Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
Course Title and Number: Biology 2164 – Microbiology
Day(s) and Time(s):
Lecture- MC G27 5:30-6:50 MW
Lab – MC 629 7:00-9:50 pm M
Instructor:
Prof Judyth Gulden
Office hours: by appointment
TO CONTACT YOUR INSTRUCTOR
Phone: 918 289 0744
Email: judyth.gulden@tulsacc.edu
PREREQUISITES FOR THIS COURSE
BIO 1224 and CHEM 1215 or CHEM 1114
TO CONTACT THE DIVISION OFFICE
Division Name: Metro Science & Mathematics
Division Associate Dean: Dr. Connie Hebert
Office: MC 620
Phone: 918 595 7334
COURSE DESCRIPTION
General microbiology with emphasis on fundamentals of morphology, physiology, and growth related activities of
pathogenic and nonpathogenic disease producing organisms. Principles involved in susceptibility and resistance to
infections and the uses of immune sera and vaccines. Lecture: 3 hours. Laboratory: 3 hours.
COURSE PURPOSE
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Learning Objectives
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II.
Describe the structure, reproduction, metabolism and disease processes of the major groups of
bacteria and viruses.
Describe the major groups of bacteria from their microscopic, cultural, and diagnostic standpoints.
Explain host-microbe interactions including normal flora, transients and pathogens.
Outline the nonspecific and specific host defense mechanisms and the roles of various cells and
chemicals involved.
Describe the nutritional requirements of microbes and the consequent relationships to the disease
process.
Describe the diseases, transmissions, epidemiology, culturing and biochemical characteristics of select
genera and species of bacteria.
Perform the laboratory skills of general microbiology, including microscopy, staining, aseptic technique,
transfer, culturing and the use of diagnostic media.
Relate current trends in microbiology, from analysis of current research in recombinant DNA to gene
therapy, DNA sequencing, and monoclonal antibodies.
Demonstrate aseptic technique as it relates to personal life, laboratory procedures, and the medical
setting.
Explain microbial growth processes, their requirements for growth, the mathematics of microbial growth,
and the control of microbial growth in the laboratory, medical, and personal setting.
Student Learning Outcomes
The following are specific learning outcomes for the student taking this class:
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
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Describe and practice laboratory safety guidelines relating to working with chemicals, microorganisms,
body fluids and/or dissection.
Define and correctly use scientific terminology in regard to microbiological organisms and processes.
Synthesize information, think critically and solve critical thinking problems; write clear, well organized
answers to assignments that demonstrate synthesis.
Work well independently and show self-direction and motivation
Understand and follow directions properly
Show proficiency in taking exams, by responding to questions quickly and accurately, effectively
handling the pressure of a timed exam.
Assess personal needs in regard to study time and methods, and accept personal responsibility for the
learning process.
Apply principles of scientific inquiry, differentiate a theory from a hypothesis, and differentiate fact from
opinion in regard to microbiological sciences.
Assess and describe aspects of sustainability, including economic, societal and environmental factors,
especially in relation to microbiological systems.
TEXTBOOKS AND MATERIALS
All materials can be purchased at the bookstore.
Required Books and Materials
Microbiology with Diseases by Body System 4e – Bauman, Benjamin Cummings
Microbiology Laboratory Theory and Applications – Leboffe & Pierce, 9780895829672 (Custom Edition)
– Please note that if you buy other editions you will be responsible for getting the questions and
other materials found in the custom edition.
Students must have safety glasses that wrap around the eye and a lab coats for all labs. Students who do
wear goggles and/or lab coats during labs will not be allowed to participate in the lab. Student must have a
box of microscope slides, a lab notebook, and a black Sharpie permanent marker.
Safety policy: Students must wear safety glasses and protective gloves during labs. Students not wearing
proper safety equipment will not be allowed to participate in the designated laboratory exercise. Students
who consistently engage in unsafe practices in the lab may be asked to leave by the Instructor.
INSTRUCTOR SUPPORT
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Prof Gulden is available by phone, text, email or you can schedule an appointment. You are encouraged to call
the Instructor to discuss the material or any other items concerning the course.
Email will be checked daily, Monday through Friday from 9 am to 6 pm CST.
