“I like some animals more than some people, some people more than some animals” – Jane Goodall OBJECTIVE S 1. Provide examples and descriptions of animals from several different phyla. 2. Understand taxonomy and hierarchal classification. 3. Use a dichotomous key. 4. Dissect a frog and identify external and internal structures. 5. Dissect a perch and identify external and internal structures. Kingdom Animalia Kingdom Animalia consists of multicellular eukaryotes that are heterotrophs (must ingest other organisms to maintain life). Below you will find a brief description of some of the most known phyla, subphyla and classes found in Kingdom Animalia. Phylum Porifera – Phylum Porifera includes approximately 10,000 species of sponges that vary in size from a few millimeters to 2 meters across. Sponges have bodies that are organized around a system of water canals and chambers. Phylum Cnidaria – Phylum Cnidaria includes nearly 10,000 species of mainly marine invertebrates including sea anemones, jellyfish, Portuguese man-of-war, coral and freshwater Hydras. Cnidarians exhibit radial symmetry and are diploblastic (having only 2 germs layers during development). Phylum Platyhelminthes – A flattened, ribbon-like body is a common characteristic of members of phylum Platyhelminthes. Planarians, liver flukes and tapeworms are all member of this phylum that lack specialized respiratory and circulatory systems. Phylum Nematoda – Phylum Nematoda consists of round worms. Common examples of Phylum Nematoda are ascaris, hookworms, pinworms, Guinea worms, eye worms, heartworms and whipworms. Member of this phylum range from microscopic to 30cm in length. Their bodies are generally cylindrical and tapered on both ends. Phylum Arthropoda – Common examples of Phylum Arthropoda are butterflies, fleas, lobsters, spiders, ticks and barnacles. More than 1,000,000 species have been identified and this number continues to grow. Arthropods are distinguished by their chitinous exoskeleton that provides support and protection to the arthropods. Phylum Mollusca – Phylum Mollusca exhibits great diversity, but they share a body plan that is bilaterally symmetrical. Members of phylum Mollusca include cuttlefish, octopi, snails, clams, mussels, scallops and squid. Phylum Echinodermata – Echinoderms, members of Phylum Enchinodermata, have five-pointed (pentamerous) symmetry. Some examples include sea lilies, starfish, brittle stars, sea urchins, sand dollars and sea cucumbers. Phylum Chordata – fundamental characteristics of chordates area notochord, dorsal nerve cord, pharyngeal pouches and a postanal tail. Subphylum Urochordata – This subphylum is composed of nearly 3,000 species of marine tunicates (sea squirts), salps and larvaceans. Subphylum Cephalochordata – Members of this subphylum are often referred to as sea lancelets because of their slender bodies. Subphylum Vertebrata (Craniata) - This subphylum is made up of more than 55,000 species that follow the general body plan of chordates. In the majority of vertebrates, the vertebral column surrounds or replaces the notochord. Superclass Agnatha – This superclass consists of about 70 species of jawless fish that lack scales, internal ossification and paired fins while possessing eel-like bodies. Hagfish and lampreys are members of Superclass Agnatha. Class Chondrichthyes – Member of this class are cartilaginous fishes that include sharks, rays, skates and sawfish. The entire skeleton of members of Class Chrondrichthyes is made of cartilage. This class was the first set of vertebrates to have jaws and paired appendages. Their jaws, paired appendages, more efficient respiration and better nervous system allowed them to attack and eat larger prey. Class Osteichthyes – Class Osteichthyes includes nearly 27,000 species of bony fish and is the largest group of all vertebrates. Their gills are covered by a bony operculum, they have flat and bony scales and most have a swim bladder that acts as a floatation device. Class Amphibian – Class Amphibian includes nearly 6,000 species including frogs, toads, salamanders and newts. Amphibians evolved from fish and developed a protective integument (skin) that allows them to breathe on land. They still, however, require water for their reproduction. INSERT FIGURE 30.1 (Pg. 602) from Pendarvis HERE Class Reptilia – Approximately 7,500 species of reptiles make up Class Reptilia and they are the oldest group of vertebrates living exclusively on land. Common examples includes turtles, lizards, snakes and crocodiles. These animals are ectothermic and therefore rely on the environment for their temperature regulation. Additionally, they produce amniotic eggs. Class Aves – Birds (Class Aves) evolved from the dinosaur lineage and consists of nearly 9,700 species. Their most distinguishing characteristic is the presence of feathers. They are endothermic with a four chambered heart and have hollow bones. Class Mammalia – Class Mammalia consists of approximately 5,000 species that live in marine, freshwater, aerial and terrestrial habitats. Humans are a member of Class Mammalia. One of the most obvious characteristics of mammals is the presence of hair and mammary glands. Additionally, mammals have specialized teeth and in-folding of the brain that generally make the brain larger in mammals than in any other group of animals. Anatomical and Morphological Terms Animals come in an enormous variety of shapes and sizes and have varying degrees of complexity and similarity. Below is a list of terms used to describe animals: Appendages: These are extensions from