Introduction to Biochemistry Bill Nye Video 1 Biochem. Intro Warm Up 1. What does inorganic mean and what does organic mean? 2. List foods that you would consider to be a carb, a protein, and a fat. 3. What is the element of life? 4. What is the molecule of life? 5. What is the function of an enzyme? 2 Inorganic vs. Organic Molecules Inorganic Molecules Organic Molecules Small Large Fast reaction time Slow reaction time Do not contain carbon Contain carbon 3 Characteristics of Organic Molecules 1.They are usually made of the SPONCH atoms. • What are the SPONCH elements? 4 2.They are formed through COVALENT bonds – electrons are shared. • Carbon has 4 electrons in it’s outer energy level so it can bond with up to 4 other atoms. This results in a huge variety of organic compounds. • Tell me the protons, neutrons, and electrons in carbon. • Draw the electron dot diagram for carbon 5 CARBON Atomic Number = 6 Atomic Mass = 12 Protons =6 Neutrons = 6 Electrons = 6 6 3.Organic molecules are large. Some are made of only 4 atoms and some are made of hundreds of atoms. 4.Organic molecules need enzymes. The reactions that join them together are very slow so enzymes are needed to speed them up. Let’s review enzymes: 7 5.They are composed of carbon skeletons. (Carbon is sometimes referred to as the element of life!). The structure is made of carbon “skeletons” bonded to hydrogen atoms. There are 3 main skeleton structures. Important Note: Chemical Formula shows the number and type of atoms present in a compound. Structural Formula shows the arrangement of the atoms 8 6.Small organic molecules called MONOMERS bond together to form long chains called POLYMERS or MACROMOLECULES. What does mono mean? What does poly mean? What does macro mean? 9 7.There are 4 main classes of important organic compounds: a) Carbohydrates b) Lipids c) Proteins d) Nucleic Acids Bill Nye Video 10 ORGANIC MOLECULES REVIEW STORY The most important elements in organic compounds are __1_ and then __2__. Organic compounds have a variety of structures called __3_. They may be ring, __4__, or __5__. The __6_ formula shows us the arrangement of the atoms and the ___7__ formula shows us the number and type of atoms present. Organic molecules consist of small units called __8__ joined together to form bigger units called _9_. Because the reactions to join the units together takes a long time to happen, __10_ are used to speed up the reactions. Carbon has _11__ electrons in its outer layer which means it can bond with _12_ other atoms. This allows for a lot of diversity in organic molecules. 11 Person A: Hey! I have some amazing news. Person B: What is it? Don’t keep me in suspense. Person A: WE ARE GOING TO LEARN ABOUT THE ORGANIC MOLECULES Person B: That’s great!!!!!!! What are the 4 types of organic molecules? Person A: Well, they are ____, _____, _____, and _____. Person B: Oh my! I can barely contain my excitement. Person A: I know! Well, luckily we get to learn about carbohydrates today Both: WOO HOOOOOOOOOOOOOOOOOO! 12 Carbohydrates 13 I. Carbohydrates • Organic compounds composed of carbon, hydrogen and oxygen with a ratio of 2 hydrogen atoms to every 1 oxygen atom (CH2O) • The basic unit of carbohydrates is a simple sugar or MONOSACCHARIDE. 14 I. Carbohydrates Cont’d • The main function of carbohydrates is ENERGY. • Examples of carbohydrates include: – – – – – Pasta Bread Candy Fruit Grains 15 Types of Carbohydrates 1.Monosaccharide • simple sugar, one sugar unit Examples: glucose fructose galactose glucose *All 3 have the chemical formula (C6H12O6) 16 Types of Carbohydrates 2.Disaccharide • Made up of 2 monosaccharides joined together Examples: sucrose lactose maltose glucose glucose 17 Types of Carbohydrates 3. Polysaccharide • Made up of many monosaccharides joined together. • Found in complex carbohydrates like pasta and potatoes. glucose glucose glucose glucose cellulose glucose glucose glucose glucose 18 Types of Carbohydrates Examples of Polysaccharides 1. Starch: energy storage for plants 2. Glycogen: energy storage for animals,found in liver and muscles. 3. Cellulose: found in plant cell walls, animals cannot digest this (“roughage”), but some bacteria can. 4. Chitin: found in the exoskeleton of insects (remember: crunchy “chitin”) 19 Vocab Review – make a “match” 1. Monomer 2. Polymer 3. Monosaccharide 4. Disaccharide 5. Polysaccharide 6. Starch 7. Cellulose 8. Chitin 9. Glycogen 10.Organic a. b. c. d. e. f. g. h. i. j. Exoskeleton Living Cell Wall Structure Basic Unit of carbs “Many units together” Energy storage in animals Energy storage in plants Many sugars joined Two sugars joined “Small unit” 20 Vocab Review – Answers 1. Monomer 2. Polymer 3. Monosaccharide 4. Disaccharide 5. Polysaccharide 6. Starch 7. Cellulose 8. Chitin 9. Glycogen 10.Organic a. b. c. d. e. f. g. h. i. j. Exoskeleton Living Cell Wall Structure Basic Unit of carbs “Many units together” Energy storage in animals Energy storage in plants Many sugars joined Two sugars joined “Small unit” 21 CARBOHYDRATES REVIEW STORY Carbohydrates are made of __1__, hydrogen and oxygen. They are made of 2 hydrogens to every _2_ oxygen. The basic unit of carbohydrates is a _____3______ or simple sugar. Two of these together is a ____4_____ and a chain of them is called a ____5___. ____6____ makes up the exoskeleton of insects. ____7_____ is found in cell walls and cannot be broken down by us. ____8____ is how plants store their energy and _____9_____ is how animals store their energy. To test for simple sugars we use the chemical reagent _____10______ and to test for starches we use the chemical reagent ____11______. 