MICROBIOLOGY SYLLABUS Instructor: Jessica Friel Phone 503.316.3202 E-mail:jessica.friel@chemeketa.edu Office Hours: Tuesday 3:30-4:30 in Room 1/308 Class Location: Building 1, Rm 308 Website: http://jfriel.wikispaces.com/BI234 COURSE DESCRIPTION: BI234 explores the world of bacteria and other microorganisms emphasizing their impact upon human health. Includes discussion of microbial structure and function, infection, immunity, common pathogens and methods of microbial identification and control. REQUIRED TEXTS & MATERIALS: 1. 2. 3. 4. Cowen. Microbiology Fundamentals: A Clinical Approach BI234 Lab Manual, VanHouten (buy at bookstore) Colored pencils Personal Protection Equipment (goggles or glasses--NO contacts, gloves, lab coat) All of these are available in the Chemeketa Bookstore or they may be purchased online at http://bookstore.chemeketa.edu/home.aspx COURSE OUTCOMES: Upon successful completion of this course, students should be able to: 1. Compare and contrast the various kinds of pathogenic microorganisms including bacteria, viruses, protozoans, fungi, helminths and insects. 2. Describe how microbes are named and classified and demonstrate the importance of these concepts to laboratory identification and microbial control. 3. Describe and explain the major features of bacterial structure, morphology, metabolism, growth and genetics and relate these to disease, identification, treatment and control. 4. Compare and contrast the major features of selected microbial disease including: identification of the etiologic agent, life cycle, transmission, symptoms, treatment and prevention and relate these features to the progression and control of disease. 5. Evaluate possible pathogens based on epidemiological evidence in case study form. 6. Demonstrate and apply the major principles, techniques and agents (chemical and physical) of microbial control. 7. Perform and use laboratory techniques designed to isolate, grow, maintain and identify microbes under controlled situations including: microscopy, aseptic technique, culture techniques and microbial control. 8. Define and apply basic principles related to infection and disease, including: modes of invasion and establishment, factors affecting pathogenicity and development of disease, and reactions of the human body to invasion and tissue damage. 9. Describe the development of immunity and the nature of vaccines and immunization. 10. Predict the presence of microbial populations, formulate solutions to control these populations and educate the public on basic issues in microbiology and immunology. PREREQUISITES: Required: BI231 and CH104 or CH110 Recommended: BI102, CH100, 105, 106, BI232 and BI233 It is expected that you have a basic understanding of chemical principles before you enter this class. In addition, anatomy and physiology are strongly recommended as a foundation for understanding human body function before, during and after microbial invasion. GRADING: Assessment Tool Laboratory Notebook Mystery Microbe Project Disease Presentation Midterms (2@100pts) Final Exam-Comprehensive Points ~76 ~20 30 200 200 Total (Tentative)--Total Points will fluctuate during the quarter ~526 Grade Scale 90%-100% 80-89% 70-79% 60-69% 59% and below Grade A B C D F NOTE: There are no make-up labs. If you miss more than 2 labs you will NOT receive credit for the course. No make-up quizzes or exams will be given, except under prearranged or special circumstances. Assignments turned in late will be marked down 50% per week. If you have a grade dispute, please submit a hard copy or e-mail explanation of your dispute and I will reply to you within 48 hours. ACCESSING YOUR GRADE THROUGHOUT THE TERM: When you would like to see your grade email me and ask to see your grade report. I will reply within 1 business day with your current scores. COURSE REQUIREMENTS: Lecture Material--Lecture material will be presented in the form of PowerPoint lecture notes, class activities and online class discussion. You will find the Power Point lecture notes on the course web page. Laboratory Notebook--A laboratory notebook will serve as your record of events that occurred in the microbiology lab. It will be a place to outline your experiments, record data and present your conclusions about your experiments. For those students going into an Allied Health field, the laboratory notebook will help you develop organizational skills that will be necessary when documenting patient records. Laboratory Notebooks will be graded for their completeness, accuracy and neatness. Mystery Microbe Project--The unknown bacteria identification project is a way for you to become a microbe detective. Think of yourself as a lab technician who receives a patient sample and you must identify the specimen accurately to offer the patient the best treatment plan. You will perform experiments, collect data, analyze data and draw conclusions in order to identify your unknown bacterial species. You will receive a numbered tube the second week in lab and then perform experiments with your unknown each week. At the end of the quarter you should be able to correctly identify your unknown bacterial genus and species. Disease Presentation Project--Individuals or pairs of students will investigate the current literature and websites about a diseases. Guidelines for these disease presentations will be discussed in class and posted on our class website. Midterms--Midterms will take place during the first hour of