INTRODUCTION TO MICROBIOLOGY SYLLABUS
Instructor: Jessica Friel Term: Summer 2015
Phone 503.316.3202
E-mail: jessica.friel@chemeketa.edu
Office Hours: by appointment
Website: http://jfriel.wikispaces.com/BI+230+Home+Page
COURSE DESCRIPTION:
BI230 explores the world of bacteria and other microorganisms emphasizing their impact upon society
including applications to winemaking. Includes discussion of the scope and history of microbiology,
chemistry relevant to microbiology, the diversity of microbial life, microbial structure and function,
infection, immunity, common pathogens and methods of microbial identification and control.
REQUIRED TEXTS & MATERIALS:
1.
2.
3.
4.
Bruce Hofkin. Living in a Microbial World, 1st Edition
BI230/BI234 Lab Manual by VanHouten (buy in the bookstore)
Colored pencils
Personal Protection Equipment (gloves, lab coat, goggles or glasses--NO contacts,)
All of these are available in the Chemeketa Bookstore or they may be purchased online at
http://bookstore.chemeketa.edu/home.aspx
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
COURSE OUTCOMES:
Upon successful completion of this course, students should be able to:
Compare and contrast the various kinds of pathogenic microorganisms including bacteria,
viruses, protozoans, fungi, helminths and insects.
Describe how microbes are named and classified and demonstrate the importance of these
concepts to laboratory identification and microbial control.
Describe and explain the major features of microbial structure, morphology, metabolism,
growth and genetics and relate these to disease, identification, treatment and control.
Compare and contrast the major features of selected microbial diseases including: identification
of the etiologic agent, life cycle, transmission, symptoms, treatment and prevention and relate
these features to the progression and control of disease.
Evaluate possible pathogens based on epidemiological evidence in case study form.
Demonstrate and apply the major principles, techniques and agents (chemical and physical) of
microbial control.
Perform and use laboratory techniques designed to isolate, grow, maintain and identify
microbes under controlled situations including: microscopy, aseptic technique, culture
techniques and microbial control.
Define and apply basic principles related to infection and disease, including: modes of invasion
and establishment, factors affecting pathogenicity and development of disease, and reactions to
invasion and tissue damage.
Describe the development of immunity and the nature of vaccines and immunization.
Predict the presence of microbial populations, formulate solutions to control these populations
and educate the public on basic issues in microbiology and immunology.
PREREQUISITES:
Recommended: BI102, CH110, 104, 105, 106. It is expected that you have a basic understanding of
chemical principles before you enter this class.
GRADING:
Assessment Tool
Study Guides (13 @ 10pts each)
Laboratory Notebook
Mystery Microbe Project
Final Project
Midterms (3 @ 100pts each)
Total
Grade Scale
90%-100%
80-89%
70-79%
60-69%
59% and below
Points
130
~76
~20
100
300
~626
Grade
A
B
C
D
F
ACCESSING YOUR GRADE THROUGHOUT THE TERM:
When you would like to see your grade email me and ask to see your grade report. I will reply within 1
business day with your current scores. I can only communicate grade information over MyChemeketa
email.
NOTE: There are no make-up labs. If you miss more than 2 labs you will NOT receive credit for the
course. No make-up quizzes or exams will be given, except under prearranged or special circumstances.
PUNCTUALITY and LATE ASSIGNMENTS:
Punctuality is crucial. Late assignments are generally not accepted with the exception of certain
circumstances (e.g., illness, emergency) or a late pass. You will be given one late pass per term, you
cannot use another person’s pass. Assignments are due at the beginning of the specified class session
(unless otherwise noted) and are considered late thereafter.
If you are late for an exam or quiz, you will have the remainder of the time given to the class for
completion. For example, if you are 10 minutes late to a 60 minute exam, you will have the remaining 50
minutes to complete the exam. Tardiness to class will not only affect your personal learning but also the
learning of group members. Given an acceptable excuse for their tardiness (e.g., emergency), late
individuals may be provided an alternate assignment. Please note that work/traffic/parking/etc. are not
acceptable excuses for tardiness.
COURSE MATERIALS AND ASSIGNMENTS:
Lecture Material--Lecture material will be presented in the form of PowerPoint lecture notes and class
activities. You will find the Power Point lecture notes on the course web page. I convert the files into a
.pdf file which is easily read by the free program Adobe Acrobat.
Study Guides--The Study Guides are the learning objectives for the week. If you are comfortable with
the information in the study guide questions, you will be on your way to a good grade on the exams
since these are the concepts I will assess you on. They are posted on the course website.
Laboratory Notebook--A laboratory notebook will serve as your legal record of events that occurred in
