Living Things Characteristics of Living Things: Defining life is complicated. No one characteristic definitively identifies an organism as living. Characteristics of Living Things: Some characteristics that living things share: 1) 2) 3) 4) 5) 6) 7) 8) 9) Made up of cells Reproduction Based on Genetic Code Metabolism Motility Responsiveness Development / Growth Living things Evolve Living things are Adapted Characteristics of Living Things: 1) Made up of cells: collection of living matter enclosed in a barrier that separates the cell from its surroundings Smallest unit of an organism that can be considered alive. They grow, respond to surroundings, and reproduce. Living organisms can consist of one cell (unicellular) Larger and more complex organisms such as animals are composed of many cells (multicellular) Cells can exist in many sizes, shapes, and have different functions. Ex) the human body is made up of over 85 different types of cells. Characteristics of Living Things: 2) Reproduction: 2 basic types Asexual: the new organism has a single parent. EX: single-celled organisms dividing in half to form two Sexual: two cells from different parents unite to produce the first cell of the new organism. Characteristics of Living Things: 3) Based on Genetic Code Explaining how organisms inherit traits is one of the greatest achievements of biology. We have used this information to diagnose diseases, to identify suspects, and in determining paternity. The directions for inheritance are carried on a molecule called DNA. Characteristics of Living Things: 4) Metabolism Sum of all chemical reactions within a cell or organism. These chemical reactions will require energy. Organisms must get energy from the environment either by eating food and then breaking it down or using sunlight to create food and then breaking it down. Characteristics of Living Things: 5) Motility Self –propelled motion. This helps organisms collect food and escape predators ** remember not all living things posses all 9 characteristics. Characteristics of Living Things: 6) Responsiveness Responding to stimuli is one of the most basic properties of life. Stimuli can be anything from chemicals to temperature to touch as long as it the presence of the stimuli created a response. Characteristics of Living Things: 7) Development / Growth Ordered sequence of progressive changes that result in increased complexity Increase in size Development of new physical structures Refinement of reasoning and behavior Characteristics of Living Things: 8. Living things Evolve Living things adjust to environmental changes through biological changes over time Evolution provides new ways for organisms to use energy, maintain order, and perpetuate themselves. Occur slowly over the course of many generations Characteristics of Living Things: 9. Living things are Adapted Specific structures and behaviors that allow organisms to survive in its own environment Increase the individual’s ability to combat disorganization and improve the chance of perpetuating into the future Life Processes Nutrition – use of nutrients by an organism Digestion – process that breaks down large food molecules into forms that can be used by the cell Absorption – ability of a cell to take in nutrients, water, gases, and other substances into and out of the cell Transport – movement of nutrients, water, gases, and other substances into and out the cell Life Processes Biosynthesis – cellular process of building new chemical compounds for the purpose of growth, repair, and reproduction Homeostasis – ability of an organism to maintain a steady internal state regardless of external influence Life Processes Secretion – release of substances from a cell Photosynthesis – cellular process in which a plant makes food from water and carbon dioxide using energy from the sun Respiration – release of energy from chemical breakdown of compounds within a cell Excretion – ability of a cell to rid itself of waste products Discovery of the Cell The People In 1665 Englishman Robert Hooke used a compound microscope to look at thin slices of cork. He saw thousands of small empty chambers he called "cells" after the small rooms of a monastery. The People In Holland around the same time Anton van Leeuwenhoek used a single-lens microscope to look at pond water. He saw hundreds of tiny living organisms. The People In 1838 German botanist Matthias Schleiden concluded all plants are made of cells. In 1839 German Biologist Theodor Schwann said cells also compose all animals. The People In 1855 German physician Rudolf Virchow stated all new cells were produced from the division of existing cells. These conclusions (confirmed by other biologists) make up the cell theory. The Cell Theory: 1) All living things are composed of cells 2) Cells are the basic units of structure and function in living things. 