CELLULAR TRANSPORT CELL TRANSPORT TERMS Concentration: amount of material per unit volume, or as a percentage Solution: one substance (solute) dissolved in another (solvent) eg. saltwater has salt (solute) dissolved in water (solvent) Diffusion: movement in a solution from an area of high concentration to an area of low concentration (different regions of concentration = concentration gradient) -material diffuses naturally by KMT Brownian movement: movement in straight line until collision, then movement in new straight line Osmosis: diffusion of solvent (usually water) through a membrane Dialysis: diffusion of solute (eg. salt, water) through a membrane Hypotonic solution: concentration of solute is lower in solution than in cell (eg. cell in fresh water: water will enter cell, salt will leave cell, cell gets bigger) Hypertonic solution: concentration of solute is higher in solution than in cell (eg. cell in salt water: water will leave cell, salt will enter cell, cell shrinks) Isotonic solution: concentration of solute inside the cell is equal to outside -diffusion will stop, as there is no concentration gradient Turgid: - describes a swollen cell filled with solvent (in hypotonic solution) -if a plant cell is turgid, the large vacuole remains filled, and the cell wall holds shape -if an animal cell is turgid, the cell swells, then explodes (lyses) due to lack of cell wall Turgor pressure: pressure of water pushing out on the cell membrane Osmotic pressure: pressure of water through the cell membrane Plasmolysis: cell membrane shrinking (in hypertonic solution) Passive Transport: movement through a membrane by diffusion, without use of cellular energy 1. Simple diffusion: some substances can diffuse through imperfections in the cell membranes phospholipid bilayer -this occurs to small uncharged (eg. oxygen) as well as polar compounds (eg. water) 2. Facilitated diffusion: diffusion of larger particles (eg. glucose) or charged atoms (eg. sodium ions) -this occurs through proteins in the phospholipid bilayer Active Transport: -movement through a membrane via proteins using the cells energy (ATP provides this energy) -this occurs against a concentration gradient (low to high conc.) or with gradient but quicker eg. sodium/potassium pump needs ATP to move sodium against a concentration gradient Large Scale Transport: -bulk movement of particles into vacuoles using ATP Endocytosis: -bulk movement into cells a) Phagocytosis: “cell eating” -cell reaches out with pseudopods (cell arms) -food is surrounded in a vacuole this way -lysosomes add digestive enzymes to vacuole (eg. white blood cells) b) Pinocytosis: “cell drinking” -liquid is ingested as cell membrane pinches in with small vacuoles c) Receptor Mediated Endocytosis: -particles land on receptors on cell membrane -the receptors clump together and are engulfed in a vacuole Exocytosis: -bulk movement out of cells -vacuole (or vesicle) merges with cell membrane, releasing contents PHOTOSYNTHESIS Photosynthesis: -chemical reactions which make sunlight energy into stored chemical energy (sugar) -occurs in green chlorophyll in chloroplasts of plants Chloroplast structure: -have double membrane -fluid stroma -stacks of chlorophyll containing lamella called grana -space called intergrana in between grana two reactions occur: light-dependent reactions:(occurs in thylakoid membranes) -water (with red and blue light) is turned into oxygen and hydrogen light-independent reactions:(occurs in stroma) -hydrogen and carbon dioxide make glucose (sugar) word equation: red and blue light + carbon dioxide + water ---chlorophyll---> oxygen + glucose symbol equation: red and blue light + 6CO2 + 6H2O ---chlorophyll---> 6O2 + C6H12O6 (stored chemical energy) REACTANTS PRODUCTS Autotrophs: -organisms that produce their own food (eg. plants ---> with chloroplasts, algae, some bacteria) chemosynthesis: -some bacteria make ATP from sulphur and iron containing compounds AEROBIC CELLULAR RESPIRATION Aerobic Cellular Respiration: -stored chemical energy (sugar) is broken into useful stored chemical energy (ATP) using oxygen ATP = adenosine triphosphate -ATP is an easy to use chemical energy, used for life functions ADP + energy + phosphate <----> ATP (energy now stored in bonds) -energy is released as ATP breaks into ADP and P (exergonic reaction – energy is lost) -building ATP is an endergonic reaction as energy is stored -partially occurs in mitochondria mitochondria structure: - outer and inner membranes -a folded inner membrane(called a cristae) -a fluid called matrix in the middle -three reactions occur: 1. Glycolysis (in cytoplasm): 1 glucose makes 2 ATP 2. Kreb’s cycle (in matrix of mitochondria): oxygen is used, 2 ATP and carbon dioxide are made 3. ETC (in inner membrane of mitochondria): water is made, as well as 32 ATP word equation: 36 ADP + 36 P + glucose + oxygen ---enzymes-> water + carbon dioxide + 36 ATP symbol equation: 36 ADP + 36 P + C6H12O6 + 6 O2 ---enzymes->6 H2O + 6 CO2 + 36 ATP ENZYMES Enzymes are “organic catalysts” Catalyst: -chemical that reduces energy needed to start reactions (activation energy) eg. now reaction will occur at room temperature Organic: -from living things, containing C, H ANAEROBIC CELLULAR RESPIRATION Fermentation is also called anaerobic cellular respiration -stored chemical energy in glucose is converted into 2 ATP without the use of oxygen Comparison Point organisms first reaction formula Alcoholic Fermentation Lactic Acid Fermentation -bacteria, yeast -bacteria, animals -glycolysis -glycolysis glucose ---enzymes-> 2ATP + ethyl alcohol + CO2 glucose –enzymes-> 2ATP + lactic acid