Lecture 16 Class: Transcription and Translation Starter: You Which

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Lecture 16 Class: Transcription and Translation

Starter:

You

Which of the following is NOT a nucleotide sequence?

A.

Promoter

B.

TATA box

C.

Enhancer

D.

Transcription factor

Template strand vs coding strand

3’ and 5’ ends

DNA 3’ CAT ACC GAG GAT 5’

DNA 3’ GAT CAC GAG TAT 5’

Lecture 16 Page 1

5’ CAT ACC GAG GAT 3’

A particular triplet of bases in the template sequence of DNA is 3’ – ___________ – 5’. The anticodon (3’ – 5’) on the tRNA that binds the mRNA codon is:

A.

TGG

B.

GGU

C.

GGT

D.

UGG

E.

CCA

Suppose that a single DNA base change of an C to a T occurs and is copied during replication. Is this change a mutation?

A.

Yes, it is a change in the DNA sequence

B.

Only if it alters the amino acid sequence of a protein

C.

Only if it alters the appearance of the organism

A nonsense mutation in a gene:

A.

Changes an amino acid in the encoded protein

B.

Has no effect on the amino acid sequence

C.

Introduces a stop codon

D.

Alters the reading frame of the mRNA

The following DNA sequence (coding strand) occurs near the middle of the coding region of a gene: 5’ A A T G A A C G A G A G C C T G A T 3’

Which of the following DNA mutations at the red C is almost certain to result in a shorter-thannormal mRNA?

A.

C to G

B.

C to A

C.

C to T

D.

None of the above

Lecture 16 Page 2

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