the microbiology lab. It will be a place to outline your experiments, record data and present your
conclusions about your experiments. Laboratory Notebooks will be graded for their completeness,
accuracy and neatness.
Mystery Microbe Project--The unknown bacteria identification project is a way for you to become a
microbe detective. Think of yourself as a lab technician who receives a patient sample and you must
identify the specimen accurately to offer the patient the best treatment plan. You will perform
experiments, collect data, analyze data and draw conclusions in order to identify your unknown
bacterial species. You will receive a numbered tube the second week in lab and then perform
experiments with your unknown each week. At the end of the quarter you should be able to correctly
identify your unknown bacterial genus and species.
Final Class Project--This will be a PowerPoint project in which you apply course concepts to your
personal choice of an area of microbiology. Guidelines for the project are on the course homepage and
we will discuss the guidelines as the quarter progresses.
Exams-- Exams will take place at the beginning of class. They will be in the form of short answer,
multiple choice, short essay, long essay and case study questions.
STUDENT SERVICES:
Students with special needs should contact Disability Services (503.399.5192) for appropriate
documentation of necessary accommodations. If you have emergency medical information or need
special arrangements, please notify the instructor as soon as possible.
The following is a list of services that are available to students at CCC:
Computer Labs: Building 1/202; 503.316.3238;
http://www.chemeketa.edu/locations/yamhill/services/computerlabs.html
Counseling and Financial Aid: Building 1; 503.472.9482
http://www.chemeketa.edu/locations/yamhill/gettingstarted/advising.html
Disability Services: 503.399.5192
http://www.chemeketa.edu/locations/yamhill/services/disability.html
Language Center (Salem): Building 3/257; 503.399.5290
http://www.chemeketa.edu/earncertdegree/international/english/
Library, Tutoring, Study Skills and Writing help: Building 1/200; 503.316.3238
http://www.chemeketa.edu/locations/yamhill/services/tutoring.html
Testing Services Building 1/203; 503.316.3240 or 503.316.3284
http://www.chemeketa.edu/locations/yamhill/services/testing.html
Student Life Building 1
http://www.chemeketa.edu/locations/yamhill/services/clubs.html
ACADEMIC INTEGRITY
ACADEMIC HONESTY STATEMENT:
http://www.chemeketa.edu/earncertdegree/advising/studyskills/writing/resources/academichonesty/
Learning is built on the fundamental qualities of honesty, fairness, respect and trust. At Chemeketa
Community College, academic integrity is a shared endeavor characterized by truth, personal
responsibility and high academic standards. Any violation of academic integrity devalues the individual
and the community as a whole. Violations of academic honesty include but are not limited to the
following: plagiarism, cheating, collusion, fabrication / falsification /alteration, unauthorized
submissions, and sabotage / tampering.
Definition of Plagiarism:
Plagiarism is defined as representing the words, ideas, or work of another as one’s own in any academic
exercise. Plagiarism consists of taking the words or substance of another work and either copying or
paraphrasing without giving credit to the source. Plagiarism is applicable to written, oral, and artistic
work. The following examples are only some of the many forms plagiarism may take:
 Word-for-word copying of work written by someone else.
 Failure to give proper credit for ideas, statements of facts, or conclusions derived by another.
 Failure to use quotation marks when quoting directly from another, whether a paragraph,
sentence, or phrase.
 Close and extended paraphrasing of another work without acknowledging the source.
Definition of Cheating:
Cheating is the act of obtaining or attempting to obtain credit for academic work through the use of
dishonest, deceptive, or fraudulent means. The following are only some of the many forms cheating may
take:
 Copying another’s work on a test, paper, or project.
 Using unauthorized materials in an exam or collaborating on work to be turned in for credit
where the instructor disallows such collaboration.
 Taking an exam for another student, purposely allowing another student to copy during a test,
or providing coursework for another student to turn in as his or her own effort.
 Fabricating, falsifying or misrepresenting data or results from experiments, interviews or
surveys.
 Submitting the same work in more than one class for credit without permission from the
instructor.
By taking this course, you agree to adhere to the Academic Honesty Guidelines. Infractions, such as
cheating or plagiarism, WILL NOT BE TOLERATED and will be dealt with according to the policies of CCC.
Any such infractions will constitute a violation of the college policy and will result in an appropriate
consequence (e.g., zero on the assignment, an F for the course, etc.) at the discretion of the instructor
and college officials.
Tentative Schedule for Summer 2015:
Week
Date
Readings and Exams
T
6/23
Living in a Microbial
World (CH 1, pg. 114)
Microbiology and
History (CH 5, pg.
106-130)
R
6/25
The Cell: Where Life
Begins (CH 3, pg.

