FST 305 GENERAL MICROBIOLOGY By Prof. Olusola Oyewole And Dr. Olusegun Obadina Know Your Lecturers Prof. Olusola Oyewole Dr. Olusegun Obadina Class organization 1. 2. 3. 4. 5. 6. Learners Introduction. Explanation of the Learner- Based learning. Expectations. Lectures Practical Assessment Course Content • Historical development and scope of microbiology. • Functional classification and morphology of microorganisms, microbial nomenclature-fungi, algae, bacteria, viruses, protozoa, Rickettsia and cultivation and isolation of micro-organism – • Use of microscopy, culture media, staining methods, maintenance of cultures. • Microbial physiology and biochemistry; reproduction; useful and harmful micro-organisms. • Public health considerations of micro-orgnisms. This Course LECTURES • Basic knowledge of the diversity of microorganisms. These are; bacteria, viruses, protozoa and fungi. Their biology as it relates to their economic importance in the environment are discussed in detail. PRACTICALS • The theory aspects of the module will be elaborated through relevant laboratory exercises to illustrate the principles and concepts of the subject matter What Is Needed To Benefit from the Course • • • • ● Attendance in Lectures – Learner Based Approach Relevant reference text books. Students Home assignments and ● Laboratory equipment such as a microscope, an autoclave, oven, an incubator (various temperatures), Petri plates, materials for bacterial and fungal media preparations, various staining chemicals, identification manuals and other laboratory equipment and materials for standard microbiology work • ● Capacity to facilitate for site visits/field trips. Learning Issues • • • • • • • • • • • Culture - pure culture and mixed culture. Morphological shapes of bacteria The Gram stain technique Classification of Bacteria Factors that affect bacterial growth Phases of microbial growth Differentiate between: ● Mesophile and thermophile. ● Preservation and pasteurisation. ● Sporulation and germination. ● Selective medium and differential medium Practical Reporting 1. Title: The title should be concise. It is a summary of the body of work that covers the contents of the task under investigation. 2. Introduction: A brief statement of the background to the subject, its importance and justification for the study. 3. Objective(s): A statement of the main expected outcomes (achievements) from the study. 4. Methods: An explanation of the details of how the objectives are going to be achieved. This is important because someone else should be able to use the description of your methods in order to repeat the outcomes of your work 5. Results: Results are better presented in form of pictures tables, graphs, or charts. These should carry a short and clear title, with a description of the results. 6. Discussion: An opportunity is provided for the interpretation of results focusing on the objectives. Comparisons and similarities in the results with other published works are discussed and acknowledged. 7. Conclusions: A statement is made as to whether the objectives of the exercise had been achieved. 8. Recommendations: Suggestions are made about what needs to be investigated further arising from some inconclusive results from the work. 9. References: all references used in the write up should be acknowledged in this part using an accepted international Further Reading • http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookDiv ersity 2.html • ● http://en.wikipendia.org/wiki/Bacteria • ● http://en.wikipedia.org/wiki/Microscopy • ● http://en.wikipedia.org/wiki/Category:Laboratory_techniques • http://en.wikipedia.org/wiki/Category:Microbiology_techniqu es • http://en.wikipedia.org/wiki/Deer_Island_Waste_Water_Treatment _Plant • ● Frobisher, M., Hinsdill, R. D., Crabtree, K. T. & Goodheart, C. R. 1974. Fundamentals of Microbiology. Nonth Edition. Philadelphia: Saunders College Publishing. • ● Any other Microbiology book Acknowledgment • • Materials Used have been obtained from the Microbiology and Mycology Modules of the AVU as prepared by Prof. Jassiel Nyengani Zulu, University of Zambia, Department of Biological Sciences, Lusaka and Dr. Modest Diamond Varisanga, Open University of Tanzania, Faculty of Science, Technology and Environmental Studies. However, these have been modified to fit into the Course contents of the University of Agriculture, Abeokuta, Nigeria.