the animal’s body. The purposes are for movement, feeding, or other purposes. Examples include legs and arms. Asymmetric: Irregular organization, not the same on any side. Bilateral symmetry: The body parts are arranged so that they are a mirror image on the left and right sides. Humans are bilaterally symmetrical. Radial symmetry: The animal looks the same from all sides. The body is arranged like a circle around a central axis. Examples include sea stars and jellies. Exoskeleton: The animal has a hard, external skeleton that protects it and provides points of attachment for muscles. Animals with exoskeletons do not have internal bones. Examples include shrimp and crabs. Endoskeleton: The animal has a hard skeleton composed of cartilage and/or bone within the soft tissues. Cats and fish have internal skeletons. Hydrostatic skeleton: The animal is supported by a fluid filled coelom (inner cavity). Sea urchins, earthworms, and jellies have hydrostatic skeletons. Segmentation: The animal body is subdivided into a series of repeated parts called segments. An earthworm is segmented because it is divided into many compartments. An example of internal segmentation is the vertebral column (backbone). Microscopic: Animals too small to be seen without a microscope. Macroscopic: Animals large enough to be seen without a microscope. Larva: Distinctive juvenile form. Pores: Small openings for passage of water. The porifera (sponges) contain many incurrent (waterentering) pores and one main excurrent (water exiting) pore. Procedure 1 – Observing and understanding animal morphology Observe the different animal groups on the display table. As you observe them, note their distinctions and sketch them. These can be done in any order. List two animals with radial symmetry Common Name Scientific name 1. 2. Sketch one of these animals. Common name_____________________________________________ Phylum List two animals with bilateral symmetry Common Name Scientific name Phylum 1. 2. Sketch one of these animals. Common name_____________________________________________ List two adult animals or larva that are microscopic Common Name Scientific name 1. 2. Sketch one of these animals. Common name_____________________________________________ Phylum List two animals that are segmented Common Name Scientific name Phylum 1. 2. Sketch one of these animals. Common name_____________________________________________ List two animals with an endoskeleton Common Name Scientific name 1. 2. Sketch one of these animals. Common name_____________________________________________ Phylum List two animals with a hydrostatic skeleton Common Name Scientific name Phylum 1. 2. Sketch one of these animals. Common name_____________________________________________ Check Your Understanding How many appendages are on an alligator? ______________________________________________________________ Why is a human considered “segmented”? _______________________________________________________________ __________________________________________________________________________________________________ What are the differences between an endoskeleton, an exoskeleton, and a hydrostatic skeleton? __________________________________________________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ Procedure 2 – Animal Classification Kingdom Phylum Class Order Family Genus Species Taxonomy is the classification of organisms based on shared characteristics. Organisms can be classified using different systems. Classical taxonomy groups organisms primarily based on morphology. During the mid-1800s Carolus Linnaeus developed a scheme where he assigned each organism a two part scientific name, genus and species. All organisms are divided into a taxonomic hierarchy. As you go down the hierarchy, the members are increasingly similar to one another. Phylogenetic systematics is the classification of organisms based on DNA sequence information. Increasingly, taxonomists are regrouping organisms based on DNA. One way to identify organisms is by using a dichotomous key, formatted as a series of paired choices. For each choice, only one will describe the specimen. Each selection will direct the user to find a reference to the next set of choices. The choices will guide the user to identify the organism. KEY TO ANIMAL PHYLA Characteristics 1. Radially symmetric or asymmetric adult 1b. Bilaterally symmetric Next Step 2 4 2. Body contains pores 2b. Body does NOT have pores Porifera 4 3. Radial symmetry, with soft body and stinging cells 3b. Radial symmetry in adult, but bilateral symmetry in larva; Adults have tube feet, body plan usually pentamerous (5 parted) 4. Body unsegmented 4b. Body segmented 5. Body thin & flat; usually parasitic or aquatic; mouth & anus are a single opening 5b. Body not flat Phylum Cnidaria Enchinodermata 5 7 Platyhelminthes 6 6. Body is pencil or spindle-shaped 6b. Body is soft; most have shells over body and move with a large muscular “foot”; others have tentacles modified from the ancestral foot Nematoda Mollusca 7. Body is worm-like in adult without a distinct head, but with a separate mouth and anus; paired appendages which are NOT jointed 7b. Body is NOT worm-like & has a distinct head 8 Annelida 8. Skeleton is external (exoskeleton); 1 or 2 pairs of jointed appendages per body segment 8b. Skeleton is internal (endoskeleton); only 2 pars of jointed appendages Arthropoda Chordata Procedure 3 – Classification of Unknowns Classify the unknown animal specimens provided into their correct phylum by using the dichotomous key given on the previous page. Number A 1 2 3 4 5 6 7 8 9 10 Steps 1b, 4b, 7b, 8 Description 5 pairs of jointed appendages, pinchers, exoskeleton Phylum Arthropoda Common Name Crab Procedure 4 – Frog Dissection 1. Follow all of your instructor’s directions carefully. 