class. They will be taken in the lab using a printed, hard copy exam. Midterms will be in the form of short answer, multiple choice, true/false, short essay, long essay and case study questions. Final Exam--Like the midterms the final will also be a printed, hard copy exam. The final exam is cumulative meaning that all chapters will be fair game on the exam. A cumulative final allows you to review the class material and further store concepts into your long term memory. Don't let this scare you too much as students have performed similarly to their exams. You will be allowed to bring a "reference sheet" consisting of a single sided 8.5 x 11 in. COURSE RESOURCES AND POLICIES: Resources at Chemeketa— Please take advantage of Chemeketa's many resources!! :) You will notice direct links to some of these services at the bottom of our course content page and all the following services can be found on the starting course list page in Campus Edition 6: STUDENT SERVICES: Students with special needs should contact Disability Services (503.399.5192) for appropriate documentation of necessary accommodations. If you have emergency medical information or need special arrangements, please notify the instructor as soon as possible. The following is a list of services that are available to students at CCC: Computer Labs: Building 1/202; 503.316.3238; http://www.chemeketa.edu/locations/yamhill/services/computerlabs.html Counseling and Financial Aid: Building 1; 503.472.9482 http://www.chemeketa.edu/locations/yamhill/gettingstarted/advising.html Disability Services: 503.399.5192 http://www.chemeketa.edu/locations/yamhill/services/disability.html Language Center (Salem): Building 3/257; 503.399.5290 http://www.chemeketa.edu/earncertdegree/international/english/ Library, Tutoring, Study Skills and Writing help: Building 1/200; 503.316.3238 http://www.chemeketa.edu/locations/yamhill/services/tutoring.html Testing Services Building 1/203; 503.316.3240 or 503.316.3284 http://www.chemeketa.edu/locations/yamhill/services/testing.html Student Life Building 1 http://www.chemeketa.edu/locations/yamhill/services/clubs.html ACADEMIC INTEGRITY: ACADEMIC HONESTY STATEMENT: http://www.chemeketa.edu/earncertdegree/advising/studyskills/writing/resources/academichonesty/ Learning is built on the fundamental qualities of honesty, fairness, respect and trust. At Chemeketa Community College, academic integrity is a shared endeavor characterized by truth, personal responsibility and high academic standards. Any violation of academic integrity devalues the individual and the community as a whole. Violations of academic honesty include but are not limited to the following: plagiarism, cheating, collusion, fabrication / falsification /alteration, unauthorized submissions, and sabotage / tampering. Definition of Plagiarism: Plagiarism is defined as representing the words, ideas, or work of another as one’s own in any academic exercise. Plagiarism consists of taking the words or substance of another work and either copying or paraphrasing without giving credit to the source. Plagiarism is applicable to written, oral, and artistic work. The following examples are only some of the many forms plagiarism may take: 1. 2. 3. 4. Word-for-word copying of work written by someone else. Failure to give proper credit for ideas, statements of facts, or conclusions derived by another. Failure to use quotation marks when quoting directly from another, whether a paragraph, sentence, or phrase. Close and extended paraphrasing of another work without acknowledging the source. Definition of Cheating: Cheating is the act of obtaining or attempting to obtain credit for academic work through the use of dishonest, deceptive, or fraudulent means. The following are only some of the many forms cheating may take: 1. 2. 3. 4. 5. Copying another’s work on a test, paper, or project. Using unauthorized materials in an exam or collaborating on work to be turned in for credit where the instructor disallows such collaboration. Taking an exam for another student, purposely allowing another student to copy during a test, or providing coursework for another student to turn in as his or her own effort. Fabricating, falsifying or misrepresenting data or results from experiments, interviews or surveys. Submitting the same work in more than one class for credit without permission from the instructor. By taking this course, you agree to adhere to the Academic Honesty Guidelines. Infractions, such as cheating or plagiarism, WILL NOT BE TOLERATED and will be dealt with according to the policies of CCC. Any such infractions will constitute a violation of the college policy and will result in an appropriate consequence (e.g., zero on the assignment, an F for the course, etc.) at the discretion of the instructor and college officials. TECHNOLOGY RECOMMENDATIONS AND RESOURCES: Information Technology Help Desk at (503)399-7899 or Distance Education at (503)399-5191. Technology Recommendations Specific to this Course: 1. MS Word (if you do not have MS Word, please access a computer that does to download and print the Study Guide assignments and then complete them by hand.) 2. Power Point (if you do not have PowerPoint you can view lecture notes using the pdf files and Adobe Acrobat. For our disease project where Power Point is required you may need to complete your project using the Computer Lab found on the second floor of Building 6 on campus.) 