Microbiology

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Microbiology

“scientific study of microorganisms and their effect on other living organisms”

Naming Microorganisms

• Page 266

• staphyl = “clustered together”

• coccus = “shaped like a sphere or ball”

• aureus = “a golden-yellow color”

• Staphylococcus aureus

Classification

(Page 268)

• Kingdom

• Division or Phylum

• Class

• Order

• Family

• *Genus

• *Species

• “King Phillip Came Over From Great Spain”

Staphylococcus aureus

• genus = Staphylococcus

• species = aureus

italics : not English

• genus: abbreviated e.g. S. aureus

Categories of Cells

• Page 267

• Eukaryotic (“True Nucleus”)

– nuclei and cytoskeleton

– DNA within the nucleus

• Prokaryotic (“Before Nucleus”)

– no nuclei and no cytoskeleton

– DNA float “freely”

– Bacteria and cyanobacteria

• Page 269

• Bacteriology

• Rickettsiology

• Virology

• Protozoology

• Mycology

Divisions

Bacteriology

• “science that studies bacteria”

Bacteria

• Page 269

• “a prokaryotic one-celled microorganism of the Kingdom Monera, existing as free living organisms or as parasites, multiplying by binary fission and having a large range of biochemical properties”

• pathogenic

Mycoplasmas

• Page 270

• “bacteria of the Mycoplasma genus that are found in humans and have no cell wall; the smallest free-living organisms presently known being intermediate in size between viruses and bacteria”

• double-stranded DNA

Chlamydia

• “a large group of nonmotile, gram negative intracellular parasites”

• replicate in cytoplasm of host cells

• use host’s ATP (Adenosine triphosphate) for energy

Rickettsiology

• “area of science that studies Rickettsia”

Rickettsia

• Page 270

• “a genus of gram-negative, pathogenic, intracellular parasitic bacteria”

• rod-shaped, sphere-shaped, change shape

• reproduce within host cell

• “arthropod vectors”

• tetracycline

• sulfonamides encourage growth

Virology

• Page 272

• “the study of viruses and viral diseases”

• Virus: “one of a group of minute infectious agents, with certain exceptions (e.g. pox viruses) not resolved in the light microscope, and characterized by a lack of independent metabolism and by the ability to replicate only within living host cells”

• rod-shaped, spherical, polyhedral, tad-pole

Virion

• individual particle that consists of nucleic acid

(the nucleoid), DNA or RNA (not both), and a protein shell (capsid)

• viruses do not have ATP

Groups of Viruses

• bacterial, animal, and plant

• classified according to:

– origin: reoviruses (respiratory and enteric)

– mode of transmission: arboviruses (mosquitoes)

– manifestations: (symptoms)polioviruses, poxviruses

– geographic location: coxsackievirus (names after

Coxsacki, New York)

Prion

• “small proteinaceous infectious agents

(particles) which almost certainly do not have a nucleic acid genome and thereby resist inactivation by procedures that modify nucleic acids”

• do not contain DNA or RNA

• spongiform encephalopathies

• eg: scrapie, mad cow, Creutzfeldt-Jakob

Protozoology

• Page 271

• “science that deals with the study of protozoa”

Protozoa

• “one celled organisms of the Kingdom

Protista- most are unicellular although some are colonists”

• “first animals”

• most abundant

• “normal flora”

• classified: according to shape and method of motility

Flagellates

• smallest

• whip-like projections (move like a fishtail)

Amoebae

• “ooze about” by extending parts of their cells as “pseudopods” or “false feet”

Sporozoans

• no locomotory extensions (flagella etc.)

• move by bending, creeping, and gliding

• Parasitic (part of their life-cycle is in host cell)

• “apical complex”: anterior end which helps to penetrate the host cell

Ciliates

• largest protozoa

• “hair-like” projections (cilia): “tiny oars”

• eat other protozoa and bacteria

Mycology

• Page 271

• “the branch of science concerned with the study of fungi”

Fungus (Fungi = plural)

• “a group of diverse and widespread unicellular and multicellular organisms, lacking chlorophyll, usually bearing spores and often filamentous”

• 3 fundamental categories: yeasts, molds, dimorphic (to be studied in depth later)

Saprophytes: fungi that grow on decomposing matter

Human Fungal Diseases

• categorized by the level of tissue penetration

• 1) superficial mycoses: outermost layers of skin and hair

• 2) cutaneous mycoses: skin, hair and fingernails and are deeper

• 3) subcutaneous mycoses: usually result of trauma; deep in the integument and underlying structures (muscle, bone)

• 4) systemic mycoses: usually originate in the lungs (histoplasmosis, blastoplasmosis)

Opportunistic Fungal Diseases

• due to suppressed immune systems

• secondary infection due to treatment for original disorder

• can be fatal and difficult to manage

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