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
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Please note that if you send an email after those hours, it may not be addressed until the following business day
(Monday-Friday). This includes requests concerning exams or online assignments. When you email please put
your name, course and section number in the subject line of the email. This helps the instructor identify emails
that need immediate attention. For example:
Guldenbio1224section21032098
ATTENDANCE POLICY AND LATE ARRIVALS
The likelihood of succeeding in this course without your attendance is minimal. If you exceed more than 2 unplanned
absences from classes without contacting the instructor prior to the scheduled class you may be dropped from the
class at the instructor’s discretion. For late arrivals see Inappropriate Behavior in the Classroom below.
TEACHING METHODS
Lecture, videos, interactive and lab exercises, seminars, research activities, fieldtrips, and occasional guest
speakers. The course material will be divided into units representing closely related topics. Lecture will introduce the
major concepts. Each student is responsible for learning all assigned materials regardless of whether they
are presented through lecture or laboratory exercises.
Once each week students will participate in a Hands-On experience in the laboratory. Prior to any class meeting, it is
each student's responsibility to have read the appropriate assigned material.
EVALUATION TECHNIQUES
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Letter grades will be determined on the basis of the following:
Grading Scale
90-100%
80-89%
A
B
70-79%
60-69%
C
D
Assignment Categories
Exams [4@100 pts each]
Lecture Final Exam [100 - comprehensive] &
Lab Final Exam [60 comprehensive]
Unknown Lab Report [100pts]
Lab Assignments [13@15 pts]
Lecture Quizzes [4@10]
Lab Quizzes [3 @ 10]
Infectious Disease Paper [50] &
Presentation[25]
Total Points
400 pts
160 pts
100 pts
195 pts
40 pts
30 pts
75 pts
1000 pts
Exams and assignments will not be returned to students. A student may make an appointment to look over
their exams/assignment at any time before finals weeks. You may also make copies of your assignments
prior to turning them in.
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
Make up policy:
 Lecture exams and quizzes may not be made up.
 In the event that an exam or quiz is missed, the score on the next exam or quiz will be doubled and substituted
for the missed exam or quiz.
 Only one exam or quiz (excluding the final exam which must be taken) may be made up during the semester.
The student must inform the instructor BEFORE the date of the exam if they are going to miss it. This may be
done by email or phone. If this is not done prior to the date of the exam or quiz then the student will receive a
zero and may not take advantage of the makeup policy.
Assignment Presentation:
 All assignments are to be typed or in a format specified by the instructor.
 There are computers with word processing software available to any TCC student with a current ID. These are
located in the General User Microcomputer Lab on all campuses.
 All graphs must be generated using graphing software (e.g. excel).
 All work must be in a format that is supported by Microsoft Office unless directed otherwise by the instructor.
 The IT helpdesk and Computer Lab Services are available to provide support to students and help them with
technology related questions.
Late Laboratory and Short Assignments:
 Due dates are provided for all assignments.
 Late assignments will only be accepted up until the following class period. At that time, the assignment will lose
50% of the score.
 You may turn in assignments early.
OTHER POLICIES AND REQUIREMENTS
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Announcements pertaining to course activities will be made at the beginning of lecture and posted on
Blackboard. The student is responsible for obtaining all information made available during the
meeting periods from other students if they do not attend. All handouts, when possible, will be posted
on Blackboard. It is your responsibility to acquire them. Laboratory exercises, class presentations and
fieldtrips cannot be made up.
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Last day to withdraw: Make sure you note the date. It is the student’s responsibility to make sure they
withdraw. AW will not be given in lieu of forgetting to withdraw in time.
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Cellular Phones and Pagers Turn them off during lecture and laboratory! If for some reason you expect an
emergency situation to arise, please turn off the ringer and take the call outside of class. Please be
respectful of other students. Cell phones MUST NOT ring or be answered during laboratories or
exams!
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Internet Accessibility: All TCC students have access to computers and the Internet in the General User
Computer Labs on all four campuses. Public libraries are an additional location.
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Inappropriate Behavior in the Classroom – Increasingly many students display inconsiderate behavior
towards the instructor and other students. This will not be tolerated. Initially the instructor will speak to the
student individually. If a change in behavior is not observed, the student will be dropped from the class.
Such behavior includes disruptive late arrivals (repeated arrivals more than 7 minutes after a session
begins without prior notification is considered disruptive), excessive talking during lecture, repeated
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
leaving and returning to the room during lecture, repeated cell phone interruptions, and rudeness towards
the instructor.
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Changes in this Syllabus The policies and statements contained in this syllabus may be altered, added to,
or deleted at any time by the instructor of the class. This will be done by typed supplements or amendments
given to the student and/or posted on blackboard.
