Mitosis Question Booklet Name………………………………….….. Target Grade………………….. Grade achieved………………. __/27 __% Unit 2- Woodford County High School Question 1 Unit 2- Woodford County High School Unit 2- Woodford County High School Unit 2- Woodford County High School Question 2 The diagram below shows the structure of a chromosome as it might appear at the end of prophase of mitosis. A B (a) Name the parts labelled A and B. A ................................................................................................................................. B ................................................................................................................................. (2) Unit 2- Woodford County High School (b) During metaphase of mitosis, the chromosomes become attached to the equator of the spindle. Name the stage of mitosis that follows metaphase and describe the events that occur in this stage. Stage ........................................................................................................................... Events occurring ........................................................................................................ .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (3 (c) Explain the significance of the stage you have named and described in (b). .................................................................................................................................... .................................................................................................................................... (1) (d) Mitosis forms part of the cell cycle. Name one other stage of the cell cycle and state what occurs in the stage that you have named. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (Total 8 marks) Unit 2- Woodford County High School Question 3 An investigation was performed to determine the length of time that a cell in an onion root tip spends in each stage of mitosis. A growing root from an onion was selected and a root tip squash was made. This was examined under a light microscope and the percentage of cells in each stage of mitosis was determined. The results are shown in the table below. Stage of mitosis Percentage of cells in this stage Prophase 2.43 Metaphase 1.40 Anaphase 0.70 Telophase 2.78 (a) Describe how you would prepare a root tip squash so that mitosis could be studied. ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... ....................................................................................................................................... (4) Unit 2- Woodford County High School Mark Scheme Question 1 Question 2 (a) A - chromatid; B- centromere; (b) 2 Anaphase; Chromatids separate / centromere splits Move / pulled to (opposite) poles / ends of cell / ends of spindle to centrioles ; (c) By spindle fibres / microtubules 3 Daughter cells genetically identical (to parent cell) /maintains chromosome number/eq; 1 (d) Interphase / G1 / S / G2 / cytokinesis / cleavage If interphase or named stage - growth/synthesis of organelles Unit 2- Woodford County High School synthesis / replication of DNA / division of organelles OR If cytokinesis - division of the cytoplasm / formation of cell plate in plants ; Points linked. [if give ‘telophase’and then describe division of cytoplasm allow second mark] 2 [8] Question 3 (a) 1. Reference to named stain (acetic orcein / acetocarmine / Feulgens / Schiffs); 2. {Warm / heat} (with the stain / acid); 3. Break open tip (with needle / eq); 4. {Mount / eq} in {stain / acid / water / glycerol}; 5. (Gently) squash under coverslip / eq; 6. Warm (gently to intensify stain); 4 (b) 1. Telophase is the longest stage; 2. Anaphase is the shortest stage; 3. Correct manipulation of figures to compare any two phases; 4. Any two actual times given e.g. {prophase 29.16 mins / metaphase 16.80 mins / anaphase 8.40 mins / telophase 33.36 mins}; 3 (c) (Use the equation to) work out actual time of each phase; Total all four times; OR Add up all 4 percentages; {Substitute into equation / eq} / Multiply by 1200 ; 100 2 [9] Unit 2- Woodford County High School