3) New cells are produced from existing cells. Exploring the Cell Compound light microscope: uses two magnifying lenses and a series of mirrors to magnify an object. The total magnification of looking through two lenses at once is found by multiplying the magnification of each lens together. Problem with light microscopes: limitation of detail or resolution because light radiation is diffracted (scattered) as it passes through matter. Electron microscopes can produce images 1,000 times smaller than light microscopes because the wavelength of electrons is shorter than that of light. Transmission electron microscopes (TEM): using a beam of electrons allows us to see cell structures and large proteins. However the beam can only pass trough thin samples so the object must be cut into ultra thin slices. Scanning electron microscopes (SEM): electrons are scanned over the object producing 3D image. Organisms don't have to be cut into thin stripes Problems with electron microscopes: Electrons are easily scattered by molecules in the air so they have to be used in a vacuum. As a result all objects have to be preserved or nonliving. Cell Types General Characteristics Cells can vary greatly in size from bacteria at 0.2 micrometers to amebas that can be seen with the unaided eye at 1000 micrometers. Typically cells fall between 5 and 50 micrometers Despite the variety of sizes, shapes, and functions all cells have two things in common. All cells are surrounded by a barrier called a plasma membrane All cells contain DNA Prokaryotic vs Eukaryotic Cells are divided into two broad groups based on whether or not they have a nucleus. A nucleus is a large membrane bound structure that contains genetic material (DNA). Prokaryoctic: cells that do not have a nucleus or any internal membranes. Eukaryotic: cells that contain a nucleus as well as other internal membranes that each perform a different function Prokaryotic Since these cells do not have internal structures that divide up tasks they must stay smaller and simpler. Contains genetic material not bound by a nucleus. This genetic material is normally in the form of rings called plasmids. Both All are surrounded by a cell membrane All contain DNA and RNA Some cells are capable of moving using cilia or flagella. All contain ribosomes to make proteins. All contain cytoplasm and a cytoskeleton. Some cells have a cell wall. Eukaryotic Generally larger and more complex Many of these cells are highly specialized to perform single tasks for the larger organism (cell specialization) Most contain dozens of structures bound by membranes called organelles All contain a nucleus which separates the genetic material from the rest of the cell. There is normally more DNA. Pieces of the Cell Organelles Found in Both Prokaryotic and Eukaryotic Cells • Plasma/cell membrane: regulates what enters and leaves the cell, provides protection, and support. • Cell Wall: present in most plants, some algae, fungi, and prokaryotic cells (NOT ANIMAL CELLS). Located outside the membrane. The cell wall provides support and protection. • Cytoplasm: Fluid that suspends the structures of the cell and aids in transport of materials • Ribosomes: Synthesis proteins. • Cilia: small hair like projections that help the cell move or move material across the membrane • Flagella: long tail like projects. Same function as cilia. Both are not normally found in plant cells. Organelles found in all Eukaryotic cells • Nucleus: contains almost all the cell’s DNA which controls cell functions (the mitochondria and chloroplast have their own DNA). The nucleus is surrounded by the nuclear envelope which is composed of two membranes full of nuclear pores that allow materials to move in and out of the nucleus. Most nuclei contain a dense region known as the nucleolus. This is where making ribosomes begins. • Rough Endoplasmic Reticulum: series of membranes that are covered in ribosomes. It receives instructions from DNA inside the nucleus, allows the ribosomes to make the proteins, and then transports them to the next destination. • Smooth Endoplasmic Reticulum: make lipids Organelles found in all Eukaryotic cells • Golgi apparatus/complex: stacks of membranes that modify, sort, and package proteins made in the rough ER for reactions or structures inside the cell, or to be secreted out of the cell • Lysosome: structure made by the golgi. They are filled with enzymes that break down lipids, carbohydrates, and proteins for the cell to use. They also break down old organelles and other waste to be excreted. • Vacuole: saclike structures used for storage of water, salt, and nutrients. In many plant cells there is one large central vacuole that is filled with liquid to provide structural support for heavy leaves and flowers Organelles found in all Eukaryotic cells • Mitochondria: converts chemical energy stored in food into a usable form of energy for the cell by using enzymes (made in the ER) and chemical reactions to break the food down. It is enclosed in two membranes and the inner membrane is folded to allow more surface area for the reactions. They also contain their own DNA