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Course Withdrawal: The deadline to withdraw from a course shall not exceed 3/4 the duration of any class.
Contact the Counseling Office at any TCC campus to initiate withdrawal from a course ('W' grade) or to
change from Credit to Audit. Check the TCC Academic Calendar for deadlines. Students who stop
participating in the course and fail to withdraw may receive a course grade of “F,” which may have financial
aid consequences for the student.
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Communications:
a. Email communications: All TCC students receive a designated “MyTCC” email address (ex:
jane.doe@mail.tulsacc.edu). All communications to you about TCC and course assignments will be
sent to your MyTCC email address; and you must use MyTCC email to send email to, and receive
email from, the instructor regarding this course.
b. Inclement Weather: TCC rarely closes. If extreme weather conditions or emergency situations arise,
TCC always gives cancellation notices to radio and television stations. This information is also posted
on the TCC website (www.tulsacc.edu).
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General Education Goals: General Education courses at TCC ensure that our graduates gain skills,
knowledge, and abilities that comprise a common foundation for their higher education and a backdrop for
their work and personal lives. TCC’s General Education goals are: Critical Thinking, Effective
Communication, Engaged Learning, and Technological Proficiency.
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Classroom Etiquette: Open and mutually respectful communication of varied opinions, beliefs, and
perspectives during classroom or online discussion encourages the free exchange of ideas that is essential
to higher learning and to the ability to learn from each other. Use of any electronic device (including
computers, ipads and smartphones) is at the discretion of the instructor.
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Academic Dishonesty: Academic dishonesty (cheating) is defined as the deception of others about one’s
own work or about the work of another. Academic dishonesty or misconduct is not condoned or tolerated at
campuses within the Tulsa Community College system. Tulsa Community College adopts a policy
delegating certain forms of authority for disciplinary action to the faculty. Such disciplinary actions delegated
to the faculty include, but are not limited to, the dismissal of disrespectful or disorderly students from
classes. In the case of academic dishonesty a faculty member may:
o require the student to redo an assignment or test, or require the student to complete a substitute
assignment or test;
o Record a "zero" for the assignment or test in question;
o Recommend to the student that the student withdraw from the class, or administratively withdraw
the student from the class;
o Record a grade of "F" for the student at the end of the semester.
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Faculty may request that disciplinary action be taken against a student at the administrative level by
submitting such request to the Dean of Student Services.
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
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It is the student’s responsibility to ensure that they understand the meaning of plagiarism, how to
avoid it and the consequences of any acts of plagiarism. Not understanding how to properly cite
materials or use citation format does not provide an excuse for academic dishonesty.
DISABILITY RESOURCES
It is the policy and practice of Tulsa Community College to create inclusive learning environments. Accommodations
for qualifying students in compliance with the Americans with Disabilities Act (ADA) and Section 504 of the
Rehabilitation Act are available. To request accommodations, contact the Education Access Center (EAC) at
eac@tulsacc.edu or call (918) 595-7115 (Voice). Deaf and hard of hearing students may text (918) 809-1864.
Blackboard is an accessible learning management system. Blackboard’s accessibility information can be found at
http://www.blackboard.com/Platforms/Learn/Resources/Accessibility.aspx .