45-69, CH 8, pg.

210-213)
1
T
6/30
2
R
7/2
T
7/7
3
R
7/9
T
7/14
4
R
7/16
T
7/21
5
R
7/23
A Field Guide to the
Microorganisms
(Classification) (CH
4, pg.71-105)
Guess Who's
Coming to Dinner:
Microorganisms and
Food (CH 15, pg.
395-410)
EXAM #1
(CH: 1, 3, 4, 8, 15.
Everything on Study
Guides 1, 2, 3 and 4)
The Chemistry of
Life (CH. 2, pg 1728)
The Chemistry of
Life (CH. 2, pg 2942)
Metabolism and
Growth (CH 7, pg.
169-201)
Metabolism and
Growth (CH 7, pg.
169-201)
Better Living With
Microorganisms:
Industrial and
Applied
Microbiology (CH.
16, pg. 417-437)
Control of Microbial
Growth (CH 12, pg.
315-340)
An Ecologist's Guide
to Microbiology (CH
9, pg. 229-257)
Class and Lab Activities







Due





Lab Safety
Lab Notebook and Lab Methods and Materials
Inoculate thumb TSB tubes
Inoculate environmental TSA plates
Introduce final project
Microscope Introduction
Microscopic Observation of cells from different
Kingdoms
Introduce Smear Prep and Gram stain
Gram stain gingival & bacterial cells from gum
smear
Introduce aseptic technique & Quad or TStreak
Introduce Mystery Microbe (MM) Project
Choose MM groups
Inoculate MM TSA and incubate at 2 temps
Observe MM TSB
Inoculate MM Ox/Ferm Basal media tubes




Inoculate anaerobe vs. aerobe MM TSA
Observe MM TSA incubated at 2 temps
Inoculate MM Differential & Selective Media
Observe MM Ox/Ferm Basal media tubes
Study Guides 3
and 4




Oxidase MM observations
Observe anaerobe vs. aerobe MM TSA
Catalase MM observations
Observe and form conclusions for Differential &
Selective Media
Study Guide 5
and 6

 Gram Stain MM, E. coli and S. epiderm
 Inoculate MM MRVP tube
 Inoculate MM Motility Media
Study Guides 1
and 2
Study Guide 7
Lab Notebook
Final Project
Rough Draft
T
7/28
EXAM #2
(CH: 2, 7, 16, 12, 9.
Everything on Study
Guides 5, 6, 7, 8, 9)
Microbial Genetics
(CH 6, pg. 133-166)
R
7/30
Microbial Genetics.
Con’t. (CH 6, pg.
133-166)
6
T
8/4
7
R
8/6
T
8/11
8
R
8/13
The Future is Here:
Microorganisms and
Biotechnology (CH
14, pg. 367-393)
Microbial Evolution:
Antibiotic
Resistance (CH 8,
pg.218-220)
The Nature of
Disease: A
Pathogen's
Perspective (CH 10,
pg. 259-282)
Host
Defenses/Vaccines
(CH 11, pg. 285-290,
303-307)
Epidemiology: Who,
What, When,
Where and Why?
(CH 13, pg. 343-365)
N/A








Protein synthesis packet
Genetic Engineering Activity: Paper plasmid
DNA Fingerprinting (Demo)
Inoculate MM API 20E test strips
Inoculate Hand Washing Expt
Introduce (Take home) Home Disinfection plate
Observe and form conclusions for MRVP tube
Inoculate and form conclusions for SBA & MSA
Plates
Study Guides 8
and 9
 Observe MM Motility Media
 Observe and form conclusions for Hand
washing Lab
 Observe and form conclusions for GloGerm
Hand washing lab
 Observe Home Disinfection plate
 Observe and form conclusions for SBA & MSA
Plates
 Read API20E test strips
 Determine MM motility using Hanging Drop
technique
 Discuss identification process
Study Guide 10
 Review Exams
 Finish experiments (Open lab)
Study Guides 11
and 12
Mystery
Microbe report
Study Guide 13
Lab Notebook
EXAM #3 (CH: 6, 14, 8, 10, 11. Everything on
Study Guides 10, 11, 12, 13)
Final Project
For each exam you can have a full page of notes (written on one side)
The tests are not cumulative