Organelles Found in Animal Eukaryotic Cells • Centrioles: organelles that help the cell divide • Remember that animal cells can have cilia and flagella like a prokaryotic cell Organelles Found in Plant Eukaryotic Cells • Chloroplast: convert energy from the sun into chemical energy stored in the form of sugar through photosynthesis. Chloroplasts are surrounded by two membranes and contain DNA. After the food is created it will be moved to the mitochondria for breaking down. • Plant cells are also the only eukaryotic cells to have a cell wall. • Remember that the vacuole of plant (one large vacuole) looks different from the vacuole in an animal cell (many small bubbles) and has the added function of structural support. Cell Transport of Materials The Plasma Membrane Structures The plasma/cell membrane will control what enters and leaves the cell. In order to achieve this function it is made up of several structures. Lipids: the plasma membrane is primarily made of two layers of lipids (lipid bilayer) that maintain the boundary of the cell. Transport Proteins: imbedded in the membrane to create gaps in the membrane for larger molecules to pass through. Receptor Proteins: attached to receive messages from other cells in the form of hormones that communicate what the cell needs to be doing. Carbs: attached to the surface of the cell as a form of identification. The Plasma Membrane Functions The membrane is selectively permeable which means only certain things will be able to go through the membrane. Cells exist in a liquid environment (inside and out) so the materials that are going to be moved are dissolved or suspended in water There are two basic ways to move materials across the cell membrane: Passive transport moves materials from an area of high concentration to an area of low concentration and requires no energy. Active transport either moves extremely large materials that are not capable of going through the membrane or it move materials from low concentration to high concentration. This requires energy from the cell. Types of Passive Transport Diffusion: the movement of materials that can fit though the membrane from high to low concentration. This occurs because molecules are in constant motion. When they run into each other they will ricochet off and move in opposite directions causing them to spread out into an area of low concentration Osmosis: the diffusion of water Facilitated diffusion: a protein creates a gap in the membrane in order to allow larger particles to diffuse. These proteins are very specific to only one type of substance. Remember that all types of passive transport require no energy. Types of Solutions that Cause Osmosis Since water is small and can fit directly through the membrane while most molecules dissolved in water cannot, water can be moved in an effort to create equal concentrations on both sides of the membrane. When cells are placed in solutions that have different concentrations from the internal environment of the cell it creates osmotic pressure and the water will move through osmosis to restore equilibrium. Once an equal concentration is reached particles that can move through the membrane will continue to move because particles never stop moving. However there will be no change in the concentration as particles will move back and forth equally. This is dynamic equilibrium. Types of Solutions that Cause Osmosis If a cell is placed into a solution with the same concentration of dissolved particles as what is inside the cell, water will move in and out of the cell at a constant rate and the cell will not change. This type of solution is called an isotonic solution. Types of Solutions that Cause Osmosis If the cell is placed into a solution that has a higher concentration of dissolved particles than what is inside the cell, the water inside of the cell will move out of the cell and into the solution in an effort to dilute it so that the concentrations are equal. This will cause the cell to shrink. This type of solution is called a hypertonic solution. Types of Solutions that Cause Osmosis If the cell is placed into a solution that has a lower concentration of dissolved particles than what is inside the cell, the water in the solution will move into the cell in an effort to dilute it so that the concentrations are equal. This will cause the cell to swell and possibly burst. This type of solution is called a hypotonic solution. Types of Active Transport Active transport using proteins: transport proteins can act as pumps by changing shapes in order to force materials from an area of low concentration to an area of high concentration (against the concentration gradient) Endocytoses: the cell can engulf or wrap around particles that are too large to go through a protein. Two types: Phagocytosis: engulfs solid particles such as food Pinocytosis: engulfs liquids Exocytosis: releases materials from the cell by having a vacuole fuse with the membrane and release the contents to the outside.