Institutional Statement
Each student is responsible for being aware of the information contained in the TCC Catalog, the TCC Student
Policies & Resources Handbook, and semester information listed in the class schedule. All information may be
viewed on the TCC website: www.tulsacc.edu
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
FALL 2014 SCHEDULE OF READINGS AND ASSIGNMENTS
Week of
Lecture & Exams
Aug 18 
First day 
of class
Aug 25 
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Sept 1 
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Sept 8 
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Sept 15 
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Sept 22 
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Sept 29 
Oct 6 
Laboratory & Assignments
ADDITIONAL READINGS, QUIZ
Intro & Ubiquity
 Lab safety & introduction
 Basic Lab techniques
 Ubiquity (2.1)
 Aseptic Transfer (1.3)
Microscopy
 Microscopy (3.1)
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All items, including labs must be
submitted by Sept 1 at 5:30pm CST
Stains & Smears
 Simple Stain (3.4)
 Gram Stain (3.6)
 Acid Fast (3.7)
 Capsule Stain (HO; 3.8)
 Endospore Stain (3.9)
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All items, including labs must be
submitted by date Sept 8 at 5:30 pm
CST
Exam 1 – Chpt 4-6
(M)
Chp 7 – Microbial
Genetics
Aerotolerance
 Fluid Thioglycollate (2.6)
 Anaerobic Jar (2.7)
 Nitrate Reduction Test (5.6)
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Quiz 2 Chpts 7,8, & 13 (due Oct 29
at 5:30 pm CST)
All items including labs must be
submitted by Sept 15 at 5:30 pm
CST
Chp 8 – Recombinant
DNA Technology
Chp 13 - Viruses
Quiz & Unknown
 Lab Quiz 1 (W)
 Stain Unknown
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All items, including labs must be
submitted by Sept 22 at 5:30pm
CST
Review for exam 2
(M)
Exam 2 – Chpt
7,8,&13 (W)
Chp 15 – Innate
Immunity
Stain Unknown
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All items, including labs must be
submitted by Sept 29 at 5:30pm
CST
Selective and Differential Media
 Mannitol Salt (4.1)
 EMB (4.4)
 Hektoen (4.5)
 Unknown
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Quiz 3 Chpt 15-18 (due Oct 27 at
5:30 pm CST)
All items, including labs must be
submitted by Oct 6 at 5:30pm CST
Carbohydrates and Fermentation
 Phenol Red Broth (5.2)
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Syllabus
Chp 4 – Microscopy ,
Staining and
Classification
Chp 5 - Microbial
Metabolism
Chp 6 – Microbial
Nutrition & Growth
Labor Day (M) no
class
Review for exam 1
Chp 16 – Adaptive
Immunity
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Quiz 1 Chpts 4-6 (due Sept 8th at
5:30 pm CST)
All items, including labs must be
submitted by Aug 25 at 5:30pm CST
All items, including labs must be
submitted by Oct 13 at 5:30pm CST
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
Week of
Lecture & Exams
Oct 13 
Chp 17 –
Immunization and
Immune Testing
Oct 20 
Chp 18 – AIDS and
Immune Disorders
Oct 27 
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Review for exam 3
Exam 3 – Chpt
15,16,17, 18 (W)
Nov 3 
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Nov 10 
Nov 17 
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Nov 24 
Chp 9 – Controlling
Microbial Growth Environment
Chp 10 – Controlling
Microbial Growth Body
Chp 14 – Infection,
Infectious Disease
and Epidemiology
Review for exam 4
Exam 4 – Chpt
9,10,14 (W)
Review for Final
Exam
Laboratory & Assignments
 MR-VP (5.3)
 Unknown
Quiz & Respiration &Combination
Differential
 Lab Quiz 2 (W)
 Lysine Iron (5.22)
 Catalase (5.4)
 Oxidase (5.5)
 SIM (5.17)
 Unknown
Enzymes
 Starch (5.11)
 Gelatinase (5.14)
 Lipid (5.16)
 Unknown
Antimicrobials and Immunodiffusion
 Kirby-Bauer (7.2)
 Blood Typing (8.6)
 Hand out
 Unknown
Medical Micro
 Snyder (7.1)
 UV Light (2.11)
 Chemical Germicides (2.12)
 Blood Agar (5.20)
Quiz & Epidemiology & Bioterrorism
 Lab Quiz 3 (W)
 Epidemic (7.4)
 Bioterrorism - CDC film History of
Bioterrorism
 Unknown
 Epidemiology Simulation Game
Eukaryotic Pathogens & Presentations
 Eukaryotic Pathogens (HO)
 Presentations
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ADDITIONAL READINGS, QUIZ
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All items, including labs must be
submitted by Oct 20th at 5:30pm
CST
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All items, including labs must be
submitted by Oct 27th at 5:30pm
CST
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Quiz 4 Chpt 9,10,14 (due Nov 17 at
5:30 pm CST)
All items including labs must be
submitted by Nov 3rd at 5:30 pm
CST
All items, including labs must be
submitted by Nov 10 at 5:30pm CST
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Papers due Nov 17th at 5:30 pm
CST
All items, including labs must be
submitted by Nov 17 at 5:30pm CST
Unknown Report due Nov 24th at
5:30 pm CST
All items, including labs must be
submitted by Nov 24th at 5:30 pm
CST.
Thanksgiving Break – no lab
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Biology 2164 – Microbiology
Fall 2014
Tulsa Community College
Metro Campus
Week of
Lecture & Exams
Dec 1 
Dec 8
Review Final Exam
Laboratory & Assignments
 Lab Final Exam
FINALS & SEMESTER ENDS
ADDITIONAL READINGS, QUIZ
FINAL EXAM – Includes all chapters and
laboratory work assigned
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