2. Obtain a set of protective goggles and gloves. 3. Put your gloves and googles on properly. Goggles should NEVER be worn on your forehead or around your neck. They aren’t protecting your eyes unless you look a little goofy…better to look goofy than never be able to see yourself again. 4. Obtain a doubly injected bullfrog and place it in a dissecting tray. 5. Locate the dorsal and ventral external anatomical features shown in the figure below. 6. Using a probe, carefully pry open the mouth and observe the structures shown in the figure below. INSERT FIGURE 30.42 External anatomy of a bullfrog Pg. 639 Pendarvis 7. Using sharp scissors, cut through the skin and muscle with a shallow incision where the legs meet the body. Lift the tissue as much as possible to avoid damaging the organs underneath. 8. Continue this incision up to the jaw. It is easiest to cut a couple of millimeters to the left or right of the midline (also known as linea alba). You will have to carefully cut through the pectoral girdle. 9. Make 2 sets of lateral cuts through the body wall to each side; one set is immediately behind the pectoral girdle and the other at the other end of the trunk incision. 10. Fold and pin down the muscles flaps you have created. 11. Identify the internal organs and sex of the bullfrog using the figures on the next page. 12. Be sure to observe a frog of the opposite sex from another lab group. 13. Discard of the bullfrog in the labeled bucket as indicated by your instructor. 14. Thoroughly clean all instruments, your dissecting tray and your lab bench. 15. Return all equipment (including goggles) to the proper locations. INSERT FIGURE 30.47, 30.48, 30.49 Pg. 641 and 642 Pendarvis Procedure 5 – Perch Dissection 1. Follow all of your instructor’s directions carefully. 2. Obtain a set of protective goggles and gloves. 3. Put gloves and googles on properly. Goggles should NEVER be worn on your forehead or around your neck. They aren’t protecting your eyes unless you look a little goofy…better to look goofy than never be able to see yourself again. 4. Obtain a perch from your instructor. 5. Identify the external anatomical structures shown in the figure and listed below. 1. Spiny dorsal fin 8. Pelvic fin 2. Eye 9. Lateral line 3. External nares 10. Soft dorsal fine 4. Upper jaw (maxilla) 11. Caudal fin 5. Lower jaw (mandible) 12. Anal fin 6. Operculum 13. Anus 7. Pectoral fin INSERT FIGURE External Anatomy of a perch with callouts 6. Use a probe to lift the operculum and observe the gills. 7. Locate the anus (in front of the anal fin). A male perch has only one pore (urogenital pore) behind the anus. A female has a genital pore and a urinary pore behind the anus. Determine the sex of your fish. Be sure to look for the opposite sex perch at another lab bench. 8. Use dissecting pins to secure the fish to the dissecting tray. 9. Use scissors to make the cuts shown in the figure from Carolina Biological Supply 10. Using the figure below, identify the viscera of the perch. INSERT FIGURE Anatomy of a female perch AND viscera of a perch with callouts 11. Discard of the perch in the labeled bucket as indicated by your instructor. 12. Thoroughly clean all instruments, your dissecting tray and your lab bench. 13. Return all equipment (including goggles) to the proper locations. Animal Diversity Review 1. Label the internal organs in this ventral view of the bullfrog 1. _____________________________ INSERT FIGURE Ventral view of frog viscera with callouts 2. _____________________________ 3. _____________________________ 4. _____________________________ 5. _____________________________ 6. _____________________________ 7. _____________________________ 8. _____________________________ 9. _____________________________ 10. _____________________________ 11. _____________________________ 12. _____________________________ 2. Label the internal organs in the perch 1. _____________________________ INSERT FIGURE Viscera of a perch with callouts 2. _____________________________ 3. _____________________________ 4. _____________________________ 5. _____________________________ 6. _____________________________ 7. _____________________________ 8. _____________________________ 9. _____________________________ 10. _____________________________ 11. _____________________________ 12. _____________________________ 3. Who created the binomial system for naming organism in the 18 th century? ______________________ ____________________________________________________________________________________ 4. Why are scientific names important in biology? _____________________________________________ ____________________________________________________________________________________ ____________________________________________________________________________________ ____________________________________________________________________________________ 5. List the 7 levels of the taxonomic hierarchy from largest to smallest. 1. _____________________________ 2. _____________________________ 3. _____________________________ 4. _____________________________ 5. _____________________________ 6. _____________________________ 7. _____________________________ 6. Complete the following chart: Phylum Porifera Cnidaria Enchinodermata Platyhelminthes Nematoda Mollusca Annelida Arthropoda Chordata Characteristics List 1 animal found in this phylum