This is the base sequence on a certain DNA molecule:

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Bio 121 Transcription-translation worksheet
Name: __________________
This is the base sequence on a certain DNA molecule:
3' - A A G G T A C A T G G G G C A C T G T G G A - 5'
1. Give the base sequence of the complementary DNA strand (the other side).
2. Give the base sequence of the strand of mRNA which would be constructed from the original DNA
strand.
3. What amino acid sequence would this mRNA code for?
4. If the eighth nucleotide of the original strand of DNA were changed from A to G, what would the
resulting mRNA strand read?
5. What would the resulting amino acid sequence be? What type of mutation is this: silent, missense, or
nonsense?
6. If a the eighth base of the original DNA strand were deleted, what would the resulting mRNA read?
7. What would the resulting amino acid sequence be? What type of mutation does this represent: silent,
missense or nonsense?
8. If the following stretch of DNA were transcribed into mRNA and then translated into protein, what
would the first three amino acids in the resulting protein be? (hint: compare this sequence very carefully
with the sequence at the top of the page!).
5' - A A G G T A C A T G G G G C A C T G T G G A - 3'
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