3. Adobe Acrobat available as a free download and used to view the pdf files. CLASSROOM RESPONSIBILITY: Full participation is expected of each student. All class and laboratory assignments must be completed individually and originally unless otherwise stated. If you miss a class, it is your responsibility to determine material missed, obtain handouts, and be aware of the class schedule. The ultimate basis for determining acceptable behavior and procedure in this class will be the Chemeketa Creed (printed in the college catalog) http://www.chemeketa.edu/ Basically, respect others as you would have them respect you. CHEMEKETA'S DIVERSITY STATEMENT: Chemeketa is a community college enriched by the diversity of our students and staff. Each individual and group has the potential to contribute in our learning environment. Each has dignity. To diminish the dignity of one is to diminish the dignity of us all. (from the college catalog) http://www.chemeketa.edu/ HOW MUCH TIME DO I NEED TO DEDICATE TO MICRO? Be prepared to spend at least 2 hours outside of class for every hour inside of class. You will be in class 6 hours a week and expected to study at least 12 more hours during the week. Times may vary from week to week. To prevent the amount of class time from getting overwhelming a great idea is to schedule shorter blocks of time into your schedule on a regular basis. For example, plan to study for 3 hours 5 days a week. You can even split that time up into 3 1 hour blocks to make it fit in to your schedule. Procrastination is a very bad option in this class since if you get behind it is extremely difficult to catch up so organize your schedule in advance! TIPS FOR SUCCESS: 1. Start with the "big picture" of each chapter by reading the Chapter Summary found at the end of each chapter. 2. Then, start filling in the "details" by completing the study guide assignments in a timely manner. 3. Assess your knowledge using the self-quizzes at the end of the chapter and the LearnSmart study modules on the textbook website. 4. Establish study partners with whom to study and discuss issues in microbiology. 5. Review each week's material and try to apply it to your everyday life. Note: Please contact me ASAP if you are having difficulties with this class. Tentative Schedule for W 2014: Week Date M 1/6 1 W 1/8 M 1/13 2 W 1/15 3 4 5 M 1/20 off W 1/22 M 1/27 W 1/29 M 2/3 W 2/5 M 2/10 6 W 2/12 7 M 2/17 off Readings Due Introduction to Microbes and Their Building Blocks (Ch. 1) Tools of the Laboratory: The Methods for Studying Microorganisms (Ch. 2) Prokaryotic Structure Ch. 1,2 Study (and Function) Guides (Ch. 3) Eukaryotic Structure and Function (Ch. 4) Interactions Between Microbes and Humans (Ch. 11) Microbial Nutrition and Growth (Ch. 6) Microbial Metabolism (Ch. 7) Microbial Genetics and Genetic Engineering (Ch. 8) Diagnosing InfectionsPhenotypic methods (Ch. 15.1-15.4) Ch. 3,4 Study Guides Ch. 11 Study Guide Ch. 6 Study Guide Lab Notebook Ch. 7 Study Guide Disease presentation emailed to me by beginning of lab Class and Lab Activities Lab Safety Lab Notebook and Lab Methods and Materials Inoculate thumb TSB tubes Inoc environmental TSA plates Microscope Introduction Microscopic Observation of cells from different Kingdoms Observe thumb TSB Observe environmental TSA plates Intro Mystery Microbe Project Obs. unknown TSB Intro aseptic technique & Quad or T- Streak Inoc 2 TSA plates w/MM and incubate at 2 temps(RT=22Cand BT=37C) Intro Smear Prep and Gram stain Perform gingival smear Gram stain gingival & bacterial cells from gum smear Observe, compare, and contrast eukaryotes & prokaryotes Assign disease presentations Observe and Conclude 2 TSA plates inc'd at 2 difft temp Inoc Differential & Selective Media (Sheep Blood, MacConkey, MSA) Epidem Lab (Handout) Finish gingival gram stain and Kingdom slides EXAM #1 on 1/27 chapters 1, 2, 3, 4, and 11 Answer differential and selective media lab questions OBS and form conclusions for Differential & Selective Media (Sheep Blood, MacConkey, MSA) Inoc anaerobe vs. aerobe TSA Gram Stain unknown, E. coli and S. epiderm Answer anaerobe lab questions OBS and form conclusions for anaerobe vs. aerobe TSA Oxidase expt Observations and Conclusions Catalase expt Observations and Conclusions Complete Metabolism Worksheets DNA activity Genetics Activity Handout Genetic Engineering Activity using Paper plasmid Discuss MM identification process(start MM log & descriptive chart) Inoc 1 MRVP tube Viral Structure and Life Cycles (Ch. 5) Ch. 8 and 15.1-15.4 Study Guides W 2/19 M 2/24 8 W 2/26 M 3/3 9 W 3/5 M 3/10 10 W 3/12 Physical and Chemical Control of Microbes (Ch. 9) Antimicrobial Treatment (ch. 10) EXAM #2 on 2/19 chapters 6, 7, 8, and 15.1-15.4 Inoc Motility Media Inoc API 20E test Ch. 5 Study Guide Obs and form conclusions for GloGerm Handwash lab Observe and form conclusions for Motility Media Motility Determination using Hanging Drop technique OBS and form conclusions for MRVP tube Read API20E test strips Ch. 9 Study Guides Inoc Hand Washing Expt Introduce (Take home and inoc) Home Disinfection plate -2x weekly labs Disease presentation chart Host Defenses II: Review Exams for comprehensive final exam Specific Immunity & Obs and form conclusions for Hand washing Lab Immunization Ch. 10 Study (Ch. 13) Diagnosing Guide, InfectionsLab Notebook, Immunologic and Mystery methods Microbe (Ch. 15.5) report Final Monday Mar 17th at time of class, Ch. 13 and 15.5 Study Guide Comprehensive, can have a one sided 8.5 x 11 note card