside the cell contains 20% salt. Where will water come from and go to? 2. Cell contains 10% salt and the cell is put in distilled water (100% water). Where will water come from and go to? 3. Osmosis Practice question: 3. Kenny was on a boat and forgot to pack water. He made a bad move and drank salt water from the ocean. What will happen to the cells in his body? Practice Problems 4. Why do restaurants soak their lettuce and vegetables in water before they serve them?! Which cells are at equilibrium, in slat water and in distilled water? A= B= C= Do Now # Label the cell wall and cell membrane on the plant cell in the isotonic solution. Second, draw the plant cell when it is placed in a salt water solution (hypertonic solution) 2 Types of Active Transport Active Transport What is used in active transport that is not used in passive transport (diffusion)? Why would you need to use energy to transport molecules across a membrane? Active Transport • Movement of a substance across a membrane using ATP _____. • This occurs when molecules are moving from areas of LOW HIGH ____________ concentration to areas of __________ concentration. • Active transport occurs through special transport proteins that push/pull molecules into or out of the cell using energy Balloon! Active Transport Where is the concentration highest? How would blue diamond get into the cell? 1. Exocytosis Exocytosis – type of active transport when large material or large amounts of material leave the cell. Uses ATP Example – insulin leaves pancreatic cells by exocytosis Vacuoles inside cell fuse / connect will cell membrane and empty contents outside the cell. Exocytosis 2. Endocytosis Endocytosis – also known as phagocytosis, this type of active transport is when large macromolecules or large amounts of material enter the cell. • Cell membrane surrounds the macromolecule and creates a membrane around it. Practice Problems What happened to these plant cells?! Diffusion Lab Do Now Predict what will happen in this picture after diffusion occurs. Focus on the amount of molecules inside and outside the membrane. I – Iodine G – Glucose S – Starch What is this lab about? By the end of this activity you should be able to: • Demonstrate how to test for simple sugars and starch using chemical indicators • Explain diffusion through a membrane • Describe the permeability of a model membrane for glucose, starch, starch indicator solution (iodine) How do we make an artificial cell? Chemical Testing Indicator Solution Used Material Used Material Used Material Used _________________ _________________ _________________ Distilled Water Blue-Colored Glucose Indicator Solution Amber colored Starch Indicator Solution Starch Glucose What Actually Happened? In Cell? Outside of Cell? Before In Cell? Outside of Cell? After Stuff You Have to Know What are the building blocks of starch? What is Iodine? What is a chemical Indicator? Lugol’s Iodine indicates the presence of _____________ Benedict’s Solution indicates the presence of ___________ Do Now (Lab Part II) Why would a cell shrivel-up? What part of the cell would shrivel up? Explain Lab… Groups (1) 1. Group 1 – Adriel, Emma, Roberto, Katherine 2. Group 2 – Sebastian, Andreas, Kevin, Caleb, Jose 3. Group 3 – Kaylin, Diamond, Cesar, Wilkin 4. Group 4 – Jordan, Marcus, Jhadith, Eli 5. Group 5 – Danae’, Kevon, Johnelle, Sharel, Matt These are your groups… NO EXCEPTIONS Lab… Groups (5) 1. Group 1 – Gabby, Mia, Trayyonna, Jeffrey, Katty, Angel 2. Group 2 – Fallon, Jayln, Brandi, Brendon L, Jendy, Deandre 3. Group 3 – Eddison, Greg, Freddy, Anthony, Brandon G 4. Group 4 – Jada, Trevon, Chelsea J, Laura N, Chelsea R, Nagelis 5. Group 5 – Nyquan, Krystazia, Jakeim, Chris R, Nicole R, Steph These are your groups… NO EXCEPTIONS Lab… Groups (8) 1. Group 1 – Denise, Janiya, Jared, Jordan, Niamabi, Kyara 2. Group 2 – Nelgy, Luisa, Jeraldine, Nicole, Pam 3. Group 3 – Lisbeth, Silver, Kris, James, Gianny, Osiris 4. Group 4 – Karla, Ninoska, Keyli, Evelyn, Daniel, Hellin These are your groups… NO EXCEPTIONS What to expect Onion cells in distilled water What to expect Onion cells when salt water is introduced What to expect Onion cells in distilled water again Lab DO NOW Write answers on Loose leaf 1. If an artificial cell with a semipermeable membrane contained glucose and starch and was placed in water containing Iodine, what would happen? Why? 2. How can you tell if glucose is present in the water outside the cell? 3. How can you tell if starch is present in or outside the cell? Lab Do Now What happened to these cells? What might of caused this? Lab Do Now What are the building blocks of starch? What is Iodine? What is a chemical Indicator? Lugol’s Iodine indicates the presence of _____________ Benedict’s Solution indicates the presence of ___________