22 A: Hi (name of B), I had so much fun learning about carbs yesterday. B. Me too! Do you remember the chemical we used to test for simple sugar? A: I do! It is ___________ Reagent. Do you remember the chemical we used to test for starch? B: You bet I do! It is ____________. A. I was wondering -- What kind of food has a lot of carbs in it? B. That’s easy. (Food) has tons of carbs. A: Thanks for sharing. Are you ready? B: Ready for what? A: Ready to learn about lipids!!!!!!!!! Both: BRING – IT – ON (double high five) 23 Lipids 24 II. Lipids • Composed of carbon, hydrogen, and oxygen but the ratio of hydrogen atoms to oxygen is much larger. • Example C57H110O6 • Lipids Include: 1. 2. 3. 4. 5. Fats Oils/Waxes Cholesterol Hormones Steroids 25 II. Lipids Cont’d • Some foods rich in lipids include: butter, oil, donuts, dairy products • Lipids have several functions: 1.Long term energy storage 2.Insulation against heat loss 3.Protection against physical shock 4.Major component of cell membranes 26 Structure of Fats Triglycerides – basic unit of fat 1 glycerol (alcohol) 3 fatty acids(long carbon chain) 1 Carboxyl group (COOH) H H-C----O H-C----O H-C----O O C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH3 O C-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH3 O fatty acids C-CH2-CH2-CH2-CH H glycerol 27 Three Types of Fatty Acids 1. Saturated fatty acids: no double bonds, bad for you, solid at room temp. (butter, lard) 2. Unsaturated fatty acids: one double bond, good for you, liquid at room temp.(peanut and olive oils) C=C 3. Polyunsaturated fatty acid: many double bonds. Very good for you. C=C=C 28 Carb or Lipid? Triglyceride Monosaccharide Starch Oil CH20 Lots of hydrogens Insulation Protection Gives you energy Stores energy Glycerol Disaccharide Glucose Carboxyl Group Saturated Polysaccharide Sudan Test Benedicts Test Waxes Hormones Cell Membrane Structure Cell Wall Structure Chitin Polyunsaturated Iodine Steroid 29 Complete the Sentences 1. Lipids and carbs are similar because they both …. 2. Lipids and carbs are different because … 3. I think _____________ are more important because… 30 Lipids Review Story The basic unit of a lipid is called a ____1____. It is made up of a ___2___, ___3___, and a carboxyl group. There are 3 fatty acid chains. A ___4___ fatty acid has all single bonds. A ___5____ fatty acid has many double bonds and a ___6____ fatty acid has one double bond. The type of fatty acid that is bad for you is ____7____. Lipids have many functions. They store ____8___. They are used for ____9_____ and keep us warm. They provide _____10___ so that we don’t get hurt when we fall. If we are testing a sample to see if it has lipids we can use the simple ____11_______ test or we can use the ____12___ test and look for _13_ layers and ____14_____. An example of a lipid could be _____15_____ . 31 Proteins 32 III. Proteins (Polypeptides) 1. Proteins are composed of carbon, hydrogen, oxygen, as well as nitrogen and sometimes sulfur. 2. The basic unit for proteins is the AMINO ACIDS. There are 20 different amino acids found in proteins, each with a different chemical structure. 3. Some foods with proteins are peanut butter, eggs, meat, beans. 33 34 3. Proteins have several functions: • Build living materials -- structure • Act as enzymes • Fight disease (antibodies) • Transportation of molecules into or out of cells • Act as markers on cell 4. Proteins form when amino acids join together through PEPTIDE BONDS. Amino Acids (aa) aa1 aa2 aa3 Peptide Bonds aa4 aa5 aa6 35 5.A DIPEPTIDE is what we call two amino acids joined together. 6.A POLYPEPTIDE is what we call a chain of amino acids. They keep folding until a completely functional protein is formed. These proteins look like blobs and are called GLOBULAR PROTEINS. 36 37 Vocab Review 1. Amino Acid 2. Nitrogen 3. Antibody 4. Peptide Bond 5. Dipeptide 6. Polypeptide 7. Globular 8. Meat 9. Structure 10. Marker 11. Transportation 12. Enzyme 38 Vocab Review Continued… a. Identify blood cells b. Food containing protein c. Basic unit d. Help move things into cell e. Speed up reactions f. Fight disease g. Two amino acids h. Many amino acids i. Shape of protein j. Element in proteins k. Bond connecting amino acids l. Build living materials 39 Protein Review Story The basic unit of a protein is an _________. There are ___ different kinds of these. Two of them together is called a _________ and a chain of them is called a _________. The name of the bond that joins them together is called a _____ bond. A long chain of amino acids can fold up and look like a blob and we call it a _____ protein. Proteins are made of carbon, hydrogen, and oxygen but all proteins also contain _____. Proteins have many different functions. They help in fighting disease by acting as ____. They speed up chemical reactions by acting as _____. They tell us the identity of cells by acting as _____. However, the most important function is that they are used for ____ by building living material. Two examples of foods with proteins are _______ and _________. The chemical used in the test for proteins is _______. A positive test will turn the solution a ________ color. 40 Nucleic Acids 41 IV. Nucleic acids 1.Nucleic acids are composed of the building blocks NUCLEOTIDES. 2.They are found in all of your cells. 3. Nucleotides consist of a sugar, phosphate, and nitrogenous base. 42 Types of Nucleic Acids 1.DNA found in the nucleus of each of your cells, this is our genetic code 2.RNA made from DNA, carries the code to make proteins. 3.ATP used by all living things for energy 43 Review Game Dustbin Review Game 44